##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364418_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1792499 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.617161850578437 31.0 31.0 34.0 27.0 34.0 2 30.607041342840358 31.0 31.0 34.0 26.0 34.0 3 30.52636235780327 31.0 31.0 34.0 26.0 34.0 4 34.03695622703276 37.0 35.0 37.0 30.0 37.0 5 34.25601241618545 37.0 35.0 37.0 30.0 37.0 6 34.343879131871205 37.0 35.0 37.0 30.0 37.0 7 34.20054906585722 37.0 35.0 37.0 30.0 37.0 8 34.05492778517589 37.0 35.0 37.0 30.0 37.0 9 35.09167257554955 39.0 35.0 39.0 27.0 39.0 10-11 35.33576615663384 39.0 35.0 39.0 29.0 39.0 12-13 35.40440162030774 39.0 35.0 39.0 29.5 39.0 14-15 36.54184716421041 40.0 36.0 41.0 29.0 41.0 16-17 36.721788408250156 40.0 36.5 41.0 30.0 41.0 18-19 36.73226372790166 40.0 36.5 41.0 30.0 41.0 20-21 36.68933873882217 40.0 37.0 41.0 29.5 41.0 22-23 36.61803800169484 40.0 36.0 41.0 29.0 41.0 24-25 36.70776050642148 40.0 36.0 41.0 29.5 41.0 26-27 36.733553268370024 40.0 36.0 41.0 30.0 41.0 28-29 36.69892368140791 40.0 36.0 41.0 30.0 41.0 30-31 36.4773324838675 40.0 36.0 41.0 29.0 41.0 32-33 36.552726110307454 40.0 36.0 41.0 30.0 41.0 34-35 36.50609595877041 40.0 36.0 41.0 30.0 41.0 36-37 36.39202225496361 40.0 36.0 41.0 29.0 41.0 38-39 36.26128829081634 40.0 35.5 41.0 28.5 41.0 40-41 36.233778652038296 40.0 35.0 41.0 29.0 41.0 42-43 36.11789434750033 40.0 35.0 41.0 28.0 41.0 44-45 36.11603576905762 40.0 35.0 41.0 28.0 41.0 46-47 35.991073635187526 39.5 35.0 41.0 28.0 41.0 48-49 35.84140521138366 39.0 35.0 41.0 28.0 41.0 50-51 35.732200408480004 39.0 35.0 41.0 27.5 41.0 52-53 35.62381401607476 39.0 35.0 41.0 28.0 41.0 54-55 35.521154823517335 39.0 34.5 41.0 28.0 41.0 56-57 35.49934225904728 39.0 34.5 40.5 28.0 41.0 58-59 35.48794253162763 39.0 35.0 41.0 28.0 41.0 60-61 35.32152123934239 38.5 34.0 41.0 28.0 41.0 62-63 35.078858900339696 38.0 34.0 40.0 28.0 41.0 64-65 34.78883865486117 37.5 34.0 40.0 27.0 41.0 66-67 34.49333862947762 37.0 34.0 40.0 26.5 41.0 68-69 34.19515045754558 37.0 34.0 39.5 26.0 41.0 70-71 33.84477536668082 36.0 33.0 39.0 26.0 41.0 72-73 33.50499191352408 36.0 33.0 39.0 26.0 41.0 74-75 33.14002685636087 35.0 33.0 38.5 26.0 40.0 76-77 32.777283557759304 35.0 33.0 37.0 25.5 39.0 78-79 32.444448504573785 35.0 32.5 37.0 25.0 39.0 80-81 32.110443018378255 35.0 32.0 36.0 25.0 39.0 82-83 31.807416350023068 35.0 32.0 36.0 24.0 37.0 84-85 31.438921025897365 35.0 32.0 35.5 22.5 37.0 86-87 31.229229974465817 35.0 32.0 35.0 22.5 37.0 88-89 31.071704921453232 35.0 32.0 35.0 22.0 36.0 90-91 30.92624291561669 35.0 32.0 35.0 20.5 36.0 92-93 30.693629954605274 35.0 31.0 35.0 20.0 36.0 94-95 30.599292105602288 35.0 31.0 35.0 19.5 35.5 96-97 30.50873278032512 35.0 31.0 35.0 19.0 35.0 98-99 30.349809121232425 34.5 31.0 35.0 18.5 35.0 100-101 30.21737920076943 34.0 31.0 35.0 17.5 35.0 102-103 29.221626622943724 33.5 29.5 34.5 15.0 35.0 104-105 30.1207281008246 34.0 31.0 35.0 17.5 35.0 106-107 30.342025853291968 35.0 31.5 35.0 17.5 35.0 108-109 30.325531841300887 35.0 32.0 35.0 16.0 35.0 110-111 30.21046789984262 35.0 31.5 35.0 10.0 35.0 112-113 30.08491552854423 35.0 31.0 35.0 6.0 35.0 114-115 29.97539161807064 35.0 31.0 35.0 2.0 35.0 116-117 29.847431156168007 35.0 31.0 35.0 2.0 35.0 118-119 29.57920506510743 35.0 31.0 35.0 2.0 35.0 120-121 29.438141945964823 35.0 31.0 35.0 2.0 35.0 122-123 29.19217249214644 35.0 31.0 35.0 2.0 35.0 124-125 28.035038234330955 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8570767447627965 1101 2 0.8345076891692962 1101 3 0.9584955032133244 1101 4 0.8787741907239734 1101 5 0.7010902115264415 1101 6 0.6704944661238983 1101 7 0.7368544557647994 1101 8 0.8301412588298334 1101 9 1.400114277242153 1101 10-11 0.9938288385683762 1101 12-13 0.9107361455693166 1101 14-15 1.1239213674146384 1101 16-17 0.961570284771966 1101 18-19 1.0441592175289287 1101 20-21 1.0825042674067475 1101 22-23 1.0636066388187757 1101 24-25 1.084213914629025 1101 26-27 0.9647383757049042 1101 28-29 1.0444510763982322 1101 30-31 1.204035197158177 1101 32-33 1.0589452889279372 1101 34-35 0.981586138621978 1101 36-37 1.009642938655034 1101 38-39 1.1546030109804235 1101 40-41 1.039318967554408 1101 42-43 1.0948963002505039 1101 44-45 0.8440618116124767 1101 46-47 0.8658728358603724 1101 48-49 0.823653297838618 1101 50-51 0.8656622964927365 1101 52-53 0.827478743931735 1101 54-55 0.6879443504430967 1101 56-57 0.6940087846338372 1101 58-59 0.837109299245185 1101 60-61 0.9060393933105857 1101 62-63 0.8522566677987058 1101 64-65 0.7775198011719056 1101 66-67 0.7914397479944171 1101 68-69 0.8382022786799084 1101 70-71 0.833088038638806 1101 72-73 0.8261215442981467 1101 74-75 0.8778668046995364 1101 76-77 0.8145430174770638 1101 78-79 0.7111098754253433 1101 80-81 0.8251463521119362 1101 82-83 0.8823393660749446 1101 84-85 1.017710536658246 1101 86-87 0.9348394332188619 1101 88-89 0.8154549686088082 1101 90-91 0.8052777312829278 1101 92-93 0.9286842131256456 1101 94-95 0.8590684550838432 1101 96-97 0.8123937563814891 1101 98-99 0.9157408663247644 1101 100-101 0.8424336307287206 1101 102-103 0.7360920411067475 1101 104-105 0.6959989225488563 1101 106-107 0.7413248331527065 1101 108-109 0.7581052817118987 1101 110-111 0.8213034034604725 1101 112-113 0.7153682345247496 1101 114-115 0.6552290146359852 1101 116-117 0.7491027105871595 1101 118-119 0.7802660390254665 1101 120-121 0.7038783537063935 1101 122-123 0.7414984345498965 1101 124-125 0.540311225093614 1102 1 0.37314168866765485 1102 2 0.27659269395474695 1102 3 0.40138556917305834 1102 4 0.23508942918447673 1102 5 0.13982229200491503 1102 6 0.09504986849900376 1102 7 0.24979244532318745 1102 8 0.2607778099892286 1102 9 0.6316298308167276 1102 10-11 0.3921534129743378 1102 12-13 0.38109469148372455 1102 14-15 0.49251603466178295 1102 16-17 0.28546747189513866 1102 18-19 0.2774707831137704 1102 20-21 0.39054174939651887 1102 22-23 0.4229655613497769 1102 24-25 0.38262314139022635 1102 26-27 0.30651414025387425 1102 28-29 0.2540831323448032 1102 30-31 0.37743051674672756 1102 32-33 0.27336141226468413 1102 34-35 0.2009093344038746 1102 36-37 0.19864227871218532 1102 38-39 0.22894056311072575 1102 40-41 0.1738585105699002 1102 42-43 0.2394479710115789 1102 44-45 0.16398586635664003 1102 46-47 0.23174307934819183 1102 48-49 0.16257610525623534 1102 50-51 0.09987152256289278 1102 52-53 0.11810202713014206 1102 54-55 0.14994926373490358 1102 56-57 0.16649158638493589 1102 58-59 0.12429707890028396 1102 60-61 0.20272413066369666 1102 62-63 0.22410820484360983 1102 64-65 0.2568456229044145 1102 66-67 0.145749243915553 1102 68-69 0.17151211666566013 1102 70-71 0.28284531096716137 1102 72-73 0.1507584893501246 1102 74-75 0.21306559952640924 1102 76-77 0.18251263937472118 1102 78-79 0.14346296903702438 1102 80-81 0.2277747213620529 1102 82-83 0.18992456262388657 1102 84-85 0.29282827253598853 1102 86-87 0.17513429983093687 1102 88-89 0.16240005514424283 1102 90-91 0.03847009253080458 1102 92-93 0.05875411178468326 1102 94-95 0.16089314523894416 1102 96-97 0.05945942933315962 1102 98-99 0.026402916846674174 1102 100-101 0.010516237606637446 1102 102-103 0.042247822171571414 1102 104-105 0.05485096750789609 1102 106-107 0.0017541760776715876 1102 108-109 -0.021970663543942948 1102 110-111 -0.11708004752235723 1102 112-113 -0.01044935174290984 1102 114-115 -0.07805318176439258 1102 116-117 -0.07117031917245953 1102 118-119 -0.03344349628492793 1102 120-121 -0.15012915789443326 1102 122-123 -0.13474880561056324 1102 124-125 -0.46493272917338047 1103 1 0.5756057304421347 1103 2 0.5506095249685679 1103 3 0.6641051615208013 1103 4 0.5788584298961652 1103 5 0.48363160816441564 1103 6 0.3344708344516434 1103 7 0.3053962240050936 1103 8 0.5755525988473522 1103 9 1.0151169524994401 1103 10-11 0.6328532288122801 1103 12-13 0.5660450993690915 1103 14-15 0.6814040550773086 1103 16-17 0.6106916408689784 1103 18-19 0.5934463278179862 1103 20-21 0.5732433369201289 1103 22-23 0.6570046207108433 1103 24-25 0.594290623964767 1103 26-27 0.6925294793218768 1103 28-29 0.6384173583773389 1103 30-31 0.8356408125441845 1103 32-33 0.7904526252797197 1103 34-35 0.768438052304063 1103 36-37 0.7262534924402786 1103 38-39 0.7847136255352254 1103 40-41 0.8478094518807566 1103 42-43 0.8369783070147818 1103 44-45 0.6916192947192386 1103 46-47 0.6919122396761423 1103 48-49 0.7386137138096984 1103 50-51 0.6902523115663826 1103 52-53 0.6331629322530858 1103 54-55 0.6550047386138473 1103 56-57 0.6122597670749315 1103 58-59 0.7156127337726303 1103 60-61 0.7106446077572599 1103 62-63 0.7127526870437961 1103 64-65 0.6445991885589422 1103 66-67 0.6058065083067348 1103 68-69 0.6820716786259808 1103 70-71 0.71136362227098 1103 72-73 0.5447788445374471 1103 74-75 0.6589156452583893 1103 76-77 0.5566933375998815 1103 78-79 0.5396922405204307 1103 80-81 0.596965834411229 1103 82-83 0.45189982758825664 1103 84-85 0.569500095191902 1103 86-87 0.562604971713931 1103 88-89 0.49392536405793663 1103 90-91 0.5620905568715209 1103 92-93 0.5027083030142911 1103 94-95 0.48848420124313563 1103 96-97 0.5460046971360661 1103 98-99 0.4839083779443243 1103 100-101 0.5569736890395518 1103 102-103 0.4513933952200091 1103 104-105 0.4534371157233892 1103 106-107 0.44531871987546623 1103 108-109 0.4691772845506037 1103 110-111 0.5476106822558116 1103 112-113 0.4910682368996504 1103 114-115 0.36770046342401486 1103 116-117 0.4771230621006026 1103 118-119 0.32975487628740296 1103 120-121 0.2918810471247042 1103 122-123 0.3698808887736611 1103 124-125 0.23651759849247966 1104 1 0.6885731879230619 1104 2 0.7751565769641111 1104 3 0.8236493541273049 1104 4 0.6204864923369087 1104 5 0.6815383032909352 1104 6 0.6402283134609803 1104 7 0.7136352046969989 1104 8 0.8198966355706006 1104 9 1.202688285417338 1104 10-11 1.020856128413584 1104 12-13 0.8828797084748174 1104 14-15 0.9940340533743495 1104 16-17 0.9034100899601043 1104 18-19 0.9766089794202486 1104 20-21 1.010545193674318 1104 22-23 1.088796857152495 1104 24-25 1.1087519266748913 1104 26-27 1.0356596723380633 1104 28-29 1.0230316997989135 1104 30-31 1.1329313154053295 1104 32-33 1.1441365816917752 1104 34-35 0.9915279493903455 1104 36-37 0.8675227616052723 1104 38-39 1.0873227404416426 1104 40-41 1.0164399300261948 1104 42-43 0.9656540522978645 1104 44-45 0.8689535695773714 1104 46-47 0.8483066513045614 1104 48-49 0.9100867134315962 1104 50-51 0.9582214598516785 1104 52-53 0.790428857052909 1104 54-55 0.7963044368599199 1104 56-57 0.8506766776015553 1104 58-59 0.9807786957350544 1104 60-61 0.9351724982028458 1104 62-63 0.9741430814937431 1104 64-65 0.9897977494455787 1104 66-67 0.9518770546564994 1104 68-69 0.932845292470013 1104 70-71 0.9876045552819335 1104 72-73 0.9308087117263142 1104 74-75 0.9948663293662037 1104 76-77 0.9786135300142291 1104 78-79 0.7209528114212631 1104 80-81 0.873154950273026 1104 82-83 0.7878762853301566 1104 84-85 0.8180787669834224 1104 86-87 0.8003316616209517 1104 88-89 0.7711915074613565 1104 90-91 0.7593264628093586 1104 92-93 0.7933245816415777 1104 94-95 0.7875692102450174 1104 96-97 0.804989844775811 1104 98-99 0.8237079697223457 1104 100-101 0.82129816209018 1104 102-103 0.6630004636189142 1104 104-105 0.7382506832882321 1104 106-107 0.7169404374142481 1104 108-109 0.5960090005321703 1104 110-111 0.7188761434001023 1104 112-113 0.7187397010606702 1104 114-115 0.7479151375548732 1104 116-117 0.693155773835052 1104 118-119 0.7224058686262893 1104 120-121 0.5760330689246089 1104 122-123 0.696641180752497 1104 124-125 0.4290730259745139 1105 1 0.41757658445478896 1105 2 0.4360916259724661 1105 3 0.4638370730537318 1105 4 0.38479367266472764 1105 5 0.29543732956333457 1105 6 0.37414179118903945 1105 7 0.3841072317037373 1105 8 0.4927498683402831 1105 9 0.8981658823637062 1105 10-11 0.49835447747442885 1105 12-13 0.5979515319249984 1105 14-15 0.33299995448196995 1105 16-17 0.39849186236338596 1105 18-19 0.47684451383769044 1105 20-21 0.6958143164763868 1105 22-23 0.7419299174822811 1105 24-25 0.7305641464564943 1105 26-27 0.7263946418737532 1105 28-29 0.5734046433015578 1105 30-31 0.8256479291613417 1105 32-33 0.6331321525068248 1105 34-35 0.5625338500996975 1105 36-37 0.641518662802099 1105 38-39 0.6733047637928422 1105 40-41 0.7069888254324894 1105 42-43 0.8308518798727036 1105 44-45 0.7198231073027728 1105 46-47 0.6904294118687915 1105 48-49 0.49891700283440343 1105 50-51 0.5919781775609323 1105 52-53 0.49641078471623246 1105 54-55 0.5342117843570477 1105 56-57 0.4855544951731332 1105 58-59 0.5281734833577474 1105 60-61 0.5147648342245361 1105 62-63 0.6249974126050901 1105 64-65 0.6253543044078569 1105 66-67 0.5404025652386011 1105 68-69 0.5281073589376533 1105 70-71 0.6666703234997726 1105 72-73 0.6048495086408252 1105 74-75 0.5740989306915836 1105 76-77 0.6592820701805735 1105 78-79 0.5924779352433163 1105 80-81 0.5239602736741276 1105 82-83 0.5252786853114344 1105 84-85 0.7055404481909839 1105 86-87 0.6618767337793088 1105 88-89 0.6346837632733298 1105 90-91 0.570109568519765 1105 92-93 0.4684436774894074 1105 94-95 0.39867356860801095 1105 96-97 0.4402431726126892 1105 98-99 0.4757533562929659 1105 100-101 0.35154180282254543 1105 102-103 0.32723286516352346 1105 104-105 0.2965561518374962 1105 106-107 0.3518782538711953 1105 108-109 0.4437845224610548 1105 110-111 0.39462393446484256 1105 112-113 0.4157601357241454 1105 114-115 0.21438106226118592 1105 116-117 0.2750841871477192 1105 118-119 0.1701871835510005 1105 120-121 0.18057479058486336 1105 122-123 0.2536243462807626 1105 124-125 0.052771072790971374 1106 1 0.4772134177746672 1106 2 0.511840237340369 1106 3 0.5488859388414333 1106 4 0.4631149967934576 1106 5 0.5307385318726432 1106 6 0.3084513552379917 1106 7 0.3415770698073288 1106 8 0.5369906583176558 1106 9 0.9746935078744343 1106 10-11 0.5746415499801643 1106 12-13 0.64569423981618 1106 14-15 0.8158380763066049 1106 16-17 0.614698494465074 1106 18-19 0.6555319733115113 1106 20-21 0.7957278606196496 1106 22-23 0.7662486869966969 1106 24-25 0.6384188693680741 1106 26-27 0.6648777191814403 1106 28-29 0.6710848529830571 1106 30-31 0.7543645544663491 1106 32-33 0.7906823919530908 1106 34-35 0.6580254506007179 1106 36-37 0.5575260818604093 1106 38-39 0.7980299018748269 1106 40-41 0.7906555693538877 1106 42-43 0.7418622415550544 1106 44-45 0.519478130205755 1106 46-47 0.5636854514093272 1106 48-49 0.582964660323384 1106 50-51 0.648989695460159 1106 52-53 0.6819726566417756 1106 54-55 0.5507235509470618 1106 56-57 0.5412482773825005 1106 58-59 0.5882305520354691 1106 60-61 0.5429914345042164 1106 62-63 0.6563133775123475 1106 64-65 0.6231600964294373 1106 66-67 0.5064672743224605 1106 68-69 0.5210140725474233 1106 70-71 0.529944422418815 1106 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-2.0855953652843766 2216 40-41 -3.050053823063841 2216 42-43 -2.7417298078857257 2216 44-45 -2.513568939116354 2216 46-47 -2.3098788529167393 2216 48-49 -2.160879461681162 2216 50-51 -2.197094188214969 2216 52-53 -2.5230616457368313 2216 54-55 -2.3537940587576855 2216 56-57 -2.5251666371881853 2216 58-59 -2.5993676528519103 2216 60-61 -2.6794336068484554 2216 62-63 -2.4387884572889718 2216 64-65 -2.4759391425828383 2216 66-67 -2.7366366903570594 2216 68-69 -2.371259942199025 2216 70-71 -2.3154095342490137 2216 72-73 -2.3463861590346795 2216 74-75 -2.1505009124590515 2216 76-77 -2.0759932409367323 2216 78-79 -2.1375274758067206 2216 80-81 -2.071643498180766 2216 82-83 -2.2612420769875605 2216 84-85 -2.3865271042685485 2216 86-87 -2.4469129031261048 2216 88-89 -2.707888838595924 2216 90-91 -2.5024918790294954 2216 92-93 -2.2498542984694296 2216 94-95 -2.3236036557148907 2216 96-97 -2.345986132771621 2216 98-99 -2.2871267440674963 2216 100-101 -2.3713799039462558 2216 102-103 -2.6224960582182497 2216 104-105 -2.5505268632607887 2216 106-107 -2.314977516403406 2216 108-109 -2.2240104403104155 2216 110-111 -2.2918507778826047 2216 112-113 -2.4937641195998204 2216 114-115 -2.3439395386581516 2216 116-117 -2.194586702692291 2216 118-119 -1.9346244098556404 2216 120-121 -1.90810811453332 2216 122-123 -1.9863727716814559 2216 124-125 -1.9336992538468074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30731.0 3 5903.0 4 3854.0 5 3439.0 6 3652.0 7 4318.0 8 4639.0 9 4856.0 10 5429.0 11 6216.0 12 6914.0 13 7471.0 14 7350.0 15 7029.0 16 6418.0 17 5826.0 18 5547.0 19 5300.0 20 5287.0 21 5496.0 22 6695.0 23 9240.0 24 14030.0 25 20036.0 26 22963.0 27 23852.0 28 28256.0 29 33654.0 30 40445.0 31 49225.0 32 62401.0 33 80946.0 34 110875.0 35 161800.0 36 282635.0 37 494616.0 38 214585.0 39 570.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.363776062039086 19.661606000655546 12.598562375514485 23.37605556179088 2 15.088881847171423 21.171252089270133 40.16921693374364 23.570649129814804 3 18.07504495121057 27.402414171500233 29.5068504919668 25.015690385322394 4 11.746751518728804 17.115316273285828 38.69239528177308 32.44553692621229 5 12.407132285132965 40.48242954271455 34.59652579765675 12.513912374495737 6 30.384726102596606 38.296542921155755 18.391449688032775 12.92728128821487 7 27.886542753998743 33.818652060614816 21.862717915044865 16.432087270341572 8 26.032496966484885 36.30789829704738 20.41088439169305 17.248720344774682 9 26.935938275806283 16.459450261926147 20.253000016736493 36.35161144553108 10-11 23.895906951857633 28.38104478582083 28.256485520578224 19.46656274174331 12-13 24.470384636949348 25.90484064745783 28.146680268112505 21.478094447480313 14-15 22.06811557292014 26.898233913622978 25.659036062908612 25.374614450548272 16-17 21.792093039344003 30.238791015182603 26.42506385129594 21.544052094177456 18-19 21.8211116107945 29.124182729379783 27.7738100684839 21.280895591341814 20-21 22.915832605404187 28.229431735404475 27.572021742181118 21.282713917010216 22-23 22.586685279445547 28.302219975595833 27.34996097230568 21.76113377265294 24-25 22.171768725037307 28.515351368451537 27.561883491012523 21.750996415498637 26-27 21.996039745378727 28.69803700143223 27.319591655233555 21.986331597955484 28-29 21.900095489615122 28.39974971287559 27.642360565773878 22.057794231735414 30-31 22.037332714348405 28.31677483558445 27.64421460661211 22.00167784345503 32-33 21.971448002146783 28.46831346386982 27.394056957826972 22.166181576156422 34-35 22.375211161370803 28.325917115396205 27.34857749203891 21.950294231194086 36-37 22.477190255680572 28.0609018569415 27.275709411012222 22.186198476365703 38-39 22.100833280566988 28.289112345456203 27.443000490657287 22.16705388331952 40-41 22.277728993558625 28.04852399532713 27.66678903013377 22.006957980980477 42-43 22.018097058433 27.96826236327343 27.523562773997096 22.490077804296476 44-45 21.868537225303307 28.26033472117076 27.55589053583393 22.315237517691997 46-47 22.258423186420025 28.318938800968276 27.207284055514673 22.215353957097022 48-49 22.037370710180745 28.290789775039833 27.281801243570204 22.39003827120922 50-51 22.193545651538383 28.10429456751938 27.36994561493949 22.332214166002753 52-53 22.305325410773566 28.030630178257592 27.409574022930787 22.25447038803805 54-55 21.89897535895912 28.146719223805817 27.547919334276237 22.40638608295882 56-57 21.974178023283354 28.366485606149155 27.440529975135448 22.218806395432043 58-59 22.043654468886622 27.7757444424855 27.651438225166096 22.52916286346178 60-61 21.962495979360767 28.206991574302386 27.578642029621474 22.25187041671537 62-63 22.00053340713248 28.061985702903385 27.678445888189827 22.259035001774304 64-65 21.815754331669876 27.937070379331285 27.65456440882271 22.592610880176125 66-67 21.76540037115109 28.286573056841018 27.352836806225877 22.595189765782013 68-69 21.947972493999906 27.981125543202772 27.62897308347796 22.441928879319363 70-71 21.93505518256385 27.925998123201445 27.589440832307716 22.54950586192699 72-73 21.893929566286 28.026408109348434 27.390476509308815 22.68918581505675 74-75 21.85216223967255 28.263557448631953 27.3073077485323 22.576972563163196 76-77 21.987946345756605 27.939622936254555 27.621837171059514 22.450593546929333 78-79 22.121052606619337 27.95302531090827 27.476477366971487 22.449444715500906 80-81 22.011618334125167 28.311759469293822 27.182259652443143 22.494362544137868 82-83 22.17720921460687 28.107859734248112 27.343770052192895 22.371160998952124 84-85 22.0737323589244 28.068936195854853 27.30861378835508 22.548717656865666 86-87 22.053381296208414 27.94975727024508 27.466334341301383 22.530527092245116 88-89 22.332811632532927 27.855642319699363 27.370544270637172 22.441001777130534 90-91 22.210836918402148 28.074066903368156 27.371801649471077 22.343294528758616 92-93 22.2609334606536 27.87859158787958 27.357287062849178 22.50318788861764 94-95 22.067482405215237 27.64913478767002 27.49249803402044 22.790884773094298 96-97 22.30345074614874 27.556341246093787 27.47212159320485 22.668086414552622 98-99 22.24123781165807 27.679315148664713 27.229859143299805 22.849587896377404 100-101 22.003955744756826 27.71389755234806 27.570118225996325 22.712028476898784 102-103 22.070315169859747 27.602016975513138 27.480831060864958 22.84683679376216 104-105 22.01524233663754 27.73473123100043 27.35340779401783 22.896618638344194 106-107 22.0926259070572 27.667570388029255 27.37288227790227 22.86692142701127 108-109 22.11819609527768 27.77675186686342 27.05321502193931 23.051837015919595 110-111 22.044098238966026 28.029421142617554 27.093768106117857 22.83271251229856 112-113 21.98712120810578 28.030439766415327 27.381171901436485 22.60126712404241 114-115 22.02114660343144 28.191798019249546 27.148054121913795 22.639001255405216 116-117 21.87548297285309 28.15517621360442 27.273452603799058 22.69588820974343 118-119 21.887094050831216 28.277030011416837 26.951569563890132 22.88430637386181 120-121 21.955163751995265 28.357954515334217 26.98107116719943 22.705810565471083 122-123 22.05549916379608 28.433974488107598 26.9432045387709 22.567321809325417 124-125 22.019558090604708 28.439085425098387 26.93248334626488 22.608873138032028 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1861.0 1 1412.0 2 821.5 3 598.5 4 445.5 5 353.0 6 315.5 7 316.0 8 350.0 9 354.5 10 295.0 11 241.5 12 245.5 13 253.5 14 286.5 15 341.0 16 382.5 17 451.5 18 564.5 19 713.5 20 932.0 21 1255.5 22 1778.0 23 2694.0 24 3848.5 25 5397.5 26 7621.5 27 10298.5 28 13053.5 29 16832.5 30 21538.0 31 26059.5 32 32564.0 33 38839.0 34 43670.5 35 48093.0 36 54236.0 37 60214.0 38 62731.5 39 65600.5 40 66134.0 41 65910.0 42 66335.0 43 67179.5 44 69192.5 45 70854.0 46 69761.5 47 66934.0 48 64897.5 49 62711.5 50 60667.5 51 57489.0 52 54900.0 53 53778.5 54 52594.5 55 49277.5 56 44338.5 57 40022.5 58 36736.0 59 32381.5 60 26379.0 61 21311.0 62 17220.5 63 12998.5 64 9480.0 65 7211.0 66 5309.0 67 4107.0 68 2991.5 69 1825.0 70 1282.5 71 899.5 72 658.0 73 475.5 74 377.0 75 309.5 76 209.0 77 128.0 78 83.5 79 63.0 80 40.5 81 27.0 82 22.0 83 13.0 84 9.5 85 8.0 86 7.5 87 5.0 88 4.0 89 5.0 90 3.0 91 2.5 92 3.0 93 2.0 94 1.0 95 0.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09082292375058508 2 3.905162569128351E-4 3 0.0 4 0.002566249688284345 5 0.0016736411010550075 6 1.6736411010550076E-4 7 0.0 8 0.001338912880844006 9 5.020923303165022E-4 10-11 0.010013952587979129 12-13 0.0023709915598279273 14-15 0.004993029284814106 16-17 0.00295676594519718 18-19 0.014114373285563898 20-21 0.01754533754272666 22-23 0.010153422679733713 24-25 0.011129713322015799 26-27 0.010599726973348381 28-29 0.00878661578053879 30-31 0.017712701652832164 32-33 0.0027894018350916793 34-35 0.0017294291377568413 36-37 4.7419831196558553E-4 38-39 3.068342018600847E-4 40-41 0.0013947009175458397 42-43 0.00295676594519718 44-45 0.0012273368074403389 46-47 0.002008369321266009 48-49 0.008898191853942456 50-51 0.005299863486674191 52-53 0.008312417468573204 54-55 0.009149238019100708 56-57 0.010516044918295631 58-59 0.00842399354197687 60-61 0.010292892771488297 62-63 0.013779645065352897 64-65 0.008228735413520453 66-67 0.01322176469833456 68-69 0.01313808264328181 70-71 0.004741983119655855 72-73 0.013751751047001978 74-75 0.01322176469833456 76-77 0.005383545541726941 78-79 0.007224550752887449 80-81 0.013695963010300146 82-83 0.01679219904725191 84-85 0.012998612551527226 86-87 0.015592756258162487 88-89 0.015843802423320737 90-91 0.01269177834966714 92-93 0.009400284184258959 94-95 0.007559278973098451 96-97 0.00903766194569704 98-99 0.006136684037201694 100-101 0.009428178202609876 102-103 0.011799169762437802 104-105 0.010516044918295631 106-107 0.0053277575050251075 108-109 0.0030125539818990138 110-111 0.006387730202359946 112-113 0.009623436331066293 114-115 0.013891221138756562 116-117 0.011938639854192386 118-119 0.21829858761427484 120-121 0.02479778231396503 122-123 0.02633195332326545 124-125 0.23634601748731798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1792499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.00418252627998 #Duplication Level Percentage of deduplicated Percentage of total 1 84.65449574347933 49.949693185184465 2 8.474205084504966 10.000270871425219 3 2.7576264167905307 4.8813447730679975 4 1.2373177762586294 2.920276956535001 5 0.6678209102108092 1.9702113440472513 6 0.393933586707791 1.3946237552003182 7 0.27550431049343305 1.1379134636192005 8 0.21202847021304194 1.0008453245774598 9 0.1486312442678338 0.7892878559298634 >10 0.9613547181557854 11.334000523110467 >50 0.1395205893299392 5.6499902744330965 >100 0.07552601738320672 8.217297609360628 >500 0.0020351322047584336 0.7542440635090559 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.7894018350916792E-5 0.0 14-15 0.0 0.0 0.0 5.5788036701833584E-5 5.5788036701833584E-5 16-17 0.0 0.0 0.0 5.5788036701833584E-5 5.5788036701833584E-5 18-19 0.0 0.0 0.0 5.5788036701833584E-5 5.5788036701833584E-5 20-21 0.0 0.0 0.0 8.368205505275038E-5 5.5788036701833584E-5 22-23 0.0 0.0 0.0 3.347282202110015E-4 5.5788036701833584E-5 24-25 0.0 0.0 0.0 0.0010878667156857549 5.5788036701833584E-5 26-27 0.0 0.0 0.0 0.0017852171744586747 5.5788036701833584E-5 28-29 0.0 0.0 0.0 0.004602513027901271 5.5788036701833584E-5 30-31 0.0 0.0 0.0 0.01531381607465332 5.5788036701833584E-5 32-33 0.0 0.0 0.0 0.035648555452471656 5.5788036701833584E-5 34-35 0.0 0.0 0.0 0.06686196198714756 5.5788036701833584E-5 36-37 0.0 0.0 0.0 0.11765696940416703 5.5788036701833584E-5 38-39 0.0 0.0 0.0 0.1954812806032249 5.5788036701833584E-5 40-41 0.0 0.0 0.0 0.2820364195461197 5.5788036701833584E-5 42-43 0.0 0.0 0.0 0.3719109466727736 5.5788036701833584E-5 44-45 0.0 0.0 0.0 0.4742262059839364 5.5788036701833584E-5 46-47 0.0 0.0 0.0 0.5974898730766377 5.5788036701833584E-5 48-49 0.0 0.0 0.0 0.7352026416751138 5.5788036701833584E-5 50-51 0.0 0.0 0.0 0.89238543508253 5.5788036701833584E-5 52-53 0.0 0.0 0.0 1.0446309872418338 5.5788036701833584E-5 54-55 0.0 0.0 0.0 1.2000285634747914 5.5788036701833584E-5 56-57 0.0 0.0 0.0 1.380921272480487 5.5788036701833584E-5 58-59 0.0 0.0 0.0 1.590293774222468 5.5788036701833584E-5 60-61 0.0 0.0 0.0 1.8225393710122013 5.5788036701833584E-5 62-63 0.0 0.0 0.0 2.055482318260707 5.5788036701833584E-5 64-65 0.0 0.0 0.0 2.2851058773254547 5.5788036701833584E-5 66-67 0.0 0.0 0.0 2.5392761725390085 5.5788036701833584E-5 68-69 5.5788036701833584E-5 0.0 0.0 2.8366264081597814 5.5788036701833584E-5 70-71 5.5788036701833584E-5 0.0 0.0 3.1654968845170903 5.5788036701833584E-5 72-73 5.5788036701833584E-5 0.0 0.0 3.4949810292781196 5.5788036701833584E-5 74-75 5.5788036701833584E-5 0.0 0.0 3.8175474574881214 5.5788036701833584E-5 76-77 5.5788036701833584E-5 0.0 0.0 4.161062293479661 5.5788036701833584E-5 78-79 5.5788036701833584E-5 0.0 0.0 4.549347028924423 5.5788036701833584E-5 80-81 5.5788036701833584E-5 0.0 0.0 4.98521895967585 5.5788036701833584E-5 82-83 5.5788036701833584E-5 0.0 0.0 5.439640412630634 5.5788036701833584E-5 84-85 5.5788036701833584E-5 0.0 0.0 5.871886120996441 5.5788036701833584E-5 86-87 5.5788036701833584E-5 0.0 0.0 6.3207845583177455 5.5788036701833584E-5 88-89 5.5788036701833584E-5 0.0 0.0 6.815122351532692 5.5788036701833584E-5 90-91 5.5788036701833584E-5 0.0 0.0 7.361036184678484 5.5788036701833584E-5 92-93 5.5788036701833584E-5 0.0 0.0 7.935457704578916 5.5788036701833584E-5 94-95 5.5788036701833584E-5 0.0 0.0 8.504077268662353 5.5788036701833584E-5 96-97 5.5788036701833584E-5 0.0 0.0 9.070548993332771 5.5788036701833584E-5 98-99 5.5788036701833584E-5 0.0 0.0 9.661232725931786 5.5788036701833584E-5 100-101 5.5788036701833584E-5 0.0 0.0 10.334622222941267 5.5788036701833584E-5 102-103 5.5788036701833584E-5 0.0 0.0 11.054064744248114 5.5788036701833584E-5 104-105 5.5788036701833584E-5 5.5788036701833584E-5 0.0 11.767844779829723 5.5788036701833584E-5 106-107 5.5788036701833584E-5 5.5788036701833584E-5 0.0 12.463800537685097 5.5788036701833584E-5 108-109 5.5788036701833584E-5 5.5788036701833584E-5 0.0 13.174512231248105 5.5788036701833584E-5 110-111 5.5788036701833584E-5 1.1157607340366717E-4 0.0 13.928794381475248 5.5788036701833584E-5 112-113 5.5788036701833584E-5 1.1157607340366717E-4 0.0 14.740147693248364 5.5788036701833584E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1855 0.0 63.533916 1 GTATCAA 3535 0.0 49.84099 1 TCAACGC 4080 0.0 39.943626 4 GTACATG 11430 0.0 39.73403 1 ATCAACG 4140 0.0 39.21888 3 CAACGCA 4205 0.0 38.758404 5 TACATGG 11715 0.0 37.771435 2 AACGCAG 4485 0.0 36.338703 6 TATCAAC 4750 0.0 35.684868 2 ACATGGG 12060 0.0 35.260754 3 CATGGGA 9075 0.0 31.52503 4 CGCAGAG 5510 0.0 29.254927 8 CATGGGG 3195 0.0 29.04095 4 ACGCAGA 5565 0.0 28.965792 7 ATGGGAT 3865 0.0 27.547668 5 GAGTACA 8055 0.0 25.863474 1 AGTACAT 8265 0.0 24.466335 2 GCAGAGT 6540 0.0 24.193424 9 GGACCGA 325 2.3646862E-11 23.792568 6 ATGGGAG 3905 0.0 23.30514 5 >>END_MODULE