FastQCFastQC Report
Thu 26 May 2016
SRR1364418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792499
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43720.24390529646041645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38520.21489551737546297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24070.13428180434131343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.075.571141
GTATCAA61650.056.8162571
ATCAACG82050.042.4533123
TCAACGC82300.042.3243564
CAACGCA83400.041.882025
TATCAAC85100.040.7929842
AACGCAG87800.039.917586
GGACCGT1202.0760126E-839.6690836
GTACATG111250.035.4475561
TACATGG113200.034.4541362
TGGTATC14500.034.084472
CGCAGAG104650.033.656228
ACGCAGA105200.033.4283687
ACATGGG115150.032.8872453
CATGGGA88300.030.4123614
GCAGAGT117400.029.7476659
GTGGTAT20800.026.9211061
ATGGGAT37600.026.9039865
AGAGTAC113250.025.76817510-11
GAGTACT63450.025.17651212-13
Produced by FastQC (version 0.11.5)