##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364418_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1792499 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.960115458920757 33.0 31.0 34.0 30.0 34.0 2 31.88545488728306 33.0 31.0 34.0 30.0 34.0 3 32.0900089762951 33.0 31.0 34.0 30.0 34.0 4 35.78305259863464 37.0 35.0 37.0 35.0 37.0 5 35.740179492429284 37.0 35.0 37.0 33.0 37.0 6 35.80336558067815 37.0 35.0 37.0 33.0 37.0 7 35.72103415399395 37.0 35.0 37.0 33.0 37.0 8 35.735708639168 37.0 35.0 37.0 33.0 37.0 9 37.382796866274404 39.0 37.0 39.0 34.0 39.0 10-11 37.328777310336015 39.0 37.0 39.0 34.0 39.0 12-13 37.30283447856874 39.0 37.0 39.0 33.5 39.0 14-15 38.596552634060046 40.0 38.0 41.0 34.0 41.0 16-17 38.61783158595904 40.0 38.0 41.0 34.0 41.0 18-19 38.69188908891999 40.0 38.0 41.0 34.0 41.0 20-21 38.70671057557075 40.0 38.0 41.0 34.0 41.0 22-23 38.66739758292752 40.0 38.0 41.0 34.0 41.0 24-25 38.611199504155934 40.0 38.0 41.0 34.0 41.0 26-27 38.49537321917613 40.0 38.0 41.0 34.0 41.0 28-29 38.420819202688534 40.0 38.0 41.0 34.0 41.0 30-31 38.28960044050234 40.0 38.0 41.0 34.0 41.0 32-33 38.14432309306728 40.0 37.0 41.0 33.0 41.0 34-35 38.033665569687905 40.0 37.0 41.0 33.0 41.0 36-37 37.92870484167634 40.0 37.0 41.0 33.0 41.0 38-39 37.82960994678379 40.0 37.0 41.0 33.0 41.0 40-41 37.71075353459054 40.0 37.0 41.0 33.0 41.0 42-43 37.56188957427591 40.0 37.0 41.0 32.5 41.0 44-45 37.37217538196674 40.0 37.0 41.0 32.0 41.0 46-47 37.12384944147807 40.0 36.5 41.0 31.0 41.0 48-49 36.87874609692948 40.0 36.0 41.0 31.0 41.0 50-51 36.67535518848267 40.0 36.0 41.0 30.0 41.0 52-53 36.566347038408395 39.0 36.0 41.0 30.0 41.0 54-55 36.42744403204688 39.0 35.0 41.0 30.0 41.0 56-57 36.23594127528105 39.0 35.0 41.0 29.5 41.0 58-59 36.03317630860603 39.0 35.0 41.0 29.0 41.0 60-61 35.811814957776825 38.5 35.0 40.0 29.0 41.0 62-63 35.85890536061666 38.5 35.0 40.5 29.0 41.0 64-65 35.66063635181944 38.0 35.0 40.0 29.0 41.0 66-67 35.40170901071632 37.5 35.0 40.0 29.0 41.0 68-69 35.08716490218404 37.0 34.0 40.0 29.0 41.0 70-71 34.757585638820444 36.5 34.0 39.0 29.0 41.0 72-73 34.41004597492105 36.0 34.0 39.0 28.0 41.0 74-75 34.06100617071474 35.5 34.0 38.5 28.0 40.0 76-77 33.734072376051536 35.0 34.0 37.0 28.0 39.5 78-79 33.400926862441764 35.0 34.0 37.0 27.5 39.0 80-81 33.098584713296916 35.0 33.0 36.5 27.0 39.0 82-83 32.815181207911415 35.0 33.0 36.0 27.0 37.5 84-85 32.56830994047974 35.0 33.0 36.0 26.0 37.0 86-87 32.36860773701966 35.0 33.0 35.0 26.0 37.0 88-89 32.17727485482558 35.0 33.0 35.0 26.0 36.0 90-91 32.03637909979308 35.0 33.0 35.0 26.0 36.0 92-93 31.913998836261555 35.0 33.0 35.0 26.0 36.0 94-95 31.795448979329976 35.0 33.0 35.0 25.0 36.0 96-97 31.73221826065175 35.0 33.0 35.0 25.0 35.0 98-99 31.6586394190457 35.0 33.0 35.0 25.0 35.0 100-101 31.647546246887725 35.0 33.0 35.0 25.0 35.0 102-103 30.53521898756987 33.5 30.5 34.5 23.5 35.0 104-105 31.483868889187665 35.0 32.5 35.0 25.0 35.0 106-107 31.664954066919982 35.0 33.0 35.0 25.0 35.0 108-109 31.67049465578502 35.0 33.0 35.0 25.0 35.0 110-111 31.616573565731418 35.0 33.0 35.0 25.0 35.0 112-113 31.562157635792264 35.0 33.0 35.0 25.0 35.0 114-115 31.457253253697772 35.0 33.0 35.0 25.0 35.0 116-117 31.380358929070532 35.0 33.0 35.0 25.0 35.0 118-119 31.285539350370627 35.0 33.0 35.0 24.0 35.0 120-121 31.164005391355865 35.0 33.0 35.0 24.0 35.0 122-123 30.989196367752506 35.0 33.0 35.0 22.5 35.0 124-125 29.997308785109503 34.5 31.0 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3336049226836302 1101 2 0.4827201722351191 1101 3 0.362854875082796 1101 4 0.2559974965711973 1101 5 0.2723512837907123 1101 6 0.29922250388275273 1101 7 0.28431250917927997 1101 8 0.2959211717232648 1101 9 0.43626893151503054 1101 10-11 0.39774742072541613 1101 12-13 0.4043803861714892 1101 14-15 0.4927823327205658 1101 16-17 0.42962979405722024 1101 18-19 0.3594869822587441 1101 20-21 0.34995602002930326 1101 22-23 0.41510978462115844 1101 24-25 0.45819875665559806 1101 26-27 0.42204378141822474 1101 28-29 0.4150102387956096 1101 30-31 0.4227392595462618 1101 32-33 0.4372184724119563 1101 34-35 0.3299696394528979 1101 36-37 0.28228422916505025 1101 38-39 0.3513507128414801 1101 40-41 0.26598976783800765 1101 42-43 0.22057889242190498 1101 44-45 0.25023926958651543 1101 46-47 0.38635017650415904 1101 48-49 0.2183154837315513 1101 50-51 0.24197868143644286 1101 52-53 0.24274523400442405 1101 54-55 0.2701829477277684 1101 56-57 0.26495690925239046 1101 58-59 0.060420452505532296 1101 60-61 0.06610925502488385 1101 62-63 0.15574699997925734 1101 64-65 0.22737021400437385 1101 66-67 0.25955070514863365 1101 68-69 0.32691335040919256 1101 70-71 0.26399317358500696 1101 72-73 0.24162776844188727 1101 74-75 0.22360264778441064 1101 76-77 0.23590666502905577 1101 78-79 0.2786964484065635 1101 80-81 0.2336720153473948 1101 82-83 0.2573408218278175 1101 84-85 0.1273380885690898 1101 86-87 0.18354477014086967 1101 88-89 0.20156048572503948 1101 90-91 0.23479305937727446 1101 92-93 0.23165687854899986 1101 94-95 0.2173620183909648 1101 96-97 0.1362040607660795 1101 98-99 0.26002203670248747 1101 100-101 0.20146647831253617 1101 102-103 0.2912633589708733 1101 104-105 0.3136778711257975 1101 106-107 0.2850322298206933 1101 108-109 0.250839611279293 1101 110-111 0.2628825955876515 1101 112-113 0.2983708662075344 1101 114-115 0.3825434035565074 1101 116-117 0.340377962911802 1101 118-119 0.33808102339069634 1101 120-121 0.3490172770046236 1101 122-123 0.45093728792751264 1101 124-125 0.4938329993019188 1102 1 0.38659176874418577 1102 2 0.48034921524887864 1102 3 0.3218872813864593 1102 4 0.26768435887118613 1102 5 0.21864713858946772 1102 6 0.2773635381617723 1102 7 0.3770196223957569 1102 8 0.279767429225366 1102 9 0.32974240768625407 1102 10-11 0.38106883362505073 1102 12-13 0.3707706369843251 1102 14-15 0.49028111476321357 1102 16-17 0.4520139499004827 1102 18-19 0.39307980097485995 1102 20-21 0.42220766902263307 1102 22-23 0.3733350566316247 1102 24-25 0.3655735316358104 1102 26-27 0.4410155835459406 1102 28-29 0.40926148007554985 1102 30-31 0.46788176919490354 1102 32-33 0.4602031416976615 1102 34-35 0.402481464868643 1102 36-37 0.28819330908032015 1102 38-39 0.2553366294537298 1102 40-41 0.20365211896215385 1102 42-43 0.29126948288071475 1102 44-45 0.36077866057328833 1102 46-47 0.3861670510010029 1102 48-49 0.16159806020372258 1102 50-51 0.2890063472082218 1102 52-53 0.23076744401828364 1102 54-55 0.2457398392932788 1102 56-57 0.267770822644394 1102 58-59 0.160595285533077 1102 60-61 0.08846196856476496 1102 62-63 0.2372095162618777 1102 64-65 0.311345695918142 1102 66-67 0.2489121499743021 1102 68-69 0.19793391229352153 1102 70-71 0.24731562304410204 1102 72-73 0.25415700805642416 1102 74-75 0.3078303591647895 1102 76-77 0.2268702852474931 1102 78-79 0.19198514057950433 1102 80-81 0.193015389486618 1102 82-83 0.12872901679774174 1102 84-85 0.10807121251741592 1102 86-87 0.1504304963716976 1102 88-89 0.11922760502267238 1102 90-91 0.013931263848995457 1102 92-93 0.16911260882241308 1102 94-95 0.14220973053149777 1102 96-97 0.08703175348839665 1102 98-99 0.15570407554582744 1102 100-101 0.03356285069997256 1102 102-103 0.0752993068526635 1102 104-105 0.1902326239236416 1102 106-107 0.07404158995254306 1102 108-109 0.06852677072632574 1102 110-111 0.0036539340277670362 1102 112-113 0.016620531917112658 1102 114-115 -0.0027229403946442687 1102 116-117 -0.017899137071268 1102 118-119 -0.05920871916248771 1102 120-121 -0.1407366783334858 1102 122-123 -0.27311261895489736 1102 124-125 -0.501036031411342 1103 1 0.33570503458342316 1103 2 0.46404422202982687 1103 3 0.3231097719212386 1103 4 0.2694176488722704 1103 5 0.15718418149250368 1103 6 0.165847307080206 1103 7 0.23735566673726538 1103 8 0.24946543361069473 1103 9 0.3337699757734214 1103 10-11 0.3408045343253647 1103 12-13 0.3812920775348161 1103 14-15 0.3577405833397904 1103 16-17 0.43545431114883826 1103 18-19 0.3437855270025949 1103 20-21 0.3178966586544263 1103 22-23 0.3265559043804771 1103 24-25 0.3691553671954111 1103 26-27 0.32560802042277714 1103 28-29 0.40183253605231783 1103 30-31 0.37002369876903174 1103 32-33 0.376745433836561 1103 34-35 0.292804797502626 1103 36-37 0.3166841263457556 1103 38-39 0.3169967071500963 1103 40-41 0.23940986425903077 1103 42-43 0.19178830429836324 1103 44-45 0.16632566852256048 1103 46-47 0.07093363964219179 1103 48-49 0.057740324368644735 1103 50-51 0.0038311189939790324 1103 52-53 -0.04353111518440045 1103 54-55 -0.047412911001877944 1103 56-57 -0.034771793115105254 1103 58-59 -0.15879795326464574 1103 60-61 -0.21826200363128834 1103 62-63 0.012125148645331763 1103 64-65 0.05588370694043476 1103 66-67 -0.07895679465229222 1103 68-69 0.0014832486656004562 1103 70-71 -0.09004904937917502 1103 72-73 -0.0036637088729136735 1103 74-75 -0.008424582562930993 1103 76-77 -0.04596011973077907 1103 78-79 0.003660370850639083 1103 80-81 -0.016998405527154148 1103 82-83 0.04663120109967167 1103 84-85 0.05778111955876852 1103 86-87 0.0927293545120591 1103 88-89 0.18220300848568627 1103 90-91 0.10849793449235534 1103 92-93 0.1186378052916588 1103 94-95 0.16593009216380494 1103 96-97 0.1279422655357223 1103 98-99 0.1303370884124888 1103 100-101 0.16955638651558402 1103 102-103 0.19521205270490327 1103 104-105 0.2666620053831963 1103 106-107 0.1672725028506079 1103 108-109 0.14371700067522042 1103 110-111 0.0945622520371181 1103 112-113 0.10523985124425295 1103 114-115 0.15948338419407548 1103 116-117 0.1104809498400634 1103 118-119 0.039387987860767026 1103 120-121 0.09268949023170947 1103 122-123 0.14487842388506422 1103 124-125 0.008371660611548037 1104 1 0.25043546261196425 1104 2 0.38176263738584026 1104 3 0.2205327323699109 1104 4 0.18812220934814405 1104 5 0.16379140777178947 1104 6 0.13755069060511005 1104 7 0.18117211312257808 1104 8 0.21455403418872976 1104 9 0.1298199706861638 1104 10-11 0.1992512828937123 1104 12-13 0.12249895021602697 1104 14-15 0.20773459198191802 1104 16-17 0.3424363211522419 1104 18-19 0.2762120512296775 1104 20-21 0.23860656979071848 1104 22-23 0.2401164658084909 1104 24-25 0.2613964103178006 1104 26-27 0.18521734880505392 1104 28-29 0.17756811691851482 1104 30-31 0.16501414093394118 1104 32-33 0.0719985916465049 1104 34-35 0.11774595670082988 1104 36-37 0.1254886966183122 1104 38-39 0.15086681127287704 1104 40-41 0.09617323018228774 1104 42-43 0.057896366803880994 1104 44-45 0.047105945728112886 1104 46-47 0.15422451170052653 1104 48-49 0.1690885634087067 1104 50-51 0.04914744244850766 1104 52-53 0.09575102028667715 1104 54-55 0.09949700569395503 1104 56-57 0.16290644775745022 1104 58-59 0.0388954009430833 1104 60-61 0.03339911383252314 1104 62-63 0.18005326152682954 1104 64-65 0.2643211581088494 1104 66-67 0.25240996092217216 1104 68-69 0.2541489980967526 1104 70-71 0.29397303822041465 1104 72-73 0.21806551575631516 1104 74-75 0.23887451600759135 1104 76-77 0.2697292494143255 1104 78-79 0.2626501064671345 1104 80-81 0.23179468670710435 1104 82-83 0.2452710953926882 1104 84-85 0.15021522204391147 1104 86-87 0.1649141517383299 1104 88-89 0.20761938761665277 1104 90-91 0.10280945055688306 1104 92-93 0.19333195591817187 1104 94-95 0.17898133419657825 1104 96-97 0.2738752130651889 1104 98-99 0.1789130344060652 1104 100-101 0.04923244567025975 1104 102-103 0.10947875773065618 1104 104-105 0.20045959248526302 1104 106-107 0.1054822392389525 1104 108-109 0.11679785674849796 1104 110-111 0.2029714196782777 1104 112-113 0.15350314011649147 1104 114-115 0.23087378674045667 1104 116-117 0.0643149061036894 1104 118-119 -0.04375525929866697 1104 120-121 0.06296358405907299 1104 122-123 -0.07800636365247726 1104 124-125 -0.3186028493992801 1105 1 0.2833771782206149 1105 2 0.3776018957168574 1105 3 0.18764739502424277 1105 4 0.17063693073414044 1105 5 0.12171744802509465 1105 6 0.14233417932564407 1105 7 0.13631803261002062 1105 8 0.06719250456886527 1105 9 0.18697679678133738 1105 10-11 0.2116898664700244 1105 12-13 0.17813304597915902 1105 14-15 0.18147208418626803 1105 16-17 0.20668774389520905 1105 18-19 0.2718253439310061 1105 20-21 0.24129048544728704 1105 22-23 0.2684116944617898 1105 24-25 0.28928050436760344 1105 26-27 0.12635462767715921 1105 28-29 0.1729132896008636 1105 30-31 0.1713339373204832 1105 32-33 0.1674408487321557 1105 34-35 0.12307706455826661 1105 36-37 0.05779585936024745 1105 38-39 0.01346561385547318 1105 40-41 0.045047585145781 1105 42-43 0.013084376026647249 1105 44-45 0.13518249913352065 1105 46-47 0.0851114206549326 1105 48-49 -0.047523382291785765 1105 50-51 0.023026526849996287 1105 52-53 0.05148787707179281 1105 54-55 -0.045900530239748605 1105 56-57 -0.10348912049729364 1105 58-59 -0.06078893250744244 1105 60-61 0.009319324107927685 1105 62-63 0.09525867301250202 1105 64-65 0.1094258244833668 1105 66-67 0.034963138490290646 1105 68-69 0.014450089054932391 1105 70-71 0.002205247354808648 1105 72-73 0.010434976478840952 1105 74-75 0.05542281633874069 1105 76-77 0.08024016190555727 1105 78-79 0.03445010018276662 1105 80-81 0.10988451615897787 1105 82-83 0.06293493534462868 1105 84-85 0.08811068614110695 1105 86-87 0.07762307943023217 1105 88-89 0.146072362908356 1105 90-91 -0.022661731422452647 1105 92-93 -0.0033303603982801633 1105 94-95 -0.117188874882725 1105 96-97 0.05617274883079659 1105 98-99 0.07848931813975923 1105 100-101 0.04657206405924441 1105 102-103 0.052859852676693464 1105 104-105 0.10690490809168551 1105 106-107 0.08010277538330257 1105 108-109 0.025308737429984518 1105 110-111 0.07280623007468634 1105 112-113 0.07506442490469567 1105 114-115 0.025991558474952825 1105 116-117 0.05226036711689375 1105 118-119 -0.04501956770742055 1105 120-121 -0.026566843198938983 1105 122-123 -0.011572727139434136 1105 124-125 -0.09748044869905215 1106 1 0.2015935834798377 1106 2 0.3436122443787468 1106 3 0.1585335154739127 1106 4 0.13650652821962694 1106 5 0.153296491333748 1106 6 0.09556533676644108 1106 7 0.11589999770997395 1106 8 0.05840799058358925 1106 9 0.19034584089725826 1106 10-11 0.24622422876694117 1106 12-13 0.2526921731916616 1106 14-15 0.2730975844974566 1106 16-17 0.17147631766002291 1106 18-19 0.223467549492824 1106 20-21 0.2535125311699389 1106 22-23 0.21214351464402625 1106 24-25 0.14580232275302052 1106 26-27 0.315935264690161 1106 28-29 0.2958818373768395 1106 30-31 0.16182424879593071 1106 32-33 0.2715633868886158 1106 34-35 0.19696029967392548 1106 36-37 0.23650493279824758 1106 38-39 0.07990277320767802 1106 40-41 0.1641211273099401 1106 42-43 0.0931655455764755 1106 44-45 0.0705573972484217 1106 46-47 0.21582252007656422 1106 48-49 0.21330391171802177 1106 50-51 0.2909823926975932 1106 52-53 0.24428554432611804 1106 54-55 0.272812670684182 1106 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2216 118-119 -0.49755510430562566 2216 120-121 -0.6194046660410848 2216 122-123 -0.712672757104432 2216 124-125 -0.5912162937909677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73.0 3 15.0 4 30.0 5 149.0 6 374.0 7 842.0 8 1428.0 9 2353.0 10 3891.0 11 5043.0 12 6607.0 13 9151.0 14 13238.0 15 10411.0 16 5297.0 17 4650.0 18 4618.0 19 4610.0 20 4589.0 21 4697.0 22 5127.0 23 6012.0 24 7868.0 25 10488.0 26 14046.0 27 18469.0 28 23846.0 29 30886.0 30 38954.0 31 48313.0 32 61927.0 33 79247.0 34 104924.0 35 151265.0 36 274136.0 37 556673.0 38 277276.0 39 976.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.12972604628086 20.00056926027975 13.088744887121825 23.78095980631756 2 15.27399870229787 21.550447193956256 39.33660976155421 23.838944342191663 3 18.247136376112703 27.01217974609536 30.274077760247792 24.466606117544146 4 12.109209681471762 17.361603528656374 38.297590798693506 32.23159599117836 5 12.083568353287015 39.95791120402187 34.947927389016186 13.010593053674931 6 30.38272266818559 38.37954721313652 17.801850935481696 13.435879183196198 7 27.286188275608502 33.71545032507004 22.109895039174106 16.888466360147355 8 26.479680044451907 35.41513830691119 20.2422986010034 17.8628830476335 9 27.084980242666802 14.98734448387419 20.459202487700132 37.46847278575888 10-11 24.1807387340244 27.36757454257994 28.374743863176494 20.07694286021917 12-13 25.08422598841059 24.98492328308133 28.63775656220729 21.29309416630079 14-15 22.339398794643678 25.822385396030906 26.784282724843916 25.0539330844815 16-17 21.33022110472586 29.42102059750103 27.47496651323097 21.77379178454214 18-19 21.23644906320572 27.666393676750182 29.949243959261274 21.147913300782818 20-21 22.267711167481824 26.817895016956772 29.960546700444464 20.953847115116943 22-23 21.916302324296975 27.02230238343229 29.704702764129852 21.35669252814088 24-25 21.565005057185527 27.206375010529992 29.982108776629723 21.24651115565476 26-27 21.391575722209268 27.436783327735892 29.76829386673034 21.4033470833245 28-29 21.195746468454164 27.11693306562335 30.056257032826462 21.631063433096028 30-31 21.335330933126617 27.095436529576684 30.02530844757653 21.54392408972016 32-33 21.41872882495332 27.10983381301747 29.780462917970947 21.690974444058266 34-35 21.80851983738903 27.055189431068023 29.72712955488399 21.409161176658955 36-37 21.95917543050233 26.83359934928834 29.49323821101155 21.713987009197773 38-39 21.71807165196279 27.049095731208627 29.566281245502086 21.666551371326495 40-41 21.74224611967764 26.885278917652062 29.734526061333682 21.637948901336618 42-43 21.736428330741028 26.815246328558583 29.398784033776593 22.0495413069238 44-45 21.575214267901963 27.189080719152425 29.302471019509635 21.93323399343598 46-47 22.01792023314936 27.27016863049854 28.689137344009673 22.022773792342424 48-49 21.91220190359939 27.4439204708064 28.436305961676965 22.207571663917246 50-51 22.1391197428841 27.35664008738638 28.319987905153642 22.18425226457588 52-53 22.139091848865746 27.366598251937656 28.399485857453755 22.094824041742843 54-55 21.735604873419735 27.490531375470784 28.48327948857991 22.290584262529574 56-57 21.78450337868465 27.63796121311864 28.43381055655897 22.14372485163774 58-59 21.81373396783246 27.078756366841656 28.709309396426203 22.398200268899686 60-61 21.77856662281715 27.454764144259343 28.620737363695497 22.145931869228015 62-63 21.754743891107868 27.270703677762132 28.877017131760045 22.097535299369955 64-65 21.535688443898714 27.092957931915162 28.854297826665455 22.51705579752067 66-67 21.51592358819926 27.43394971709871 28.50979471106802 22.54033198363401 68-69 21.745758296099467 27.150419609718053 28.66065755127339 22.443164542909088 70-71 21.679286850369234 27.046988589672853 28.620015966536105 22.653708593421808 72-73 21.6387847357237 27.24804867394626 28.283251482985484 22.82991510734455 74-75 21.51340112323633 27.415217525923307 28.22406037604484 22.847320974795522 76-77 21.62015153146529 27.087155976098177 28.437868026704617 22.85482446573192 78-79 21.640709422989914 27.129638566046623 28.372121825451508 22.85753018551196 80-81 21.534237954944466 27.510503492610038 28.002749234448665 22.95250931799683 82-83 21.70137891290316 27.20673763276855 28.17150804547171 22.920375408856575 84-85 21.49884044565715 27.204757157465636 28.10562237412685 23.190780022750364 86-87 21.37125320572006 27.058369349160028 28.31650115285978 23.253876292260134 88-89 21.589300747169176 27.051451632609 28.142498266386763 23.216749353835063 90-91 21.463863388296716 27.201949906178342 28.206889106896444 23.127297598628505 92-93 21.423251625946865 26.969630023866188 28.171251898193244 23.435866451993704 94-95 21.22745454646888 26.631585113445546 28.35653197507378 23.7844283650118 96-97 21.43767118835929 26.51512594470032 28.301663632151485 23.745539234788907 98-99 21.23162785196162 26.62416679173741 28.130622952722145 24.013582403578827 100-101 21.018081867383643 26.5997713924776 28.40285254424214 23.979294195896614 102-103 21.098754256047737 26.37244549957387 28.310734376591007 24.218065867787388 104-105 20.982003629735715 26.407498924636396 28.231851181951523 24.378646263676366 106-107 20.989163199234493 26.309196843671927 28.336511328494968 24.365128628598608 108-109 20.93863631671715 26.426964110455636 27.979566455330705 24.65483311749651 110-111 20.780335178574816 26.60115892026035 28.05092509420551 24.567580806959324 112-113 20.625752307308208 26.577512584366513 28.354122469701977 24.442612638623302 114-115 20.68604035524938 26.59742550759276 28.09728798486525 24.61924615229261 116-117 20.34403376069771 26.575642880593765 28.242283047935608 24.838040310772925 118-119 20.2589542192684 26.666114358940884 28.03031444161968 25.044616980171035 120-121 20.13973830309566 26.75119881885206 28.079376577934596 25.02968630011769 122-123 20.176904505876596 26.831660383516247 28.04170728747819 24.949727823128967 124-125 19.974105697862605 26.715748646687874 28.106590769574197 25.203554885875327 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 298.0 1 212.0 2 260.0 3 366.0 4 229.5 5 190.0 6 408.5 7 963.5 8 1652.5 9 1897.0 10 1466.0 11 951.0 12 857.0 13 949.5 14 1048.5 15 1175.0 16 1328.5 17 1457.5 18 1640.5 19 1811.5 20 2020.0 21 2410.5 22 2979.5 23 3794.5 24 4916.5 25 6522.5 26 8671.5 27 11126.0 28 13652.0 29 17291.0 30 22000.5 31 26290.5 32 32451.0 33 38595.0 34 43308.5 35 47143.5 36 53126.5 37 59258.0 38 61685.5 39 64793.5 40 65308.5 41 64785.5 42 65020.5 43 66264.0 44 68625.5 45 70397.5 46 69060.5 47 66400.0 48 64695.0 49 62127.0 50 60231.0 51 56953.5 52 54248.0 53 53649.5 54 52359.0 55 48653.5 56 44059.0 57 39751.5 58 36154.5 59 32077.5 60 26323.5 61 21010.5 62 16813.0 63 12717.5 64 9263.0 65 7037.5 66 5021.0 67 3873.5 68 2852.0 69 1667.0 70 1119.5 71 765.5 72 575.0 73 458.0 74 382.0 75 296.5 76 182.5 77 101.0 78 72.0 79 51.0 80 27.0 81 11.0 82 5.0 83 2.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03905162569128351 2 0.005578803670183359 3 8.368205505275037E-4 4 0.08083686518095687 5 0.0054672275967796915 6 0.0 7 0.006471412257412696 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.6736411010550076E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.252444771238366E-4 28-29 6.69456440422003E-4 30-31 8.08926532176587E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.5788036701833584E-5 40-41 0.0014225949358967565 42-43 8.368205505275037E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.415624220710862E-4 58-59 5.020923303165022E-4 60-61 2.789401835091679E-4 62-63 5.29986348667419E-4 64-65 0.0 66-67 5.5788036701833584E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.3947009175458396E-4 92-93 2.789401835091679E-4 94-95 5.020923303165022E-4 96-97 0.0036541164039701 98-99 0.010041846606330045 100-101 0.019218978643781668 102-103 0.0109623492119103 104-105 0.004574619009550354 106-107 0.012635990312965308 108-109 0.013947009175458396 110-111 0.007866113174958536 112-113 0.005383545541726941 114-115 0.0037935864957246842 116-117 2.7894018350916792E-5 118-119 0.0011157607340366717 120-121 3.068342018600847E-4 122-123 3.626222385619183E-4 124-125 0.03341703398439832 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1792499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.21379156788031 #Duplication Level Percentage of deduplicated Percentage of total 1 79.92850713681221 40.93441904836534 2 11.039183112145153 11.307168459701323 3 3.593222584713234 5.520676575315112 4 1.6007147319361643 3.279146825640565 5 0.9081674784926022 2.325534997612378 6 0.554141619645438 1.702781604456544 7 0.36650043060483933 1.3138913663774239 8 0.26402450853925186 1.0817356919313024 9 0.2040470535108293 0.940502094168936 >10 1.254795561676055 12.643139777004752 >50 0.17857651734969873 6.365294840934041 >100 0.10273892202473389 9.655324089869852 >500 0.004099308609403321 1.255447997432506 >1k 0.0012810339404385378 1.6749366311900018 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4372 0.24390529646041645 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3852 0.21489551737546297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2407 0.13428180434131343 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.7894018350916792E-5 0.0 22-23 0.0 0.0 0.0 2.789401835091679E-4 0.0 24-25 0.0 0.0 0.0 0.0010320786789839212 0.0 26-27 0.0 0.0 0.0 0.0017852171744586747 0.0 28-29 0.0 0.0 0.0 0.004658301064603104 0.0 30-31 0.0 0.0 0.0 0.01581590840496982 0.0 32-33 0.0 0.0 0.0 0.03668063413145558 0.0 34-35 0.0 0.0 0.0 0.06853560308820256 0.0 36-37 0.0 0.0 0.0 0.12016743105574954 0.0 38-39 0.0 0.0 0.0 0.19969327737421333 0.0 40-41 0.0 0.0 0.0 0.28655525051896824 0.0 42-43 0.0 0.0 0.0 0.37737817426955333 0.0 44-45 0.0 0.0 0.0 0.4807534062780509 0.0 46-47 0.0 0.0 0.0 0.6055233503617017 0.0 48-49 0.0 0.0 0.0 0.7444355617492674 0.0 50-51 0.0 0.0 0.0 0.9019809773952454 0.0 52-53 0.0 0.0 0.0 1.0549238800133223 0.0 54-55 0.0 0.0 0.0 1.2103214562462796 0.0 56-57 0.0 0.0 0.0 1.391911515710748 0.0 58-59 0.0 0.0 0.0 1.6026787183702753 0.0 60-61 0.0 0.0 0.0 1.8370442605546782 0.0 62-63 0.0 0.0 0.0 2.072107153197854 0.0 64-65 0.0 0.0 0.0 2.303320671308603 0.0 66-67 0.0 0.0 0.0 2.559164607623212 0.0 68-69 0.0 0.0 0.0 2.857909544161531 0.0 70-71 0.0 0.0 0.0 3.1875889470510166 0.0 72-73 0.0 0.0 0.0 3.5194440833718734 0.0 74-75 0.0 0.0 0.0 3.8443536091233526 0.0 76-77 0.0 0.0 0.0 4.1904346948031765 0.0 78-79 0.0 0.0 0.0 4.58273616889047 0.0 80-81 0.0 0.0 0.0 5.023684810981763 0.0 82-83 0.0 0.0 0.0 5.4863071053317185 0.0 84-85 0.0 0.0 0.0 5.925944728560518 0.0 86-87 0.0 0.0 0.0 6.379473572928074 0.0 88-89 0.0 0.0 0.0 6.877995468895659 0.0 90-91 0.0 0.0 0.0 7.431412792977849 0.0 92-93 0.0 0.0 0.0 8.01317043970457 0.0 94-95 0.0 0.0 0.0 8.587675641660052 0.0 96-97 0.0 0.0 0.0 9.158749879358371 0.0 98-99 0.0 0.0 0.0 9.757550771297502 0.0 100-101 0.0 0.0 0.0 10.437300104490994 0.0 102-103 0.0 0.0 0.0 11.162935097871742 0.0 104-105 0.0 0.0 0.0 11.883298121784168 0.0 106-107 0.0 0.0 0.0 12.585725291896956 0.0 108-109 0.0 0.0 0.0 13.306060421791031 0.0 110-111 0.0 0.0 0.0 14.071946483652152 0.0 112-113 0.0 0.0 0.0 14.892644291572825 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3090 0.0 75.57114 1 GTATCAA 6165 0.0 56.816257 1 ATCAACG 8205 0.0 42.453312 3 TCAACGC 8230 0.0 42.324356 4 CAACGCA 8340 0.0 41.88202 5 TATCAAC 8510 0.0 40.792984 2 AACGCAG 8780 0.0 39.91758 6 GGACCGT 120 2.0760126E-8 39.669083 6 GTACATG 11125 0.0 35.447556 1 TACATGG 11320 0.0 34.454136 2 TGGTATC 1450 0.0 34.08447 2 CGCAGAG 10465 0.0 33.65622 8 ACGCAGA 10520 0.0 33.428368 7 ACATGGG 11515 0.0 32.887245 3 CATGGGA 8830 0.0 30.412361 4 GCAGAGT 11740 0.0 29.747665 9 GTGGTAT 2080 0.0 26.921106 1 ATGGGAT 3760 0.0 26.903986 5 AGAGTAC 11325 0.0 25.768175 10-11 GAGTACT 6345 0.0 25.176512 12-13 >>END_MODULE