FastQCFastQC Report
Thu 26 May 2016
SRR1364417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441507
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.041.7537961
GTATCAA27750.041.404081
CAACGCA27450.039.8720325
TCAACGC27950.039.1539424
ATCAACG27750.039.006683
AACGCAG30650.036.096616
GTACATG78250.034.9963151
TACATGG79000.034.0284352
ACATGGG78750.032.3994263
TATCAAC35500.031.8314082
CATGGGG50850.027.3693434
CGCAGAG40300.027.0103368
ACGCAGA40950.026.872117
TAAGGTG12900.024.4387515
CATGGGA37900.023.539194
GAGTACA57200.022.3764531
GTAAGGT12550.021.3259434
TAGCCCT17200.019.7099674
ATGGGGA33450.019.38315
ATACCGA1550.00570003919.1876726
Produced by FastQC (version 0.11.5)