FastQCFastQC Report
Thu 26 May 2016
SRR1364417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441507
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.061.9014661
GTATCAA49250.052.0238271
ATCAACG59050.042.9563143
TCAACGC60050.042.2401054
CAACGCA60850.041.7535255
TATCAAC65000.039.0265582
AACGCAG64950.039.0230066
ACGCAGA78200.032.4110537
CGCAGAG78650.032.1493078
GTACATG75950.031.1460361
TACATGG78150.030.0976182
ACATGGG77700.029.2738363
TATACCG1505.296737E-627.7672335
GCAGAGT96300.025.886259
AGAGTAC83250.024.15537810-11
CAGAGTA87650.023.55368810-11
GAGTACT55800.023.29692612-13
CATGGGG49950.023.0064834
GTACTTT57050.022.36933314-15
TAAGGTA10700.021.7024734
Produced by FastQC (version 0.11.5)