FastQCFastQC Report
Thu 26 May 2016
SRR1364416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701055
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23350.046.400811
GTATCAA34700.044.090471
GTACATG105950.041.803541
ATCAACG36300.039.8157273
CAACGCA36400.039.7092825
TCAACGC36550.039.7068564
TACATGG116850.036.5978162
AACGCAG39650.036.3037346
ACATGGG113300.036.169653
TATCAAC44700.033.5311162
CATGGGG63950.029.762714
CATGGGA62500.029.4063744
ACGCAGA50650.028.4194097
CGCAGAG53250.026.9200958
GAGTACA80900.023.8417191
AGTACAT79900.023.0020582
ATGGGGA38050.022.5110115
ATGGGAG33200.021.8579245
GCAGAGT67600.020.7655989
ATGGGAT32400.020.745345
Produced by FastQC (version 0.11.5)