FastQCFastQC Report
Thu 26 May 2016
SRR1364416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701055
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32450.059.3073731
GTATCAA53900.050.297161
ATCAACG66700.039.817353
TCAACGC67150.039.5505144
CAACGCA68600.038.6835
TATCAAC72800.036.8081862
AACGCAG72150.036.6965336
GTACATG103250.036.586431
ACATGGG108900.033.5193753
TACATGG113000.033.0936362
ACGCAGA85500.030.8275477
CGCAGAG86850.030.4157458
CATGGGG62450.027.1753864
CATGGGA63200.027.1355554
GCAGAGT102150.025.5688619
GAGTACA82750.024.121131
AGTACAT80950.023.3187732
ATGGGAG31950.023.2781545
GTGGTAT20250.023.2446461
AGAGTAC98800.022.43130710-11
Produced by FastQC (version 0.11.5)