FastQCFastQC Report
Thu 26 May 2016
SRR1364415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419701
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.053.3573461
GTATCAA36950.048.663591
GTACATG122050.041.0270081
TCAACGC43550.039.7395174
ATCAACG43850.039.3320123
CAACGCA44100.039.1130835
TACATGG126600.039.0847552
AACGCAG45750.037.7024466
ACATGGG128800.036.9857443
TATCAAC48500.036.6646232
CATGGGA84050.033.468884
ACGCAGA56200.030.480277
CGCAGAG57350.029.9727828
CATGGGG55600.029.7363574
ATGGGAG38900.027.8281565
AGTACAT88100.025.3822252
GAGTACA94450.025.02651
GCAGAGT69150.023.8264439
ATGGGGA32950.022.9250835
GTATAGG7650.022.5708641
Produced by FastQC (version 0.11.5)