##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364415_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2419701 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.60292036082144 31.0 31.0 34.0 27.0 34.0 2 30.65362042665602 31.0 31.0 34.0 27.0 34.0 3 30.60715848776357 31.0 31.0 34.0 26.0 34.0 4 34.18204150016882 37.0 35.0 37.0 30.0 37.0 5 34.34891625039623 37.0 35.0 37.0 30.0 37.0 6 34.45106730129053 37.0 35.0 37.0 31.0 37.0 7 34.32689328144262 37.0 35.0 37.0 30.0 37.0 8 34.18535306634993 37.0 35.0 37.0 30.0 37.0 9 35.27906671113497 39.0 35.0 39.0 28.0 39.0 10-11 35.490642025605645 39.0 35.0 39.0 29.0 39.0 12-13 35.54646937782809 39.0 35.0 39.0 30.0 39.0 14-15 36.677245866328114 40.0 36.0 41.0 29.5 41.0 16-17 36.82147112391159 40.0 36.0 41.0 30.0 41.0 18-19 36.80098326198154 40.0 36.5 41.0 30.0 41.0 20-21 36.75254545912904 40.0 36.5 41.0 29.5 41.0 22-23 36.65519210844646 40.0 36.0 41.0 29.0 41.0 24-25 36.712901924659285 40.0 36.0 41.0 29.5 41.0 26-27 36.71518671108538 40.0 36.0 41.0 30.0 41.0 28-29 36.66461248724532 40.0 36.0 41.0 30.0 41.0 30-31 36.44944313367643 40.0 36.0 41.0 29.0 41.0 32-33 36.52182046459459 40.0 36.0 41.0 30.0 41.0 34-35 36.46491529325318 40.0 36.0 41.0 30.0 41.0 36-37 36.346993905445345 40.0 36.0 41.0 29.0 41.0 38-39 36.20630937458802 40.0 35.0 41.0 28.5 41.0 40-41 36.17820114964617 40.0 35.0 41.0 28.5 41.0 42-43 36.04322166251119 39.5 35.0 41.0 28.0 41.0 44-45 36.030109711902426 39.0 35.0 41.0 28.0 41.0 46-47 35.886350834255964 39.0 35.0 41.0 28.0 41.0 48-49 35.7243773094279 39.0 35.0 41.0 27.5 41.0 50-51 35.58914758476357 39.0 34.5 41.0 27.5 41.0 52-53 35.46422181914212 39.0 34.0 40.5 27.0 41.0 54-55 35.333880508376865 38.5 34.0 40.0 27.0 41.0 56-57 35.2866546321219 38.5 34.0 40.0 27.5 41.0 58-59 35.26648519796454 38.0 34.0 40.0 28.0 41.0 60-61 35.07898517213491 38.0 34.0 40.0 28.0 41.0 62-63 34.82467771844538 37.5 34.0 40.0 27.0 41.0 64-65 34.52044054203391 37.0 34.0 40.0 26.5 41.0 66-67 34.21084257931042 36.5 33.5 40.0 26.0 41.0 68-69 33.91216807365869 36.0 33.0 39.0 26.0 41.0 70-71 33.56828302339835 36.0 33.0 39.0 26.0 41.0 72-73 33.231424874395636 35.0 33.0 38.5 26.0 40.0 74-75 32.873031006723565 35.0 33.0 37.0 26.0 39.5 76-77 32.52461585129733 35.0 32.0 37.0 25.0 39.0 78-79 32.21293023394213 35.0 32.0 36.5 25.0 39.0 80-81 31.890256895376744 35.0 32.0 36.0 24.0 37.5 82-83 31.61232317546672 35.0 32.0 35.5 24.0 37.0 84-85 31.27880159573435 35.0 32.0 35.0 22.0 37.0 86-87 31.08880229416775 35.0 31.5 35.0 22.5 36.0 88-89 30.954135035692424 35.0 31.0 35.0 22.0 36.0 90-91 30.830139550299812 35.0 31.0 35.0 20.5 36.0 92-93 30.613751864383246 34.5 31.0 35.0 20.0 35.5 94-95 30.537150871120026 34.0 31.0 35.0 20.0 35.0 96-97 30.461744446937864 34.0 31.0 35.0 19.5 35.0 98-99 30.312107157041304 34.0 31.0 35.0 18.5 35.0 100-101 30.18299802330949 34.0 31.0 35.0 17.5 35.0 102-103 29.151178802670245 33.5 29.0 34.5 15.0 35.0 104-105 30.096834278284796 34.0 30.5 35.0 18.0 35.0 106-107 30.34157753375314 35.0 31.0 35.0 18.0 35.0 108-109 30.322505135965145 35.0 32.0 35.0 17.0 35.0 110-111 30.21541421853361 35.0 31.0 35.0 13.0 35.0 112-113 30.096868373406465 35.0 31.0 35.0 8.5 35.0 114-115 29.990859201198823 35.0 31.0 35.0 3.5 35.0 116-117 29.864580169202725 35.0 31.0 35.0 2.0 35.0 118-119 29.59421308665823 34.5 31.0 35.0 2.0 35.0 120-121 29.456430773884875 34.0 31.0 35.0 2.0 35.0 122-123 29.204437035815584 34.0 31.0 35.0 2.0 35.0 124-125 28.014610069591242 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8126857120005795 1101 2 0.8162730273370755 1101 3 1.0105854081398462 1101 4 0.7320418676157914 1101 5 0.7277149829152734 1101 6 0.8102881009246872 1101 7 0.8348597615785778 1101 8 0.8362345638695174 1101 9 1.3975422533217525 1101 10-11 1.2042505704916309 1101 12-13 1.0718298703312428 1101 14-15 1.279976575011979 1101 16-17 1.086951515094313 1101 18-19 1.1690195100419274 1101 20-21 1.1976587080842265 1101 22-23 1.192283651284086 1101 24-25 1.1669756588665905 1101 26-27 1.0961154863729448 1101 28-29 1.0933181341574425 1101 30-31 1.3036686287451076 1101 32-33 1.1289123709073579 1101 34-35 1.1273234878427516 1101 36-37 1.045159517891591 1101 38-39 1.1849614328139353 1101 40-41 1.1527743066342637 1101 42-43 1.1832493280112146 1101 44-45 0.9229978222558373 1101 46-47 1.0492204080934755 1101 48-49 1.053099169154052 1101 50-51 0.9502001289322806 1101 52-53 0.7725532735686684 1101 54-55 0.848064029040188 1101 56-57 0.8379718330922685 1101 58-59 0.9183368006035124 1101 60-61 0.9046074483323281 1101 62-63 0.927366136426329 1101 64-65 0.811415972277004 1101 66-67 0.8875007932135759 1101 68-69 0.7866870069734517 1101 70-71 0.6985287776520153 1101 72-73 0.781283992593444 1101 74-75 0.7354950232245088 1101 76-77 0.8405075467029803 1101 78-79 0.664823426468061 1101 80-81 0.722396814413802 1101 82-83 0.7683349059215168 1101 84-85 0.9191367712108551 1101 86-87 0.8211032295266278 1101 88-89 0.7021521544855922 1101 90-91 0.761286143208391 1101 92-93 0.8758363274053309 1101 94-95 0.7925014134357227 1101 96-97 0.764577747896606 1101 98-99 0.7605135174850162 1101 100-101 0.7131804773586481 1101 102-103 0.6840558152751655 1101 104-105 0.639886001795265 1101 106-107 0.6717749401382278 1101 108-109 0.7498424246550108 1101 110-111 0.7721592100105426 1101 112-113 0.7421396104467561 1101 114-115 0.8273585038194966 1101 116-117 0.782618299254132 1101 118-119 0.7677676855914939 1101 120-121 0.6270036770550895 1101 122-123 0.6456243027419077 1101 124-125 0.3759090276294508 1102 1 0.24148964057316746 1102 2 0.16937626818491935 1102 3 0.32608655907499795 1102 4 0.3323735425743308 1102 5 0.2897834930281675 1102 6 0.2112593714205957 1102 7 0.3303476112428996 1102 8 0.3855483902480472 1102 9 0.7125585667306567 1102 10-11 0.39273728945929776 1102 12-13 0.43186797188357673 1102 14-15 0.552427639955674 1102 16-17 0.3944100062361997 1102 18-19 0.31658022478875125 1102 20-21 0.48445650247234084 1102 22-23 0.4853878050801015 1102 24-25 0.43095373747409127 1102 26-27 0.38041345729391907 1102 28-29 0.3167251208405162 1102 30-31 0.46421086087089947 1102 32-33 0.2900382694152981 1102 34-35 0.1863794167025219 1102 36-37 0.274189408523938 1102 38-39 0.34280020084798224 1102 40-41 0.349503780589977 1102 42-43 0.39297117722468045 1102 44-45 0.3676679185349343 1102 46-47 0.38956335160975186 1102 48-49 0.2959164238154841 1102 50-51 0.1214216388185605 1102 52-53 0.11374970544810736 1102 54-55 -0.049099449841904175 1102 56-57 0.1032128233426306 1102 58-59 0.2002090327677024 1102 60-61 0.19057044172689785 1102 62-63 0.22918045104008655 1102 64-65 0.2286736916379155 1102 66-67 0.18347667129097545 1102 68-69 0.3190395710135192 1102 70-71 0.26089894475107656 1102 72-73 0.21040080534659467 1102 74-75 0.247483894120343 1102 76-77 0.21001046858032169 1102 78-79 0.17467307365347295 1102 80-81 0.271610260810899 1102 82-83 0.2552213212822103 1102 84-85 0.26791842857520365 1102 86-87 0.2874692880634484 1102 88-89 0.2854197496412354 1102 90-91 0.15374579695580692 1102 92-93 0.26069524441243175 1102 94-95 0.33019051410495237 1102 96-97 0.29722236671710434 1102 98-99 0.20931270995428974 1102 100-101 0.11651366887479142 1102 102-103 0.11886119629216907 1102 104-105 0.16467867540092485 1102 106-107 0.16682596330495159 1102 108-109 0.1550973868533454 1102 110-111 0.16569364603163805 1102 112-113 0.07484303908419676 1102 114-115 0.031759696974809515 1102 116-117 0.1031455401208845 1102 118-119 0.12140126364812076 1102 120-121 0.0915905945963722 1102 122-123 0.03348464767086767 1102 124-125 -0.3095590533091688 1103 1 0.5310391486324981 1103 2 0.552936136673452 1103 3 0.7055981338316002 1103 4 0.5475184304912233 1103 5 0.5495249235528945 1103 6 0.4726220481015062 1103 7 0.5289298098549082 1103 8 0.6873018082925597 1103 9 1.146540564118773 1103 10-11 0.7944256443357034 1103 12-13 0.6562328206486114 1103 14-15 0.8408296663380455 1103 16-17 0.6840087536569825 1103 18-19 0.7798258906291196 1103 20-21 0.7174295458944471 1103 22-23 0.7181787943646256 1103 24-25 0.5941971239849551 1103 26-27 0.8012463535122478 1103 28-29 0.7597133919369483 1103 30-31 0.8980088316247645 1103 32-33 0.8083893557057422 1103 34-35 0.7162040047648546 1103 36-37 0.686870998759531 1103 38-39 0.7756978364527427 1103 40-41 0.8140700694821348 1103 42-43 0.803324563884587 1103 44-45 0.679678319290737 1103 46-47 0.638235496070152 1103 48-49 0.6053419042060852 1103 50-51 0.5517504993086959 1103 52-53 0.49414510482608875 1103 54-55 0.3647454750994896 1103 56-57 0.38176288633102473 1103 58-59 0.5350245634175224 1103 60-61 0.6085901924091601 1103 62-63 0.6027298258463105 1103 64-65 0.6148365251834562 1103 66-67 0.551793085473193 1103 68-69 0.632126370510548 1103 70-71 0.6996169066564661 1103 72-73 0.5491286735686174 1103 74-75 0.505571422710581 1103 76-77 0.5018137367297015 1103 78-79 0.4161336241582987 1103 80-81 0.4390105744817028 1103 82-83 0.45099059352444826 1103 84-85 0.4887536041230227 1103 86-87 0.5237912655629593 1103 88-89 0.5557816136535152 1103 90-91 0.5475023043684502 1103 92-93 0.41375075865108 1103 94-95 0.36944568182707727 1103 96-97 0.3845495431334065 1103 98-99 0.36733255999194014 1103 100-101 0.3509053757389893 1103 102-103 0.33385931584533424 1103 104-105 0.36093259738181516 1103 106-107 0.335986912896999 1103 108-109 0.27456632353736765 1103 110-111 0.4443497124524711 1103 112-113 0.3898429411899187 1103 114-115 0.404420644667411 1103 116-117 0.22949680992657306 1103 118-119 0.07551974903952186 1103 120-121 0.023199751539603852 1103 122-123 0.0758500043172603 1103 124-125 -0.15939331975518556 1104 1 0.515283832509148 1104 2 0.5489982899130865 1104 3 0.6963233849226 1104 4 0.5993718841995346 1104 5 0.5494396926996856 1104 6 0.47667837179257333 1104 7 0.6264573292955546 1104 8 0.7035645804532678 1104 9 1.119764475543974 1104 10-11 0.8673240917741083 1104 12-13 0.7835490521996746 1104 14-15 0.9053220697604516 1104 16-17 0.7635684305171964 1104 18-19 0.7102024840607157 1104 20-21 0.8151269225673659 1104 22-23 0.8961255528871774 1104 24-25 0.9667269026476859 1104 26-27 0.8568949225255125 1104 28-29 0.873445276224885 1104 30-31 0.9272175508014939 1104 32-33 0.895081525136213 1104 34-35 0.8078099444486142 1104 36-37 0.6869505626677039 1104 38-39 0.9254257777558976 1104 40-41 0.8380976897188432 1104 42-43 0.8468756408912625 1104 44-45 0.7296589610065212 1104 46-47 0.6633718729912061 1104 48-49 0.6845253714757789 1104 50-51 0.7311564583960717 1104 52-53 0.558899321108747 1104 54-55 0.5322818289296336 1104 56-57 0.6302603903061978 1104 58-59 0.623560681200523 1104 60-61 0.717211096756877 1104 62-63 0.6886983641764672 1104 64-65 0.5979002177164787 1104 66-67 0.6217462326828951 1104 68-69 0.6160126012244191 1104 70-71 0.6924480700787683 1104 72-73 0.7088682933120793 1104 74-75 0.7523551669503235 1104 76-77 0.7168204267472049 1104 78-79 0.6974381307245494 1104 80-81 0.7883172124237596 1104 82-83 0.708357017585417 1104 84-85 0.7017514754673506 1104 86-87 0.6614846557289482 1104 88-89 0.6999409880953209 1104 90-91 0.6396719917434908 1104 92-93 0.6529231156861464 1104 94-95 0.5612963277530234 1104 96-97 0.5440139004670854 1104 98-99 0.6418015219073467 1104 100-101 0.6315055188180168 1104 102-103 0.5063333166571446 1104 104-105 0.4972657696227891 1104 106-107 0.44416299983971896 1104 108-109 0.27734380663400415 1104 110-111 0.386587070707062 1104 112-113 0.4788726121051283 1104 114-115 0.47909979735183583 1104 116-117 0.5395281942237276 1104 118-119 0.46718725441404985 1104 120-121 0.3928964354851665 1104 122-123 0.3826337001990687 1104 124-125 0.09992782254376564 1105 1 0.28077906319288815 1105 2 0.4079845810881437 1105 3 0.45598104338105117 1105 4 0.3552321868202597 1105 5 0.3088367867529769 1105 6 0.21074596225736997 1105 7 0.3483565329909766 1105 8 0.4132862471242902 1105 9 0.8001508603130674 1105 10-11 0.45558897684254873 1105 12-13 0.4010879269855536 1105 14-15 0.1627597746789604 1105 16-17 0.31749967056266826 1105 18-19 0.28819969366865905 1105 20-21 0.6407517470059219 1105 22-23 0.6848624313488401 1105 24-25 0.6501463790962418 1105 26-27 0.6490931391262862 1105 28-29 0.5612548416981511 1105 30-31 0.7540373549744146 1105 32-33 0.605154096825558 1105 34-35 0.5383290514882972 1105 36-37 0.5178969866173233 1105 38-39 0.6428948782161044 1105 40-41 0.6295986714104842 1105 42-43 0.6565692177317644 1105 44-45 0.5897753086778721 1105 46-47 0.6054344915269496 1105 48-49 0.4941365960210007 1105 50-51 0.5700017310693113 1105 52-53 0.4343740087437311 1105 54-55 0.3737727598649627 1105 56-57 0.3823838304418885 1105 58-59 0.5194970819898259 1105 60-61 0.4842563515943823 1105 62-63 0.5507391702471267 1105 64-65 0.5405642113467337 1105 66-67 0.44021011110709196 1105 68-69 0.4490492774799719 1105 70-71 0.48611620979125547 1105 72-73 0.42056314712149856 1105 74-75 0.4457066642661047 1105 76-77 0.5421031333171129 1105 78-79 0.4409731124400196 1105 80-81 0.379583816868692 1105 82-83 0.4530107269099979 1105 84-85 0.5930186318324324 1105 86-87 0.5453897057111305 1105 88-89 0.534209390347506 1105 90-91 0.44795192680282625 1105 92-93 0.5272413853758238 1105 94-95 0.45839001794049494 1105 96-97 0.4645323161541661 1105 98-99 0.4841596210595114 1105 100-101 0.2927519665582956 1105 102-103 0.30179403490278034 1105 104-105 0.30946448295169304 1105 106-107 0.22634161456082325 1105 108-109 0.3021987315191268 1105 110-111 0.3522702011516472 1105 112-113 0.353478098133273 1105 114-115 0.28463610832339725 1105 116-117 0.2149103335649727 1105 118-119 0.1611486961108035 1105 120-121 0.05016201393959463 1105 122-123 0.016511953005799995 1105 124-125 -0.17370714095833506 1106 1 0.3669634418851402 1106 2 0.413394125626656 1106 3 0.49549082191993676 1106 4 0.2872274047872736 1106 5 0.26214010850514313 1106 6 0.17785700657043435 1106 7 0.08548843218980551 1106 8 0.37348437950947755 1106 9 0.7826340178810014 1106 10-11 0.233772555786679 1106 12-13 0.46211328854520417 1106 14-15 0.5981493968226559 1106 16-17 0.3900802405230763 1106 18-19 0.42561896463627136 1106 20-21 0.5281444145553493 1106 22-23 0.50042580123948 1106 24-25 0.3602304184714882 1106 26-27 0.4528280670557052 1106 28-29 0.47138575245656966 1106 30-31 0.4975206831333452 1106 32-33 0.5298435910534423 1106 34-35 0.4341523971122214 1106 36-37 0.3539437543294355 1106 38-39 0.5620331029946968 1106 40-41 0.524957622829902 1106 42-43 0.5243071016574845 1106 44-45 0.22249921346725898 1106 46-47 0.332447698341376 1106 48-49 0.41566597442253794 1106 50-51 0.3611203307531383 1106 52-53 0.3893183088653913 1106 54-55 0.32274719809402086 1106 56-57 0.2911500818392554 1106 58-59 0.4329608936617575 1106 60-61 0.43307260919007007 1106 62-63 0.5141928616496614 1106 64-65 0.5590167673044206 1106 66-67 0.4629181854913469 1106 68-69 0.5045437335470311 1106 70-71 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-1.5209721830182552 2216 84-85 -1.6725432499099142 2216 86-87 -1.6973095961691058 2216 88-89 -2.0923778501636576 2216 90-91 -1.7810662950350427 2216 92-93 -1.6765844043462153 2216 94-95 -1.6101938185554054 2216 96-97 -1.5308498074574892 2216 98-99 -1.5937248570914413 2216 100-101 -1.7134175509039338 2216 102-103 -1.956956271001932 2216 104-105 -1.6524683183210307 2216 106-107 -1.4291294058384167 2216 108-109 -1.5196472429049628 2216 110-111 -1.5964563310292696 2216 112-113 -1.7219524420141141 2216 114-115 -1.6799323848638217 2216 116-117 -1.6058444994042205 2216 118-119 -1.4893923958203743 2216 120-121 -1.4229091340646818 2216 122-123 -1.3917158526884705 2216 124-125 -1.337096978898117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38447.0 3 7571.0 4 5163.0 5 4510.0 6 4941.0 7 5772.0 8 6365.0 9 6907.0 10 7283.0 11 8353.0 12 9118.0 13 9805.0 14 9515.0 15 9244.0 16 8499.0 17 8013.0 18 7520.0 19 7559.0 20 7542.0 21 7897.0 22 9652.0 23 12916.0 24 18638.0 25 26587.0 26 31154.0 27 34047.0 28 40681.0 29 48628.0 30 58116.0 31 70282.0 32 88603.0 33 115374.0 34 157507.0 35 229035.0 36 405962.0 37 655098.0 38 236967.0 39 430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.47256487234829 18.098200111350376 12.023069216750207 24.406165799551125 2 15.98591886901308 20.170087763235983 38.82523064919006 25.01876271856088 3 18.72608174567095 25.068272926395835 29.113774434847294 27.09187089308592 4 12.56825361663578 16.02375052280245 36.84810873629762 34.55988712426415 5 13.700361950781637 38.56891818953525 33.968779162082704 13.761940697600414 6 32.61371244369137 35.97946026366905 17.720419886762823 13.68640740587676 7 29.50554634642875 32.324200386742 20.708591681368897 17.46166158546035 8 26.987332188276447 35.080399193941666 19.842449980906533 18.08981863687535 9 27.268083544724398 15.75824301169656 19.39991387312238 37.57375957045666 10-11 25.155783116918812 26.68012553991691 27.569928035877282 20.59416330728699 12-13 25.375100195131246 24.07871622941053 27.699379270626974 22.84680430483125 14-15 22.721016994964867 25.388132496335135 24.86099969457749 27.029850814122508 16-17 23.14651376256745 28.78941232239632 25.394799976690773 22.669273938345448 18-19 23.150421277287855 27.649157207758968 26.7097385123432 22.49068300260998 20-21 23.95794883707665 26.92621515973068 26.436174895868252 22.67966110732442 22-23 23.770544145384836 26.80283923504245 26.308409602247117 23.118207017325602 24-25 23.494453830137893 26.97499961251724 26.408551486125535 23.12199507121933 26-27 23.397522667276167 27.351674363297217 26.129189318099062 23.12161365132755 28-29 23.21064457526451 27.06993753108825 26.573228158354322 23.14618973529292 30-31 23.3163313917404 26.775050858050705 26.629508891969294 23.279108858239603 32-33 23.179132414222348 27.041146741046358 26.45736565141258 23.32235519331872 34-35 23.621828238774413 26.947897505158753 26.100138324476074 23.330135931590757 36-37 23.642205863360456 26.680139480821385 26.19780446855224 23.479850187265917 38-39 23.230596449755495 26.78776332116581 26.53545853450387 23.44618169457483 40-41 23.393457284222464 26.68161499842127 26.60834048307454 23.316587234281727 42-43 23.418765587310325 26.6147415117932 26.478440046676432 23.488052854220047 44-45 23.247905917982408 26.781002911133555 26.435419452852365 23.53567171803167 46-47 23.37229601110236 26.913135945208964 26.19016426234034 23.524403781348337 48-49 23.422583774970267 26.714975495995564 26.346201336845077 23.5162393921891 50-51 23.338640700861816 26.72770311529731 26.420523290595423 23.51313289324545 52-53 23.524518953377452 26.66880483534724 26.246592293665344 23.560083917609965 54-55 23.281258750478663 26.587402699843626 26.38112130532188 23.75021724435583 56-57 23.15239764244914 26.980338587949376 26.49828887437693 23.368974895224554 58-59 23.399446002613757 26.463046588849124 26.3845388273474 23.752968581189716 60-61 23.275665208689176 26.591422215195827 26.72490310287783 23.408009473237176 62-63 23.201304294632703 26.683129480464512 26.552890147607332 23.562676077295453 64-65 23.13991604053894 26.59982368265192 26.470375503047944 23.7898847737612 66-67 23.088113209613823 26.867712076343825 26.307215037961324 23.736959676081028 68-69 22.923622232266137 26.602898144583182 26.66382295761861 23.809656665532074 70-71 22.964671691043595 26.612356115371792 26.672365947561243 23.750606246023366 72-73 23.185064125853575 26.595189765324466 26.353082583452064 23.866663525369898 74-75 23.034634834885725 26.866104402565117 26.29368620537864 23.80557455717052 76-77 23.178503175763026 26.675706818054074 26.39082219364054 23.754967812542365 78-79 23.251491055115878 26.6531281211104 26.504234429139935 23.591146394633792 80-81 23.134441098539686 27.017385271529783 26.379782716655598 23.468390913274934 82-83 23.25177231530038 26.81684277183458 26.310062485197122 23.621322427667916 84-85 23.243488112465037 26.568633407773802 26.39954070934614 23.78833777041502 86-87 23.203484621993287 26.38250364617595 26.64838463606752 23.765627095763247 88-89 23.349638835436227 26.409829347134416 26.499751067084166 23.740780750345188 90-91 23.205584504314896 26.79315485723993 26.511639826089763 23.48962081235541 92-93 23.288665443817404 26.348509559468415 26.52664656873402 23.836178427980155 94-95 23.219246107600547 26.388176512477873 26.540396598833738 23.852180781087842 96-97 23.341226914169244 26.46601444568691 26.490647873620592 23.702110766523255 98-99 23.382463498513573 26.444137134081046 26.260673949261005 23.912725418144383 100-101 23.034125824874828 26.446646315001125 26.588744255944924 23.930483604179116 102-103 23.219504251308624 26.168978125646458 26.598376519442684 24.01314110360223 104-105 23.124669088426188 26.348703611932116 26.63405736751241 23.892569932129287 106-107 23.166186728495518 26.41139535436382 26.528316352097935 23.89410156504273 108-109 23.325961676753618 26.46286949894777 26.19210473676119 24.019064087537423 110-111 23.15242178047387 26.663484260002623 26.444063520010413 23.74003043951309 112-113 23.14697307955158 26.551278259940513 26.551629575026542 23.750119085481366 114-115 23.010927175174146 26.740331514548554 26.331611267510162 23.917130042767138 116-117 22.919476811410604 26.705879702831265 26.50893277289911 23.865710712859023 118-119 23.010362959331996 26.815609485770164 26.174709293114923 23.99931826178292 120-121 23.056568581167948 26.934729641354483 26.280174085155885 23.728527692321684 122-123 23.104065004017073 26.977175548836207 26.2362856831637 23.682473763983015 124-125 23.23286739473715 27.06919734951857 26.03334559487534 23.664589660868945 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 704.0 1 569.0 2 384.5 3 292.5 4 236.5 5 205.5 6 173.0 7 162.0 8 157.0 9 166.0 10 173.5 11 161.5 12 157.0 13 163.0 14 193.0 15 219.0 16 275.0 17 340.5 18 432.5 19 586.5 20 786.0 21 1068.0 22 1500.5 23 2075.0 24 2885.5 25 4225.0 26 6036.5 27 8579.5 28 11512.5 29 14973.0 30 19603.0 31 24257.5 32 29050.5 33 34722.0 34 40507.5 35 45166.0 36 51670.5 37 58912.5 38 63590.5 39 67030.0 40 69675.0 41 74865.5 42 80397.0 43 85551.0 44 91731.5 45 96372.5 46 97408.5 47 97299.5 48 98237.0 49 96869.0 50 94832.0 51 92850.5 52 92025.0 53 91337.0 54 90027.5 55 85631.0 56 76816.0 57 69009.5 58 63152.5 59 56557.0 60 47891.5 61 39173.0 62 31528.0 63 24746.5 64 19661.5 65 15818.5 66 11916.5 67 9682.0 68 7185.5 69 4365.5 70 3286.5 71 2542.0 72 2050.0 73 1631.0 74 1260.0 75 1032.0 76 708.5 77 374.5 78 202.5 79 149.5 80 96.0 81 58.5 82 38.0 83 23.0 84 15.5 85 13.0 86 11.0 87 7.5 88 7.0 89 7.0 90 4.0 91 3.5 92 4.0 93 3.5 94 3.5 95 2.5 96 1.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08707687437414788 2 3.719467818544523E-4 3 4.132742020605025E-5 4 0.0020250435900964624 5 0.0014464597072117588 6 4.132742020605025E-5 7 0.0 8 0.0010331855051512564 9 6.199113030907538E-4 10-11 0.009567297777700634 12-13 0.001963052459787387 14-15 0.004215396861017126 16-17 0.0023969903719509144 18-19 0.013741367218511708 20-21 0.016944242284480604 22-23 0.010187209080791387 24-25 0.009856589719142985 26-27 0.010083890530276262 28-29 0.007335617086573919 30-31 0.01932056894632849 32-33 0.001818406489066211 34-35 0.001157167765769407 36-37 5.579201727816783E-4 38-39 3.5128307175142715E-4 40-41 0.0013638048667996582 42-43 0.002665618603290241 44-45 0.0010331855051512564 46-47 0.0014464597072117588 48-49 0.008203492910900974 50-51 0.004484025092356452 52-53 0.007645572738119297 54-55 0.007872873549252573 56-57 0.00995990826965811 58-59 0.008058846940179798 60-61 0.010476501022233738 62-63 0.012770172843669526 64-65 0.008265484041210051 66-67 0.013658712378099609 68-69 0.013452075277069358 70-71 0.004029423470089899 72-73 0.013142119625523979 74-75 0.01305946478511188 76-77 0.005165927525756282 78-79 0.007831546129046523 80-81 0.013452075277069358 82-83 0.016861587444068505 84-85 0.013142119625523979 86-87 0.015931720489432373 88-89 0.015477118867165818 90-91 0.012067606700166673 92-93 0.008885395344300804 94-95 0.007356280796676945 96-97 0.00927800583625828 98-99 0.005868493669259136 100-101 0.01004256311007021 102-103 0.011137739745530542 104-105 0.010249200211100463 106-107 0.004897299294416954 108-109 0.003016901675041668 110-111 0.006137121900598462 112-113 0.00911269615543408 114-115 0.013224774465936081 116-117 0.011055084905118442 118-119 0.21839475207887254 120-121 0.027069460234962914 122-123 0.026883486844035687 124-125 0.23345859674397787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2419701.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.70576783871608 #Duplication Level Percentage of deduplicated Percentage of total 1 85.34064669805488 60.340759526385604 2 8.7099602264986 12.31688851318522 3 2.694623304443077 5.715762293302385 4 1.1337840426172094 3.206602851861335 5 0.6121140027678704 2.163999528526613 6 0.3724100739409273 1.5798924137319776 7 0.22780594447645808 1.1275035955702326 8 0.1545455824091707 0.874181125625759 9 0.1137586090684838 0.723905082220633 >10 0.5771047264632316 7.4555622986789345 >50 0.04507350391923832 2.207628422244478 >100 0.01790603171370461 2.1462529248690774 >500 2.6725362712122844E-4 0.1410614237976977 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 8.26548404121005E-5 9 0.0 0.0 0.0 0.0 8.26548404121005E-5 10-11 0.0 0.0 0.0 0.0 8.26548404121005E-5 12-13 0.0 0.0 0.0 0.0 8.26548404121005E-5 14-15 0.0 0.0 0.0 0.0 2.0663710103025124E-4 16-17 0.0 0.0 0.0 0.0 2.479645212363015E-4 18-19 0.0 0.0 0.0 0.0 2.479645212363015E-4 20-21 0.0 0.0 0.0 4.132742020605025E-5 2.479645212363015E-4 22-23 0.0 0.0 0.0 1.0331855051512562E-4 2.479645212363015E-4 24-25 0.0 0.0 0.0 4.752653323695779E-4 2.479645212363015E-4 26-27 0.0 0.0 0.0 0.0010538492152542813 2.8929194144235176E-4 28-29 0.0 0.0 0.0 0.0035128307175142717 2.8929194144235176E-4 30-31 0.0 0.0 0.0 0.010331855051512562 2.8929194144235176E-4 32-33 0.0 0.0 0.0 0.028040654609805094 3.30619361648402E-4 34-35 0.0 0.0 0.0 0.05686653020352515 4.1327420206050254E-4 36-37 0.0 0.0 0.0 0.10478567393244041 4.1327420206050254E-4 38-39 0.0 0.0 0.0 0.17948498595487625 4.1327420206050254E-4 40-41 0.0 0.0 0.0 0.2585030133888443 4.1327420206050254E-4 42-43 0.0 0.0 0.0 0.3380582972854911 4.1327420206050254E-4 44-45 0.0 0.0 0.0 0.43189220486332813 4.3393791216352767E-4 46-47 0.0 0.0 0.0 0.54686508787656 4.5460162226655275E-4 48-49 0.0 0.0 0.0 0.6714672597978015 4.5460162226655275E-4 50-51 0.0 0.0 0.0 0.8045208891511803 4.5460162226655275E-4 52-53 0.0 0.0 0.0 0.9380497838369286 4.5460162226655275E-4 54-55 0.0 0.0 0.0 1.0802161093457414 4.5460162226655275E-4 56-57 0.0 0.0 0.0 1.2536053008202255 4.5460162226655275E-4 58-59 0.0 0.0 0.0 1.4438147523185716 4.5460162226655275E-4 60-61 0.0 0.0 0.0 1.6504311896387198 4.5460162226655275E-4 62-63 0.0 0.0 0.0 1.8615109883411214 4.5460162226655275E-4 64-65 0.0 0.0 0.0 2.069367248267451 4.5460162226655275E-4 66-67 0.0 0.0 0.0 2.3042103135883316 4.5460162226655275E-4 68-69 0.0 0.0 0.0 2.5745123054460035 4.5460162226655275E-4 70-71 0.0 0.0 0.0 2.870354642991014 4.5460162226655275E-4 72-73 0.0 0.0 0.0 3.166403617637055 4.95929042472603E-4 74-75 0.0 0.0 0.0 3.4666059980138044 4.95929042472603E-4 76-77 0.0 0.0 0.0 3.7965434572288066 4.95929042472603E-4 78-79 0.0 0.0 0.0 4.160865330055242 4.95929042472603E-4 80-81 0.0 0.0 0.0 4.561348695561972 5.165927525756281E-4 82-83 0.0 0.0 0.0 4.977763781558135 5.372564626786533E-4 84-85 0.0 0.0 0.0 5.393765593352237 5.372564626786533E-4 86-87 0.0 0.0 0.0 5.81518129719333 5.372564626786533E-4 88-89 0.0 0.0 0.0 6.269948229140708 5.372564626786533E-4 90-91 0.0 0.0 0.0 6.7804038598157375 5.372564626786533E-4 92-93 0.0 0.0 0.0 7.31991266689562 5.372564626786533E-4 94-95 0.0 0.0 0.0 7.856569881981287 5.785838828847035E-4 96-97 0.0 0.0 0.0 8.397235856826939 5.785838828847035E-4 98-99 4.132742020605025E-5 0.0 0.0 8.950919969037496 5.785838828847035E-4 100-101 4.132742020605025E-5 0.0 0.0 9.568558264016918 5.785838828847035E-4 102-103 4.132742020605025E-5 0.0 0.0 10.227069377580122 5.992475929877286E-4 104-105 4.132742020605025E-5 0.0 0.0 10.882894208829935 6.199113030907538E-4 106-107 4.132742020605025E-5 0.0 0.0 11.528862450360602 6.199113030907538E-4 108-109 4.132742020605025E-5 0.0 0.0 12.189625908325038 6.199113030907538E-4 110-111 4.132742020605025E-5 0.0 0.0 12.905561472264548 6.819024333998291E-4 112-113 4.132742020605025E-5 0.0 0.0 13.662721137859595 7.025661435028543E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2600 0.0 53.357346 1 GTATCAA 3695 0.0 48.66359 1 GTACATG 12205 0.0 41.027008 1 TCAACGC 4355 0.0 39.739517 4 ATCAACG 4385 0.0 39.332012 3 CAACGCA 4410 0.0 39.113083 5 TACATGG 12660 0.0 39.084755 2 AACGCAG 4575 0.0 37.702446 6 ACATGGG 12880 0.0 36.985744 3 TATCAAC 4850 0.0 36.664623 2 CATGGGA 8405 0.0 33.46888 4 ACGCAGA 5620 0.0 30.48027 7 CGCAGAG 5735 0.0 29.972782 8 CATGGGG 5560 0.0 29.736357 4 ATGGGAG 3890 0.0 27.828156 5 AGTACAT 8810 0.0 25.382225 2 GAGTACA 9445 0.0 25.0265 1 GCAGAGT 6915 0.0 23.826443 9 ATGGGGA 3295 0.0 22.925083 5 GTATAGG 765 0.0 22.570864 1 >>END_MODULE