FastQCFastQC Report
Thu 26 May 2016
SRR1364415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419701
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.12332102189485394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27520.11373306040705031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35950.062.3245741
GTATCAA59150.055.408741
TCAACGC77900.041.5883034
ATCAACG77900.041.5118563
CAACGCA79600.040.6639445
TATCAAC82000.039.3685232
AACGCAG82550.039.2099726
GTACATG122550.036.2740361
TACATGG125800.035.036022
ACATGGG127000.033.9034843
ACGCAGA101900.031.6475287
CGCAGAG102900.031.3373838
CATGGGA83200.029.9916574
GCAGAGT118700.026.8653729
CATGGGG57750.026.0902544
ATGGGAG36750.025.905165
GTGGTAT19150.025.827341
AGTACAT91900.024.3689042
GAGTACA96450.024.2806721
GAGTACT58550.023.47274612-13
Produced by FastQC (version 0.11.5)