##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364415_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2419701 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95715421037558 33.0 31.0 34.0 30.0 34.0 2 31.89251853844752 33.0 31.0 34.0 30.0 34.0 3 32.10139889184656 33.0 31.0 34.0 30.0 34.0 4 35.78946324359911 37.0 35.0 37.0 35.0 37.0 5 35.73027659202521 37.0 35.0 37.0 33.0 37.0 6 35.79135562617034 37.0 35.0 37.0 33.0 37.0 7 35.70189209327929 37.0 35.0 37.0 33.0 37.0 8 35.72225700613423 37.0 35.0 37.0 33.0 37.0 9 37.37758632161577 39.0 37.0 39.0 34.0 39.0 10-11 37.320653254265714 39.0 37.0 39.0 34.0 39.0 12-13 37.29145067923682 39.0 37.0 39.0 33.0 39.0 14-15 38.6073853753005 40.0 38.0 41.0 34.0 41.0 16-17 38.61765751222981 40.0 38.0 41.0 34.0 41.0 18-19 38.6654706511259 40.0 38.0 41.0 34.0 41.0 20-21 38.66741386642399 40.0 38.0 41.0 34.0 41.0 22-23 38.63051509256722 40.0 38.0 41.0 34.0 41.0 24-25 38.573315050082634 40.0 38.0 41.0 34.0 41.0 26-27 38.46197009465219 40.0 38.0 41.0 34.0 41.0 28-29 38.38669715803729 40.0 38.0 41.0 34.0 41.0 30-31 38.281933800911766 40.0 38.0 41.0 33.5 41.0 32-33 38.164876569460446 40.0 38.0 41.0 33.0 41.0 34-35 38.06710188572886 40.0 37.0 41.0 33.0 41.0 36-37 37.978010919530966 40.0 37.0 41.0 33.0 41.0 38-39 37.88737038171245 40.0 37.0 41.0 33.0 41.0 40-41 37.77011891138616 40.0 37.0 41.0 33.0 41.0 42-43 37.63925501539239 40.0 37.0 41.0 32.5 41.0 44-45 37.463752133011475 40.0 37.0 41.0 32.0 41.0 46-47 37.251448629396776 40.0 36.5 41.0 31.0 41.0 48-49 37.037084333973496 40.0 36.0 41.0 31.0 41.0 50-51 36.82099771831313 39.5 36.0 41.0 30.5 41.0 52-53 36.690299751911496 39.0 35.5 41.0 30.0 41.0 54-55 36.53625861211778 39.0 35.0 41.0 30.0 41.0 56-57 36.32220261924924 39.0 35.0 41.0 30.0 41.0 58-59 36.107085544866905 38.5 35.0 40.5 29.5 41.0 60-61 35.86754747797352 38.0 35.0 40.0 29.0 41.0 62-63 35.904475387661535 38.0 35.0 40.0 30.0 41.0 64-65 35.695643387344134 37.5 35.0 40.0 29.5 41.0 66-67 35.419026565678976 37.0 34.5 40.0 29.0 41.0 68-69 35.102578583056335 36.5 34.0 39.0 29.0 41.0 70-71 34.77333232494428 36.0 34.0 39.0 29.0 41.0 72-73 34.42558378080598 35.5 34.0 39.0 29.0 40.5 74-75 34.08489396003887 35.0 34.0 37.5 29.0 39.5 76-77 33.77124591013518 35.0 34.0 37.0 29.0 39.0 78-79 33.45544449500166 35.0 33.5 36.5 28.0 39.0 80-81 33.17140072265127 35.0 33.0 36.0 28.0 38.0 82-83 32.92009177993479 35.0 33.0 36.0 27.5 37.0 84-85 32.702770300958676 35.0 33.0 35.0 27.0 37.0 86-87 32.52319005530022 35.0 33.0 35.0 27.0 36.0 88-89 32.35942519344332 35.0 33.0 35.0 27.0 36.0 90-91 32.241762928560185 35.0 33.0 35.0 27.0 36.0 92-93 32.128818601967765 35.0 33.0 35.0 26.5 36.0 94-95 32.027176704890394 35.0 33.0 35.0 26.0 35.0 96-97 31.98037154177314 35.0 33.0 35.0 26.0 35.0 98-99 31.91061230292503 35.0 33.0 35.0 26.0 35.0 100-101 31.908910439760945 35.0 33.0 35.0 26.0 35.0 102-103 30.779876315296807 33.5 30.5 34.5 24.5 35.0 104-105 31.755141234392184 35.0 32.5 35.0 25.5 35.0 106-107 31.946899637599852 35.0 33.0 35.0 27.0 35.0 108-109 31.949646051309646 35.0 33.0 35.0 27.0 35.0 110-111 31.901643012917713 35.0 33.0 35.0 27.0 35.0 112-113 31.850053581000296 35.0 33.0 35.0 25.5 35.0 114-115 31.747885172589505 35.0 33.0 35.0 25.0 35.0 116-117 31.662991625824844 35.0 33.0 35.0 25.0 35.0 118-119 31.56406349379531 35.0 33.0 35.0 25.0 35.0 120-121 31.442826613701442 35.0 33.0 35.0 25.0 35.0 122-123 31.26375304221472 35.0 33.0 35.0 24.5 35.0 124-125 30.246539138513395 34.5 31.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3427735139016299 1101 2 0.5610399214774304 1101 3 0.40864046948932753 1101 4 0.2739577336652417 1101 5 0.2646649044342908 1101 6 0.2813538041873258 1101 7 0.3598613049518846 1101 8 0.37144937378574383 1101 9 0.4724339250773042 1101 10-11 0.40134351175633753 1101 12-13 0.4340057590042221 1101 14-15 0.4943459449678187 1101 16-17 0.5074834441639169 1101 18-19 0.36375909205359136 1101 20-21 0.3262399385463084 1101 22-23 0.3786494327563119 1101 24-25 0.4411375813930434 1101 26-27 0.35970888805173473 1101 28-29 0.42019188699073595 1101 30-31 0.38152734854065073 1101 32-33 0.3622356515018481 1101 34-35 0.3914941703195325 1101 36-37 0.39271570714655724 1101 38-39 0.42083715128095633 1101 40-41 0.40520360246094356 1101 42-43 0.4580352043872722 1101 44-45 0.47857516184898685 1101 46-47 0.34554263826733234 1101 48-49 0.21328520104515292 1101 50-51 0.2848667397636646 1101 52-53 0.3978612465958662 1101 54-55 0.2853478481971834 1101 56-57 0.32024945509740377 1101 58-59 0.20242273464318572 1101 60-61 0.15879731053736634 1101 62-63 0.2878063831301816 1101 64-65 0.22138473798541014 1101 66-67 0.2224465923436867 1101 68-69 0.3610056583275423 1101 70-71 0.35394711866529605 1101 72-73 0.4178276013598534 1101 74-75 0.2909080404097537 1101 76-77 0.24459964698981906 1101 78-79 0.3217090281815729 1101 80-81 0.24262692731699786 1101 82-83 0.266986536966634 1101 84-85 0.16013497595344006 1101 86-87 0.3068525340593098 1101 88-89 0.2744196285462266 1101 90-91 0.2589314827791611 1101 92-93 0.31640884665686997 1101 94-95 0.34943520850379883 1101 96-97 0.22038372486806068 1101 98-99 0.36288935462403415 1101 100-101 0.3042404641675027 1101 102-103 0.44780673728514486 1101 104-105 0.36320034851170746 1101 106-107 0.34824709431626033 1101 108-109 0.4378328417653137 1101 110-111 0.47084272761675194 1101 112-113 0.39784521762284797 1101 114-115 0.3841375505024196 1101 116-117 0.3666845271671235 1101 118-119 0.40126240639104793 1101 120-121 0.37009686171049694 1101 122-123 0.3853596712938483 1101 124-125 0.361965622701657 1102 1 0.29627948603041787 1102 2 0.4460634055181174 1102 3 0.24417994652070263 1102 4 0.21715758452082667 1102 5 0.18172666493519074 1102 6 0.2369413867921466 1102 7 0.25727045257442427 1102 8 0.2492193516179455 1102 9 0.21519895851218962 1102 10-11 0.26742767907817466 1102 12-13 0.3004595373835386 1102 14-15 0.32721342359700145 1102 16-17 0.29180652854603295 1102 18-19 0.285119316881115 1102 20-21 0.31407364843384045 1102 22-23 0.2485196759343964 1102 24-25 0.3364901581375648 1102 26-27 0.23829941129512378 1102 28-29 0.22150112558309587 1102 30-31 0.25457577408912613 1102 32-33 0.2689549500740185 1102 34-35 0.1993236759182082 1102 36-37 0.20153593878720955 1102 38-39 0.12185016921285552 1102 40-41 0.1403365811922086 1102 42-43 0.13440268405053502 1102 44-45 0.09201118855670387 1102 46-47 0.0356361755692447 1102 48-49 0.02906503753583678 1102 50-51 0.05080056608900918 1102 52-53 0.0872979930258424 1102 54-55 -6.30730315911876E-4 1102 56-57 -0.01483945689587074 1102 58-59 -0.10291300426427341 1102 60-61 -0.04672202254364777 1102 62-63 0.03523011133280107 1102 64-65 0.07519965000409456 1102 66-67 0.04300396936380224 1102 68-69 -0.06608050950305966 1102 70-71 -0.04630502048455298 1102 72-73 -0.029480073107052363 1102 74-75 -0.0277781621660651 1102 76-77 0.04756910695291339 1102 78-79 -0.03671715848116719 1102 80-81 -0.053703198288715726 1102 82-83 -0.03651828289746106 1102 84-85 -0.13028986699140432 1102 86-87 -0.018871568274796857 1102 88-89 -0.01543757631372955 1102 90-91 -0.0783695558249562 1102 92-93 -0.13023627561466355 1102 94-95 -0.08036247449872747 1102 96-97 -0.15456623286746662 1102 98-99 -0.1629032779137347 1102 100-101 -0.1586446470659304 1102 102-103 -0.16632360275998437 1102 104-105 -0.2696843549972918 1102 106-107 -0.3288715732875822 1102 108-109 -0.22825924368309103 1102 110-111 -0.236281798809852 1102 112-113 -0.26501727672292574 1102 114-115 -0.38250964584554836 1102 116-117 -0.2315505431018181 1102 118-119 -0.3376836820987421 1102 120-121 -0.34071033220294566 1102 122-123 -0.3673758493924062 1102 124-125 -0.609014295688457 1103 1 0.25066601180335013 1103 2 0.3376548421356631 1103 3 0.18963208513081753 1103 4 0.12985941111119104 1103 5 0.1150013396266587 1103 6 0.16882172550051422 1103 7 0.17153391208812963 1103 8 0.25325639481328466 1103 9 0.2768596429130099 1103 10-11 0.21009560210345768 1103 12-13 0.21249568287372256 1103 14-15 0.3272213135836992 1103 16-17 0.3413109351035999 1103 18-19 0.2743068937171742 1103 20-21 0.24722829304983662 1103 22-23 0.18588187512499843 1103 24-25 0.23742669955896645 1103 26-27 0.19800283838390698 1103 28-29 0.2856171300120067 1103 30-31 0.3454947208401933 1103 32-33 0.3377240741598371 1103 34-35 0.2798870881796489 1103 36-37 0.24136429943207816 1103 38-39 0.1450378173510103 1103 40-41 0.23211568260454385 1103 42-43 0.2246507627657266 1103 44-45 0.21971500663816101 1103 46-47 0.2106972279941104 1103 48-49 0.18112838594684177 1103 50-51 0.25265491751895297 1103 52-53 0.1946717992635456 1103 54-55 0.075769033685809 1103 56-57 -0.05417096754487005 1103 58-59 -0.14010238352394566 1103 60-61 0.012864106742838999 1103 62-63 0.12251330837879237 1103 64-65 0.21168663050325875 1103 66-67 0.08439527792978652 1103 68-69 0.15430391402674815 1103 70-71 0.12938905726220185 1103 72-73 0.05811002981052127 1103 74-75 0.12559879597522183 1103 76-77 0.10305938342096255 1103 78-79 0.09219609228394177 1103 80-81 0.03921352231721187 1103 82-83 0.06309272614709016 1103 84-85 0.19691518704185285 1103 86-87 0.18674374987138975 1103 88-89 0.18214325148613852 1103 90-91 0.19179798073709975 1103 92-93 0.14467336212097592 1103 94-95 0.20128418910350376 1103 96-97 0.171832362247347 1103 98-99 0.03266165828403089 1103 100-101 0.12356125587145428 1103 102-103 0.13596034383474276 1103 104-105 0.17914908124679485 1103 106-107 0.11486748848779982 1103 108-109 0.1540741702060302 1103 110-111 0.11299162108150185 1103 112-113 0.014171656021947143 1103 114-115 0.08727659980363356 1103 116-117 0.14858141838686834 1103 118-119 0.020379475199245434 1103 120-121 0.011418532319847685 1103 122-123 0.043172303050322824 1103 124-125 -0.12805685164672553 1104 1 0.2653826902421521 1104 2 0.43279227084171623 1104 3 0.27099536169496474 1104 4 0.17749559988967434 1104 5 0.18146976900035128 1104 6 0.24873909993083032 1104 7 0.2603588173897009 1104 8 0.24513649374151925 1104 9 0.2587799726173827 1104 10-11 0.33169177046280396 1104 12-13 0.313220794935674 1104 14-15 0.3211839770297331 1104 16-17 0.342613350189346 1104 18-19 0.37909905888609785 1104 20-21 0.3386777494915876 1104 22-23 0.31535479483481055 1104 24-25 0.2974117660254407 1104 26-27 0.302218561904688 1104 28-29 0.33381819987078387 1104 30-31 0.19205802847431386 1104 32-33 0.24614941601262785 1104 34-35 0.2450043969640774 1104 36-37 0.19011758663798872 1104 38-39 0.08211409987134033 1104 40-41 0.1944518058882494 1104 42-43 0.12801862063934522 1104 44-45 0.0927266267467175 1104 46-47 0.24838951499480544 1104 48-49 0.11806684836411563 1104 50-51 0.22557984092452443 1104 52-53 0.1868330542243939 1104 54-55 0.2483108111601453 1104 56-57 0.21190230197412774 1104 58-59 0.1286250563679019 1104 60-61 0.12024009660702006 1104 62-63 0.36644098788418944 1104 64-65 0.43835544003074034 1104 66-67 0.39215361279697447 1104 68-69 0.39970107015728473 1104 70-71 0.4252572347515269 1104 72-73 0.3503870264565947 1104 74-75 0.3421794610841644 1104 76-77 0.40200705439722384 1104 78-79 0.41250504808207467 1104 80-81 0.3705981821539268 1104 82-83 0.3092750941805704 1104 84-85 0.2447120903812987 1104 86-87 0.34540974798965607 1104 88-89 0.444955836395863 1104 90-91 0.4063886414303468 1104 92-93 0.42295942708804546 1104 94-95 0.33464803326886283 1104 96-97 0.3899525474219523 1104 98-99 0.31023595495571143 1104 100-101 0.25573300148093026 1104 102-103 0.30034957863395206 1104 104-105 0.3290654673619038 1104 106-107 0.3852841313532984 1104 108-109 0.3159422945016317 1104 110-111 0.38115229091138403 1104 112-113 0.26185848462118955 1104 114-115 0.34336917018180557 1104 116-117 0.22240592020195038 1104 118-119 0.12611038870171853 1104 120-121 0.34135169863697357 1104 122-123 0.1942319764348106 1104 124-125 0.0547516923534026 1105 1 0.2536859047122242 1105 2 0.3941270693777952 1105 3 0.2660699101298789 1105 4 0.12710412874071864 1105 5 0.15707010561354195 1105 6 0.13121743633894312 1105 7 0.12183252551167101 1105 8 0.07619330172007466 1105 9 0.1789419640847001 1105 10-11 0.17831439680676198 1105 12-13 0.23358953859403186 1105 14-15 0.14186120047634176 1105 16-17 0.1793600917544822 1105 18-19 0.21262512854364957 1105 20-21 0.22238664995917645 1105 22-23 0.27652764838105526 1105 24-25 0.3053862132846774 1105 26-27 0.16072112412133777 1105 28-29 0.19979252628855448 1105 30-31 0.18732226314843103 1105 32-33 0.18984528245027832 1105 34-35 0.2026302921277221 1105 36-37 0.06577818421645532 1105 38-39 0.13055663931983474 1105 40-41 0.10271243826673526 1105 42-43 0.039620766388672735 1105 44-45 0.03554334249070479 1105 46-47 -0.003036762852829611 1105 48-49 -0.07382968578071569 1105 50-51 -0.043826107089827815 1105 52-53 -0.03457566675295709 1105 54-55 -0.0777774083367504 1105 56-57 -0.18188153762974224 1105 58-59 -0.14458129756919647 1105 60-61 -0.10062759738637794 1105 62-63 0.058151176359110934 1105 64-65 0.171966534439008 1105 66-67 0.10080680564048805 1105 68-69 0.10899819404330913 1105 70-71 0.07351177276967746 1105 72-73 0.0033819049281333946 1105 74-75 0.10442729530593908 1105 76-77 0.14956676852583684 1105 78-79 0.12672108763673862 1105 80-81 0.14617256399610312 1105 82-83 0.1087719463475949 1105 84-85 0.0213797098364239 1105 86-87 0.10403270141893017 1105 88-89 0.19330537729997133 1105 90-91 0.06095474940858736 1105 92-93 0.13186352573418247 1105 94-95 -0.09176321927904496 1105 96-97 0.06320449286784324 1105 98-99 0.2190184423552317 1105 100-101 0.14604410143744317 1105 102-103 0.11594906412818418 1105 104-105 0.15606166811791766 1105 106-107 0.1368697398321892 1105 108-109 0.10026076172061948 1105 110-111 0.2259423245117027 1105 112-113 0.26124614374485233 1105 114-115 0.22006461803159638 1105 116-117 0.17916232327446124 1105 118-119 0.05211162267896441 1105 120-121 0.07408712816301843 1105 122-123 0.07732221799282613 1105 124-125 -0.04891466959524848 1106 1 0.20294536976959066 1106 2 0.2971925785123659 1106 3 0.17098133994566922 1106 4 0.12852521751714363 1106 5 0.11326593200347901 1106 6 0.0036563773802811284 1106 7 0.09833375365709429 1106 8 0.12042587264149773 1106 9 0.16352281488943987 1106 10-11 0.22469190260192562 1106 12-13 0.19614207348839585 1106 14-15 0.22267210008875793 1106 16-17 0.24365362838546645 1106 18-19 0.21527100303914182 1106 20-21 0.2221280894180282 1106 22-23 0.29180994081679756 1106 24-25 0.26783171741671197 1106 26-27 0.17991089645092728 1106 28-29 0.2591810919748596 1106 30-31 0.16095210483340594 1106 32-33 0.18333457580864376 1106 34-35 0.20446805351316044 1106 36-37 0.21321611048823286 1106 38-39 0.167698919336992 1106 40-41 0.12451270528826797 1106 42-43 0.15678167154117517 1106 44-45 0.13767722460410425 1106 46-47 0.09889855354134625 1106 48-49 0.17596304068019464 1106 50-51 0.16313004224915062 1106 52-53 0.028607999955028163 1106 54-55 0.06576400852289765 1106 56-57 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118-119 -0.674199155432035 2216 120-121 -0.8173943704727868 2216 122-123 -0.7689403192215103 2216 124-125 -0.6036504388299697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86.0 3 10.0 4 50.0 5 131.0 6 398.0 7 867.0 8 1467.0 9 2586.0 10 4038.0 11 5545.0 12 6854.0 13 9734.0 14 13430.0 15 10402.0 16 5660.0 17 5299.0 18 5474.0 19 5366.0 20 5501.0 21 5779.0 22 6516.0 23 7812.0 24 10548.0 25 14506.0 26 19991.0 27 26279.0 28 33951.0 29 43608.0 30 55085.0 31 68806.0 32 85959.0 33 110696.0 34 147613.0 35 216114.0 36 405922.0 37 760292.0 38 316577.0 39 749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72797290346695 18.76919489360013 12.443169884214583 25.05966231871834 2 16.033474159116327 20.740653794236753 37.88442256331581 25.341449483331115 3 19.01750482295977 24.97607117647156 29.489898647587527 26.51652535298114 4 13.133926736087703 16.39742129954955 36.314437008412995 34.154214955949755 5 13.477168154320934 38.442441067275695 34.07040309871833 14.009987679685038 6 32.75371626494348 36.1917443518848 17.138398504608627 13.916140878563096 7 29.004940189118354 32.50341076806653 20.781486421196067 17.71016262161904 8 27.18625152446521 34.67684643681182 19.613828320110624 18.52307371861234 9 27.405534816078514 14.839767392748113 19.277299137372758 38.47739865380061 10-11 25.420744133262748 26.142217571509867 27.528752519422856 20.90828577580453 12-13 25.806390954915503 23.485876974055884 28.059644559389774 22.648087511638835 14-15 22.863878636244724 24.860406306399014 25.49961751472599 26.776097542630268 16-17 22.83201519526586 28.386234497568086 26.045242779996368 22.73650752716968 18-19 22.726447117275146 26.852725659829108 28.053040492441518 22.367786730454227 20-21 23.497159359772137 26.09208741079993 27.910638545836864 22.50011468359107 22-23 23.316041940719124 26.085041085654797 27.7790727036109 22.81984427001518 24-25 23.1246546577449 26.24747851077468 27.883941032383753 22.743925799096665 26-27 22.96289683868072 26.657177832165 27.685286437992367 22.69463889116191 28-29 22.75353837672125 26.36822674705736 28.083720057544724 22.79451481867666 30-31 22.909351649804933 26.09574406533095 28.049948753554187 22.944955531309922 32-33 22.803747239844924 26.289591151964643 27.934401812455338 22.97225979573509 34-35 23.20871463044401 26.270063945917283 27.577188255904346 22.94403316773436 36-37 23.31151658820656 26.018565930253367 27.544684239912286 23.12523324162779 38-39 22.975607316771782 26.14372602234739 27.82664056426806 23.054026096612766 40-41 23.00517156122794 26.013446844421907 27.89183921563036 23.08954237871979 42-43 23.165388330114588 25.98317712128169 27.651950815004128 23.199483733599592 44-45 22.995630451861615 26.217206175473745 27.52079699103319 23.26636638163145 46-47 23.24340899970699 26.37646138923776 27.03579905120509 23.344330559850164 48-49 23.34313206466419 26.251879881026625 26.996847957660886 23.408140096648307 50-51 23.35951838677589 26.326434547078335 26.97085301035128 23.3431940557945 52-53 23.466535741399454 26.34932993787249 26.77570493213831 23.408429388589745 54-55 23.147054119496584 26.27593243958654 26.92297519404257 23.654038246874304 56-57 23.077903813258647 26.61881340949188 27.02911407209769 23.274168705151776 58-59 23.251028126295363 26.08996265228688 27.021093171737075 23.637916049680683 60-61 23.160051816458648 26.22269050907046 27.35934226407507 23.257915410395817 62-63 23.04379585422528 26.260919517665986 27.22897640919249 23.466308218916247 64-65 22.956596703477 26.167799244617413 27.18459842765697 23.691005624248614 66-67 22.856233063506608 26.470543261336832 27.02123113558245 23.651992539574103 68-69 22.73028775042867 26.197327686354637 27.266447383375052 23.805937179841642 70-71 22.834825459839873 26.268266203138324 27.160297904575813 23.736610432445993 72-73 22.992200275984512 26.279217969492922 26.80399355126935 23.92458820325321 74-75 22.884707656028574 26.530344038374988 26.68096182131594 23.903986484280495 76-77 22.950046307374343 26.388198376576277 26.733592290948344 23.928163025101036 78-79 22.977115767609305 26.400327974406757 26.794240280100723 23.828315977883218 80-81 22.80254874465895 26.78822714046074 26.641494135019162 23.767729979861148 82-83 22.965006833488932 26.51724324616967 26.635336349408462 23.882413570932936 84-85 22.90842959522685 26.297794644875545 26.636679490565157 24.157096269332452 86-87 22.81949298694343 26.173523092315953 26.846312829560347 24.160671091180276 88-89 22.922563572937317 26.227269402293917 26.675320628457815 24.174846396310947 90-91 22.84010057461764 26.55858421884402 26.72999004833665 23.871325158201692 92-93 22.875745548201095 26.078813206287073 26.745673836713372 24.299767408798463 94-95 22.70903598847952 26.09696857987239 26.72268897144305 24.471306460205042 96-97 22.832255930091776 26.144291925685316 26.710680292168696 24.31277185205421 98-99 22.82590366226243 26.118480438836645 26.45561788279173 24.599998016109193 100-101 22.44791964837276 26.15102552777877 26.73756694436103 24.663487879487437 102-103 22.667378943137397 25.82447206984167 26.73595174154697 24.77219724547396 104-105 22.62766301489604 25.929362364272645 26.734680593196607 24.708294027634707 106-107 22.603775448774478 26.040213780762087 26.650428443976377 24.70558232648706 108-109 22.63161092616826 26.05620646070086 26.38945492331462 24.922727689816256 110-111 22.462952144405993 26.35611032595252 26.496095641340016 24.684841888301474 112-113 22.40505340352133 26.165824308758857 26.66456438820184 24.76455789951798 114-115 22.25152192690618 26.309332418592923 26.36237771890744 25.07676793559346 116-117 22.17569443497358 26.271985670956866 26.535509965900744 25.016809928168808 118-119 22.10207286230973 26.481465371257734 26.218661319388755 25.197800447043782 120-121 22.096028631707714 26.574031024571276 26.345448496608043 24.984491847112963 122-123 22.053566723870905 26.627722324220947 26.226844940007872 25.091866011900272 124-125 22.040774310051443 26.708231426497264 26.10253688076611 25.14845738268518 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 58.0 1 57.5 2 106.0 3 161.0 4 121.0 5 99.0 6 232.0 7 553.0 8 935.0 9 1102.5 10 918.0 11 700.0 12 664.5 13 760.0 14 912.5 15 1029.5 16 1219.0 17 1442.5 18 1625.0 19 1794.0 20 1981.5 21 2279.5 22 2638.0 23 3212.5 24 4042.0 25 5260.0 26 6984.5 27 9440.5 28 12295.5 29 15907.5 30 20406.0 31 24701.5 32 29359.5 33 34736.0 34 40086.5 35 44583.0 36 50973.0 37 57962.0 38 62970.5 39 66302.5 40 68154.5 41 73446.0 42 79386.0 43 84386.5 44 90261.0 45 95144.5 46 96286.0 47 95966.0 48 97667.5 49 96298.5 50 94131.0 51 92358.0 52 91346.5 53 91258.5 54 90565.5 55 85472.5 56 76042.5 57 68366.0 58 62970.0 59 56501.5 60 47956.5 61 39685.0 62 31665.5 63 24486.5 64 19459.5 65 15476.0 66 11307.5 67 9369.5 68 6881.5 69 3992.0 70 3089.5 71 2476.5 72 2005.5 73 1534.0 74 1269.5 75 1106.5 76 677.0 77 307.0 78 150.5 79 98.0 80 57.5 81 26.5 82 14.5 83 7.5 84 4.5 85 3.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04500556060438873 2 0.006736369493586191 3 0.0010331855051512564 4 0.07827413387025918 5 0.00495929042472603 6 0.0 7 0.00652973239255594 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.0331855051512564E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.199113030907538E-4 28-29 7.438935637089046E-4 30-31 8.678758243270553E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0010538492152542816 42-43 8.265484041210051E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.95929042472603E-4 58-59 3.30619361648402E-4 60-61 2.6862823133932663E-4 62-63 3.5128307175142715E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.0663710103025127E-4 92-93 2.0663710103025127E-4 94-95 4.1327420206050254E-4 96-97 0.002954910544732593 98-99 0.008802740503888703 100-101 0.017956764079528835 102-103 0.009443315517082482 104-105 0.0040707508902959495 106-107 0.012666854293154402 108-109 0.013844685769026832 110-111 0.006674378363277117 112-113 0.004298051701429226 114-115 0.002934246834629568 116-117 0.0 118-119 0.0011365040556663819 120-121 2.479645212363015E-4 122-123 3.5128307175142715E-4 124-125 0.03409512166999146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2419701.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.31370377085405 #Duplication Level Percentage of deduplicated Percentage of total 1 81.46751793217662 52.39477815236747 2 10.898714002789086 14.018733277172727 3 3.431266807483383 6.620324310457512 4 1.475579030248869 3.795998105676394 5 0.7940336773137753 2.553362335342003 6 0.4634124458479238 1.788226245959419 7 0.2911860817574448 1.3109078783040797 8 0.192233725660828 0.9890610309534493 9 0.1476762803049181 0.8547847690960902 >10 0.7453795663082458 8.786103403701093 >50 0.06564015225570316 2.9477758258449267 >100 0.02675565065794154 3.142759102753635 >500 1.5116179882138426E-4 0.08555162132763788 >1k 4.5348539646415274E-4 0.7116339410435333 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2984 0.12332102189485394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2752 0.11373306040705031 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0663710103025126E-5 0.0 12-13 0.0 0.0 0.0 4.132742020605025E-5 0.0 14-15 0.0 0.0 0.0 4.132742020605025E-5 6.199113030907538E-5 16-17 0.0 0.0 0.0 4.132742020605025E-5 8.26548404121005E-5 18-19 0.0 0.0 0.0 4.132742020605025E-5 1.2398226061815075E-4 20-21 0.0 0.0 0.0 8.26548404121005E-5 1.2398226061815075E-4 22-23 0.0 0.0 0.0 1.4464597072117588E-4 1.2398226061815075E-4 24-25 0.0 0.0 0.0 5.165927525756281E-4 1.2398226061815075E-4 26-27 0.0 0.0 0.0 0.0010951766354603317 1.2398226061815075E-4 28-29 0.0 0.0 0.0 0.003636812978132422 1.2398226061815075E-4 30-31 0.0 0.0 0.0 0.01076579296367609 1.2398226061815075E-4 32-33 0.0 0.0 0.0 0.028763884463410973 1.65309680824201E-4 34-35 0.0 0.0 0.0 0.05823033507032481 1.65309680824201E-4 36-37 0.0 0.0 0.0 0.1074099651155246 1.65309680824201E-4 38-39 0.0 0.0 0.0 0.1838037013664085 1.65309680824201E-4 40-41 0.0 0.0 0.0 0.2638755780156309 1.65309680824201E-4 42-43 0.0 0.0 0.0 0.3447533393588712 1.65309680824201E-4 44-45 0.0 0.0 0.0 0.4394137953408293 1.65309680824201E-4 46-47 0.0 0.0 0.0 0.5555025186996244 1.65309680824201E-4 48-49 0.0 0.0 0.0 0.6803526551421023 1.65309680824201E-4 50-51 0.0 0.0 0.0 0.8151213724340322 1.65309680824201E-4 52-53 0.0 0.0 0.0 0.9502413727977135 1.8597339092722614E-4 54-55 0.0 0.0 0.0 1.092924291059102 2.0663710103025127E-4 56-57 0.0 0.0 0.0 1.2666441018952341 2.0663710103025127E-4 58-59 0.0 0.0 0.0 1.4577420929280105 2.0663710103025127E-4 60-61 0.0 0.0 0.0 1.665474370593722 2.0663710103025127E-4 62-63 0.0 0.0 0.0 1.8778146556124082 2.0663710103025127E-4 64-65 0.0 0.0 0.0 2.0867867558843014 2.0663710103025127E-4 66-67 0.0 0.0 0.0 2.322663006710333 2.479645212363015E-4 68-69 0.0 0.0 0.0 2.593915529232744 2.8929194144235176E-4 70-71 0.0 0.0 0.0 2.8913076450354813 2.8929194144235176E-4 72-73 0.0 0.0 0.0 3.1887204245483223 4.1327420206050254E-4 74-75 0.0 0.0 0.0 3.4914024501374343 4.1327420206050254E-4 76-77 0.0 0.0 0.0 3.8239228731153148 4.3393791216352767E-4 78-79 0.0 0.0 0.0 4.191530275848132 4.5460162226655275E-4 80-81 0.0 0.0 0.0 4.596311693056291 4.5460162226655275E-4 82-83 0.0 0.0 0.0 5.019380493705627 4.5460162226655275E-4 84-85 0.0 0.0 0.0 5.441560754820534 4.752653323695779E-4 86-87 0.0 0.0 0.0 5.867460483753984 4.95929042472603E-4 88-89 0.0 0.0 0.0 6.327207369836191 4.95929042472603E-4 90-91 0.0 0.0 0.0 6.842849591747079 4.95929042472603E-4 92-93 0.0 0.0 0.0 7.388598839278076 5.372564626786533E-4 94-95 0.0 0.0 0.0 7.9300706988177465 5.372564626786533E-4 96-97 0.0 0.0 0.0 8.47449746890215 5.372564626786533E-4 98-99 0.0 0.0 0.0 9.035475870779075 5.372564626786533E-4 100-101 0.0 0.0 0.0 9.659685225571259 5.372564626786533E-4 102-103 0.0 0.0 0.0 10.32404416909362 5.372564626786533E-4 104-105 0.0 0.0 0.0 10.984662981087332 5.372564626786533E-4 106-107 0.0 0.0 0.0 11.636003787244787 5.785838828847035E-4 108-109 0.0 0.0 0.0 12.304557463918062 6.40575013193779E-4 110-111 0.0 0.0 0.0 13.028634529638166 6.819024333998291E-4 112-113 0.0 0.0 0.0 13.793171139739993 7.025661435028543E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3595 0.0 62.324574 1 GTATCAA 5915 0.0 55.40874 1 TCAACGC 7790 0.0 41.588303 4 ATCAACG 7790 0.0 41.511856 3 CAACGCA 7960 0.0 40.663944 5 TATCAAC 8200 0.0 39.368523 2 AACGCAG 8255 0.0 39.209972 6 GTACATG 12255 0.0 36.274036 1 TACATGG 12580 0.0 35.03602 2 ACATGGG 12700 0.0 33.903484 3 ACGCAGA 10190 0.0 31.647528 7 CGCAGAG 10290 0.0 31.337383 8 CATGGGA 8320 0.0 29.991657 4 GCAGAGT 11870 0.0 26.865372 9 CATGGGG 5775 0.0 26.090254 4 ATGGGAG 3675 0.0 25.90516 5 GTGGTAT 1915 0.0 25.82734 1 AGTACAT 9190 0.0 24.368904 2 GAGTACA 9645 0.0 24.280672 1 GAGTACT 5855 0.0 23.472746 12-13 >>END_MODULE