FastQCFastQC Report
Thu 26 May 2016
SRR1364414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574076
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29050.042.8267141
ATCAACG28850.040.820613
CAACGCA29600.040.1920935
TCAACGC30550.038.9392284
GTACATG88250.038.043371
AACGCAG32550.036.548786
TACATGG91550.035.7975542
ACATGGG91600.034.6092263
TATCAAC35700.034.487542
GGTATCA22950.034.23781
ACGCAGA40900.029.2325447
CGCAGAG43700.027.495648
CATGGGG65000.027.4521564
CATGGGA40050.023.910684
GTATAGG7700.023.192371
GAGTACA73100.023.0453681
AGTACAT68450.022.9398522
GTACCGT2704.561116E-822.0307926
GTGTAAG11450.020.7954881
ATGGGGA37900.020.4035855
Produced by FastQC (version 0.11.5)