##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364414_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574076 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.813944498919223 31.0 31.0 34.0 27.0 34.0 2 30.870308413582194 31.0 31.0 34.0 27.0 34.0 3 30.792823133427294 31.0 31.0 34.0 27.0 34.0 4 34.40415473358207 37.0 35.0 37.0 30.0 37.0 5 34.55629709456908 37.0 35.0 37.0 32.0 37.0 6 34.625231733639566 37.0 35.0 37.0 31.0 37.0 7 34.53160823534348 37.0 35.0 37.0 31.0 37.0 8 34.390478758202946 37.0 35.0 37.0 30.0 37.0 9 35.51127122897692 39.0 35.0 39.0 29.0 39.0 10-11 35.745047737518234 39.0 35.0 39.0 30.0 39.0 12-13 35.79676862687815 39.0 35.0 39.0 30.0 39.0 14-15 36.961805712030255 40.0 37.0 41.0 30.5 41.0 16-17 37.09614051799558 40.0 37.0 41.0 31.0 41.0 18-19 37.057178381679485 40.0 37.0 41.0 31.0 41.0 20-21 37.00235890470989 40.0 37.0 41.0 30.0 41.0 22-23 36.894701632741224 40.0 37.0 41.0 30.0 41.0 24-25 36.93409538024518 40.0 37.0 41.0 30.0 41.0 26-27 36.933225942046775 40.0 37.0 41.0 30.0 41.0 28-29 36.87908826312821 40.0 36.5 41.0 30.0 41.0 30-31 36.65879057184014 40.0 36.0 41.0 30.0 41.0 32-33 36.7352735505867 40.0 36.0 41.0 30.0 41.0 34-35 36.68468335822253 40.0 36.0 41.0 30.0 41.0 36-37 36.56731347481582 40.0 36.0 41.0 30.0 41.0 38-39 36.43294817247043 40.0 36.0 41.0 29.5 41.0 40-41 36.39908573018046 40.0 36.0 41.0 29.0 41.0 42-43 36.261036581670474 40.0 35.0 41.0 29.0 41.0 44-45 36.23733254185191 40.0 35.0 41.0 29.0 41.0 46-47 36.08640770513381 39.0 35.0 41.0 29.0 41.0 48-49 35.912283281457114 39.0 35.0 41.0 28.0 41.0 50-51 35.772082875563896 39.0 35.0 41.0 28.0 41.0 52-53 35.646797141964726 39.0 35.0 41.0 28.0 41.0 54-55 35.52139019982316 39.0 34.5 40.0 28.0 41.0 56-57 35.471136633106404 38.5 34.5 40.0 28.0 41.0 58-59 35.44466519248071 38.5 34.5 40.0 28.0 41.0 60-61 35.25452473042754 38.0 34.0 40.0 28.0 41.0 62-63 34.99147053156162 37.5 34.0 40.0 28.0 41.0 64-65 34.68424436574522 37.0 34.0 40.0 27.5 41.0 66-67 34.368494753068674 36.5 34.0 39.5 27.0 41.0 68-69 34.06168679557247 36.0 33.5 39.0 26.0 41.0 70-71 33.71265086967129 36.0 33.0 39.0 26.0 41.0 72-73 33.37685406336099 35.0 33.0 38.5 26.0 40.0 74-75 33.021571235658925 35.0 33.0 37.0 26.0 39.5 76-77 32.679206441457055 35.0 33.0 37.0 26.0 39.0 78-79 32.3729153684662 35.0 32.5 36.0 25.5 39.0 80-81 32.06369858543415 35.0 32.0 36.0 25.0 37.5 82-83 31.790587768193326 35.0 32.0 35.5 25.0 37.0 84-85 31.468111858391126 35.0 32.0 35.0 23.5 37.0 86-87 31.284783938003386 35.0 32.0 35.0 23.5 36.0 88-89 31.15988222569963 35.0 32.0 35.0 23.0 36.0 90-91 31.04330583090787 35.0 32.0 35.0 23.0 36.0 92-93 30.829382854274698 35.0 32.0 35.0 20.5 35.5 94-95 30.761026480958606 35.0 31.5 35.0 21.0 35.0 96-97 30.69702798207978 34.5 31.5 35.0 20.0 35.0 98-99 30.556254360788103 34.0 31.0 35.0 19.5 35.0 100-101 30.42795084527419 34.0 31.0 35.0 19.0 35.0 102-103 29.37656716429507 33.5 29.5 34.5 18.0 35.0 104-105 30.34733279048482 34.0 31.0 35.0 19.0 35.0 106-107 30.605599834659117 35.0 32.0 35.0 19.5 35.0 108-109 30.599665277948283 35.0 32.0 35.0 19.0 35.0 110-111 30.49776424627711 35.0 32.0 35.0 18.5 35.0 112-113 30.385390330355435 35.0 32.0 35.0 17.5 35.0 114-115 30.28955982651639 35.0 32.0 35.0 15.0 35.0 116-117 30.174507473749806 35.0 32.0 35.0 8.5 35.0 118-119 29.91807623395735 35.0 31.0 35.0 2.0 35.0 120-121 29.794827930488452 35.0 31.0 35.0 2.0 35.0 122-123 29.561702335129187 35.0 31.0 35.0 2.0 35.0 124-125 28.377870156125926 34.0 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6107001585790393 1101 2 0.5835828221343426 1101 3 0.7516355746409396 1101 4 0.5301515674116715 1101 5 0.511660502822707 1101 6 0.5400979147063509 1101 7 0.6055098164545285 1101 8 0.6562462617951539 1101 9 1.058114684533649 1101 10-11 0.8138589486076384 1101 12-13 0.6921852183813471 1101 14-15 0.8988481492764322 1101 16-17 0.6867730883919521 1101 18-19 0.8140818536413477 1101 20-21 0.7863848328294125 1101 22-23 0.758644298138222 1101 24-25 0.785026997246149 1101 26-27 0.7592459824120823 1101 28-29 0.7156117865298128 1101 30-31 0.8499900989391875 1101 32-33 0.7667875226184577 1101 34-35 0.6677602924160055 1101 36-37 0.6667001378728301 1101 38-39 0.822096866988737 1101 40-41 0.7286860567716644 1101 42-43 0.7079214099694227 1101 44-45 0.5669120192323831 1101 46-47 0.6209474079422463 1101 48-49 0.5812057523771799 1101 50-51 0.555619537994005 1101 52-53 0.37359254255886043 1101 54-55 0.38586206367668296 1101 56-57 0.4706943812092135 1101 58-59 0.5432842022307227 1101 60-61 0.4951710921353012 1101 62-63 0.64822604504986 1101 64-65 0.6062914408475777 1101 66-67 0.559824848695726 1101 68-69 0.5744448606900434 1101 70-71 0.47284593655894014 1101 72-73 0.48488008060962073 1101 74-75 0.41812392978278723 1101 76-77 0.5011987232698303 1101 78-79 0.4346419334853806 1101 80-81 0.5062808666822889 1101 82-83 0.43288564054896383 1101 84-85 0.6139695506068605 1101 86-87 0.5644762129881364 1101 88-89 0.5563374057583275 1101 90-91 0.49022095726440185 1101 92-93 0.64185217094143 1101 94-95 0.5944889565017029 1101 96-97 0.5576816168787886 1101 98-99 0.5589557052304102 1101 100-101 0.48294887387368846 1101 102-103 0.3676793396508735 1101 104-105 0.4753510172323452 1101 106-107 0.3748071479209436 1101 108-109 0.38215129308485984 1101 110-111 0.4540714177583567 1101 112-113 0.35443102531338155 1101 114-115 0.4003634430549674 1101 116-117 0.4477049164581537 1101 118-119 0.4318611842295539 1101 120-121 0.2063663341691928 1101 122-123 0.2494391866798331 1101 124-125 -0.0713133335899947 1102 1 0.36003997458552206 1102 2 0.3058002776851332 1102 3 0.42820057501546316 1102 4 0.3128403786719076 1102 5 0.34415422103070625 1102 6 0.24609915404091964 1102 7 0.3465256622322812 1102 8 0.4107940068190885 1102 9 0.7276931064930281 1102 10-11 0.44179164320699726 1102 12-13 0.4548478810440031 1102 14-15 0.5561755446990659 1102 16-17 0.4184489819726025 1102 18-19 0.2928688038568765 1102 20-21 0.5527001277161361 1102 22-23 0.5399902699603842 1102 24-25 0.5755644734979057 1102 26-27 0.4560523125517477 1102 28-29 0.4531248097571208 1102 30-31 0.5834575800257227 1102 32-33 0.4028685015565827 1102 34-35 0.2862284204079373 1102 36-37 0.31644852571646 1102 38-39 0.4385752501337876 1102 40-41 0.30334302666672386 1102 42-43 0.39254004220710215 1102 44-45 0.2434634005456715 1102 46-47 0.40915057709779035 1102 48-49 0.3118002924955334 1102 50-51 0.2631515598117389 1102 52-53 0.18538801149718864 1102 54-55 0.12257564800931675 1102 56-57 0.25570660431667847 1102 58-59 0.3455367949595143 1102 60-61 0.29042757310607215 1102 62-63 0.2652321668178885 1102 64-65 0.33033589346345593 1102 66-67 0.3335434652714824 1102 68-69 0.2933644605620245 1102 70-71 0.34347915481121305 1102 72-73 0.3336002293297682 1102 74-75 0.2682393513267769 1102 76-77 0.326942153775164 1102 78-79 0.236035844403105 1102 80-81 0.32181544888354097 1102 82-83 0.2930621435826062 1102 84-85 0.3672316562499205 1102 86-87 0.3587759549069247 1102 88-89 0.3518383488783172 1102 90-91 0.26572911848684555 1102 92-93 0.31380984289910074 1102 94-95 0.26226898142458666 1102 96-97 0.23308627323582698 1102 98-99 0.30826487834910665 1102 100-101 0.24445868300254503 1102 102-103 0.1493208451019008 1102 104-105 0.15759788995540802 1102 106-107 0.08077638246160745 1102 108-109 0.1322953146574548 1102 110-111 0.0405803756958818 1102 112-113 0.1116208682175106 1102 114-115 -0.005351795993608022 1102 116-117 -0.029147446108492403 1102 118-119 0.13416416748491855 1102 120-121 0.012166011397443555 1102 122-123 0.09867413591478424 1102 124-125 -0.2804585332113838 1103 1 0.3367978924483559 1103 2 0.37264208872359816 1103 3 0.5837997481513852 1103 4 0.4657408281765143 1103 5 0.3938150541177947 1103 6 0.28669103112545 1103 7 0.28886448211797955 1103 8 0.41612550586574315 1103 9 0.9490174066819961 1103 10-11 0.6010573349359447 1103 12-13 0.5171020184628716 1103 14-15 0.6819316498013421 1103 16-17 0.5275548911545442 1103 18-19 0.6321631775151602 1103 20-21 0.6095590031001024 1103 22-23 0.6007453085410646 1103 24-25 0.3688505080662523 1103 26-27 0.49835982171175885 1103 28-29 0.5123754658146638 1103 30-31 0.7657410908191125 1103 32-33 0.674243583828769 1103 34-35 0.6203847692120377 1103 36-37 0.5700675237601871 1103 38-39 0.6020425803269163 1103 40-41 0.612694496737916 1103 42-43 0.5861573507762614 1103 44-45 0.5322513884466744 1103 46-47 0.4437996458320086 1103 48-49 0.36569599907750217 1103 50-51 0.3979599544782175 1103 52-53 0.2504991874041025 1103 54-55 0.19937436766155514 1103 56-57 0.2766032195619985 1103 58-59 0.3384453069759843 1103 60-61 0.38396487624803655 1103 62-63 0.34426718982148685 1103 64-65 0.34659353116052216 1103 66-67 0.21776696905910597 1103 68-69 0.25961899980186587 1103 70-71 0.3750583373533871 1103 72-73 0.24456103808580565 1103 74-75 0.43829524097213124 1103 76-77 0.37050822364635394 1103 78-79 0.23878371935067833 1103 80-81 0.295345720158366 1103 82-83 0.2969389658831467 1103 84-85 0.3214724331212082 1103 86-87 0.38773651709100676 1103 88-89 0.3719689276505598 1103 90-91 0.32427844690439755 1103 92-93 0.2938740429328952 1103 94-95 0.24134346898868486 1103 96-97 0.3182130262287757 1103 98-99 0.3006351068065136 1103 100-101 0.20798299219335092 1103 102-103 0.17196450099442018 1103 104-105 0.2408886602710112 1103 106-107 0.2193464672810599 1103 108-109 0.25035905938110403 1103 110-111 0.3178168258162657 1103 112-113 0.3089259279655501 1103 114-115 0.30976782728617636 1103 116-117 0.20408526890323841 1103 118-119 0.1985229727459057 1103 120-121 0.1089401586530876 1103 122-123 0.21487401184603527 1103 124-125 -0.024424891141251237 1104 1 0.5734316233337715 1104 2 0.5506873281867506 1104 3 0.7059338903815586 1104 4 0.5486746738385264 1104 5 0.5673743412159311 1104 6 0.4889207530081947 1104 7 0.617723784265003 1104 8 0.7149128015296924 1104 9 1.0674231682261208 1104 10-11 0.8157972442990911 1104 12-13 0.734820363823772 1104 14-15 0.7725392094694143 1104 16-17 0.6460259628121037 1104 18-19 0.6594638945035598 1104 20-21 0.7140274967066347 1104 22-23 0.7952495938843498 1104 24-25 0.8287638141782736 1104 26-27 0.749246731770441 1104 28-29 0.7757153929698575 1104 30-31 0.8370565941710169 1104 32-33 0.8781202870511606 1104 34-35 0.8168700852681212 1104 36-37 0.6853418608465134 1104 38-39 0.8430936206612571 1104 40-41 0.7923305726345475 1104 42-43 0.748307428930481 1104 44-45 0.6150189144380533 1104 46-47 0.6079844640955585 1104 48-49 0.7141253901924074 1104 50-51 0.6769235477111906 1104 52-53 0.5381106344019031 1104 54-55 0.5249044563320524 1104 56-57 0.5851161460934406 1104 58-59 0.6682549065243535 1104 60-61 0.6423715504673524 1104 62-63 0.6564035137555564 1104 64-65 0.6136805539704824 1104 66-67 0.6146245463525659 1104 68-69 0.6915190100822741 1104 70-71 0.7267709877236754 1104 72-73 0.622885797143411 1104 74-75 0.6505241399089883 1104 76-77 0.6732086782662705 1104 78-79 0.548699819889535 1104 80-81 0.717055301786516 1104 82-83 0.659522577569593 1104 84-85 0.6058775316939773 1104 86-87 0.564754163768292 1104 88-89 0.5634378629816297 1104 90-91 0.6099166183647853 1104 92-93 0.6341183912046162 1104 94-95 0.6151924663662811 1104 96-97 0.4917423821954259 1104 98-99 0.4690632878576828 1104 100-101 0.5160828434734412 1104 102-103 0.4678436058856619 1104 104-105 0.5131847254360196 1104 106-107 0.37532156714336296 1104 108-109 0.24810121377139538 1104 110-111 0.317138985031427 1104 112-113 0.3884148093513602 1104 114-115 0.41611134284434215 1104 116-117 0.46184198251371456 1104 118-119 0.46795244307896056 1104 120-121 0.26070695424794366 1104 122-123 0.34540790632078355 1104 124-125 0.09204162790789638 1105 1 0.3562636186936352 1105 2 0.3801791699510275 1105 3 0.4547434521790663 1105 4 0.30184801166231523 1105 5 0.27955908189794343 1105 6 0.3067257511762591 1105 7 0.37276711958597275 1105 8 0.43466506018089746 1105 9 0.8913842796597464 1105 10-11 0.4899945531276657 1105 12-13 0.5190452138504114 1105 14-15 0.19075994632172666 1105 16-17 0.4521474493422133 1105 18-19 0.4551441451795952 1105 20-21 0.7382348462632677 1105 22-23 0.7028503865828526 1105 24-25 0.6436635690102293 1105 26-27 0.6346819284501777 1105 28-29 0.5963869324973317 1105 30-31 0.7633494484405219 1105 32-33 0.6344864418393286 1105 34-35 0.565450735125566 1105 36-37 0.642869485023148 1105 38-39 0.7193346950178849 1105 40-41 0.6937617960821996 1105 42-43 0.7283267645405758 1105 44-45 0.631116437780662 1105 46-47 0.6545827333482919 1105 48-49 0.45847816947490827 1105 50-51 0.4316096266229863 1105 52-53 0.3291891944166849 1105 54-55 0.3109194009337557 1105 56-57 0.38535227300465635 1105 58-59 0.5078640793541922 1105 60-61 0.4869145845664846 1105 62-63 0.49707904138075065 1105 64-65 0.4747782945311414 1105 66-67 0.40218471863531136 1105 68-69 0.4596833727638838 1105 70-71 0.4756148302455898 1105 72-73 0.4321369215832078 1105 74-75 0.4768103494848077 1105 76-77 0.45219613844135864 1105 78-79 0.4855188429106789 1105 80-81 0.4760190063807386 1105 82-83 0.5547029512077053 1105 84-85 0.5827321797565261 1105 86-87 0.577324424836192 1105 88-89 0.5344837544271961 1105 90-91 0.5443479088360661 1105 92-93 0.4055672958169332 1105 94-95 0.4962110562584776 1105 96-97 0.4819967567042056 1105 98-99 0.47484859261460244 1105 100-101 0.3845324858582089 1105 102-103 0.4000936491050062 1105 104-105 0.4539352349372905 1105 106-107 0.4083895868926497 1105 108-109 0.48791519310479714 1105 110-111 0.45137775239140865 1105 112-113 0.36486395168608254 1105 114-115 0.3003613876015123 1105 116-117 0.25059749482215565 1105 118-119 0.08232155792759244 1105 120-121 0.13011402202343803 1105 122-123 0.18781646873586055 1105 124-125 0.009683565164813501 1106 1 0.40047444289489675 1106 2 0.3694701740563353 1106 3 0.47833360106575284 1106 4 0.3180508799178696 1106 5 0.34300070532412974 1106 6 0.24454860430027026 1106 7 0.33659328288349855 1106 8 0.5602270575572419 1106 9 0.9076570222855551 1106 10-11 0.4109682000713164 1106 12-13 0.6345672000393705 1106 14-15 0.7610274222916473 1106 16-17 0.5679846188512627 1106 18-19 0.5312461934531783 1106 20-21 0.7116541991055669 1106 22-23 0.6650165344843089 1106 24-25 0.6581936323644442 1106 26-27 0.6710477948951734 1106 28-29 0.6694118569971366 1106 30-31 0.6991125673379344 1106 32-33 0.6757295393277332 1106 34-35 0.6787859654648685 1106 36-37 0.5341586902377529 1106 38-39 0.7670209892911686 1106 40-41 0.7099248609018503 1106 42-43 0.6747827520784782 1106 44-45 0.44344080433986477 1106 46-47 0.538233858852827 1106 48-49 0.5772919247687511 1106 50-51 0.4877788254141322 1106 52-53 0.43746979391632124 1106 54-55 0.38512869600929633 1106 56-57 0.40643308976368075 1106 58-59 0.44064374980544585 1106 60-61 0.450300102616616 1106 62-63 0.6024704604462343 1106 64-65 0.6809212873140567 1106 66-67 0.5907388039245944 1106 68-69 0.5022240747405178 1106 70-71 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2216 78-79 -1.2807170083394652 2216 80-81 -1.2352421696136702 2216 82-83 -1.5199921219138162 2216 84-85 -1.512715636550844 2216 86-87 -1.5124606942217547 2216 88-89 -1.7537555199546233 2216 90-91 -1.5857432031654213 2216 92-93 -1.484079071956394 2216 94-95 -1.520991610319296 2216 96-97 -1.4223081389709264 2216 98-99 -1.453940895681857 2216 100-101 -1.6036544118971818 2216 102-103 -1.7686118139618614 2216 104-105 -1.695666969736191 2216 106-107 -1.3588228936415234 2216 108-109 -1.3235736548361956 2216 110-111 -1.3454368771594147 2216 112-113 -1.5172723858685444 2216 114-115 -1.4975150439409184 2216 116-117 -1.3833438853949076 2216 118-119 -1.1735138975196229 2216 120-121 -1.2120721118124038 2216 122-123 -1.1136160195630325 2216 124-125 -1.015781831002549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38657.0 3 7579.0 4 4915.0 5 4371.0 6 4621.0 7 5500.0 8 6253.0 9 6950.0 10 7509.0 11 8118.0 12 8805.0 13 9465.0 14 9350.0 15 8678.0 16 8163.0 17 7746.0 18 7366.0 19 7241.0 20 7362.0 21 8074.0 22 9740.0 23 12530.0 24 17762.0 25 24357.0 26 29523.0 27 34455.0 28 41465.0 29 49746.0 30 60476.0 31 73466.0 32 92829.0 33 123208.0 34 169247.0 35 247297.0 36 442582.0 37 716423.0 38 251763.0 39 484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08018051015972 17.68959904690138 12.054216815938272 25.176003627000625 2 16.208002274998172 19.53172954249849 38.66032288191299 25.59994530059035 3 19.010627502839856 23.587881632088564 29.00166894839158 28.399821916680008 4 12.791528879385364 15.524650973420522 36.47922157438027 35.20459857281384 5 13.908470060523465 37.63118953335278 34.52988159094467 13.930458815179078 6 33.96694728581585 35.58718294374279 16.969507433752774 13.476362336688592 7 29.9491662053359 31.492944067286306 20.65145731961967 17.90643240775813 8 27.00640119904477 34.84960853503566 19.706307067192583 18.437683198726987 9 26.71600506592698 15.636776143524239 19.224804394613955 38.42241439593483 10-11 25.441654240385787 25.95736108531746 27.74274373835886 20.858240935937893 12-13 25.714812958667927 23.130849290800807 27.698414548392986 23.455923202138283 14-15 22.888126601000437 24.925013884159394 24.657975704129388 27.52888381071078 16-17 23.529606013315355 28.035513307777986 25.469702850132148 22.965177828774515 18-19 23.497699932218957 27.07952684208921 26.446612375780898 22.976160849910936 20-21 23.877285018855602 26.606553329070888 26.452976981809996 23.06318467026352 22-23 23.86016061225287 26.20986126363406 26.322552871907074 23.607425252205996 24-25 23.63220743415548 26.509285166598385 26.285278689040503 23.573228710205633 26-27 23.51246396406897 26.81720166911439 26.113791951263103 23.556542415553537 28-29 23.517040890561244 26.75329973299092 26.31281731003649 23.416842066411345 30-31 23.719699957453173 26.4620662992227 26.242085610430383 23.57614813289375 32-33 23.424321165427457 26.580952675099763 26.277711700991308 23.717014458481472 34-35 23.894653806759123 26.567410495498038 25.96928610264034 23.5686495951025 36-37 23.923686900835587 26.406988044343287 25.9424677847605 23.726857270060624 38-39 23.474515824756768 26.53181656428657 26.30392825675157 23.689739354205095 40-41 23.71392069531314 26.25089135363182 26.31604199555212 23.719145955502928 42-43 23.584385402646333 26.220481663953333 26.28949836918168 23.905634564218655 44-45 23.49901109262319 26.393551337132532 26.2741287766802 23.83330879356408 46-47 23.76766821688682 26.73014786466271 25.963707676989078 23.53847624146139 48-49 23.713057103483433 26.38300443184604 26.213318383089128 23.6906200815814 50-51 23.517586397397334 26.5515089872563 26.303352243103866 23.627552372242498 52-53 23.708618173998367 26.480969104820524 26.05354055870115 23.756872162479958 54-55 23.565912133731903 26.289305659268976 26.164104891241426 23.9806773157577 56-57 23.434362350449124 26.678378130036872 26.280993918988095 23.60626560052591 58-59 23.586865853028378 26.293334048209875 26.203255718720946 23.9165443800408 60-61 23.360822931811136 26.35131722395721 26.541229346862373 23.74663049736928 62-63 23.365128160695235 26.474919878317227 26.508353694417252 23.651598266570282 64-65 23.417353038363967 26.360111591129353 26.24034985579055 23.98218551471612 66-67 23.329178539818024 26.53135483309464 26.19606377349019 23.94340285359715 68-69 23.24570563130003 26.28077296596773 26.586513794957046 23.887007607775196 70-71 23.267210662404523 26.191613488613058 26.42780427264641 24.11337157633601 72-73 23.38673314785894 26.323894550405914 26.184701334875566 24.104670966859583 74-75 23.37793557609239 26.60703770899227 26.020403306537798 23.994623408377542 76-77 23.49910245006559 26.444321114830643 26.1859052122777 23.870671222826072 78-79 23.487193573446504 26.409431506903868 26.337711089099408 23.765663830550217 80-81 23.54703327919003 26.543195845866236 26.245747652576 23.66402322236773 82-83 23.510615997306537 26.588416909205815 26.127182925192127 23.77378416829552 84-85 23.5934485491115 26.365958938133282 26.16566757344972 23.8749249393055 86-87 23.580229500795166 26.22256207373664 26.43276748833282 23.76444093713537 88-89 23.49590167995084 26.313816052639094 26.345657661326122 23.84462460608394 90-91 23.53335195706543 26.551484452615746 26.221424777671103 23.693738812647716 92-93 23.60103836806119 26.11292087542905 26.404507805729672 23.881532950780088 94-95 23.470368368060743 26.246430728400455 26.369940783941125 23.913260119597673 96-97 23.579146169636637 26.168711950217737 26.428439746939265 23.823702133206364 98-99 23.509232009479685 26.23572947929718 26.154200297211457 24.100838214011674 100-101 23.345799247305123 26.104968163062004 26.48119904336689 24.068033546265983 102-103 23.28471625662885 25.93685879989292 26.50030868910287 24.278116254375362 104-105 23.17742481303061 26.20013388675263 26.43802958796216 24.1844117122546 106-107 23.348963645973907 26.132019193057832 26.255856025259217 24.263161135709044 108-109 23.367073323289294 26.037160868034864 26.220785719336227 24.37498008933962 110-111 23.351732618212086 26.275305491909723 26.33558314207207 24.03737874780612 112-113 23.380839080852756 26.304730941494963 26.412292957203643 23.902137020448635 114-115 23.217898788085893 26.50620876984977 26.229374118621607 24.04651832344273 116-117 23.117911614224358 26.424894120417058 26.486631713113297 23.97056255224529 118-119 23.194222930547582 26.477151348481865 26.143415640143203 24.185210080827346 120-121 23.181110513335987 26.694240744313575 26.254283434394893 23.870365307955545 122-123 23.23848320097241 26.780266364290256 26.167048717393726 23.8142017173436 124-125 23.25151924138785 26.909516841130536 26.0344197833538 23.804544134127813 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 248.0 1 244.5 2 197.0 3 144.5 4 126.0 5 120.0 6 119.0 7 113.5 8 117.5 9 105.5 10 105.5 11 120.5 12 110.5 13 113.0 14 132.5 15 166.0 16 195.0 17 240.5 18 333.0 19 436.0 20 550.5 21 771.0 22 1131.0 23 1711.0 24 2463.5 25 3586.0 26 5585.5 27 8765.5 28 12298.0 29 15934.5 30 20244.5 31 25170.0 32 31181.0 33 36296.5 34 39625.0 35 44185.0 36 52252.0 37 59245.5 38 63556.5 39 67775.5 40 71738.0 41 76874.0 42 80844.0 43 84407.5 44 90198.5 45 96732.5 46 99581.0 47 99454.5 48 99891.0 49 99865.0 50 99408.0 51 98616.5 52 99645.0 53 101778.5 54 105160.5 55 101260.0 56 89919.5 57 82120.5 58 76210.5 59 66747.5 60 54832.0 61 45761.5 62 37925.0 63 29751.0 64 22570.5 65 16955.0 66 12519.5 67 10011.0 68 7569.5 69 5124.0 70 3953.0 71 3002.5 72 2270.5 73 1673.0 74 1217.5 75 938.5 76 696.0 77 422.5 78 239.5 79 156.5 80 101.0 81 66.5 82 44.0 83 29.5 84 16.5 85 11.0 86 10.5 87 9.0 88 8.5 89 9.0 90 7.5 91 9.0 92 8.0 93 2.0 94 2.5 95 3.5 96 1.0 97 0.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08756540210933943 2 3.107911343721009E-4 3 0.0 4 0.002330933507790757 5 0.0012820134292849162 6 2.3309335077907565E-4 7 3.884889179651261E-5 8 0.0010489200785058406 9 6.215822687442018E-4 10-11 0.0089352451131979 12-13 0.0017870490226395803 14-15 0.003865464733753005 16-17 0.0021366890488081934 18-19 0.013499989899288132 20-21 0.01699639016097427 22-23 0.009226611801671745 24-25 0.010353229663770612 26-27 0.009945316299907228 28-29 0.007517260562625191 30-31 0.01713236128226206 32-33 0.0019618690357238867 34-35 0.001146042307997122 36-37 8.741000654215338E-4 38-39 3.107911343721009E-4 40-41 0.0016122290095552736 42-43 0.002389206845485526 44-45 8.935245113197901E-4 46-47 0.0012820134292849162 48-49 0.007964022818285087 50-51 0.0038071913960582363 52-53 0.007594958346218216 54-55 0.008158267277267648 56-57 0.009498554044247334 58-59 0.007964022818285087 60-61 0.009867618516314203 62-63 0.012994954305933469 64-65 0.008371936182148468 66-67 0.013286320994407312 68-69 0.01272301206335788 70-71 0.00378776695015998 72-73 0.01225682536179973 74-75 0.012684163171561368 76-77 0.00483668702866582 78-79 0.007478411670828677 80-81 0.013616536574677672 82-83 0.016957541269177756 84-85 0.012645314279764855 86-87 0.01565610339399458 88-89 0.015520132272706788 90-91 0.011615818647157272 92-93 0.008915820667299644 94-95 0.007633807238014729 96-97 0.008838122883706619 98-99 0.005866182661273404 100-101 0.010003589637601997 102-103 0.011227329729192145 104-105 0.010061862975296767 106-107 0.00483668702866582 108-109 0.003030213560127984 110-111 0.005963304890764686 112-113 0.008235965060860674 114-115 0.013111500981323007 116-117 0.010858265257125275 118-119 0.2166408451032526 120-121 0.02503811076285238 122-123 0.026533793097018112 124-125 0.2337926308314129 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2574076.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.51035869393307 #Duplication Level Percentage of deduplicated Percentage of total 1 86.65912865984133 64.57002760548473 2 8.52729824967006 12.707441025461277 3 2.3041836028066522 5.150566402254079 4 0.9244838499633538 2.7553449307007076 5 0.460516547330154 1.715662656303069 6 0.27301479376549254 1.2205458127330204 7 0.1754682963195128 0.9151943988726164 8 0.11522708785577941 0.6868489317913171 9 0.08605512617428533 0.5770798486827902 >10 0.42079224804404664 5.723215634422041 >50 0.03901515892278018 2.012234540299397 >100 0.014586669575762035 1.8694765154994724 >500 2.297097308307099E-4 0.09636169749541534 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.769778359302522E-5 2 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 3 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 4 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 5 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 6 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 7 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 8 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 9 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 10-11 3.884889179651261E-5 0.0 0.0 0.0 7.769778359302522E-5 12-13 3.884889179651261E-5 0.0 0.0 0.0 1.1654667538953784E-4 14-15 3.884889179651261E-5 0.0 0.0 0.0 1.1654667538953784E-4 16-17 3.884889179651261E-5 0.0 0.0 0.0 1.1654667538953784E-4 18-19 5.827333769476892E-5 0.0 0.0 0.0 1.1654667538953784E-4 20-21 7.769778359302522E-5 0.0 0.0 1.9424445898256306E-5 1.5539556718605044E-4 22-23 7.769778359302522E-5 0.0 0.0 5.827333769476892E-5 1.5539556718605044E-4 24-25 7.769778359302522E-5 0.0 0.0 7.769778359302522E-5 1.5539556718605044E-4 26-27 7.769778359302522E-5 0.0 0.0 3.1079113437210094E-4 2.136689048808194E-4 28-29 9.712222949128152E-5 0.0 0.0 0.0022143868324012187 2.3309335077907568E-4 30-31 1.1654667538953784E-4 0.0 0.0 0.009556827381942103 2.52517796677332E-4 32-33 1.1654667538953784E-4 0.0 0.0 0.025989908611866938 3.107911343721009E-4 34-35 1.3597112128779413E-4 0.0 0.0 0.050251041538789065 3.107911343721009E-4 36-37 1.5539556718605044E-4 0.0 0.0 0.09057619122356916 3.107911343721009E-4 38-39 1.5539556718605044E-4 0.0 0.0 0.15308405812415796 3.107911343721009E-4 40-41 1.7482001308430676E-4 0.0 0.0 0.22190486994168004 3.107911343721009E-4 42-43 1.9424445898256308E-4 0.0 0.0 0.28971561057249284 3.302155802703572E-4 44-45 1.9424445898256308E-4 0.0 0.0 0.3711817366697797 3.6906447206686986E-4 46-47 2.719422425755883E-4 0.0 0.0 0.47415072437643646 3.8848891796512615E-4 48-49 2.719422425755883E-4 0.0 0.0 0.585142008239073 3.8848891796512615E-4 50-51 2.719422425755883E-4 0.0 0.0 0.6999210590518694 3.8848891796512615E-4 52-53 2.719422425755883E-4 0.0 3.884889179651261E-5 0.8113979540619625 4.2733780976163873E-4 54-55 3.107911343721009E-4 0.0 3.884889179651261E-5 0.931868367522948 4.2733780976163873E-4 56-57 3.107911343721009E-4 0.0 3.884889179651261E-5 1.0770661006124138 4.2733780976163873E-4 58-59 3.107911343721009E-4 0.0 3.884889179651261E-5 1.235006270211136 4.2733780976163873E-4 60-61 3.107911343721009E-4 0.0 3.884889179651261E-5 1.4098262832954427 4.2733780976163873E-4 62-63 3.107911343721009E-4 0.0 3.884889179651261E-5 1.588492336667604 4.2733780976163873E-4 64-65 3.107911343721009E-4 0.0 3.884889179651261E-5 1.7650993987745505 4.2733780976163873E-4 66-67 3.107911343721009E-4 0.0 3.884889179651261E-5 1.9726496031974192 4.2733780976163873E-4 68-69 3.496400261686135E-4 0.0 3.884889179651261E-5 2.2063839606911375 4.2733780976163873E-4 70-71 3.496400261686135E-4 0.0 3.884889179651261E-5 2.4559492415919344 4.2733780976163873E-4 72-73 3.6906447206686986E-4 0.0 3.884889179651261E-5 2.7120799852063424 4.46762255659895E-4 74-75 3.8848891796512615E-4 0.0 3.884889179651261E-5 2.969667562263119 4.6618670155815136E-4 76-77 3.8848891796512615E-4 0.0 3.884889179651261E-5 3.253439292390745 4.6618670155815136E-4 78-79 3.8848891796512615E-4 0.0 3.884889179651261E-5 3.570543371679779 4.6618670155815136E-4 80-81 3.8848891796512615E-4 0.0 3.884889179651261E-5 3.914336639633018 4.6618670155815136E-4 82-83 3.8848891796512615E-4 0.0 3.884889179651261E-5 4.2709500418791055 4.6618670155815136E-4 84-85 3.8848891796512615E-4 0.0 3.884889179651261E-5 4.630360564334541 4.6618670155815136E-4 86-87 3.8848891796512615E-4 0.0 3.884889179651261E-5 5.00389265895801 4.6618670155815136E-4 88-89 3.8848891796512615E-4 0.0 3.884889179651261E-5 5.409552787097195 4.6618670155815136E-4 90-91 3.8848891796512615E-4 0.0 3.884889179651261E-5 5.856043100514515 4.6618670155815136E-4 92-93 3.8848891796512615E-4 0.0 3.884889179651261E-5 6.310633407871407 5.050355933546639E-4 94-95 3.8848891796512615E-4 0.0 3.884889179651261E-5 6.778276942871928 5.050355933546639E-4 96-97 3.8848891796512615E-4 0.0 3.884889179651261E-5 7.247610404665597 5.827333769476892E-4 98-99 4.2733780976163873E-4 0.0 3.884889179651261E-5 7.744118666270926 6.215822687442018E-4 100-101 4.2733780976163873E-4 0.0 3.884889179651261E-5 8.289459984864472 6.215822687442018E-4 102-103 4.6618670155815136E-4 0.0 3.884889179651261E-5 8.865122863505196 6.410067146424581E-4 104-105 5.438844851511766E-4 0.0 3.884889179651261E-5 9.43676488184498 6.604311605407145E-4 106-107 5.438844851511766E-4 0.0 3.884889179651261E-5 10.006173088906465 6.604311605407145E-4 108-109 5.438844851511766E-4 0.0 3.884889179651261E-5 10.605164727070996 6.604311605407145E-4 110-111 5.438844851511766E-4 0.0 3.884889179651261E-5 11.241470725806074 6.798556064389707E-4 112-113 5.438844851511766E-4 0.0 3.884889179651261E-5 11.904873826569222 6.99280052337227E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2905 0.0 42.826714 1 ATCAACG 2885 0.0 40.82061 3 CAACGCA 2960 0.0 40.192093 5 TCAACGC 3055 0.0 38.939228 4 GTACATG 8825 0.0 38.04337 1 AACGCAG 3255 0.0 36.54878 6 TACATGG 9155 0.0 35.797554 2 ACATGGG 9160 0.0 34.609226 3 TATCAAC 3570 0.0 34.48754 2 GGTATCA 2295 0.0 34.2378 1 ACGCAGA 4090 0.0 29.232544 7 CGCAGAG 4370 0.0 27.49564 8 CATGGGG 6500 0.0 27.452156 4 CATGGGA 4005 0.0 23.91068 4 GTATAGG 770 0.0 23.19237 1 GAGTACA 7310 0.0 23.045368 1 AGTACAT 6845 0.0 22.939852 2 GTACCGT 270 4.561116E-8 22.030792 6 GTGTAAG 1145 0.0 20.795488 1 ATGGGGA 3790 0.0 20.403585 5 >>END_MODULE