FastQCFastQC Report
Thu 26 May 2016
SRR1364414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574076
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30450.055.1808741
GTATCAA47150.050.169011
ATCAACG55050.042.6281743
TCAACGC57600.040.6367954
CAACGCA57550.040.6325955
TATCAAC60750.039.022182
AACGCAG60650.038.4553956
GTACATG85400.034.7454721
ACGCAGA70450.033.1904837
TACATGG89200.032.8529972
CGCAGAG72000.032.3088078
ACATGGG89850.031.7523063
CATGGGG59900.027.8407294
GCAGAGT93250.024.7548089
CATGGGA38750.023.5163064
CAGAGTA83450.023.34864210-11
ATGGGGA37450.022.879055
AGTACAT65600.022.336032
GAGTACA72550.021.9280781
GTGGTAT16850.021.5703241
Produced by FastQC (version 0.11.5)