FastQCFastQC Report
Thu 26 May 2016
SRR1364413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692095
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.047.0609321
GTATCAA33650.046.8717161
CAACGCA36250.040.6922075
ATCAACG37000.040.5043683
TCAACGC36750.040.297434
AACGCAG38400.038.4138686
TATCAAC41900.037.0449942
GTACATG88550.032.128421
ACGCAGA47650.030.5823427
TACATGG91050.030.3068712
CGCAGAG47900.030.0502038
ACATGGG89750.029.5531273
CATGGGG56900.025.1906914
TAAGGTG16700.021.7260595
CTAGACG1653.2259323E-421.6273174
GAGTACA67600.021.042691
AGAGTAC54300.020.97666710-11
GCAGAGT66150.020.7706329
GTAAGGT15000.020.6180424
GGTAAGG15050.019.7577083
Produced by FastQC (version 0.11.5)