FastQCFastQC Report
Thu 26 May 2016
SRR1364413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692095
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.067.383211
GTATCAA51750.051.697231
TCAACGC66800.039.583274
ATCAACG66750.039.3452643
CAACGCA67300.039.2578055
AACGCAG70250.037.6064646
TATCAAC69700.037.4257662
GTACATG90150.031.7253781
ACGCAGA84250.031.3573197
CGCAGAG85800.030.787418
TACATGG93700.029.6829242
ACATGGG93350.028.7080273
CATGGGG59250.024.4243554
GCAGAGT107100.023.7200669
AGAGTAC93800.023.69000810-11
CAGAGTA96800.022.46412310-11
GAGTACT54400.022.25578712-13
TAAGGTG16450.020.6189735
GTACTTT58000.020.51537514-15
CATGGGA43450.020.2850994
Produced by FastQC (version 0.11.5)