##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364413_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2692095 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95127549362114 33.0 31.0 34.0 30.0 34.0 2 31.921406562547013 33.0 31.0 34.0 30.0 34.0 3 32.12279135766011 34.0 31.0 34.0 30.0 34.0 4 35.79473941298505 37.0 35.0 37.0 35.0 37.0 5 35.73906752919195 37.0 35.0 37.0 33.0 37.0 6 35.795765751208634 37.0 35.0 37.0 33.0 37.0 7 35.70669942925491 37.0 35.0 37.0 33.0 37.0 8 35.72236529542977 37.0 35.0 37.0 33.0 37.0 9 37.37456664790804 39.0 37.0 39.0 34.0 39.0 10-11 37.32251796463349 39.0 37.0 39.0 34.0 39.0 12-13 37.30464285249963 39.0 37.0 39.0 33.5 39.0 14-15 38.61848263155646 40.0 38.0 41.0 34.0 41.0 16-17 38.626748870303615 40.0 38.0 41.0 34.0 41.0 18-19 38.66931739035955 40.0 38.0 41.0 34.0 41.0 20-21 38.67387239306191 40.0 38.0 41.0 34.0 41.0 22-23 38.6460006054764 40.0 38.0 41.0 34.0 41.0 24-25 38.591951621320945 40.0 38.0 41.0 34.0 41.0 26-27 38.480607668005774 40.0 38.0 41.0 34.0 41.0 28-29 38.4106075379955 40.0 38.0 41.0 34.0 41.0 30-31 38.303471459959624 40.0 38.0 41.0 33.5 41.0 32-33 38.196868238305115 40.0 38.0 41.0 33.0 41.0 34-35 38.10790629602596 40.0 38.0 41.0 33.0 41.0 36-37 38.01404426663992 40.0 37.0 41.0 33.0 41.0 38-39 37.92657242779322 40.0 37.0 41.0 33.0 41.0 40-41 37.81452474745505 40.0 37.0 41.0 33.0 41.0 42-43 37.69887801136289 40.0 37.0 41.0 32.5 41.0 44-45 37.55145732227132 40.0 37.0 41.0 32.0 41.0 46-47 37.38800952418098 40.0 37.0 41.0 31.5 41.0 48-49 37.20286561209764 40.0 36.0 41.0 31.0 41.0 50-51 36.993809096632916 40.0 36.0 41.0 31.0 41.0 52-53 36.86839078858658 39.0 36.0 41.0 31.0 41.0 54-55 36.713462935000436 39.0 35.0 41.0 30.5 41.0 56-57 36.50485866954918 39.0 35.0 41.0 30.0 41.0 58-59 36.28971470174715 39.0 35.0 40.5 30.0 41.0 60-61 36.051856639531664 38.0 35.0 40.0 29.5 41.0 62-63 36.094956901595225 38.0 35.0 40.0 30.0 41.0 64-65 35.88544850757495 37.5 35.0 40.0 30.0 41.0 66-67 35.60896179369599 37.0 35.0 40.0 30.0 41.0 68-69 35.28496041187253 37.0 34.0 39.5 29.0 41.0 70-71 34.94348899277328 36.0 34.0 39.0 29.0 41.0 72-73 34.5927214678531 36.0 34.0 39.0 29.0 40.5 74-75 34.25282707333879 35.0 34.0 37.5 29.0 40.0 76-77 33.93137370709429 35.0 34.0 37.0 29.0 39.0 78-79 33.60476989110711 35.0 34.0 37.0 29.0 39.0 80-81 33.32238256822289 35.0 33.0 36.0 28.0 38.5 82-83 33.05727881074033 35.0 33.0 36.0 28.0 37.0 84-85 32.82948484358836 35.0 33.0 35.5 28.0 37.0 86-87 32.6489345658307 35.0 33.0 35.0 27.0 36.5 88-89 32.4718386609685 35.0 33.0 35.0 27.0 36.0 90-91 32.35500158798259 35.0 33.0 35.0 27.0 36.0 92-93 32.238092266431906 35.0 33.0 35.0 27.0 36.0 94-95 32.13126208399035 35.0 33.0 35.0 27.0 35.0 96-97 32.07654317548229 35.0 33.0 35.0 26.5 35.0 98-99 32.00569147819821 35.0 33.0 35.0 26.0 35.0 100-101 31.998541470490455 35.0 33.0 35.0 26.5 35.0 102-103 30.88017714828043 33.5 30.5 34.5 24.5 35.0 104-105 31.85107193468284 35.0 32.5 35.0 26.0 35.0 106-107 32.043836677383226 35.0 33.0 35.0 27.0 35.0 108-109 32.05343923598536 35.0 33.0 35.0 27.0 35.0 110-111 31.99884736608478 35.0 33.0 35.0 27.0 35.0 112-113 31.944673757798295 35.0 33.0 35.0 27.0 35.0 114-115 31.843887567117804 35.0 33.0 35.0 26.0 35.0 116-117 31.767513219258607 35.0 33.0 35.0 25.0 35.0 118-119 31.672282738907803 35.0 33.0 35.0 25.0 35.0 120-121 31.557327100269493 35.0 33.0 35.0 25.0 35.0 122-123 31.383581745815064 35.0 33.0 35.0 25.0 35.0 124-125 30.369215239432485 34.5 31.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3265123099963141 1101 2 0.49689428954069825 1101 3 0.34367867719127787 1101 4 0.23601803992607984 1101 5 0.23232738869796776 1101 6 0.21684127825675859 1101 7 0.26953685554225615 1101 8 0.2355647537211567 1101 9 0.3103708060171826 1101 10-11 0.341790623101879 1101 12-13 0.306400816776069 1101 14-15 0.34867899108215994 1101 16-17 0.3410762976463815 1101 18-19 0.19328497030578973 1101 20-21 0.2666752359076838 1101 22-23 0.3144422688791053 1101 24-25 0.36644701165880633 1101 26-27 0.280400722186954 1101 28-29 0.279176852289595 1101 30-31 0.2778795924329813 1101 32-33 0.21168828844857046 1101 34-35 0.22448424452406357 1101 36-37 0.21369041419740853 1101 38-39 0.23170534065488368 1101 40-41 0.19724107889508957 1101 42-43 0.16479871211738129 1101 44-45 0.249872747155095 1101 46-47 0.19817300700438523 1101 48-49 0.09391000283964956 1101 50-51 0.09590709341983228 1101 52-53 0.11010611330499387 1101 54-55 0.11218201694457264 1101 56-57 0.15500282210058458 1101 58-59 0.04387452864202146 1101 60-61 -0.02503393236824536 1101 62-63 0.1809355006503779 1101 64-65 0.23836112698587186 1101 66-67 0.19155673552283758 1101 68-69 0.23194716874036203 1101 70-71 0.15019611010274758 1101 72-73 0.22179571562132594 1101 74-75 0.16895511700138854 1101 76-77 0.16285335456380778 1101 78-79 0.09986979269978491 1101 80-81 0.004149073790351565 1101 82-83 0.07631602398198112 1101 84-85 -0.016952616624742234 1101 86-87 0.16992993101004572 1101 88-89 0.18387583149095832 1101 90-91 0.08060912406325826 1101 92-93 0.09130392615295335 1101 94-95 0.2479841899540034 1101 96-97 0.05112683913247196 1101 98-99 0.09587640848495127 1101 100-101 0.10778619026443792 1101 102-103 0.1270435902657958 1101 104-105 0.1867519614063724 1101 106-107 0.16190442484669632 1101 108-109 0.14610542540029314 1101 110-111 0.116508814493681 1101 112-113 0.1385515673448161 1101 114-115 0.25097098996511846 1101 116-117 0.22253438674950488 1101 118-119 0.19134539620678126 1101 120-121 0.21227297454275984 1101 122-123 0.18005887459720782 1101 124-125 -0.053717206353621094 1102 1 0.4234604200205787 1102 2 0.4973040512026543 1102 3 0.3743548829679071 1102 4 0.24438261651463478 1102 5 0.27633827525937704 1102 6 0.3303363947666256 1102 7 0.3803839782756384 1102 8 0.3071673896338325 1102 9 0.36291621438306265 1102 10-11 0.43581593257344053 1102 12-13 0.41747637520696657 1102 14-15 0.44797914070617395 1102 16-17 0.5036968528759047 1102 18-19 0.4195270011828498 1102 20-21 0.4471801723620743 1102 22-23 0.4367100274702338 1102 24-25 0.36603136811960724 1102 26-27 0.3990728755416697 1102 28-29 0.4002597819437739 1102 30-31 0.4449519353646991 1102 32-33 0.42133166175058534 1102 34-35 0.3869946581825445 1102 36-37 0.3293292424224106 1102 38-39 0.28426831257977625 1102 40-41 0.3570555136018214 1102 42-43 0.42396817573065704 1102 44-45 0.39041421252932906 1102 46-47 0.33450934051459313 1102 48-49 0.28413284008433237 1102 50-51 0.3585701458963513 1102 52-53 0.29710456016111664 1102 54-55 0.2329261504696447 1102 56-57 0.20941527509070568 1102 58-59 0.1886568548648384 1102 60-61 0.1341273951041515 1102 62-63 0.254454397453145 1102 64-65 0.3906641308797205 1102 66-67 0.3188179343161721 1102 68-69 0.2730844436573321 1102 70-71 0.26038938694744473 1102 72-73 0.27607490989496597 1102 74-75 0.3434147968149688 1102 76-77 0.38047879093069525 1102 78-79 0.26189822071529534 1102 80-81 0.21835596310459238 1102 82-83 0.2240879852975084 1102 84-85 0.17904523919845872 1102 86-87 0.19611749843960524 1102 88-89 0.2705812678447259 1102 90-91 0.2151878979386197 1102 92-93 0.1702436430940324 1102 94-95 0.21723072640374852 1102 96-97 0.12570805768015703 1102 98-99 0.10961365589114891 1102 100-101 0.035655279702783815 1102 102-103 0.08240205703384618 1102 104-105 0.04044130461571527 1102 106-107 -0.03262411068405413 1102 108-109 -0.004042349242070031 1102 110-111 0.06433998305909583 1102 112-113 0.04487544987842185 1102 114-115 -0.0026897536420520396 1102 116-117 0.061984361739217064 1102 118-119 0.005705444132864557 1102 120-121 -0.035411891217481895 1102 122-123 -0.09675635752792999 1102 124-125 -0.1704623746513647 1103 1 0.38573610154764637 1103 2 0.4872327393843463 1103 3 0.34600892037695985 1103 4 0.18743364964109333 1103 5 0.19449852954088698 1103 6 0.19685951773691812 1103 7 0.2894843754826866 1103 8 0.3302858315126471 1103 9 0.34000996128325056 1103 10-11 0.40350054112395384 1103 12-13 0.3375440642900216 1103 14-15 0.4122765457064119 1103 16-17 0.48795951843274565 1103 18-19 0.3900720071991799 1103 20-21 0.3672622014388338 1103 22-23 0.30865253595563047 1103 24-25 0.35240837410142944 1103 26-27 0.3166925228425086 1103 28-29 0.3591717106621388 1103 30-31 0.36696371787982685 1103 32-33 0.3690689616914966 1103 34-35 0.345462694013122 1103 36-37 0.3325572504786791 1103 38-39 0.34271640180256924 1103 40-41 0.3055166058564964 1103 42-43 0.1692682158395158 1103 44-45 0.2743110465174823 1103 46-47 0.27328392875191554 1103 48-49 0.26441648084700375 1103 50-51 0.2962516600271812 1103 52-53 0.2631912836989727 1103 54-55 0.20139185739898835 1103 56-57 0.14561685874355135 1103 58-59 -0.008607387013121581 1103 60-61 0.05444371595773134 1103 62-63 0.25575426936357815 1103 64-65 0.3246132573261278 1103 66-67 0.19800623676281504 1103 68-69 0.2895949517459542 1103 70-71 0.24792335009043143 1103 72-73 0.24829144164350225 1103 74-75 0.20624407757264152 1103 76-77 0.14377212682295237 1103 78-79 0.1449923385145695 1103 80-81 0.17065893374749663 1103 82-83 0.1299484064836136 1103 84-85 0.146188701670809 1103 86-87 0.1372192627979274 1103 88-89 0.18062222847335363 1103 90-91 0.09779731316876905 1103 92-93 0.030374255638186298 1103 94-95 0.21242526513035642 1103 96-97 0.19766970412707252 1103 98-99 0.1354120053902257 1103 100-101 0.15419511253942986 1103 102-103 0.24349156231484415 1103 104-105 0.252679842324504 1103 106-107 0.1666742539097399 1103 108-109 0.1484116226127341 1103 110-111 0.1874973477779669 1103 112-113 0.20011795750125927 1103 114-115 0.2142337570471753 1103 116-117 0.20185202408304193 1103 118-119 0.19483308732794313 1103 120-121 0.09852812448103876 1103 122-123 0.16328999407577527 1103 124-125 0.018994603211137218 1104 1 0.37115605401487173 1104 2 0.5257517720446572 1104 3 0.37254580606803245 1104 4 0.2589678632431145 1104 5 0.28699321794923094 1104 6 0.297883818540285 1104 7 0.2970135838057004 1104 8 0.2536018723021982 1104 9 0.3517791014788614 1104 10-11 0.3921199943667375 1104 12-13 0.40003173574907436 1104 14-15 0.4438646572998053 1104 16-17 0.4349717752835005 1104 18-19 0.41500216357069775 1104 20-21 0.41689897112213714 1104 22-23 0.3976637869712363 1104 24-25 0.39735269471736245 1104 26-27 0.37710715285593466 1104 28-29 0.45192643895630624 1104 30-31 0.32622478749751593 1104 32-33 0.33715074934079325 1104 34-35 0.32201532982258385 1104 36-37 0.3071694717015916 1104 38-39 0.28227548538465186 1104 40-41 0.30519432584763706 1104 42-43 0.29191199812725444 1104 44-45 0.271767891193079 1104 46-47 0.2645184990669591 1104 48-49 0.19907613708165428 1104 50-51 0.1705045613076095 1104 52-53 0.151441206577708 1104 54-55 0.22057213434038658 1104 56-57 0.2426305276738816 1104 58-59 0.007542512837851234 1104 60-61 0.033246337646410495 1104 62-63 0.3032948612204365 1104 64-65 0.38371074295549334 1104 66-67 0.3087994670360601 1104 68-69 0.3078799702333299 1104 70-71 0.32778117540097185 1104 72-73 0.28082195342458505 1104 74-75 0.2106216872043234 1104 76-77 0.2924562315862147 1104 78-79 0.37241774812627426 1104 80-81 0.31840596077687167 1104 82-83 0.330260150206378 1104 84-85 0.24425594490043068 1104 86-87 0.3288993985313624 1104 88-89 0.4125617849500216 1104 90-91 0.2790478834316801 1104 92-93 0.3535455039599853 1104 94-95 0.3534543778664485 1104 96-97 0.3423121655773045 1104 98-99 0.30861817552625936 1104 100-101 0.18684342434398005 1104 102-103 0.29628112552604335 1104 104-105 0.31798314615623724 1104 106-107 0.3230024885495766 1104 108-109 0.22947563543876015 1104 110-111 0.40149855130425394 1104 112-113 0.25658582508729566 1104 114-115 0.3121717406844269 1104 116-117 0.19328537175440985 1104 118-119 0.1593588784183737 1104 120-121 0.2028167730991548 1104 122-123 0.06079718481948504 1104 124-125 0.023413977811955533 1105 1 0.32344923559915273 1105 2 0.4803540385211065 1105 3 0.29241952892372325 1105 4 0.2050956850266985 1105 5 0.2069869364753245 1105 6 0.16002121424829596 1105 7 0.18963642299642203 1105 8 0.1267022365921946 1105 9 0.1986637388390804 1105 10-11 0.26748824355670564 1105 12-13 0.2468265427090941 1105 14-15 0.2778375609022419 1105 16-17 0.3327384030014855 1105 18-19 0.29584535384967836 1105 20-21 0.32488397321596096 1105 22-23 0.38505864104230625 1105 24-25 0.43568559997002154 1105 26-27 0.3503005969667825 1105 28-29 0.3721432363095687 1105 30-31 0.3354836401171042 1105 32-33 0.32249181429904183 1105 34-35 0.30860254205477844 1105 36-37 0.26585231183275226 1105 38-39 0.2700940949608821 1105 40-41 0.3315294384883032 1105 42-43 0.37801005610490535 1105 44-45 0.35643476236253235 1105 46-47 0.34373610289760137 1105 48-49 0.16252636527305953 1105 50-51 0.14473831908403412 1105 52-53 0.160017459057471 1105 54-55 0.22833503354225115 1105 56-57 -0.03422837383118349 1105 58-59 0.0035807149345075118 1105 60-61 0.040193831888387876 1105 62-63 0.2707641680580082 1105 64-65 0.27238292986660895 1105 66-67 0.325861697988465 1105 68-69 0.3593352670773413 1105 70-71 0.3017894145122 1105 72-73 0.3152558866031896 1105 74-75 0.3444048823097461 1105 76-77 0.2978618036439542 1105 78-79 0.35812772768486667 1105 80-81 0.36008382851813536 1105 82-83 0.30684142032394135 1105 84-85 0.21380770894768375 1105 86-87 0.19269832393797515 1105 88-89 0.3312148124784926 1105 90-91 0.1710416325666415 1105 92-93 0.1359012496454639 1105 94-95 0.033792399681260576 1105 96-97 0.12920418012050305 1105 98-99 0.17249889095775117 1105 100-101 0.2070786646547198 1105 102-103 0.20331913775894606 1105 104-105 0.18523250159539373 1105 106-107 0.18847858079852386 1105 108-109 0.13208301271047418 1105 110-111 0.1768740867378007 1105 112-113 0.12945978349825893 1105 114-115 0.13591390436828732 1105 116-117 0.04951780492059399 1105 118-119 -0.017728890984102463 1105 120-121 -0.02637623272806522 1105 122-123 0.005536239706081858 1105 124-125 -0.04244351657198564 1106 1 0.2703658511895064 1106 2 0.4016500768562068 1106 3 0.21359368059252404 1106 4 0.18002011529359407 1106 5 0.08821186264682979 1106 6 0.12725807834814873 1106 7 0.1055990490620573 1106 8 0.08798929013875068 1106 9 0.1514483267924973 1106 10-11 0.22841399019960562 1106 12-13 0.20623480035660435 1106 14-15 0.29480157299339993 1106 16-17 0.27098104104683784 1106 18-19 0.222634127739461 1106 20-21 0.26195040493156796 1106 22-23 0.2647982911230997 1106 24-25 0.2194812900575016 1106 26-27 0.21157877019908256 1106 28-29 0.22394036814219476 1106 30-31 0.23537028551744754 1106 32-33 0.20738117603080752 1106 34-35 0.23636205617970063 1106 36-37 0.21776279826502787 1106 38-39 0.14136697482938843 1106 40-41 0.14061748245107708 1106 42-43 0.1520652603173076 1106 44-45 0.19346097147825958 1106 46-47 0.23968319711946862 1106 48-49 0.27403002883799843 1106 50-51 0.30952038572469576 1106 52-53 0.14591691300520182 1106 54-55 0.1011140843585423 1106 56-57 0.1871282595894428 1106 58-59 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36-37 -1.1044044765707284 2216 38-39 -1.19201938327911 2216 40-41 -1.0838692759880075 2216 42-43 -1.2433240583885308 2216 44-45 -1.182335796218311 2216 46-47 -1.109112468302932 2216 48-49 -1.2570267707013656 2216 50-51 -1.1450744036487919 2216 52-53 -0.9141728511397673 2216 54-55 -1.0104353709263023 2216 56-57 -1.2581172484804597 2216 58-59 -1.264419074499422 2216 60-61 -1.210966755399518 2216 62-63 -1.2393661201027868 2216 64-65 -1.4540312370444681 2216 66-67 -1.3811781194387507 2216 68-69 -1.2646401294617249 2216 70-71 -1.2146475139744979 2216 72-73 -1.1220301000789163 2216 74-75 -1.078023583627214 2216 76-77 -1.07404410803899 2216 78-79 -1.2162998343429052 2216 80-81 -1.3016019556441876 2216 82-83 -1.193462951468895 2216 84-85 -1.137834828058736 2216 86-87 -1.1131445113649683 2216 88-89 -1.0185991009746367 2216 90-91 -0.8992784716586968 2216 92-93 -0.9577103331754557 2216 94-95 -1.042443183518614 2216 96-97 -0.9507762914177071 2216 98-99 -1.0107737680926334 2216 100-101 -1.1400866261405938 2216 102-103 -1.1561690131140736 2216 104-105 -1.1428444802563433 2216 106-107 -1.170119409317529 2216 108-109 -1.0175161087520834 2216 110-111 -1.1670021231248668 2216 112-113 -0.947199932974037 2216 114-115 -0.9680760063049831 2216 116-117 -0.9529475863234929 2216 118-119 -1.1660390252791828 2216 120-121 -1.2908035368205084 2216 122-123 -1.2293281074028357 2216 124-125 -1.234092909142408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 101.0 3 16.0 4 44.0 5 119.0 6 354.0 7 876.0 8 1604.0 9 2807.0 10 4173.0 11 5473.0 12 7036.0 13 8877.0 14 11337.0 15 9193.0 16 6110.0 17 5719.0 18 5981.0 19 6240.0 20 6637.0 21 7006.0 22 7689.0 23 8843.0 24 11622.0 25 16229.0 26 21777.0 27 29197.0 28 37724.0 29 48674.0 30 60974.0 31 76021.0 32 95468.0 33 121745.0 34 162588.0 35 236868.0 36 447593.0 37 853287.0 38 365285.0 39 808.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.507324878872495 19.679847311337266 13.74145938670351 25.071368423086724 2 15.629989442534622 21.074067937772618 38.60079779073335 24.695144828959403 3 18.216299824893632 25.220443092659277 30.490758440134353 26.07249864231274 4 13.01369710235874 17.477932363586614 37.30103438328094 32.207336150773706 5 13.123167890766076 38.36661018882125 35.095402485446215 13.41481943496646 6 31.949689739775156 37.54340021433122 17.300392445288892 13.206517600604734 7 27.736747964761538 32.72388551772046 22.028607568133822 17.51075894938418 8 26.178422381082388 34.96540798151626 20.449984120174065 18.40618551722729 9 26.34372115397116 15.415837851190245 20.078971953070006 38.161469041768584 10-11 24.47809234072349 26.208491899431486 28.81744514959539 20.49597061024964 12-13 25.304363330417388 23.686719822294535 28.539743211142255 22.46917363614583 14-15 22.51434291880487 25.29687845339782 25.872898244675614 26.315880383121694 16-17 22.432937916381107 28.316162691138313 26.813411116621072 22.437488275859508 18-19 22.36576194911579 27.12477375870257 28.35766230172255 22.151801990459095 20-21 22.979761115413833 26.537064999563537 28.30563185920259 22.177542025820042 22-23 22.862157539017012 26.379158239215183 28.10411593944493 22.654568282322877 24-25 22.77711224901053 26.69818487089051 27.991954221526356 22.5327486585726 26-27 22.478892722767256 26.905672600490107 27.98899810221164 22.626436574531002 28-29 22.626983596327005 26.856779887670502 27.960926124037915 22.55531039196458 30-31 22.622365556408678 26.606816966973827 27.956908364805315 22.81390911181218 32-33 22.54688263229938 26.70706271509735 27.866438591505872 22.879616061097398 34-35 23.103698049288752 26.621757404549246 27.688937426056658 22.585607120105344 36-37 23.189616265399252 26.277583071919825 27.67383766174671 22.858963000934214 38-39 22.786974456696367 26.675470219290183 27.571092402014045 22.96646292199941 40-41 23.0813306129954 26.374895874639105 27.77448930979628 22.769284202569214 42-43 22.92576009634199 26.330065286034777 27.524329267760407 23.219845349862826 44-45 22.837269858604543 26.613975361196392 27.624229456984246 22.92452532321482 46-47 23.353266387936976 26.71624268761739 26.953232882426676 22.977258042018956 48-49 23.09792187868556 26.70459251995193 27.007014982755067 23.190470618607442 50-51 23.12636557267317 26.802054460208296 27.14138139307171 22.93019857404682 52-53 23.30027357875558 26.602070134969235 27.11033600225846 22.987320284016725 54-55 22.957399348834272 26.646273627045108 27.164401702020175 23.231925322100448 56-57 23.008911522388015 26.815322670065285 27.04889701314209 23.12686879440462 58-59 22.894829511390054 26.49922773640961 27.28283940409036 23.323103348109978 60-61 22.748612183955995 26.768445105558342 27.30397707802805 23.17896563245761 62-63 22.73401471350273 26.68349996378272 27.562558388758156 23.019926933956395 64-65 22.592349081291708 26.68601962412173 27.299426654705723 23.422204639880835 66-67 22.588950241354784 26.851466980177147 27.15751115766717 23.402071620800903 68-69 22.815725299441514 26.533071826960043 27.44578107384769 23.205421799750752 70-71 22.831549406688843 26.368868854925253 27.202030388972158 23.59755134941375 72-73 22.712590008896417 26.58039556553539 27.016654315690943 23.69036010987725 74-75 22.706108068251677 26.812612482100374 26.950367650472955 23.53091179917499 76-77 22.89484583567816 26.586784641700977 27.07755484111816 23.440814681502694 78-79 22.950174492356325 26.542358274875145 27.069865662244457 23.437601570524073 80-81 23.10202648866403 26.7391381061961 26.75191625852728 23.40691914661258 82-83 23.05702436206746 26.704722530222746 26.82756366324368 23.41068944446611 84-85 22.889311112720762 26.679649120851977 26.930048902434724 23.50099086399254 86-87 22.95827227493829 26.538978007834046 27.037567396395744 23.465182320831918 88-89 22.908831226238302 26.728291535031268 26.757543845963834 23.6053333927666 90-91 22.818687863089245 26.79078834488972 26.815713222015837 23.574810570005194 92-93 22.699189607180593 26.489553931992234 27.075752867800272 23.735503593026905 94-95 22.608924019058847 26.348593264026405 27.156610698852894 23.88587201806185 96-97 22.780202959983225 26.164646211183957 27.19590224176484 23.85924858706798 98-99 22.695438437075747 26.288430100991828 26.9151548664553 24.10097659547713 100-101 22.600343658570566 26.155259369340083 27.10567036641434 24.13872660567501 102-103 22.42548162919743 26.02768427547864 27.187925938006273 24.358908157317654 104-105 22.30556005450247 26.287580033789155 26.93230801920365 24.47455189250472 106-107 22.577378395030426 26.24324318802089 26.700313040932706 24.47906537601597 108-109 22.448421197322936 26.049789078129326 26.802420725511794 24.699368999035944 110-111 22.421130858128137 26.244157729553883 26.89533069716445 24.439380715153526 112-113 22.43346035853462 26.33920204993675 27.07897200783585 24.14836558369278 114-115 22.304407527508623 26.603429135635903 26.678707193614308 24.41345614324116 116-117 22.224535580010286 26.38505074766135 26.94589658721118 24.44451708511718 118-119 22.130456836104404 26.550883418543457 26.6479834016234 24.67067634372874 120-121 22.08662314528155 26.657780127050152 26.789276644714054 24.466320082954244 122-123 22.02873754498367 26.698384302444794 26.777560763243997 24.49531738932754 124-125 21.911719734383357 26.747247651367562 26.73786538359355 24.60316723065553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 28.0 2 71.0 3 120.0 4 84.5 5 68.0 6 152.5 7 401.0 8 723.5 9 830.5 10 638.0 11 440.0 12 409.0 13 428.5 14 502.5 15 612.0 16 710.0 17 800.0 18 898.5 19 1050.5 20 1241.5 21 1518.0 22 1914.0 23 2477.0 24 3338.5 25 4831.5 26 7206.0 27 10481.5 28 14287.5 29 19322.5 30 24820.5 31 31744.0 32 41441.5 33 47617.5 34 51290.0 35 56702.0 36 64952.0 37 73673.5 38 80297.0 39 85698.0 40 88589.0 41 91426.0 42 93861.5 43 95621.5 44 98737.0 45 102699.5 46 104307.5 47 103732.0 48 102009.5 49 100461.0 50 99192.5 51 96296.5 52 95097.5 53 96573.5 54 99030.5 55 95186.0 56 85323.0 57 75485.0 58 67791.5 59 59045.0 60 46614.5 61 38020.0 62 32647.0 63 24839.0 64 17791.5 65 13166.5 66 9176.5 67 7214.0 68 5602.0 69 3752.0 70 2696.5 71 1893.5 72 1398.5 73 991.0 74 688.0 75 521.0 76 340.5 77 199.0 78 127.0 79 66.5 80 26.5 81 8.0 82 6.0 83 6.0 84 2.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0419375987845897 2 0.005980472457324128 3 6.314784582267714E-4 4 0.08108926319464951 5 0.00683482566551329 6 0.0 7 0.005571868749059747 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 9.286447915099579E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.200410832455764E-4 28-29 5.571868749059747E-4 30-31 6.686242498871696E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0010215092706609536 42-43 6.871971457173688E-4 44-45 0.0 46-47 1.8572895830199156E-5 48-49 0.0 50-51 3.714579166039831E-5 52-53 0.0 54-55 0.0 56-57 6.871971457173688E-4 58-59 3.343121249435848E-4 60-61 2.0430185413219072E-4 62-63 3.7145791660398315E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.0430185413219072E-4 92-93 2.0430185413219072E-4 94-95 4.457494999247798E-4 96-97 0.0032131109786244544 98-99 0.009045000269306989 100-101 0.017458522080387208 102-103 0.008989281581816392 104-105 0.00397459970766262 106-107 0.010196519810779337 108-109 0.012629569164535427 110-111 0.006816252769683091 112-113 0.004067464186813616 114-115 0.002804507270360073 116-117 1.8572895830199156E-5 118-119 9.100718956797587E-4 120-121 3.157392291133857E-4 122-123 2.600205416227882E-4 124-125 0.031499631328017766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2692095.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.851840531934755 #Duplication Level Percentage of deduplicated Percentage of total 1 79.22330859505003 46.624375238481406 2 11.491288383634277 13.525669429202372 3 3.938091859420332 6.952918623321475 4 1.82277917941024 4.290956383663292 5 0.971684200010088 2.859270179319715 6 0.5948023980622903 2.100312952726457 7 0.3804230146473671 1.567201621489331 8 0.26777995191931764 1.2607474422403868 9 0.19054522719285802 1.0092543592387822 >10 0.983606477393436 10.672006923448711 >50 0.08929747077692886 3.638091897312152 >100 0.044885881091991445 4.553042933836141 >500 8.374229949524105E-4 0.29087348665372875 >1k 6.699383959619284E-4 0.6552785290660754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 1.1143737498119494E-4 6 0.0 0.0 0.0 0.0 1.4858316664159325E-4 7 0.0 0.0 0.0 0.0 1.4858316664159325E-4 8 0.0 0.0 0.0 0.0 1.4858316664159325E-4 9 0.0 0.0 0.0 0.0 1.4858316664159325E-4 10-11 0.0 0.0 0.0 0.0 1.4858316664159325E-4 12-13 0.0 0.0 0.0 0.0 6.871971457173688E-4 14-15 0.0 0.0 0.0 0.0 8.35780312358962E-4 16-17 0.0 0.0 0.0 0.0 9.286447915099579E-4 18-19 0.0 0.0 0.0 0.0 0.0010215092706609536 20-21 0.0 0.0 0.0 0.0 0.0012072382289629452 22-23 0.0 0.0 0.0 1.1143737498119494E-4 0.0013558213956045384 24-25 0.0 0.0 0.0 2.4144764579258905E-4 0.0013743942914347376 26-27 0.0 0.0 0.0 5.386139790757755E-4 0.0014672587705857334 28-29 0.0 0.0 0.0 0.002173028812133301 0.00154155035390653 30-31 0.0 0.0 0.0 0.008506386290231214 0.0015786961455669284 32-33 0.0 0.0 0.0 0.023680442183503923 0.0018201437913595174 34-35 0.0 0.0 0.0 0.04596791717974291 0.0018758624788501148 36-37 0.0 0.0 0.0 0.08472955077736856 0.001894435374680314 38-39 0.0 0.0 0.0 0.14393994268404348 0.0019687269580011104 40-41 0.0 0.0 0.0 0.2096508481312881 0.0020058727496615088 42-43 0.0 0.0 0.0 0.2752874619952119 0.002024445645491708 44-45 0.0 0.0 0.0 0.3563581522940312 0.002117310124642704 46-47 0.0 0.0 0.0 0.4544601880691432 0.002228747499623899 48-49 0.0 0.0 0.0 0.5580226552183336 0.002358757770435293 50-51 0.0 0.0 0.0 0.6713358926783788 0.002451622249586289 52-53 0.0 0.0 0.0 0.7827546947637435 0.0025630596245674836 54-55 0.0 0.0 0.0 0.9087160742841541 0.0026373512078882804 56-57 0.0 0.0 0.0 1.0549219102594818 0.0026559241037184796 58-59 0.0 3.714579166039831E-5 0.0 1.216728978732177 0.002693069895378878 60-61 0.0 3.714579166039831E-5 0.0 1.390849877140294 0.002748788582869475 62-63 0.0 3.714579166039831E-5 0.0 1.5635035167778253 0.0028973717495110684 64-65 0.0 3.714579166039831E-5 0.0 1.74579648935123 0.0030459549161526616 66-67 0.0 3.714579166039831E-5 0.0 1.9598491137942755 0.0031388193953036576 68-69 0.0 3.714579166039831E-5 0.0 2.1994208971080145 0.003175965186964056 70-71 0.0 3.714579166039831E-5 0.0 2.4577327323144242 0.0032688296661150516 72-73 0.0 3.714579166039831E-5 0.0 2.720130604603478 0.0035659959993982384 74-75 0.0 3.714579166039831E-5 0.0 2.9976096683066533 0.003733152061870031 76-77 0.0 3.714579166039831E-5 0.0 3.3007193282555036 0.0037702978535304288 78-79 0.0 3.714579166039831E-5 0.0 3.6383374286568637 0.003863162332681425 80-81 0.0 3.714579166039831E-5 0.0 3.9989302012001806 0.004030318395153217 82-83 0.0 3.714579166039831E-5 0.0 4.377928713511224 0.004346057624266602 84-85 0.0 3.714579166039831E-5 0.0 4.773345665736164 0.004346057624266602 86-87 0.0 3.714579166039831E-5 0.0 5.174297340918504 0.004494640790908196 88-89 0.0 3.714579166039831E-5 0.0 5.605857148429012 0.00464322395754979 90-91 0.0 3.714579166039831E-5 0.0 6.081843322765355 0.004661796853379989 92-93 0.0 3.714579166039831E-5 0.0 6.575473748140389 0.0047175155408705855 94-95 0.0 3.714579166039831E-5 0.0 7.08295955380475 0.004754661332530984 96-97 0.0 3.714579166039831E-5 0.0 7.613791489527673 0.0051632650407953656 98-99 0.0 3.714579166039831E-5 0.0 8.16665087970521 0.005348993999097358 100-101 0.0 3.714579166039831E-5 0.0 8.757194675522223 0.005348993999097358 102-103 0.0 3.714579166039831E-5 0.0 9.382748379979162 0.005460431374078553 104-105 0.0 3.714579166039831E-5 0.0 10.02505483647494 0.005534722957399349 106-107 0.0 3.714579166039831E-5 0.0 10.666878397679131 0.005534722957399349 108-109 0.0 3.714579166039831E-5 0.0 11.344231908606494 0.005571868749059747 110-111 0.0 3.714579166039831E-5 0.0 12.05832260748599 0.005664733228210744 112-113 0.0 3.714579166039831E-5 0.0 12.790168994779158 0.005683306124040942 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2865 0.0 67.38321 1 GTATCAA 5175 0.0 51.69723 1 TCAACGC 6680 0.0 39.58327 4 ATCAACG 6675 0.0 39.345264 3 CAACGCA 6730 0.0 39.257805 5 AACGCAG 7025 0.0 37.606464 6 TATCAAC 6970 0.0 37.425766 2 GTACATG 9015 0.0 31.725378 1 ACGCAGA 8425 0.0 31.357319 7 CGCAGAG 8580 0.0 30.78741 8 TACATGG 9370 0.0 29.682924 2 ACATGGG 9335 0.0 28.708027 3 CATGGGG 5925 0.0 24.424355 4 GCAGAGT 10710 0.0 23.720066 9 AGAGTAC 9380 0.0 23.690008 10-11 CAGAGTA 9680 0.0 22.464123 10-11 GAGTACT 5440 0.0 22.255787 12-13 TAAGGTG 1645 0.0 20.618973 5 GTACTTT 5800 0.0 20.515375 14-15 CATGGGA 4345 0.0 20.285099 4 >>END_MODULE