FastQCFastQC Report
Thu 26 May 2016
SRR1364412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820498
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.043.4495741
GTATCAA31950.042.296291
ATCAACG32250.040.571083
CAACGCA32900.039.5936665
TCAACGC35000.037.2160644
AACGCAG35950.036.3999946
GTACATG91200.033.2906761
TATCAAC40950.032.822992
TACATGG92950.031.9282232
ACATGGG93350.030.3897863
ACGCAGA44150.029.5041587
CATGGGG66550.027.973754
CGCAGAG46550.027.7274468
TAGTCCG1552.1119091E-423.0248625
GTGTAAG15600.022.5151421
GAGTACA75400.020.4491711
AGTACAT71200.019.8801922
GTATAGA8450.019.7264351
GCAGAGT68100.018.9535319
CAGAGTA58850.018.44564610-11
Produced by FastQC (version 0.11.5)