##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364412_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2820498 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.636588290436652 31.0 31.0 34.0 27.0 34.0 2 30.721989166452165 31.0 31.0 34.0 27.0 34.0 3 30.69483261466592 31.0 31.0 34.0 27.0 34.0 4 34.32295502425458 37.0 35.0 37.0 30.0 37.0 5 34.46732421012176 37.0 35.0 37.0 31.0 37.0 6 34.53890979536238 37.0 35.0 37.0 31.0 37.0 7 34.43240271753428 37.0 35.0 37.0 30.0 37.0 8 34.29892841618749 37.0 35.0 37.0 30.0 37.0 9 35.47998261299955 39.0 35.0 39.0 29.0 39.0 10-11 35.62998715120521 39.0 35.0 39.0 29.5 39.0 12-13 35.69077198423824 39.0 35.0 39.0 30.0 39.0 14-15 36.82960260918462 40.0 36.5 41.0 30.0 41.0 16-17 36.93484820765694 40.0 37.0 41.0 30.0 41.0 18-19 36.90333426933825 40.0 36.5 41.0 30.0 41.0 20-21 36.83659073681314 40.0 37.0 41.0 30.0 41.0 22-23 36.727425085924544 40.0 36.5 41.0 30.0 41.0 24-25 36.7568833588962 40.0 36.0 41.0 30.0 41.0 26-27 36.7386145992658 40.0 36.0 41.0 30.0 41.0 28-29 36.6786563933036 40.0 36.0 41.0 30.0 41.0 30-31 36.46615526761586 40.0 36.0 41.0 29.0 41.0 32-33 36.53220973742935 40.0 36.0 41.0 30.0 41.0 34-35 36.479210763489284 40.0 36.0 41.0 30.0 41.0 36-37 36.36766929102591 40.0 36.0 41.0 29.0 41.0 38-39 36.23475109714667 40.0 35.5 41.0 29.0 41.0 40-41 36.20606112821211 40.0 35.0 41.0 29.0 41.0 42-43 36.083123973142335 39.5 35.0 41.0 28.0 41.0 44-45 36.06562085844415 39.0 35.0 41.0 28.5 41.0 46-47 35.93098417371684 39.0 35.0 41.0 28.0 41.0 48-49 35.76081865684712 39.0 35.0 41.0 28.0 41.0 50-51 35.61860157319735 39.0 35.0 41.0 27.5 41.0 52-53 35.48236463915238 39.0 34.5 40.0 27.0 41.0 54-55 35.342884838067604 38.5 34.0 40.0 27.0 41.0 56-57 35.29250348697286 38.5 34.0 40.0 27.5 41.0 58-59 35.27634499297642 38.0 34.0 40.0 28.0 41.0 60-61 35.09359942818608 38.0 34.0 40.0 27.5 41.0 62-63 34.83232269620471 37.5 34.0 40.0 27.0 41.0 64-65 34.53614858085345 37.0 34.0 40.0 26.5 41.0 66-67 34.23394414745198 36.5 34.0 40.0 26.0 41.0 68-69 33.93824707551646 36.0 33.0 39.0 26.0 41.0 70-71 33.60093891220629 36.0 33.0 39.0 26.0 41.0 72-73 33.26176866638445 35.0 33.0 38.5 26.0 40.0 74-75 32.90189711178664 35.0 33.0 37.0 26.0 39.5 76-77 32.55950580358504 35.0 32.5 37.0 25.0 39.0 78-79 32.2443423111805 35.0 32.0 36.5 25.0 39.0 80-81 31.935383928653735 35.0 32.0 36.0 24.5 38.0 82-83 31.654027409344025 35.0 32.0 36.0 24.0 37.0 84-85 31.338047749014535 35.0 32.0 35.0 23.0 37.0 86-87 31.15426743787799 35.0 32.0 35.0 23.0 36.0 88-89 31.017200331289015 35.0 32.0 35.0 23.0 36.0 90-91 30.89666417065355 35.0 32.0 35.0 21.5 36.0 92-93 30.678670397922637 34.5 31.0 35.0 20.0 35.5 94-95 30.61315714458936 34.0 31.0 35.0 20.0 35.0 96-97 30.54871621961795 34.0 31.0 35.0 20.0 35.0 98-99 30.418342966383953 34.0 31.0 35.0 19.0 35.0 100-101 30.30817341476576 34.0 31.0 35.0 18.5 35.0 102-103 29.282925036642467 33.5 29.5 34.5 17.5 35.0 104-105 30.245102106081976 34.0 31.0 35.0 19.0 35.0 106-107 30.497854456907966 35.0 32.0 35.0 19.0 35.0 108-109 30.498089699053146 35.0 32.0 35.0 19.0 35.0 110-111 30.40744772731624 35.0 32.0 35.0 18.0 35.0 112-113 30.305887293662323 35.0 32.0 35.0 16.0 35.0 114-115 30.21721465500064 35.0 32.0 35.0 11.5 35.0 116-117 30.108329096492888 35.0 31.0 35.0 6.0 35.0 118-119 29.855644818751866 35.0 31.0 35.0 2.0 35.0 120-121 29.7442542061721 35.0 31.0 35.0 2.0 35.0 122-123 29.52849354972065 34.0 31.0 35.0 2.0 35.0 124-125 28.336269339669805 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6269216634070638 1101 2 0.6253242502642316 1101 3 0.80245713249057 1101 4 0.5598388704508039 1101 5 0.6134752946141404 1101 6 0.5663908648228144 1101 7 0.719656266552505 1101 8 0.6937195389767652 1101 9 1.1938354468964434 1101 10-11 0.9568215396726245 1101 12-13 0.8047851854786714 1101 14-15 1.055892457221134 1101 16-17 0.782223171105187 1101 18-19 0.9116909548437562 1101 20-21 0.9741634809323969 1101 22-23 0.9918115328210035 1101 24-25 1.0313013140513902 1101 26-27 0.9894184660949179 1101 28-29 0.9237152079637241 1101 30-31 1.1252714743764543 1101 32-33 0.955385207036386 1101 34-35 0.8096829159879846 1101 36-37 0.8112866533329353 1101 38-39 0.9849737393006208 1101 40-41 0.8141074183607842 1101 42-43 0.8912473650731059 1101 44-45 0.8344395600371399 1101 46-47 0.78184028882972 1101 48-49 0.7830078115153754 1101 50-51 0.6289005083230776 1101 52-53 0.5546388109211193 1101 54-55 0.5924339143451576 1101 56-57 0.6606515329020013 1101 58-59 0.8168263243300373 1101 60-61 0.6719697649069047 1101 62-63 0.7137902586664708 1101 64-65 0.6148748330981562 1101 66-67 0.7060530212663423 1101 68-69 0.6589950696217173 1101 70-71 0.578171605616788 1101 72-73 0.6282600925494677 1101 74-75 0.6258722826548961 1101 76-77 0.6344104274515274 1101 78-79 0.548236866765663 1101 80-81 0.6890697896686184 1101 82-83 0.6197794769367526 1101 84-85 0.7405795291930168 1101 86-87 0.6958536931957333 1101 88-89 0.5657523520446723 1101 90-91 0.5764760065165859 1101 92-93 0.6610654582954112 1101 94-95 0.6536279817697981 1101 96-97 0.5711207522766486 1101 98-99 0.5409550190066525 1101 100-101 0.5442422666945923 1101 102-103 0.5052542634123149 1101 104-105 0.499552468434473 1101 106-107 0.45642992386404657 1101 108-109 0.4854077131715542 1101 110-111 0.5795709710276107 1101 112-113 0.4139718775838226 1101 114-115 0.42523942657375713 1101 116-117 0.4154582161189637 1101 118-119 0.3038837332376012 1101 120-121 0.20736901339549618 1101 122-123 0.2572004540876165 1101 124-125 -0.14694347579721523 1102 1 0.14962654507541373 1102 2 0.042728678713690016 1102 3 0.15894769161494438 1102 4 0.04045427714947181 1102 5 0.02334767899799317 1102 6 0.02198156680264418 1102 7 0.16039481264364497 1102 8 0.14945308646902333 1102 9 0.5516892512243317 1102 10-11 0.24190253864487232 1102 12-13 0.17438323689490431 1102 14-15 0.2485831259101019 1102 16-17 0.16084387491704888 1102 18-19 0.05438559477713767 1102 20-21 0.2617891578953717 1102 22-23 0.2281127028519947 1102 24-25 0.2520913451642741 1102 26-27 0.14265390204064232 1102 28-29 0.11441722258247466 1102 30-31 0.15019990499731506 1102 32-33 0.08502441823889484 1102 34-35 -0.0014727320410727884 1102 36-37 -0.012610277615820564 1102 38-39 -0.006404410743634514 1102 40-41 0.055425453009320336 1102 42-43 0.0983568071018297 1102 44-45 0.030497013953556973 1102 46-47 0.022425656220526946 1102 48-49 0.13184850636286427 1102 50-51 0.05627283480330192 1102 52-53 -0.049365793933105806 1102 54-55 -0.213509761182209 1102 56-57 -0.05466740076867893 1102 58-59 0.12202063249800688 1102 60-61 -0.06295767265434904 1102 62-63 -0.08268766504170344 1102 64-65 0.062461613735059984 1102 66-67 0.01927861210450743 1102 68-69 0.042484325243513865 1102 70-71 0.1038293982669316 1102 72-73 0.08513429177710918 1102 74-75 0.11721320538919144 1102 76-77 0.08015085280112544 1102 78-79 -0.12700356251045264 1102 80-81 -0.004795701350648329 1102 82-83 -0.04726662001588622 1102 84-85 0.14262714555669476 1102 86-87 0.09156113584455383 1102 88-89 0.11125491029149259 1102 90-91 0.0407228781551332 1102 92-93 0.022304586951701566 1102 94-95 0.10842011073355096 1102 96-97 0.08631622945173234 1102 98-99 0.1085965441672947 1102 100-101 0.07995138332979224 1102 102-103 -0.007191089729726485 1102 104-105 -0.023978397279908137 1102 106-107 -0.009543121973063506 1102 108-109 -0.030695036788458196 1102 110-111 -0.016404215007046474 1102 112-113 -0.050246862335850295 1102 114-115 -0.07299749498517016 1102 116-117 -0.17415378385837244 1102 118-119 0.016540740287130262 1102 120-121 0.0021107478143775893 1102 122-123 0.004115607332416005 1102 124-125 -0.2984623798649757 1103 1 0.34686781913025655 1103 2 0.39165465522117415 1103 3 0.5625270703717717 1103 4 0.48162450277653335 1103 5 0.3366006144789466 1103 6 0.23667054371241392 1103 7 0.42628515113693766 1103 8 0.5026304644689503 1103 9 0.9263471976030146 1103 10-11 0.5943600682287595 1103 12-13 0.44536850305802034 1103 14-15 0.6266124428354374 1103 16-17 0.6003178850491224 1103 18-19 0.6150660278961197 1103 20-21 0.5882880530472718 1103 22-23 0.6056466929295965 1103 24-25 0.4191411805282712 1103 26-27 0.5753651451841719 1103 28-29 0.5164600018358243 1103 30-31 0.7466656029788368 1103 32-33 0.5551699712733296 1103 34-35 0.5175600462851193 1103 36-37 0.5018074555631884 1103 38-39 0.5716975659427774 1103 40-41 0.592620954715386 1103 42-43 0.6207336143465056 1103 44-45 0.5229697651883924 1103 46-47 0.41988191429555144 1103 48-49 0.35315893591806713 1103 50-51 0.4300975265892575 1103 52-53 0.37517313507709105 1103 54-55 0.2548783655950544 1103 56-57 0.25945812676463476 1103 58-59 0.3055754264510071 1103 60-61 0.47384975035630816 1103 62-63 0.46524679072047803 1103 64-65 0.5216142311565122 1103 66-67 0.37808085271891656 1103 68-69 0.4078759967766601 1103 70-71 0.4291693286014535 1103 72-73 0.4114663044687461 1103 74-75 0.45419771347486915 1103 76-77 0.39840021219416855 1103 78-79 0.2996776939117467 1103 80-81 0.28000925529735454 1103 82-83 0.3013567758064539 1103 84-85 0.4890137498732834 1103 86-87 0.39315008348154024 1103 88-89 0.3372163478561738 1103 90-91 0.27795593888340164 1103 92-93 0.3059939312738429 1103 94-95 0.23519892996735337 1103 96-97 0.26838578182444905 1103 98-99 0.2687340957111921 1103 100-101 0.2668636553264747 1103 102-103 0.19480528757843985 1103 104-105 0.1763072808599624 1103 106-107 0.21453149692651508 1103 108-109 0.1854282178919604 1103 110-111 0.21325031292914787 1103 112-113 0.20752894892474316 1103 114-115 0.2252052782145917 1103 116-117 0.13765802612884315 1103 118-119 0.04553353185159992 1103 120-121 0.020644529110317222 1103 122-123 0.07404098390053804 1103 124-125 -0.1756675560736376 1104 1 0.43476926610753 1104 2 0.4837452360954515 1104 3 0.6678795255776429 1104 4 0.46832953794326926 1104 5 0.5172222176509962 1104 6 0.4070190675194638 1104 7 0.4860608127496633 1104 8 0.4533245710220797 1104 9 1.0495705205492953 1104 10-11 0.7208032966712281 1104 12-13 0.5732492427980915 1104 14-15 0.7047577306766897 1104 16-17 0.5559217472431541 1104 18-19 0.5869839828498655 1104 20-21 0.6950969657646979 1104 22-23 0.7262567220330709 1104 24-25 0.6846348095131845 1104 26-27 0.6775728379080519 1104 28-29 0.7583008397557904 1104 30-31 0.7555966628172044 1104 32-33 0.7491257629336303 1104 34-35 0.6881031669647584 1104 36-37 0.5231673981606733 1104 38-39 0.7265255034165605 1104 40-41 0.7009400019219356 1104 42-43 0.709816263864937 1104 44-45 0.60361348099309 1104 46-47 0.5540579318023262 1104 48-49 0.5315632853031858 1104 50-51 0.5761868616251675 1104 52-53 0.39503480536960467 1104 54-55 0.42150998453929134 1104 56-57 0.4095517215370208 1104 58-59 0.404174471599859 1104 60-61 0.45957193581658373 1104 62-63 0.5245391693598762 1104 64-65 0.526117463328319 1104 66-67 0.4872356965835678 1104 68-69 0.46121153836215 1104 70-71 0.5060193800391133 1104 72-73 0.4511651362123885 1104 74-75 0.5085570637713985 1104 76-77 0.5946378696985306 1104 78-79 0.4785140977995752 1104 80-81 0.544245112412181 1104 82-83 0.4802524430711905 1104 84-85 0.48848106886657305 1104 86-87 0.5192319313160105 1104 88-89 0.5627133068105401 1104 90-91 0.43411748062692723 1104 92-93 0.45273837266385897 1104 94-95 0.48710334539083533 1104 96-97 0.4333644921753965 1104 98-99 0.5127096988830466 1104 100-101 0.4813406689809696 1104 102-103 0.35359997271138255 1104 104-105 0.4310782085341067 1104 106-107 0.2952756728318171 1104 108-109 0.20108986675172247 1104 110-111 0.3574989174941372 1104 112-113 0.41715535145567273 1104 114-115 0.4161536852730272 1104 116-117 0.352437204558246 1104 118-119 0.362060749312203 1104 120-121 0.44433669121611885 1104 122-123 0.4196571770608237 1104 124-125 0.12151916649569117 1105 1 0.31107642252911916 1105 2 0.33407091321281257 1105 3 0.4489800279195677 1105 4 0.3059120804428517 1105 5 0.2855332850545196 1105 6 0.2528052795961173 1105 7 0.40060593314515813 1105 8 0.4981432813836335 1105 9 0.9612626615357058 1105 10-11 0.49356122847421346 1105 12-13 0.6518776863666957 1105 14-15 0.298461799836403 1105 16-17 0.4015488167468888 1105 18-19 0.3898094563687806 1105 20-21 0.6723436687231228 1105 22-23 0.7320497481548713 1105 24-25 0.7021318378271388 1105 26-27 0.6807724785532443 1105 28-29 0.6390964788425677 1105 30-31 0.815780813238483 1105 32-33 0.7074208791909413 1105 34-35 0.62653764482247 1105 36-37 0.581123008685978 1105 38-39 0.6701052285487279 1105 40-41 0.5868034688502206 1105 42-43 0.6799233296911282 1105 44-45 0.5272814907631584 1105 46-47 0.614926986455707 1105 48-49 0.5441759371992134 1105 50-51 0.5292742795626921 1105 52-53 0.4765309129740132 1105 54-55 0.5056495850663723 1105 56-57 0.49151650756569865 1105 58-59 0.5819166021769746 1105 60-61 0.5236499242847259 1105 62-63 0.5239847005560279 1105 64-65 0.5359038566336025 1105 66-67 0.4462589669639172 1105 68-69 0.4255992376675337 1105 70-71 0.4627872005312099 1105 72-73 0.4487276537050704 1105 74-75 0.48096311313766194 1105 76-77 0.4635652090305058 1105 78-79 0.36443966107206904 1105 80-81 0.41199898217784536 1105 82-83 0.3589050488806649 1105 84-85 0.5068676403169512 1105 86-87 0.43419659220612417 1105 88-89 0.4330863606482147 1105 90-91 0.422025813748224 1105 92-93 0.41911045925520085 1105 94-95 0.4126337489054599 1105 96-97 0.3613224702124924 1105 98-99 0.3964619374018703 1105 100-101 0.2411015474517555 1105 102-103 0.2524852747573654 1105 104-105 0.24357399350236975 1105 106-107 0.19773577108308515 1105 108-109 0.23885851383932533 1105 110-111 0.36161647736603086 1105 112-113 0.43952703737661736 1105 114-115 0.3301039009360416 1105 116-117 0.3261046184304277 1105 118-119 0.20630240350206108 1105 120-121 0.16588720146078728 1105 122-123 0.13659666722382724 1105 124-125 -0.056876772847960666 1106 1 0.3442381677173394 1106 2 0.4070414369658444 1106 3 0.5119401468801286 1106 4 0.3836141922790901 1106 5 0.3078167210427978 1106 6 0.30026442463095293 1106 7 0.276886895044818 1106 8 0.4359334373433086 1106 9 0.9072434476876197 1106 10-11 0.31319382205736446 1106 12-13 0.5616194006462294 1106 14-15 0.7637797225460972 1106 16-17 0.553250178308204 1106 18-19 0.4122493124893438 1106 20-21 0.6440020723052484 1106 22-23 0.6087174073203414 1106 24-25 0.4704413608627789 1106 26-27 0.5290494967695665 1106 28-29 0.46413741699963396 1106 30-31 0.5346125299402402 1106 32-33 0.5205296795017347 1106 34-35 0.4345706263507694 1106 36-37 0.4980232898359347 1106 38-39 0.6234969142334492 1106 40-41 0.5288989511040327 1106 42-43 0.5485754486083536 1106 44-45 0.3917721763650803 1106 46-47 0.501984637673452 1106 48-49 0.3922656285224164 1106 50-51 0.40617864872427134 1106 52-53 0.26778205541171474 1106 54-55 0.2605830102590474 1106 56-57 0.2936240971440327 1106 58-59 0.4580053037099532 1106 60-61 0.3998765212404436 1106 62-63 0.4877285549957264 1106 64-65 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2216 84-85 -0.35501707595249243 2216 86-87 -0.30350886930819243 2216 88-89 -0.688964166128919 2216 90-91 -0.3734632746408657 2216 92-93 -0.3284887457022414 2216 94-95 -0.3039660668748638 2216 96-97 -0.3092907155744342 2216 98-99 -0.20767286757257253 2216 100-101 -0.3938520366654181 2216 102-103 -0.5745251286878208 2216 104-105 -0.47447857464176124 2216 106-107 -0.22146447078843678 2216 108-109 -0.1587354046585716 2216 110-111 -0.12512474928714568 2216 112-113 -0.3590149494003221 2216 114-115 -0.3109854322942063 2216 116-117 -0.24209646978663102 2216 118-119 -0.13645640133311687 2216 120-121 0.028292243591181432 2216 122-123 0.13726470674862412 2216 124-125 0.2778413014164336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46003.0 3 9348.0 4 5951.0 5 5317.0 6 5417.0 7 6302.0 8 7182.0 9 8075.0 10 8562.0 11 9428.0 12 9735.0 13 10317.0 14 10318.0 15 10086.0 16 9700.0 17 9104.0 18 8706.0 19 8589.0 20 8608.0 21 9327.0 22 10888.0 23 13952.0 24 19546.0 25 26551.0 26 33088.0 27 38723.0 28 45940.0 29 55639.0 30 66972.0 31 80750.0 32 101868.0 33 134100.0 34 182734.0 35 270344.0 36 485016.0 37 771747.0 38 276187.0 39 378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64601535008964 18.52124441618133 12.93742574426075 24.89531448946828 2 15.769036721724614 19.864647296225506 39.39052282889136 24.975793153158516 3 18.28971337685756 24.532759817592495 29.489863137644484 27.687663667905454 4 12.607478911955841 16.41688858854142 37.389560553914166 33.58607194558857 5 13.823479983066603 37.83202893710321 34.72392111646957 13.620569963360612 6 32.78630053157449 36.347243332627784 17.58411396038063 13.2823421754171 7 28.888632038963348 31.93961915222743 21.66472788306458 17.507020925744644 8 26.405073218604926 35.00357034212174 20.23891155266272 18.352444886610613 9 26.610619528641017 15.702877840426622 19.732932267274787 37.953570363657576 10-11 24.804544627720855 26.14754792995838 28.495494482855705 20.55241295946506 12-13 25.53776770052111 23.4797322751032 28.020542948643513 22.96195707573218 14-15 22.805869876462843 25.140992339292133 25.0726324172111 26.980505367033924 16-17 23.222155309331086 28.071917746900148 25.883092089413033 22.82283485435573 18-19 23.171788156214117 27.34034306852505 26.818300770404225 22.669568004856604 20-21 23.65357371456738 26.828134391738907 26.717193298498387 22.80109859519533 22-23 23.66069402366931 26.50154670320319 26.526988233425243 23.31077103970226 24-25 23.448362063921792 26.734357741479663 26.48983304168373 23.32744715291481 26-27 23.352365755822973 26.96079604636217 26.270061830995278 23.416776366819583 28-29 23.391410906334222 26.96236966142375 26.421070323173364 23.22514910906866 30-31 23.475611431870362 26.66798110681416 26.36443054037852 23.491976920936956 32-33 23.224999321911547 26.741858550925862 26.42733187528065 23.60581025188194 34-35 23.77206956177669 26.673266070002132 26.15024799172616 23.404416376495014 36-37 23.82480407122697 26.417256091302306 26.190875903415613 23.567063934055117 38-39 23.370641869079815 26.701818957312195 26.38073993893613 23.54679923467185 40-41 23.642249551622413 26.44613184472742 26.42351131283646 23.488107290813705 42-43 23.49235529846202 26.442202262608284 26.309243223742584 23.756199215187113 44-45 23.446706422501386 26.61180340202587 26.319511329065598 23.621978846407146 46-47 23.721178337625794 26.83456670713305 26.005974937314924 23.438280017926232 48-49 23.587065246412813 26.55882623046847 26.222454983552858 23.63165353956586 50-51 23.48834150136189 26.649974861240427 26.27590728229799 23.585776355099693 52-53 23.723770148851525 26.547091402920298 26.12106685199237 23.608071596235806 54-55 23.512689113397567 26.476185917295826 26.200818549534482 23.81030641977213 56-57 23.333527173494865 26.724890814210234 26.34151279717381 23.600069215121092 58-59 23.490942847027167 26.362251724549367 26.37480389450575 23.77200153391772 60-61 23.31806477188996 26.521395488363485 26.55201416650178 23.60852557324477 62-63 23.371922629923063 26.466282458048656 26.59108377148541 23.570711140542873 64-65 23.353681541093074 26.450164383639358 26.29001798433604 23.906136090931525 66-67 23.23679386118883 26.655567730107904 26.26724678999606 23.840391618707205 68-69 23.301700896528175 26.31377386838273 26.647343481669626 23.73718175341947 70-71 23.35566974121725 26.121524288760504 26.487470739530227 24.035335230492016 72-73 23.29219306494645 26.404449758661723 26.217010128690106 24.08634704770172 74-75 23.38724704952054 26.570954676132075 26.17254575869409 23.8692525156533 76-77 23.456134606216928 26.403074407810045 26.33145121529843 23.809339770674594 78-79 23.400591083398783 26.429494600646397 26.39132415800755 23.77859015794727 80-81 23.491296430223485 26.450221155191244 26.288523528689005 23.769958885896262 82-83 23.443867785610575 26.541779278260968 26.243355228874808 23.77099770725365 84-85 23.488446187732183 26.422877454290855 26.288360303841745 23.800316054135216 86-87 23.487057682934378 26.17504260204855 26.52146184024892 23.81643787476815 88-89 23.463674769278832 26.28380011251707 26.415146861729355 23.83737825647474 90-91 23.34440849253136 26.470670626302024 26.374877000132972 23.810043881033643 92-93 23.466030968986868 26.10294593467114 26.404253444381858 24.02676965196013 94-95 23.259406737517295 26.14218053170771 26.60520644871337 23.993206282061625 96-97 23.36532073953687 25.98126496667198 26.714256258677278 23.939158035113874 98-99 23.35810317883131 26.053049368018797 26.346032643719326 24.242814809430566 100-101 23.199502019720644 25.96082110721539 26.665578088300375 24.17409878476359 102-103 23.18429251664859 25.74041264358585 26.713008444458392 24.362286395307173 104-105 23.026274148609968 26.06779561852266 26.533317613226874 24.3726126196405 106-107 23.16426258129345 25.97289757468786 26.466029958111402 24.39680988590729 108-109 23.192309567391007 25.850242209625453 26.36912480997735 24.58832341300619 110-111 23.14450900254921 26.019307626588734 26.507378954144517 24.328804416717535 112-113 23.245061866616457 26.015448082871533 26.64974245808085 24.08974759243116 114-115 23.06596051861493 26.24898119332071 26.413976015291716 24.271082272772645 116-117 22.920754434646508 26.121398088010523 26.67814122751916 24.279706249823814 118-119 22.960721049807585 26.19727243331213 26.401182561482184 24.440823955398102 120-121 22.915563209541034 26.269298567437065 26.528205738373074 24.286932484648833 122-123 22.908969231063864 26.372753188148085 26.507255124905043 24.21102245588301 124-125 23.017526363647832 26.402351657111844 26.42612838616251 24.153993593077807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 375.0 1 313.5 2 210.5 3 154.0 4 133.0 5 126.0 6 114.5 7 103.0 8 103.0 9 105.5 10 106.0 11 106.0 12 106.5 13 114.0 14 128.5 15 157.5 16 194.5 17 213.5 18 261.5 19 341.0 20 436.5 21 597.0 22 905.0 23 1400.0 24 2046.0 25 3079.5 26 4764.5 27 7545.5 28 11244.5 29 15665.0 30 20918.5 31 27658.5 32 35439.0 33 41048.0 34 45196.0 35 51742.5 36 61600.0 37 70250.5 38 76277.5 39 81680.0 40 86486.0 41 91261.5 42 95198.5 43 98921.0 44 104167.0 45 109989.5 46 111523.0 47 109933.0 48 108764.5 49 107028.5 50 104861.0 51 103508.0 52 104400.5 53 106491.0 54 110238.5 55 106188.5 56 95346.0 57 87414.0 58 81233.5 59 71320.0 60 58218.5 61 48245.5 62 39639.5 63 30795.0 64 22857.5 65 16954.0 66 12313.0 67 9611.0 68 7294.5 69 5167.5 70 3775.0 71 2498.5 72 1835.0 73 1325.0 74 951.5 75 718.0 76 494.0 77 292.0 78 145.5 79 88.0 80 55.5 81 36.5 82 25.5 83 16.0 84 13.0 85 17.0 86 12.5 87 5.0 88 6.0 89 5.0 90 5.5 91 7.5 92 7.0 93 4.5 94 2.5 95 2.5 96 1.0 97 0.5 98 2.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0898422902622161 2 5.318209762956755E-4 3 0.0 4 0.0021627386369357468 5 0.001276370343109621 6 3.1909258577740525E-4 7 3.545473175304503E-5 8 0.001418189270121801 9 5.318209762956755E-4 10-11 0.010246417476630014 12-13 0.002322284929824449 14-15 0.0046800245914019436 16-17 0.00288956063787317 18-19 0.014890987336278912 20-21 0.018897372024373 22-23 0.01084914791643178 24-25 0.011806425673763995 26-27 0.011008694209320482 28-29 0.008757318743002122 30-31 0.01912782778076779 32-33 0.0024641038568366295 34-35 0.0014536440018748462 36-37 7.622767326904682E-4 38-39 3.013652199008827E-4 40-41 0.001825918685281819 42-43 0.0028541059061201247 44-45 0.0011168240502209183 46-47 0.0017727365876522515 48-49 0.008899137670014302 50-51 0.0053891192264628445 52-53 0.008438226157224718 54-55 0.009413231280433455 56-57 0.010813693184678733 58-59 0.00951959547569259 60-61 0.01148733308798659 62-63 0.01473144104339021 64-65 0.00943095864630998 66-67 0.014465530555242372 68-69 0.014075528505958876 70-71 0.004963662445426304 72-73 0.013827345383687562 74-75 0.01437689372585976 76-77 0.006470488544930718 78-79 0.008970047133520393 80-81 0.01487325997040239 82-83 0.018347823682200802 84-85 0.01458962211637803 86-87 0.01788691216941122 88-89 0.017071453339091183 90-91 0.013224614943885797 92-93 0.010264144842506536 94-95 0.008704136645372553 96-97 0.009696869134457815 98-99 0.007108673716485528 100-101 0.01084914791643178 102-103 0.012923249723984913 104-105 0.011717788844381382 106-107 0.005672757080487204 108-109 0.003917747858711476 110-111 0.007569585229275115 112-113 0.009838688061469996 114-115 0.01451871265287194 116-117 0.012285064552430103 118-119 0.22223025862808626 120-121 0.02699877822994379 122-123 0.029640155745545644 124-125 0.24188990738514973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2820498.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.00212780137973 #Duplication Level Percentage of deduplicated Percentage of total 1 85.23635642631129 59.667263158785964 2 8.757360566929071 12.260677472178642 3 2.6771802451857036 5.622249410124562 4 1.1912037379771458 3.335471852134296 5 0.6195658289305064 2.168546316908054 6 0.34267891952139984 1.439295211150545 7 0.2290769373390562 1.1225111130770087 8 0.16027237349739973 0.8975525738076353 9 0.11707716833074698 0.7376085810101336 >10 0.598809597214723 7.623424405233995 >50 0.04697099581388297 2.2848268084688668 >100 0.023200391684810397 2.7314668613171653 >500 2.468112643004051E-4 0.10910623580317119 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.545473175304503E-5 0.0 0.0 0.0 0.0 8 3.545473175304503E-5 0.0 0.0 0.0 0.0 9 3.545473175304503E-5 0.0 0.0 0.0 0.0 10-11 3.545473175304503E-5 0.0 0.0 0.0 0.0 12-13 5.3182097629567543E-5 0.0 0.0 0.0 0.0 14-15 1.4181892701218012E-4 0.0 0.0 0.0 5.3182097629567543E-5 16-17 1.5954629288870265E-4 0.0 0.0 0.0 7.090946350609006E-5 18-19 1.7727365876522516E-4 0.0 0.0 0.0 7.090946350609006E-5 20-21 1.7727365876522516E-4 0.0 0.0 0.0 7.090946350609006E-5 22-23 1.7727365876522516E-4 0.0 0.0 7.090946350609006E-5 7.090946350609006E-5 24-25 2.1272839051827017E-4 0.0 0.0 1.5954629288870265E-4 7.090946350609006E-5 26-27 2.1272839051827017E-4 0.0 0.0 4.6091151278958536E-4 7.090946350609006E-5 28-29 3.013652199008827E-4 0.0 0.0 0.002552740686219242 7.090946350609006E-5 30-31 3.1909258577740525E-4 0.0 0.0 0.00888141030413778 7.090946350609006E-5 32-33 3.1909258577740525E-4 0.0 0.0 0.021893296857505306 7.090946350609006E-5 34-35 3.9000204928349533E-4 0.0 0.0 0.04082612361363135 7.090946350609006E-5 36-37 4.6091151278958536E-4 0.0 0.0 0.0746322103401598 7.090946350609006E-5 38-39 5.672757080487205E-4 0.0 0.0 0.12623657240671682 7.090946350609006E-5 40-41 5.672757080487205E-4 0.0 0.0 0.18285777901632974 7.090946350609006E-5 42-43 7.090946350609006E-4 0.0 0.0 0.2414289958723601 7.090946350609006E-5 44-45 8.509135620730807E-4 0.0 0.0 0.3152457473821999 7.090946350609006E-5 46-47 0.001170006147850486 0.0 0.0 0.40452076193636727 7.090946350609006E-5 48-49 0.001382734538368756 0.0 0.0 0.4988835304970966 1.4181892701218012E-4 50-51 0.001382734538368756 0.0 0.0 0.5990963297970784 1.4181892701218012E-4 52-53 0.001382734538368756 0.0 0.0 0.7012591393434777 1.5954629288870265E-4 54-55 0.0014181892701218013 0.0 0.0 0.8101938026547084 1.7727365876522516E-4 56-57 0.0014890987336278912 0.0 0.0 0.9407026702376673 1.7727365876522516E-4 58-59 0.0014890987336278912 0.0 0.0 1.0852693389607082 1.7727365876522516E-4 60-61 0.0014890987336278912 0.0 0.0 1.2407383376978107 1.7727365876522516E-4 62-63 0.0014890987336278912 0.0 0.0 1.3961186996055306 1.7727365876522516E-4 64-65 0.0014890987336278912 0.0 0.0 1.5605932002079066 1.7727365876522516E-4 66-67 0.0016309176606400715 0.0 0.0 1.7481487311815147 1.7727365876522516E-4 68-69 0.0016309176606400715 0.0 0.0 1.9617812173594875 1.7727365876522516E-4 70-71 0.001755009221775729 0.0 0.0 2.1936906177561553 1.7727365876522516E-4 72-73 0.0018081913194052965 0.0 0.0 2.434375064261701 2.481831222713152E-4 74-75 0.0018081913194052965 0.0 0.0 2.6815477266780547 2.481831222713152E-4 76-77 0.0018259186852818189 0.0 0.0 2.9496564081945813 2.481831222713152E-4 78-79 0.0018436460511583415 0.0 0.0 3.256073927370273 2.481831222713152E-4 80-81 0.0020031923440470443 0.0 0.0 3.582027003741892 2.6591048814783775E-4 82-83 0.002091829173429657 0.0 0.0 3.9178010408091053 3.1909258577740525E-4 84-85 0.002091829173429657 0.0 0.0 4.2656651414041065 3.1909258577740525E-4 86-87 0.002091829173429657 0.0 0.0 4.628898868214053 3.1909258577740525E-4 88-89 0.002091829173429657 0.0 0.0 5.02159547711078 3.1909258577740525E-4 90-91 0.0021272839051827016 0.0 0.0 5.444020878582435 3.1909258577740525E-4 92-93 0.0021804660028122693 0.0 0.0 5.883588642856687 3.1909258577740525E-4 94-95 0.002198193368688792 0.0 0.0 6.334714649682432 3.1909258577740525E-4 96-97 0.002198193368688792 0.0 0.0 6.803585749750576 3.1909258577740525E-4 98-99 0.002198193368688792 0.0 0.0 7.297275162045851 3.1909258577740525E-4 100-101 0.002322284929824449 0.0 0.0 7.833013886200239 3.1909258577740525E-4 102-103 0.0024641038568366295 0.0 0.0 8.397648216733357 3.545473175304503E-4 104-105 0.00288956063787317 0.0 0.0 8.976517622065323 3.545473175304503E-4 106-107 0.00296047010137926 0.0 0.0 9.551540189002084 3.545473175304503E-4 108-109 0.002995924833132305 0.0 0.0 10.157674283052142 3.545473175304503E-4 110-111 0.00310228902839144 0.0 0.0 10.801266301199291 3.545473175304503E-4 112-113 0.003155471126021008 0.0 0.0 11.45817157111971 3.545473175304503E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2110 0.0 43.449574 1 GTATCAA 3195 0.0 42.29629 1 ATCAACG 3225 0.0 40.57108 3 CAACGCA 3290 0.0 39.593666 5 TCAACGC 3500 0.0 37.216064 4 AACGCAG 3595 0.0 36.399994 6 GTACATG 9120 0.0 33.290676 1 TATCAAC 4095 0.0 32.82299 2 TACATGG 9295 0.0 31.928223 2 ACATGGG 9335 0.0 30.389786 3 ACGCAGA 4415 0.0 29.504158 7 CATGGGG 6655 0.0 27.97375 4 CGCAGAG 4655 0.0 27.727446 8 TAGTCCG 155 2.1119091E-4 23.024862 5 GTGTAAG 1560 0.0 22.515142 1 GAGTACA 7540 0.0 20.449171 1 AGTACAT 7120 0.0 19.880192 2 GTATAGA 845 0.0 19.726435 1 GCAGAGT 6810 0.0 18.953531 9 CAGAGTA 5885 0.0 18.445646 10-11 >>END_MODULE