FastQCFastQC Report
Thu 26 May 2016
SRR1364412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820498
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.055.878071
GTATCAA46350.047.1886561
ATCAACG57900.037.8541073
CAACGCA58950.036.9463275
TCAACGC59750.036.4814034
AACGCAG61100.035.5431826
TATCAAC62800.034.5230062
GTACATG87850.032.562731
TACATGG91600.030.4960962
ACGCAGA71950.030.1833027
ACATGGG91850.029.7630393
CGCAGAG73750.029.5257288
CATGGGG65300.024.169134
GCAGAGT94650.022.5660429
CAGAGTA84500.021.93223610-11
AGAGTAC86200.021.29263910-11
CATGGGA40200.019.8521294
GAGTACT50600.019.45938312-13
GTACTTT50500.018.61432514-15
AGTACAT69000.018.4727522
Produced by FastQC (version 0.11.5)