FastQCFastQC Report
Thu 26 May 2016
SRR1364411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708911
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33850.056.979081
GGTATCA18300.054.6496351
ATCAACG38500.049.0076753
TCAACGC39100.048.103414
CAACGCA40600.046.179595
AACGCAG42450.044.2981876
TATCAAC43550.044.0081632
GTACATG102850.039.2423021
TACATGG106550.037.1478542
ACATGGG103900.036.4913063
ACGCAGA52350.035.799877
CGCAGAG52850.035.2349978
CATGGGA74600.034.0686234
ATGGGAT30400.030.347555
GCAGAGT63250.028.6889219
CATGGGG33250.028.1043514
ATGGGAG28950.027.3444165
TAAGGTG6950.023.9794245
GTATAGG8100.023.5176581
GAGTACA74400.023.0435121
Produced by FastQC (version 0.11.5)