FastQCFastQC Report
Thu 26 May 2016
SRR1364411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708911
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.1838597797076618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26530.15524506542470615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27850.076.3287051
GTATCAA52650.053.8336071
ATCAACG66750.042.183283
TCAACGC68150.041.3167154
CAACGCA69750.040.28365
TATCAAC70550.040.079942
AACGCAG71600.039.2278256
GTACATG94700.035.4628831
CGCAGAG85650.032.9270368
TACATGG101200.032.8234442
ACGCAGA86350.032.5912137
ACATGGG97900.031.923253
GTGGTAT11800.031.7909451
CATGGGA70300.029.2142144
GCAGAGT98200.028.5371849
GAGTACT48800.025.54264612-13
CGTATAG2809.276846E-1125.5192621
CATGGGG32050.025.4462054
CAGAGTA95300.025.40991410-11
TGGTATC14000.025.087332
Produced by FastQC (version 0.11.5)