FastQCFastQC Report
Thu 26 May 2016
SRR1364410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083432
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.050.9807051
GTATCAA25200.039.690461
GTACATG69850.036.9913941
ACATGGG70050.035.0050663
ATCAACG26750.034.9315343
TACATGG71750.034.922232
TCAACGC27000.034.6080934
CAACGCA27050.034.3232735
AACGCAG28900.031.9163426
TATCAAC30150.031.3871292
CATGGGG45100.030.088474
ACGCAGA34650.026.6135887
CGCAGAG36100.025.8736118
ATTCCGT3451.8189894E-1224.148396
TAAGGTG10900.024.0246775
CATGGGA32900.023.8792044
TATTCCG3103.3651304E-1023.0383265
GAGTACA50850.021.8941461
GTGTAAG11450.021.8384191
TCCGTTG3609.458745E-1121.483528
Produced by FastQC (version 0.11.5)