FastQCFastQC Report
Thu 26 May 2016
SRR1364410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083432
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32490.15594461446305902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.15546463719478246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.074.029121
GTATCAA48850.051.9351731
ATCAACG60900.041.159173
TCAACGC62500.040.1054954
CAACGCA63000.039.7871975
TATCAAC64500.039.046532
AACGCAG65500.038.3374066
GTACATG68750.033.0041731
ACGCAGA77800.032.1967
CGCAGAG78800.031.7874228
TACATGG71650.030.6628112
ACATGGG69850.030.6005923
GCAGAGT95100.026.0888529
GGTCGAC1150.001005324325.8685237
TAAGGTG10500.025.516735
CAGAGTA86000.025.18270510-11
AGAGTAC83600.024.90928310-11
CATGGGG45300.024.5779274
GAGTACT60850.024.34659612-13
GTGGTAT15200.024.2920761
Produced by FastQC (version 0.11.5)