FastQCFastQC Report
Thu 26 May 2016
SRR1364409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891418
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.047.005841
GTATCAA24400.045.6228561
ATCAACG25900.041.589453
CAACGCA26450.040.7246445
TCAACGC28100.038.333344
AACGCAG28850.037.54116
GTACATG64650.036.4634171
TATCAAC31500.034.3846972
TACATGG69750.033.3608362
ACATGGG68500.032.9270633
ACGCAGA34500.031.0423417
CGCAGAG35150.030.4666888
CATGGGG37350.027.8837914
CATGGGA39350.026.1640994
GCAGAGT44900.023.1883479
ATGGGGA21450.021.3632765
GAGTACA53850.020.4511051
CAGAGTA43250.020.22131210-11
TAGACCG1805.777685E-419.8373245
AGAGTAC42550.019.64512610-11
Produced by FastQC (version 0.11.5)