##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364409_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1891418 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.189892979764387 31.0 31.0 34.0 28.0 34.0 2 31.195800716710956 33.0 31.0 34.0 28.0 34.0 3 31.31246926908806 33.0 31.0 34.0 28.0 34.0 4 34.7424757509974 37.0 35.0 37.0 32.0 37.0 5 34.81863501351896 37.0 35.0 37.0 32.0 37.0 6 34.94837894109076 37.0 35.0 37.0 32.0 37.0 7 34.83831389994174 37.0 35.0 37.0 32.0 37.0 8 34.79265080484589 37.0 35.0 37.0 32.0 37.0 9 36.37678662252342 39.0 37.0 39.0 32.0 39.0 10-11 36.27905439199584 39.0 37.0 39.0 32.0 39.0 12-13 36.29956440088864 39.0 37.0 39.0 32.0 39.0 14-15 37.615849325743966 40.0 38.0 41.0 32.0 41.0 16-17 37.623653787793074 40.0 38.0 41.0 32.0 41.0 18-19 37.629696344224286 40.0 38.0 41.0 32.0 41.0 20-21 37.676245018287865 40.0 38.0 41.0 32.0 41.0 22-23 37.62754161163741 40.0 38.0 41.0 32.0 41.0 24-25 37.64297051207084 40.0 38.0 41.0 32.0 41.0 26-27 37.6089304426626 40.0 38.0 41.0 32.0 41.0 28-29 37.61670238942423 40.0 38.0 41.0 32.0 41.0 30-31 37.52470078004968 40.0 38.0 41.0 31.0 41.0 32-33 37.49101943621135 40.0 38.0 41.0 31.0 41.0 34-35 37.477974197136746 40.0 38.0 41.0 31.0 41.0 36-37 37.41315298892154 40.0 38.0 41.0 31.0 41.0 38-39 37.318606199158516 40.0 37.0 41.0 31.0 41.0 40-41 37.262832700122345 40.0 37.0 41.0 30.0 41.0 42-43 37.12858923833865 40.0 37.0 41.0 30.0 41.0 44-45 36.99526043423505 40.0 37.0 41.0 30.0 41.0 46-47 36.881819090227545 40.0 37.0 41.0 30.0 41.0 48-49 36.74040138139745 40.0 36.0 41.0 30.0 41.0 50-51 36.62076098461577 40.0 36.0 41.0 30.0 41.0 52-53 36.47242122048114 40.0 36.0 41.0 30.0 41.0 54-55 36.299745746313086 39.0 35.0 41.0 29.5 41.0 56-57 36.104498053841084 39.0 35.0 41.0 29.0 41.0 58-59 35.88675083984609 39.0 35.0 41.0 29.0 41.0 60-61 35.644583058847914 38.5 35.0 40.5 28.5 41.0 62-63 35.37827677435659 38.0 34.5 40.0 28.0 41.0 64-65 35.041577007303516 37.5 34.0 40.0 28.0 41.0 66-67 34.73082153178198 37.0 34.0 40.0 28.0 41.0 68-69 34.404301164523126 36.5 34.0 39.5 28.0 41.0 70-71 34.04934683924971 36.0 34.0 39.0 27.0 41.0 72-73 33.686523550056094 35.5 33.5 39.0 26.0 40.0 74-75 33.32581586936362 35.0 33.0 37.5 26.0 40.0 76-77 33.08937527294337 35.0 33.0 37.0 26.0 39.0 78-79 32.93003476756592 35.0 33.0 37.0 26.0 39.0 80-81 32.661077033210006 35.0 33.0 36.0 26.0 39.0 82-83 32.37415447034976 35.0 33.0 36.0 26.0 37.0 84-85 32.12956363955509 35.0 33.0 35.5 26.0 37.0 86-87 31.92567613293307 35.0 33.0 35.0 26.0 36.5 88-89 31.672474302348817 35.0 33.0 35.0 25.0 36.0 90-91 31.558286428489104 35.0 33.0 35.0 25.0 36.0 92-93 31.423687942062514 35.0 32.5 35.0 25.0 36.0 94-95 31.33615335161239 35.0 32.0 35.0 25.0 35.5 96-97 31.24465268914645 35.0 32.0 35.0 24.0 35.0 98-99 31.13431140022988 35.0 32.0 35.0 24.0 35.0 100-101 31.063188306339477 35.0 32.0 35.0 23.5 35.0 102-103 30.031422456590768 33.5 30.0 34.5 20.0 35.0 104-105 30.773862255725597 34.5 31.5 35.0 23.0 35.0 106-107 31.136933507030175 35.0 32.5 35.0 24.0 35.0 108-109 31.19218464665135 35.0 33.0 35.0 24.0 35.0 110-111 31.14149807181702 35.0 33.0 35.0 24.0 35.0 112-113 31.073155695885312 35.0 33.0 35.0 23.5 35.0 114-115 30.99708023292577 35.0 33.0 35.0 23.0 35.0 116-117 30.88734668909781 35.0 33.0 35.0 20.0 35.0 118-119 30.733995869765437 35.0 32.5 35.0 19.0 35.0 120-121 30.58529447218965 35.0 32.0 35.0 17.5 35.0 122-123 30.423904446293733 35.0 32.0 35.0 4.5 35.0 124-125 29.616507826403257 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1606946804731244 1101 2 0.1860593961366348 1101 3 0.2073758188521957 1101 4 0.2945715911097153 1101 5 0.26875302152837577 1101 6 0.25502098171966026 1101 7 0.25747927804766135 1101 8 0.1558361135683839 1101 9 0.341058487972262 1101 10-11 0.3819628555330894 1101 12-13 0.4184506666228245 1101 14-15 0.5074419837113453 1101 16-17 0.48015169969085747 1101 18-19 0.2701492414389719 1101 20-21 0.4687196297248164 1101 22-23 0.4782419760825789 1101 24-25 0.498307722605162 1101 26-27 0.6325516027372302 1101 28-29 0.5269966874515859 1101 30-31 0.5557084995749477 1101 32-33 0.43749739171481394 1101 34-35 0.4522266846782941 1101 36-37 0.433641859540586 1101 38-39 0.4916328279794939 1101 40-41 0.3815445566985929 1101 42-43 0.4332812042648584 1101 44-45 0.48076734786872066 1101 46-47 0.401061976911123 1101 48-49 0.20799665568198122 1101 50-51 0.23918982844226377 1101 52-53 0.20340241789866553 1101 54-55 0.33831080866512764 1101 56-57 0.2982533421620843 1101 58-59 0.27070934052957085 1101 60-61 0.2285572297182341 1101 62-63 0.162072932393734 1101 64-65 0.23466777737077393 1101 66-67 0.2558064506643589 1101 68-69 0.20375736671145717 1101 70-71 0.20347928216501288 1101 72-73 0.20918671286081292 1101 74-75 0.17132872151044864 1101 76-77 0.24326227679208046 1101 78-79 0.18523350488086976 1101 80-81 0.32169517763124134 1101 82-83 0.2695168880467591 1101 84-85 0.21766729686298447 1101 86-87 0.18659992362259104 1101 88-89 0.21917756201278493 1101 90-91 0.14601385067357242 1101 92-93 0.11046108831354573 1101 94-95 0.15136176945302182 1101 96-97 0.16034491936096984 1101 98-99 0.10298741721178217 1101 100-101 0.14113490790347782 1101 102-103 -0.16867060097686348 1101 104-105 0.0445976453470891 1101 106-107 0.05107220761871645 1101 108-109 0.09796564550076425 1101 110-111 0.11449956995674526 1101 112-113 0.1984978882216133 1101 114-115 0.1517542274804491 1101 116-117 0.15237870059407754 1101 118-119 0.17931350281897807 1101 120-121 0.08922357074693821 1101 122-123 0.08455611597256762 1101 124-125 -0.024820014288504666 1102 1 0.026197589334827853 1102 2 0.027729033204256126 1102 3 0.07363771177816503 1102 4 0.16908054253952542 1102 5 0.1267779792900683 1102 6 0.09042533720658952 1102 7 0.025259458786571543 1102 8 -0.047239676678152875 1102 9 0.056335033633054366 1102 10-11 0.13077389594066346 1102 12-13 0.06567053117253607 1102 14-15 0.13636630961681107 1102 16-17 0.17842343250288906 1102 18-19 0.13815114995487932 1102 20-21 0.16173508486750166 1102 22-23 0.13610173692666905 1102 24-25 0.17389398384806043 1102 26-27 0.28957791969046554 1102 28-29 0.2347851586727927 1102 30-31 0.30699456330617636 1102 32-33 0.27424095846571817 1102 34-35 0.14169071245674303 1102 36-37 0.05318557931375523 1102 38-39 0.24105539801835363 1102 40-41 0.17625388631505956 1102 42-43 0.0856618407671661 1102 44-45 0.07901728886031378 1102 46-47 0.09208060332312584 1102 48-49 0.1195710161400001 1102 50-51 0.037923718346149826 1102 52-53 0.038350290691973044 1102 54-55 0.08548839495193761 1102 56-57 -0.025281264474244836 1102 58-59 0.005421898958552163 1102 60-61 -0.05347368269836039 1102 62-63 0.035104542255140814 1102 64-65 -0.011148352945369311 1102 66-67 0.015457989332055888 1102 68-69 -0.056352785879241196 1102 70-71 -0.13784549534796042 1102 72-73 -0.12794020877548462 1102 74-75 -0.058793568431582344 1102 76-77 -0.02375878668073028 1102 78-79 0.10081803982797055 1102 80-81 -0.022653281569837702 1102 82-83 0.006106526755424113 1102 84-85 -0.009578499420257458 1102 86-87 -0.046818046157639515 1102 88-89 0.01401809543818544 1102 90-91 -0.052667344583802844 1102 92-93 -0.007737958920355226 1102 94-95 -0.09555554044531434 1102 96-97 -0.11076530728994527 1102 98-99 -0.10904166767561563 1102 100-101 -0.23575222911485838 1102 102-103 -0.36189358458078047 1102 104-105 -0.2572060140803245 1102 106-107 -0.2586480365562309 1102 108-109 -0.21551288849047978 1102 110-111 -0.25781874407031324 1102 112-113 -0.24849817060244916 1102 114-115 -0.27998848944274 1102 116-117 -0.3404678279795803 1102 118-119 -0.37637472176570697 1102 120-121 -0.4613021502612824 1102 122-123 -0.4761955100222508 1102 124-125 -0.5295340272815423 1103 1 0.19089298480816552 1103 2 0.18629353668983129 1103 3 0.12431949003833864 1103 4 0.2849983149165638 1103 5 0.25947030210768673 1103 6 0.16805551215396264 1103 7 0.03345311999219547 1103 8 0.11148448954937606 1103 9 0.1799748198055795 1103 10-11 0.3027973917944138 1103 12-13 0.21155941219626584 1103 14-15 0.19842359944008336 1103 16-17 0.2710461748743356 1103 18-19 0.24654170232504669 1103 20-21 0.2780136059028706 1103 22-23 0.3398306091414227 1103 24-25 0.3729446590720258 1103 26-27 0.43765501922931094 1103 28-29 0.2822439255730558 1103 30-31 0.44897616407590846 1103 32-33 0.4955071705261531 1103 34-35 0.4038575901718602 1103 36-37 0.2900862869159653 1103 38-39 0.27654811094789267 1103 40-41 0.34043699771979874 1103 42-43 0.35317388101580605 1103 44-45 0.2603384521698615 1103 46-47 0.23157864883094703 1103 48-49 0.3051206203913779 1103 50-51 0.22649485478307696 1103 52-53 0.19006620636697846 1103 54-55 0.20682539344969086 1103 56-57 0.20435662357306938 1103 58-59 0.18055370592656317 1103 60-61 0.06273234778175407 1103 62-63 0.02440504116843556 1103 64-65 0.1046134270975756 1103 66-67 0.07996239251063031 1103 68-69 0.03597619277734054 1103 70-71 0.0990392197628438 1103 72-73 0.09565277969682029 1103 74-75 0.05547907470776181 1103 76-77 0.06491590769226718 1103 78-79 0.17810188557646 1103 80-81 0.11853931259579298 1103 82-83 0.10382563044182547 1103 84-85 -0.07545720817076784 1103 86-87 0.011243349278995396 1103 88-89 0.05398411794827496 1103 90-91 -0.010012355057572364 1103 92-93 -0.09040131173307131 1103 94-95 -0.10947438248406982 1103 96-97 -0.11160006290632296 1103 98-99 0.007901852612562266 1103 100-101 -0.07415229832826498 1103 102-103 -0.2665817441071958 1103 104-105 -0.12576543142929353 1103 106-107 -0.1164711176538944 1103 108-109 -0.006212730400768862 1103 110-111 -0.011339058466965923 1103 112-113 0.018312297400772337 1103 114-115 -0.03745226382801192 1103 116-117 -0.16027177576540907 1103 118-119 -0.15078031232122413 1103 120-121 -0.3531243504610586 1103 122-123 -0.32363933311908255 1103 124-125 -0.44543921540305575 1104 1 0.26487140644001883 1104 2 0.22083975327576155 1104 3 0.2750929377431959 1104 4 0.3231710961398875 1104 5 0.3478899283431929 1104 6 0.27053399035445835 1104 7 0.24880842253161717 1104 8 0.29263658721772146 1104 9 0.38134480962322215 1104 10-11 0.4033494503789683 1104 12-13 0.2590256509982467 1104 14-15 0.4237489977347977 1104 16-17 0.4404094947340482 1104 18-19 0.4299749307492462 1104 20-21 0.380238939400769 1104 22-23 0.41040205534464036 1104 24-25 0.40128002373410254 1104 26-27 0.4327263471526237 1104 28-29 0.38172876517522525 1104 30-31 0.3635207350284162 1104 32-33 0.5039418131665698 1104 34-35 0.36382215762740344 1104 36-37 0.3565066228308851 1104 38-39 0.3297306116935701 1104 40-41 0.38838239868866964 1104 42-43 0.30653637291620583 1104 44-45 0.25006918540240264 1104 46-47 0.18338486006855703 1104 48-49 0.18141462822870835 1104 50-51 0.16934444903159118 1104 52-53 0.15949226600061905 1104 54-55 0.08349828266770487 1104 56-57 0.055653459842837094 1104 58-59 0.1576480315768407 1104 60-61 0.05908636981266824 1104 62-63 0.10298133684762689 1104 64-65 0.2088847457641947 1104 66-67 0.162928801018559 1104 68-69 0.11978611697534802 1104 70-71 -0.07785582996613982 1104 72-73 -0.04794467779956335 1104 74-75 -0.08802028760928238 1104 76-77 -0.05585545725779184 1104 78-79 0.12570491765602299 1104 80-81 0.16944492523425225 1104 82-83 0.057451423688554826 1104 84-85 0.06834312507000107 1104 86-87 0.08038155163019312 1104 88-89 0.13203175389302046 1104 90-91 0.0031849821018603564 1104 92-93 -0.0586813419715817 1104 94-95 -0.14138398578356615 1104 96-97 0.004154948561680527 1104 98-99 0.013853003013171161 1104 100-101 0.019547754126300276 1104 102-103 -0.09001980657955144 1104 104-105 -0.07055443036991704 1104 106-107 -0.08248120230116385 1104 108-109 -0.027342534878947333 1104 110-111 -0.06002602299328075 1104 112-113 -0.05809330495248588 1104 114-115 -0.1271757974143597 1104 116-117 -0.2049237242982258 1104 118-119 -0.2417279143247839 1104 120-121 -0.24521785329463341 1104 122-123 -0.35327989078310296 1104 124-125 -0.5308049060034001 1105 1 -0.08081361646019403 1105 2 -0.07010564684685505 1105 3 -0.08184384324421856 1105 4 -0.010193792604752616 1105 5 -0.07429893530136411 1105 6 0.14196372096140664 1105 7 0.07959929129716414 1105 8 0.10804193901547876 1105 9 0.06572758577814852 1105 10-11 0.023232965832107766 1105 12-13 0.10490000636627173 1105 14-15 0.15398854838573328 1105 16-17 0.08820827939992171 1105 18-19 0.020442040994844035 1105 20-21 0.07796740057859353 1105 22-23 0.07429635373261334 1105 24-25 0.07309698431689071 1105 26-27 0.1146838645531787 1105 28-29 0.1254424764522284 1105 30-31 0.1439762148509942 1105 32-33 0.128378323679037 1105 34-35 -0.008247697037390367 1105 36-37 -0.027085464875128196 1105 38-39 0.017543911848640903 1105 40-41 0.003760252199434433 1105 42-43 0.058010461415612724 1105 44-45 -8.859165410513015E-5 1105 46-47 -0.013825190780735852 1105 48-49 -0.0597205551533051 1105 50-51 0.02633757896775535 1105 52-53 -0.02789052818597071 1105 54-55 -0.02526420425933651 1105 56-57 -0.10084109459476309 1105 58-59 -0.09722051759550965 1105 60-61 -0.14034157565958338 1105 62-63 -0.19286191390068552 1105 64-65 -0.08091338976178974 1105 66-67 -0.14206777296536188 1105 68-69 -0.1776033489267732 1105 70-71 -0.20339227195827192 1105 72-73 -0.17926026797590566 1105 74-75 -0.25925572704092303 1105 76-77 -0.20720197173722 1105 78-79 -0.18089647438841183 1105 80-81 -0.1763032084885694 1105 82-83 -0.1721773043578949 1105 84-85 -0.17946602780244802 1105 86-87 -0.21144740000888262 1105 88-89 -0.13771517170833292 1105 90-91 -0.1593637033898645 1105 92-93 -0.1688660763651768 1105 94-95 -0.21978565912194625 1105 96-97 -0.19076057309434802 1105 98-99 -0.22689900751731784 1105 100-101 -0.22087743250141756 1105 102-103 -0.3572513151085275 1105 104-105 -0.2260189260242882 1105 106-107 -0.1595643102387001 1105 108-109 -0.12972857476498945 1105 110-111 -0.2031996859454246 1105 112-113 -0.26487366125815726 1105 114-115 -0.2886628687115582 1105 116-117 -0.36255652750064726 1105 118-119 -0.2782803504025395 1105 120-121 -0.32888026448868857 1105 122-123 -0.40338312205722104 1105 124-125 -0.4940022805931825 1106 1 0.2359486812703615 1106 2 0.23611417090900133 1106 3 0.26301615356890196 1106 4 0.24681524659042253 1106 5 0.2303841748763773 1106 6 0.24305712663592516 1106 7 0.25229722687976874 1106 8 0.2741299353806568 1106 9 0.18661331639997059 1106 10-11 0.3665133974520671 1106 12-13 0.2767452295736632 1106 14-15 0.27863378599821687 1106 16-17 0.2992758669071591 1106 18-19 0.2913091979279585 1106 20-21 0.21739265714462874 1106 22-23 0.3467933950155313 1106 24-25 0.34922557640168606 1106 26-27 0.48358519510021125 1106 28-29 0.4074920176352492 1106 30-31 0.4910974287661105 1106 32-33 0.39495558751075777 1106 34-35 0.3725114414394497 1106 36-37 0.41702395018440797 1106 38-39 0.3188578584935584 1106 40-41 0.25088472205998613 1106 42-43 0.21573265132504105 1106 44-45 0.2289577345294589 1106 46-47 0.2514924291626244 1106 48-49 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82-83 -0.5765924294147062 2216 84-85 -0.6256291567735701 2216 86-87 -0.6282085381480123 2216 88-89 -0.5218972608033177 2216 90-91 -0.622877143341455 2216 92-93 -0.5942959611258054 2216 94-95 -0.5557257404718534 2216 96-97 -0.5196088060189048 2216 98-99 -0.6471449878115507 2216 100-101 -0.5397610613706334 2216 102-103 -0.5396803175024374 2216 104-105 -0.5862854906554347 2216 106-107 -0.39119364405815915 2216 108-109 -0.4646995718646991 2216 110-111 -0.478856207293763 2216 112-113 -0.5170787868340057 2216 114-115 -0.5242058850385085 2216 116-117 -0.5381907048424317 2216 118-119 -0.4814680742382116 2216 120-121 -0.3152944122325785 2216 122-123 -0.4047569936215929 2216 124-125 -0.33879403824996146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26484.0 3 4941.0 4 3135.0 5 2650.0 6 2939.0 7 3403.0 8 3952.0 9 4359.0 10 4726.0 11 5502.0 12 6121.0 13 6356.0 14 6344.0 15 6027.0 16 5339.0 17 5060.0 18 4757.0 19 4465.0 20 4646.0 21 4723.0 22 5572.0 23 7233.0 24 10612.0 25 15438.0 26 18807.0 27 19200.0 28 22637.0 29 28280.0 30 33664.0 31 41736.0 32 54649.0 33 73267.0 34 104304.0 35 160136.0 36 315344.0 37 603361.0 38 260681.0 39 568.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63494320285541 18.711480237894243 13.047898325811863 24.605678233438486 2 15.44692663582287 20.302392597488435 39.67381361533378 24.576867151354925 3 18.449197295485355 24.936870689600475 29.596279071734777 27.017652943179392 4 12.350070771090287 16.559166164809664 37.90031137164789 33.19045169245216 5 13.122500255163693 38.51866502586276 34.985242812713025 13.373591906260526 6 32.17233337035333 36.90729664122864 17.756535351451976 13.163834636966051 7 28.17567299634389 32.43230382450677 21.75561964834735 17.636403530801992 8 25.8850903846164 35.689115620722454 20.32661227005996 18.099181724601184 9 26.475057337933762 16.20175973793207 19.727104214932922 37.596078709201244 10-11 24.418834576229965 26.803504254751072 28.426284025451842 20.351377143567117 12-13 25.136869442281146 24.176126585531044 28.11598386592796 22.571020106259855 14-15 22.3088947064137 25.85653624290642 25.350141944256976 26.484427106422903 16-17 22.56679394357872 28.944429934430012 26.11749727981288 22.37127884217839 18-19 22.66779351142556 28.072048628092062 27.183931412269214 22.076226448213166 20-21 23.17904428821241 27.450893311497857 27.167270050107923 22.202792350181806 22-23 23.090089840027385 27.23420397664714 26.935724132826305 22.739982050499165 24-25 22.8248330088854 27.39320446352948 26.96426702082776 22.817695506757364 26-27 22.653624240450412 27.782160147656494 26.76461615815021 22.79959945374288 28-29 22.728681006570753 27.668588335063966 26.897788530473534 22.704942127891755 30-31 22.814071573889617 27.367670570620106 26.941270455574024 22.876987399916253 32-33 22.52086400922061 27.574672930055012 26.856639077093075 23.047823983631304 34-35 23.118935054525693 27.49584699603786 26.592178711876162 22.793039237560286 36-37 23.15541734054415 27.312327212877964 26.51686539774148 23.0153900488364 38-39 22.74172438578409 27.477489742470652 26.787080892590758 22.9937049791545 40-41 23.051606195482155 27.124809764987557 26.839863689029958 22.983720350500327 42-43 22.72984296598482 27.140451902037928 26.840886314436542 23.28881881754071 44-45 22.758539924621793 27.341981092796296 26.815438653629027 23.08404032895288 46-47 22.982787229987274 27.672396678572213 26.416326819781272 22.92848927165924 48-49 22.904508680788698 27.253468032978432 26.6630379958317 23.17898529040117 50-51 22.849867136719645 27.35212417350369 26.836479297542905 22.96152939223376 52-53 22.985301892777837 27.336364597652533 26.62506608861161 23.053267420958022 54-55 22.706470039611624 27.282373990411152 26.761770870123357 23.24938509985386 56-57 22.745002354057053 27.531444042834586 26.906012983397883 22.817540619710478 58-59 22.79794650008856 27.122324409631993 26.776866932254066 23.302862158025384 60-61 22.602270224915586 27.280078735390774 27.022280074770073 23.09537096492357 62-63 22.658139306186627 27.272811385416745 27.059794797721477 23.009254510675152 64-65 22.65891751568952 27.228678379392935 26.7893454020588 23.32305870285874 66-67 22.532757948855146 27.42214253878003 26.73962248588724 23.305477026477593 68-69 22.6650339795084 27.03707199129938 27.115454721614412 23.1824393075778 70-71 22.68071597211887 26.94375263860665 26.890167609846007 23.48536377942848 72-73 22.731137178103022 27.209276258534835 26.589459681521188 23.470126881840955 74-75 22.72186902635403 27.352402121096205 26.645140943347993 23.28058790920177 76-77 22.774871047628313 27.150157872809288 26.846361100478166 23.228609979084233 78-79 22.85923430776629 27.136945716961776 26.908434531836257 23.095385443435685 80-81 22.922173519154 27.16224836344209 26.784748916605665 23.130829200798246 82-83 22.828977890803543 27.216424805336764 26.727371018672603 23.22722628518709 84-85 22.870325014228808 27.08263334830453 26.82708318848985 23.219958448976808 86-87 22.865813763212888 27.00099001656196 27.00328992152566 23.129906298699495 88-89 22.88088903364437 27.08326283553427 26.870447604625785 23.165400526195572 90-91 22.852721367084847 27.156068364816434 26.860257537822946 23.130952730275776 92-93 22.88219313084274 26.806224924282134 26.976443067331466 23.335138877543656 94-95 22.681243860374472 26.822299014170444 27.014246574763057 23.482210550692027 96-97 22.78201769574631 26.64811794344446 27.217057071028833 23.352807289780404 98-99 22.74036663172796 26.70920994021648 26.937717017617835 23.612706410437728 100-101 22.637059297657263 26.804795499203365 26.9840543914992 23.57409081164017 102-103 22.600466638715574 26.516218946927644 27.11244428066209 23.77087013369469 104-105 22.483245250618058 26.749018189662284 27.070604693656144 23.69713186606352 106-107 22.588779210836186 26.727297145392072 26.86733361779481 23.81659002597694 108-109 22.63073314400083 26.72720971416925 26.756553344737693 23.88550379709223 110-111 22.65761019271002 26.929942037368264 26.88019029381022 23.532257476111496 112-113 22.71208297237488 26.88245835770932 27.08775857160116 23.31770009831464 114-115 22.64904167197588 27.0730905213002 26.75514588125943 23.52272192546449 116-117 22.560431260803924 26.966489362686435 26.92120507490677 23.551874301602872 118-119 22.45640046145082 27.052818769475063 26.791679074813324 23.6991016942608 120-121 22.563849976020403 27.100594281592922 26.906880373267427 23.428675369119247 122-123 22.535425364775314 27.26221762347294 26.830300599761387 23.372056411990364 124-125 22.60842071926151 27.23313597203721 26.758691647141585 23.3997516615597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 620.0 1 508.0 2 366.0 3 308.5 4 257.0 5 222.0 6 203.0 7 181.5 8 176.0 9 204.0 10 196.5 11 164.0 12 163.5 13 185.5 14 200.5 15 224.0 16 271.5 17 320.5 18 418.5 19 548.5 20 776.5 21 1236.0 22 1799.0 23 2581.0 24 3797.5 25 5426.0 26 7599.0 27 10564.5 28 13702.5 29 17222.0 30 21251.5 31 25406.5 32 30510.0 33 34045.5 34 35644.5 35 38998.0 36 45063.5 37 50234.0 38 52400.5 39 54984.0 40 57764.5 41 59911.5 42 62143.5 43 64597.5 44 68501.5 45 72606.5 46 72565.5 47 71422.0 48 71030.0 49 69916.0 50 68890.5 51 67322.0 52 66927.5 53 67821.5 54 69899.5 55 65893.0 56 57056.0 57 51898.5 58 49364.5 59 44088.5 60 35146.0 61 28275.0 62 23342.0 63 18024.0 64 13607.5 65 10119.5 66 6872.5 67 5150.0 68 3888.0 69 2565.0 70 1789.5 71 1220.0 72 864.5 73 647.0 74 489.5 75 366.5 76 258.0 77 144.5 78 78.0 79 60.0 80 40.5 81 28.5 82 26.0 83 16.0 84 12.0 85 12.5 86 14.5 87 14.5 88 10.0 89 7.5 90 9.0 91 9.0 92 5.0 93 3.5 94 2.5 95 2.0 96 1.5 97 1.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043882420490869815 2 0.00888222487044112 3 0.0032779639402818416 4 0.005762872088560011 5 0.02511343341344959 6 0.028972971601200794 7 0.0033308343264154194 8 5.287038613357809E-5 9 0.0 10-11 1.5861115840073425E-4 12-13 8.987965642708275E-4 14-15 3.4365750986825757E-4 16-17 2.1148154453431235E-4 18-19 5.287038613357809E-5 20-21 2.6435193066789044E-5 22-23 2.6435193066789044E-5 24-25 0.0 26-27 5.287038613357809E-5 28-29 3.172223168014685E-4 30-31 1.0574077226715618E-4 32-33 1.5861115840073425E-4 34-35 8.459261781372494E-4 36-37 5.287038613357809E-5 38-39 1.321759653339452E-4 40-41 3.965278960018357E-4 42-43 6.608798266697261E-4 44-45 0.0011895836880055069 46-47 1.321759653339452E-4 48-49 0.0 50-51 0.0 52-53 9.516669504044056E-4 54-55 0.0028550008512132166 56-57 1.321759653339452E-4 58-59 6.873150197365151E-4 60-61 0.0010309725296047728 62-63 8.459261781372494E-4 64-65 0.0014803708117401865 66-67 0.0023791673760110137 68-69 0.00304004720268074 70-71 0.0016654171632077097 72-73 0.0027492600789460605 74-75 0.0019826394800091783 76-77 0.001163148494938718 78-79 0.002352732182944225 80-81 0.0012953244602726631 82-83 5.5513905440257E-4 84-85 4.4939828213541377E-4 86-87 0.0018768987077420222 88-89 0.0056042609301592775 90-91 3.4365750986825757E-4 92-93 0.0011367133018719288 94-95 8.459261781372494E-4 96-97 1.5861115840073425E-4 98-99 5.5513905440257E-4 100-101 0.0010309725296047728 102-103 0.0010045373365379837 104-105 7.401854058700933E-4 106-107 0.0066087982666972606 108-109 0.0019297690938756002 110-111 0.0014275004256066083 112-113 0.0021941210245434908 114-115 0.0026963896928124827 116-117 0.0017711579354748657 118-119 0.04541566168874358 120-121 0.012001577652322227 122-123 0.004361806856020192 124-125 0.0025113433413449593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1891418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.71959964493503 #Duplication Level Percentage of deduplicated Percentage of total 1 81.33610970211082 54.26712676001348 2 10.933414377054856 14.589460599785534 3 3.485606010133419 6.976747125482433 4 1.5400263083303605 4.109997549378757 5 0.8208266454414664 2.738261258087483 6 0.4802271598117522 1.9224338304758604 7 0.30359704655078773 1.417911137947728 8 0.2077822758620083 1.1090520207061312 9 0.1476213825321984 0.8864315587422072 >10 0.6804625050061666 7.860962940991301 >50 0.047244398284059796 2.1856085713413935 >100 0.016907880844215562 1.8709962746093596 >500 1.743080380160547E-4 0.06501037243830834 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.6435193066789044E-5 0.0 24-25 0.0 0.0 0.0 2.643519306678904E-4 0.0 26-27 0.0 0.0 0.0 8.194909850704603E-4 0.0 28-29 0.0 0.0 0.0 0.0039652789600183565 0.0 30-31 0.0 0.0 0.0 0.013402642884862044 0.0 32-33 0.0 0.0 0.0 0.033942787897757135 0.0 34-35 0.0 0.0 0.0 0.06473978782056637 0.0 36-37 0.0 0.0 0.0 0.1138563765386604 0.0 38-39 0.0 0.0 0.0 0.18869440811074017 0.0 40-41 0.0 0.0 0.0 0.26818503366257485 0.0 42-43 0.0 0.0 0.0 0.34907672444694937 0.0 44-45 0.0 0.0 0.0 0.4470191147594027 0.0 46-47 0.0 0.0 0.0 0.5681980397775637 0.0 48-49 0.0 0.0 0.0 0.6955892351664201 0.0 50-51 0.0 0.0 0.0 0.833448767009725 0.0 52-53 0.0 0.0 0.0 0.9664706585218075 0.0 54-55 0.0 0.0 0.0 1.1111769053694105 0.0 56-57 0.0 0.0 0.0 1.2806000577344616 0.0 58-59 0.0 0.0 0.0 1.4707219662708084 0.0 60-61 0.0 0.0 0.0 1.681806982909119 0.0 62-63 0.0 0.0 0.0 1.889561165221014 0.0 64-65 0.0 0.0 0.0 2.0991922462406514 5.287038613357809E-5 66-67 0.0 0.0 0.0 2.3418673185937746 5.287038613357809E-5 68-69 0.0 0.0 0.0 2.617639252666518 5.287038613357809E-5 70-71 0.0 0.0 0.0 2.9132640167324197 1.0574077226715618E-4 72-73 0.0 0.0 0.0 3.2150217455898167 1.0574077226715618E-4 74-75 0.0 0.0 0.0 3.522119383446705 1.0574077226715618E-4 76-77 0.0 0.0 0.0 3.858269298483995 1.0574077226715618E-4 78-79 0.0 0.0 0.0 4.229049316438777 1.0574077226715618E-4 80-81 0.0 0.0 0.0 4.637288002969201 1.0574077226715618E-4 82-83 0.0 0.0 0.0 5.059484471438889 1.0574077226715618E-4 84-85 0.0 0.0 0.0 5.479116726181099 1.0574077226715618E-4 86-87 0.0 0.0 0.0 5.914213568867379 1.0574077226715618E-4 88-89 0.0 0.0 0.0 6.382856671555415 1.0574077226715618E-4 90-91 0.0 0.0 0.0 6.885257515789741 1.0574077226715618E-4 92-93 0.0 0.0 0.0 7.41076271876444 1.0574077226715618E-4 94-95 0.0 0.0 0.0 7.952049732000012 1.321759653339452E-4 96-97 0.0 0.0 0.0 8.501161562383356 1.5861115840073425E-4 98-99 0.0 0.0 0.0 9.06399325796836 1.5861115840073425E-4 100-101 0.0 0.0 0.0 9.677977052137603 1.5861115840073425E-4 102-103 0.0 0.0 0.0 10.333966368089973 1.5861115840073425E-4 104-105 0.0 0.0 0.0 10.985646747572456 1.5861115840073425E-4 106-107 0.0 2.6435193066789044E-5 0.0 11.617844389764716 1.5861115840073425E-4 108-109 0.0 5.287038613357809E-5 0.0 12.281843569216324 1.5861115840073425E-4 110-111 0.0 5.287038613357809E-5 0.0 12.983618639560373 1.5861115840073425E-4 112-113 0.0 5.287038613357809E-5 0.0 13.739030716636936 1.5861115840073425E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1735 0.0 47.00584 1 GTATCAA 2440 0.0 45.622856 1 ATCAACG 2590 0.0 41.58945 3 CAACGCA 2645 0.0 40.724644 5 TCAACGC 2810 0.0 38.33334 4 AACGCAG 2885 0.0 37.5411 6 GTACATG 6465 0.0 36.463417 1 TATCAAC 3150 0.0 34.384697 2 TACATGG 6975 0.0 33.360836 2 ACATGGG 6850 0.0 32.927063 3 ACGCAGA 3450 0.0 31.042341 7 CGCAGAG 3515 0.0 30.466688 8 CATGGGG 3735 0.0 27.883791 4 CATGGGA 3935 0.0 26.164099 4 GCAGAGT 4490 0.0 23.188347 9 ATGGGGA 2145 0.0 21.363276 5 GAGTACA 5385 0.0 20.451105 1 CAGAGTA 4325 0.0 20.221312 10-11 TAGACCG 180 5.777685E-4 19.837324 5 AGAGTAC 4255 0.0 19.645126 10-11 >>END_MODULE