FastQCFastQC Report
Thu 26 May 2016
SRR1364409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891418
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.18562792571499268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.1751595892605442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29500.067.015391
GTATCAA50050.057.2267951
ATCAACG62000.045.5124473
TCAACGC63800.044.135094
CAACGCA64750.043.487555
TATCAAC65150.043.3119242
AACGCAG67150.042.182496
ACGCAGA79800.035.1918037
CGCAGAG80000.034.955088
GTACATG68150.033.1155361
TACATGG72850.030.480552
ACATGGG70200.030.3591423
GTGGTAT14050.030.0912691
GCAGAGT93300.029.4620349
GAGTACT56200.026.0436112-13
CAGAGTA89300.025.85131610-11
TGGTATC15500.025.7327772
GTACCGT1901.1676129E-625.0557676
AGAGTAC86900.024.34009410-11
CATGGGG38150.024.0308844
Produced by FastQC (version 0.11.5)