FastQCFastQC Report
Thu 26 May 2016
SRR1364408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386718
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.061.0462841
GTATCAA25300.051.991891
ATCAACG30600.041.8136673
TCAACGC30850.041.474824
CAACGCA32500.039.3691755
GTACATG78950.038.674891
TATCAAC33400.038.4865072
AACGCAG33400.038.3055656
TACATGG82150.036.8008042
ACATGGG85800.034.7497443
TTGTGCG902.3940677E-433.0536239
ACGCAGA40500.031.2918917
CATGGGA57500.031.2564964
CGCAGAG40600.031.0671488
CATGGGG34500.028.6345394
ATGGGAT23700.025.361465
GCAGAGT49250.025.248279
GAGTACA58650.023.8486441
CAGAGTA47150.022.83959810-11
ATGGGAG23950.022.6118985
Produced by FastQC (version 0.11.5)