FastQCFastQC Report
Thu 26 May 2016
SRR1364408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386718
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45550.3284734170898481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.3036666431098464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.17033023296733726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.082.0446551
GTATCAA54550.053.5846141
ATCAACG69700.041.757593
TCAACGC71300.040.737064
TATCAAC71250.040.682112
CAACGCA71650.040.5380635
AACGCAG74350.039.0532576
TGGTATC10250.038.324762
GTACATG75750.036.466051
GTGGTAT11600.034.89831
TACATGG78850.034.4209252
ACGCAGA86300.033.644327
CGCAGAG86450.033.5859458
ACATGGG80950.032.498613
CATGGGG32750.030.7138864
GCAGAGT96800.029.8719569
GAGTACT58200.027.7044212-13
CAGAGTA94000.027.50202610-11
CATGGGA55500.026.4888654
GTACTTT61250.026.13057314-15
Produced by FastQC (version 0.11.5)