FastQCFastQC Report
Thu 26 May 2016
SRR1364407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303883
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.071.117551
GTATCAA30650.059.041041
TCAACGC39750.044.0187534
ATCAACG40200.043.3779643
CAACGCA40950.042.7288255
TATCAAC43200.041.3299672
AACGCAG42300.041.2212836
GTACATG95950.039.0846671
TACATGG100250.036.748012
ACATGGG99100.035.252673
CCGCAAT700.002789837533.9982229
ACGCAGA52100.033.2327467
CGCAGAG51800.033.1942148
CATGGGA77800.032.893984
TTAGACG800.005355794429.7575743
ATGGGAT32100.029.479475
CATGGGG24350.028.5966034
GCAGAGT60750.027.1286249
ATGGGAG28750.027.1182085
CGTATAG1556.8085883E-626.8829671
Produced by FastQC (version 0.11.5)