##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364407_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1303883 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.91479373532748 31.0 31.0 34.0 27.0 34.0 2 30.85198748660731 33.0 31.0 34.0 27.0 34.0 3 31.000729359919564 33.0 31.0 34.0 27.0 34.0 4 34.24731053323036 37.0 35.0 37.0 30.0 37.0 5 34.43907160381721 37.0 35.0 37.0 31.0 37.0 6 34.445172611346266 37.0 35.0 37.0 31.0 37.0 7 34.37579982253009 37.0 35.0 37.0 31.0 37.0 8 34.2851390807304 37.0 35.0 37.0 30.0 37.0 9 35.695791723643914 39.0 37.0 39.0 30.0 39.0 10-11 35.65554961603149 39.0 37.0 39.0 30.0 39.0 12-13 35.64879594258073 39.0 37.0 39.0 30.0 39.0 14-15 36.93093206982528 40.0 38.0 41.0 30.0 41.0 16-17 36.96960808600158 40.0 38.0 41.0 30.0 41.0 18-19 37.011283604433835 40.0 38.0 41.0 30.0 41.0 20-21 37.07925826166918 40.0 38.0 41.0 30.0 41.0 22-23 37.09460588104914 40.0 38.0 41.0 30.0 41.0 24-25 37.159525432880095 40.0 38.0 41.0 30.0 41.0 26-27 37.17524808590955 40.0 37.0 41.0 30.0 41.0 28-29 37.210706405406 40.0 37.0 41.0 30.0 41.0 30-31 37.119503820511504 40.0 37.0 41.0 30.0 41.0 32-33 37.081281449332494 40.0 37.0 41.0 30.0 41.0 34-35 37.07072145276838 40.0 37.0 41.0 30.0 41.0 36-37 36.99281645669129 40.0 37.0 41.0 30.0 41.0 38-39 36.89512939427847 40.0 37.0 41.0 30.0 41.0 40-41 36.82672179942526 40.0 37.0 41.0 30.0 41.0 42-43 36.684100490611506 40.0 36.5 41.0 30.0 41.0 44-45 36.5558493361751 40.0 36.0 41.0 29.5 41.0 46-47 36.445216326925035 40.0 36.0 41.0 29.0 41.0 48-49 36.304140785637976 40.0 36.0 41.0 29.0 41.0 50-51 36.193326778552986 40.0 35.0 41.0 29.0 41.0 52-53 36.03507369909724 40.0 35.0 41.0 29.0 41.0 54-55 35.85935011040101 39.0 35.0 41.0 28.0 41.0 56-57 35.67392856567652 39.0 35.0 41.0 28.0 41.0 58-59 35.46132283341373 39.0 35.0 41.0 28.0 41.0 60-61 35.21446326089074 38.5 34.0 40.5 28.0 41.0 62-63 34.93958391972286 38.0 34.0 40.0 28.0 41.0 64-65 34.59040304996691 37.0 34.0 40.0 27.0 41.0 66-67 34.26961736597532 37.0 34.0 40.0 26.0 41.0 68-69 33.93111421807018 36.0 33.5 39.0 26.0 41.0 70-71 33.565161137924186 36.0 33.0 39.0 26.0 41.0 72-73 33.20466636960525 35.0 33.0 38.5 26.0 40.0 74-75 32.842362006407015 35.0 33.0 37.5 25.0 40.0 76-77 32.60218248109685 35.0 33.0 37.0 25.5 39.0 78-79 32.42650184103942 35.0 33.0 37.0 25.0 39.0 80-81 32.150846740083274 35.0 33.0 36.0 25.0 39.0 82-83 31.853617234061645 35.0 33.0 36.0 24.5 37.0 84-85 31.593364205223935 35.0 32.5 35.5 24.0 37.0 86-87 31.39058067326593 35.0 32.0 35.0 23.5 36.5 88-89 31.133306822774742 35.0 32.0 35.0 22.0 36.0 90-91 31.00344931255335 35.0 32.0 35.0 21.5 36.0 92-93 30.857218784200732 35.0 32.0 35.0 20.0 36.0 94-95 30.758833039467497 35.0 32.0 35.0 20.0 35.5 96-97 30.65498974984719 35.0 32.0 35.0 19.5 35.0 98-99 30.52692074365568 35.0 32.0 35.0 18.5 35.0 100-101 30.453147636712803 35.0 32.0 35.0 18.0 35.0 102-103 29.44688978995815 33.5 29.5 34.5 16.0 35.0 104-105 30.149776475343263 34.0 31.0 35.0 17.0 35.0 106-107 30.49284406653051 35.0 32.0 35.0 18.0 35.0 108-109 30.54017346648434 35.0 32.0 35.0 17.5 35.0 110-111 30.472603753557642 35.0 32.0 35.0 15.0 35.0 112-113 30.390378201111602 35.0 32.0 35.0 10.5 35.0 114-115 30.29007165520219 35.0 32.0 35.0 4.5 35.0 116-117 30.164309604466045 35.0 32.0 35.0 2.0 35.0 118-119 29.999266421910555 35.0 32.0 35.0 2.0 35.0 120-121 29.823576195103396 35.0 31.5 35.0 2.0 35.0 122-123 29.65261338632377 35.0 31.0 35.0 2.0 35.0 124-125 28.847176088652127 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2957377055550694 1101 2 0.22218863132533073 1101 3 0.341134477313517 1101 4 0.4131327679281398 1101 5 0.3038135534996229 1101 6 0.23405327535521536 1101 7 0.3360323811132844 1101 8 0.23987311870001804 1101 9 0.3473255292399955 1101 10-11 0.4734141444958766 1101 12-13 0.5714820594017738 1101 14-15 0.6277000648339666 1101 16-17 0.48189982022443445 1101 18-19 0.3328077525573292 1101 20-21 0.48024583609365834 1101 22-23 0.5413455028708185 1101 24-25 0.4596509259778472 1101 26-27 0.5111906155093067 1101 28-29 0.41872221889700967 1101 30-31 0.5240326832807654 1101 32-33 0.49114777844003044 1101 34-35 0.4862894747381503 1101 36-37 0.5246828023316965 1101 38-39 0.5536752233407256 1101 40-41 0.6065718947906262 1101 42-43 0.7014841706345365 1101 44-45 0.5828773243116743 1101 46-47 0.6183049909011231 1101 48-49 0.5966930327851045 1101 50-51 0.5098717097838232 1101 52-53 0.4705611090104611 1101 54-55 0.5986379324425499 1101 56-57 0.42691156481011205 1101 58-59 0.4598727569956864 1101 60-61 0.40094750550936453 1101 62-63 0.40261627722642856 1101 64-65 0.42504116435239325 1101 66-67 0.46594127257885276 1101 68-69 0.43397516745263687 1101 70-71 0.37522670556119664 1101 72-73 0.4730814919399222 1101 74-75 0.43637490768131215 1101 76-77 0.39592774018278476 1101 78-79 0.3709879173964765 1101 80-81 0.39100765873971 1101 82-83 0.30193416120369676 1101 84-85 0.25717900394067783 1101 86-87 0.23990735944085273 1101 88-89 0.28171646479008317 1101 90-91 0.2115697012908413 1101 92-93 0.17723521538015774 1101 94-95 0.25196013883891766 1101 96-97 0.15923230528580845 1101 98-99 0.12268776858436681 1101 100-101 0.11161013675098985 1101 102-103 -0.11224831628698695 1101 104-105 0.07496259630752888 1101 106-107 0.1803883277704159 1101 108-109 0.22464170019352991 1101 110-111 0.14857279763004172 1101 112-113 0.18658525723441244 1101 114-115 0.10462295469103822 1101 116-117 0.0776603503016382 1101 118-119 0.13063545748577354 1101 120-121 0.024218686598938888 1101 122-123 -0.15299308457466054 1101 124-125 -0.18515912395975676 1102 1 0.35883109050902107 1102 2 0.322229400433649 1102 3 0.4140713842323578 1102 4 0.49659126424676714 1102 5 0.3145821271398148 1102 6 0.33785956680026885 1102 7 0.2339619666050723 1102 8 0.30372689916899986 1102 9 0.4618712301344914 1102 10-11 0.32491038899873104 1102 12-13 0.34292913186385476 1102 14-15 0.3820528203031657 1102 16-17 0.40301235511513767 1102 18-19 0.42476624340767444 1102 20-21 0.3385508558584718 1102 22-23 0.3825309907694958 1102 24-25 0.4220722586112444 1102 26-27 0.4475819965187995 1102 28-29 0.3976826856212696 1102 30-31 0.504860417697067 1102 32-33 0.4858058708346178 1102 34-35 0.4929657959671303 1102 36-37 0.34959133006259435 1102 38-39 0.4140109517832258 1102 40-41 0.2525810487009892 1102 42-43 0.22903096397100597 1102 44-45 0.2350328314222736 1102 46-47 0.14745733369701952 1102 48-49 0.31732378132051764 1102 50-51 0.24984165851560647 1102 52-53 0.21398701434593193 1102 54-55 0.17351334666520302 1102 56-57 0.10186845649066356 1102 58-59 0.15790292856618748 1102 60-61 0.07325501273662383 1102 62-63 0.09795377374798875 1102 64-65 0.05508322762167239 1102 66-67 0.13677568437966414 1102 68-69 0.07591758761871148 1102 70-71 -0.028268835355554245 1102 72-73 0.02740511359773734 1102 74-75 0.00649458415322357 1102 76-77 0.10936966759554423 1102 78-79 0.17738523453396482 1102 80-81 0.14256991760349536 1102 82-83 0.22491031826258734 1102 84-85 0.1721707042980576 1102 86-87 0.16855260987011533 1102 88-89 0.13775077889084741 1102 90-91 0.15805872765648488 1102 92-93 0.23271203774714877 1102 94-95 0.16917682391547828 1102 96-97 0.11699860776464277 1102 98-99 0.18581882395136873 1102 100-101 0.0702948074447427 1102 102-103 -0.09337300899970558 1102 104-105 0.10331429092928701 1102 106-107 0.10133878874690794 1102 108-109 0.15700629632417318 1102 110-111 0.12707863715165146 1102 112-113 0.12732073559397605 1102 114-115 0.11546580587954125 1102 116-117 0.25125305658056263 1102 118-119 0.32496056165661713 1102 120-121 0.14018234437174115 1102 122-123 0.2585642285284493 1102 124-125 0.2983286276877166 1103 1 0.2357410956753654 1103 2 0.24821000788455905 1103 3 0.24211828787336032 1103 4 0.33005073142353325 1103 5 0.11818507836927239 1103 6 0.1335517784437812 1103 7 0.23059053298656096 1103 8 0.2022560031165952 1103 9 0.21929649053886635 1103 10-11 0.31440619240174783 1103 12-13 0.10171993480105357 1103 14-15 0.18649000927888437 1103 16-17 0.17046586103102612 1103 18-19 0.25438316554830465 1103 20-21 0.3195093339281243 1103 22-23 0.2996665002594838 1103 24-25 0.39267974581939313 1103 26-27 0.5242099700409781 1103 28-29 0.3149685272939635 1103 30-31 0.4239555389488956 1103 32-33 0.487917175935749 1103 34-35 0.5150913962371888 1103 36-37 0.39626569180114757 1103 38-39 0.2871033664508076 1103 40-41 0.2799852142031156 1103 42-43 0.3346052934047776 1103 44-45 0.3049613434784888 1103 46-47 0.2320023988691986 1103 48-49 0.26923502665174937 1103 50-51 0.2749356388157338 1103 52-53 0.34138915323714514 1103 54-55 0.32425690113505823 1103 56-57 0.18162769511411625 1103 58-59 0.3395055664271496 1103 60-61 0.1691221783730441 1103 62-63 0.15925058694769945 1103 64-65 0.19745642529911578 1103 66-67 0.030202266013368728 1103 68-69 0.07740971771381311 1103 70-71 0.2517239474897579 1103 72-73 0.21794056839947729 1103 74-75 0.09855852705482704 1103 76-77 0.021185928395567544 1103 78-79 0.14315229769042048 1103 80-81 0.20105443006140433 1103 82-83 0.181529955456881 1103 84-85 0.07559649343615149 1103 86-87 0.13336489730282963 1103 88-89 0.24370290489643764 1103 90-91 0.13333180591512672 1103 92-93 0.09329345729432248 1103 94-95 0.0607617606912676 1103 96-97 0.052490372464831125 1103 98-99 -0.005525611580019074 1103 100-101 0.02461741728403055 1103 102-103 -0.2087767038441264 1103 104-105 0.06352813770298482 1103 106-107 0.08019367493606921 1103 108-109 0.06320264406882714 1103 110-111 0.0905042085046901 1103 112-113 0.03029189843307023 1103 114-115 -0.16036062790231043 1103 116-117 -0.26184565570481055 1103 118-119 -0.3303442285256324 1103 120-121 -0.24735844495388548 1103 122-123 -0.44581751600242825 1103 124-125 -0.5033352803682725 1104 1 0.3388477618677648 1104 2 0.3884378074789474 1104 3 0.41174011657189524 1104 4 0.39697004817599435 1104 5 0.39959070611212155 1104 6 0.2990161119015653 1104 7 0.4017640003023004 1104 8 0.36093317798038527 1104 9 0.418188273268008 1104 10-11 0.5107320297894589 1104 12-13 0.5533492813847403 1104 14-15 0.5606515572652739 1104 16-17 0.4561537592861029 1104 18-19 0.4527821489729362 1104 20-21 0.5191401055546478 1104 22-23 0.4992168891634208 1104 24-25 0.4721008165676679 1104 26-27 0.4388544417402329 1104 28-29 0.42256402238851365 1104 30-31 0.38609653696621393 1104 32-33 0.47667311746529606 1104 34-35 0.3579186666499865 1104 36-37 0.35482965866329863 1104 38-39 0.32385759613644893 1104 40-41 0.44348997372853916 1104 42-43 0.2595943493111008 1104 44-45 0.33462522886560464 1104 46-47 0.3302667762433913 1104 48-49 0.21656053662691477 1104 50-51 0.257236315571042 1104 52-53 0.2635113187308775 1104 54-55 0.3049593060908933 1104 56-57 0.2593372020375355 1104 58-59 0.3948608342542599 1104 60-61 0.23995238065462843 1104 62-63 0.3171369582671488 1104 64-65 0.3663036950453247 1104 66-67 0.3910883412891266 1104 68-69 0.2672185328696841 1104 70-71 0.2018001150207951 1104 72-73 0.04881938122228746 1104 74-75 0.053929031188935994 1104 76-77 0.16341965502540745 1104 78-79 0.3481289404878041 1104 80-81 0.266129565500691 1104 82-83 0.27018481641297853 1104 84-85 0.29937569133981157 1104 86-87 0.2853028071111723 1104 88-89 0.3078145679813602 1104 90-91 0.294003144489114 1104 92-93 0.17207286665776422 1104 94-95 0.15257367408294442 1104 96-97 0.20105486867662137 1104 98-99 0.2438045090584815 1104 100-101 0.18485855424862763 1104 102-103 0.018494241106591147 1104 104-105 0.10546439224341597 1104 106-107 0.12818790194415897 1104 108-109 0.1950538357636944 1104 110-111 0.12117805938885695 1104 112-113 0.0710507001311349 1104 114-115 0.08941593893760214 1104 116-117 0.0252137902593752 1104 118-119 0.08884172696026482 1104 120-121 0.07088165515761347 1104 122-123 -0.13013663933220698 1104 124-125 -0.2832102754318768 1105 1 0.09208709072997223 1105 2 0.08617352725995175 1105 3 0.04037983287488345 1105 4 0.15369831536947487 1105 5 0.15454746533408326 1105 6 0.19545961651441246 1105 7 0.23121733158973967 1105 8 0.14232509841224328 1105 9 0.14498844503768282 1105 10-11 0.10618887106021191 1105 12-13 0.1651492826634211 1105 14-15 0.2049807071758707 1105 16-17 0.08017250370519236 1105 18-19 0.07240861919136421 1105 20-21 0.14046415991570171 1105 22-23 0.08014816424424254 1105 24-25 0.19081345506496206 1105 26-27 0.1776963390492483 1105 28-29 0.18299181092853445 1105 30-31 0.15501957200424243 1105 32-33 0.17194391044021984 1105 34-35 0.03459448655181774 1105 36-37 0.013002410792836372 1105 38-39 -0.023794583058105445 1105 40-41 0.08307308062852314 1105 42-43 0.07789996720242698 1105 44-45 0.03370263284188724 1105 46-47 0.09157725525915339 1105 48-49 -0.011164149190150852 1105 50-51 -0.039213289422640685 1105 52-53 0.016412995509796247 1105 54-55 -0.05533224949023463 1105 56-57 -0.09919936188699552 1105 58-59 -0.10047770225106234 1105 60-61 -0.06214007661074561 1105 62-63 -0.18504226293000414 1105 64-65 -0.10320798678438337 1105 66-67 -0.1722201589655512 1105 68-69 -0.2197555556961177 1105 70-71 -0.29769187509924677 1105 72-73 -0.3410146671076504 1105 74-75 -0.25357598231182266 1105 76-77 -0.18515210996127962 1105 78-79 -0.03134684766008178 1105 80-81 -0.036623677557663825 1105 82-83 0.0018042890417149238 1105 84-85 -0.049556102672969615 1105 86-87 -0.11645788533799717 1105 88-89 -0.09418814175985446 1105 90-91 -0.12090666577572406 1105 92-93 -0.167733663360373 1105 94-95 -0.2811004014829237 1105 96-97 -0.18510802907353607 1105 98-99 -0.23645276717741837 1105 100-101 -0.32945869325953936 1105 102-103 -0.3711887628594539 1105 104-105 -0.2090303523717303 1105 106-107 -0.20318105548210852 1105 108-109 -0.1612626157646524 1105 110-111 -0.14855867055127803 1105 112-113 -0.27133663728972124 1105 114-115 -0.26676518536524796 1105 116-117 -0.20239368300017802 1105 118-119 -0.2319673114065992 1105 120-121 -0.4770705349057458 1105 122-123 -0.4698998806217176 1105 124-125 -0.5543845907507041 1106 1 0.19452562178690513 1106 2 0.26011835188099397 1106 3 0.225189077414214 1106 4 0.29700397735329886 1106 5 0.246482161796429 1106 6 0.23904675743814607 1106 7 0.24184030230449594 1106 8 0.2439574750560567 1106 9 0.38785430941524623 1106 10-11 0.4277642635740193 1106 12-13 0.38535254476924763 1106 14-15 0.35818552175770435 1106 16-17 0.47290459145714436 1106 18-19 0.4067449462830197 1106 20-21 0.2545907386016637 1106 22-23 0.40809945716237195 1106 24-25 0.2924075757768563 1106 26-27 0.45402542160582016 1106 28-29 0.27559697900047553 1106 30-31 0.35333342525819234 1106 32-33 0.45156432687656434 1106 34-35 0.296633323868015 1106 36-37 0.3038123563820534 1106 38-39 0.27672469157765534 1106 40-41 0.2814564299525699 1106 42-43 0.15319399514699938 1106 44-45 0.09938542047883914 1106 46-47 0.11503065662185463 1106 48-49 0.0497343966078887 1106 50-51 -2.5908746520997283E-4 1106 52-53 0.09262926939791072 1106 54-55 0.03670318318038568 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0.45593096044582637 2204 32-33 0.5088769485507925 2204 34-35 0.45943792902811964 2204 36-37 0.3277020440009295 2204 38-39 0.4012221909622937 2204 40-41 0.44949708313344416 2204 42-43 0.4878536709061265 2204 44-45 0.3150353846758449 2204 46-47 0.3039553640442634 2204 48-49 0.3754613652131411 2204 50-51 0.4700549159808389 2204 52-53 0.48308413524856775 2204 54-55 0.3344959845409008 2204 56-57 0.46692718132288036 2204 58-59 0.30902757597402086 2204 60-61 0.4089057276476211 2204 62-63 0.2517319811208125 2204 64-65 -2.2915142580314694 2204 66-67 -0.34450835900133114 2204 68-69 0.13365516178464532 2204 70-71 0.3165213115173202 2204 72-73 0.3060811908275838 2204 74-75 0.25720523973147635 2204 76-77 0.30736779520959345 2204 78-79 0.15840422587722003 2204 80-81 0.16958941959745744 2204 82-83 0.2634577601456698 2204 84-85 0.3492473505386968 2204 86-87 0.29000905865914106 2204 88-89 0.35153895611096075 2204 90-91 0.3475861325781864 2204 92-93 0.34983768213971445 2204 94-95 0.32400672859401425 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-1.2908575215496363 2216 96-97 -1.275648900341281 2216 98-99 -1.2878874311610318 2216 100-101 -1.3205733394769759 2216 102-103 -1.444563520387419 2216 104-105 -1.4734433887561345 2216 106-107 -1.3867373724451824 2216 108-109 -1.2053610276861164 2216 110-111 -1.2427941811475307 2216 112-113 -1.19530760409598 2216 114-115 -1.2642119774828693 2216 116-117 -1.3207864333129002 2216 118-119 -1.331025749433465 2216 120-121 -1.1727903369397907 2216 122-123 -1.1499913401674107 2216 124-125 -1.1897576351117571 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22682.0 3 4616.0 4 3115.0 5 2538.0 6 3039.0 7 3648.0 8 3630.0 9 3694.0 10 4262.0 11 4985.0 12 5638.0 13 5801.0 14 5674.0 15 5397.0 16 4942.0 17 4217.0 18 3940.0 19 3646.0 20 3612.0 21 3630.0 22 4247.0 23 5552.0 24 9046.0 25 13721.0 26 16687.0 27 14458.0 28 15379.0 29 19387.0 30 22619.0 31 27541.0 32 36301.0 33 48683.0 34 68260.0 35 106039.0 36 207510.0 37 402315.0 38 182885.0 39 547.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.407726138557344 19.712333770167785 12.843700223123172 23.0362398681517 2 15.151914928867058 21.374244874040848 39.76449581056578 23.70934438652631 3 18.131066192683946 27.727912443580333 29.40709977029478 24.73392159344094 4 11.683459770564554 17.46996684330297 38.10113291133901 32.74544047479347 5 12.256012053257724 40.96826507347639 33.97044067422143 12.805282199044454 6 30.113613260736642 38.20041627763708 18.27156553687617 13.414404924750107 7 27.779963630478914 34.5445258195222 21.240505921359922 16.435004628638968 8 26.482457768417696 35.8523240599993 20.23534338229993 17.42987478928308 9 27.487972463787013 16.913710816077824 19.592478773018744 36.00583794711642 10-11 23.86298141357171 28.949333202442094 27.43949507681488 19.748190307171317 12-13 24.649313198401682 26.735525780944418 27.15761540644389 21.457545614210005 14-15 22.086750380978604 27.42965413201685 25.084076053069342 25.399519433935204 16-17 21.588988250452495 30.786230327944292 26.106313464429242 21.51846795717397 18-19 21.87640445991278 29.3543050674831 27.553451922796697 21.215838549807422 20-21 22.927287187577413 28.440626958093635 27.238640276773303 21.393445577555656 22-23 22.797615121613767 28.61712179476364 26.999529098492044 21.585733985130556 24-25 22.019153559023316 28.799094704049367 27.327566967281573 21.854184769645745 26-27 21.707975987100063 29.01925595289453 27.04760590007152 22.22516215993389 28-29 21.995215813737317 28.897236630086077 27.124487778390478 21.983059777786128 30-31 21.90835593571962 28.55386014756709 27.160213562352098 22.37757035436119 32-33 21.678832256740925 28.84911025341624 26.971448731001452 22.500608758841384 34-35 22.503793504507914 28.694846547613707 26.82721905154143 21.97414089633695 36-37 22.511746657959332 28.166248236515358 26.789129226850754 22.532875878674556 38-39 21.99541215801135 28.54995969724231 26.83481084546826 22.61981729927808 40-41 22.218387498801647 28.15239190873358 27.308407631099605 22.32081296136516 42-43 21.976458587783696 28.171312762559225 27.111469871479 22.74075877817808 44-45 21.864900227477364 28.42462338921331 27.323053135828374 22.387423247480953 46-47 22.474984335250937 28.66652912876385 26.60307711658178 22.255409419403428 48-49 21.89885902339397 28.675080509524243 26.602540258596825 22.82352020848496 50-51 22.075063483456724 28.608280037395993 27.032716892543274 22.283939586604014 52-53 22.35244987903323 28.31799630408081 26.902518309908846 22.427035506977113 54-55 21.724234595365292 28.5835302202385 27.185003641177403 22.5072315432188 56-57 21.937354712584966 28.809761013466716 26.894210437915728 22.358673836032583 58-59 21.878379352606157 28.001670406803107 27.05675548404217 23.06319475654856 60-61 21.363493258346395 28.535710765976557 27.357872305668156 22.742923670008892 62-63 21.616829246406667 28.486324971833916 27.482316145820953 22.414529635938468 64-65 21.502393074751133 28.493829698055745 27.183074340816333 22.820702886376786 66-67 21.605894838564947 28.60940942322847 26.883140539286458 22.901555198920125 68-69 21.972008899844536 28.087392423781083 27.296461307074548 22.644137369299834 70-71 21.7845626179915 28.132356792878348 27.1116907599736 22.971389829156553 72-73 21.800564023232788 28.219990535699356 26.928121167590984 23.051324273476872 74-75 21.84329605939288 28.548617182826376 26.866803184390964 22.74128357338978 76-77 22.01485196923767 28.286002987266727 27.161540570648473 22.537604472847125 78-79 22.206878832385506 28.14626626427018 27.032837240337308 22.614017663007004 80-81 22.174223991318136 28.40507183436967 26.899742496841128 22.520961677471064 82-83 22.144108906145142 28.331665228645385 26.93839516824849 22.585830696960983 84-85 22.11829273577117 28.308952810295157 26.99245441761886 22.580300036314817 86-87 22.268243887642374 28.29069501236526 26.915081399036474 22.525979700955897 88-89 22.29277672401958 28.217194521548183 26.912583918226655 22.577444836205583 90-91 22.142187437086687 28.408842088309676 26.86812986649436 22.580840608109273 92-93 22.28586197825392 27.8703656124436 27.055251800988906 22.78852060831358 94-95 22.073302468839913 27.68336450332721 27.32305292354346 22.920280104289418 96-97 22.511760106482313 27.49496981505562 27.192257560808002 22.80101251765406 98-99 22.124598648106087 27.694536552405925 27.028061366335486 23.1528034331525 100-101 21.9416457097103 27.878235673533187 27.279783228759463 22.90033538799705 102-103 21.897330176574485 27.630153029177013 27.275976344249308 23.196540449999194 104-105 21.73812673760905 28.000997028089348 27.070731473492476 23.190144760809126 106-107 21.809776989651755 28.034447106755966 26.849982665999896 23.305793237592383 108-109 21.91107687080595 28.07686275291798 26.711029933125307 23.30103044315076 110-111 21.942447284631736 28.390926824891068 26.75467676689045 22.91194912358675 112-113 21.736761211727043 28.62113819142882 26.987563062848867 22.654537533995267 114-115 21.878097819480217 28.748685134416363 26.598690560693584 22.774526485409833 116-117 21.71726719926618 28.670831491042147 26.81092589560278 22.800975414088896 118-119 21.58588584464024 28.924795455086056 26.549634416489738 22.939684283783958 120-121 21.577513014651117 29.054408029926606 26.626668395050174 22.741410560372103 122-123 21.749317871105923 29.06822055916354 26.571484998916645 22.610976570813897 124-125 21.76539885431343 29.00670093960411 26.606496007583715 22.62140419849875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2205.0 1 1804.5 2 1299.5 3 1028.0 4 741.0 5 612.0 6 545.5 7 504.5 8 512.5 9 496.5 10 444.5 11 402.5 12 395.0 13 381.5 14 421.5 15 461.0 16 497.0 17 557.5 18 724.0 19 926.5 20 1133.0 21 1532.5 22 2213.0 23 2998.0 24 3845.0 25 5220.5 26 6947.0 27 8982.5 28 11048.0 29 13601.0 30 17032.5 31 20491.0 32 25218.0 33 28380.0 34 29881.0 35 31783.0 36 35740.5 37 40947.0 38 42550.5 39 42782.5 40 42655.5 41 42926.5 42 43210.5 43 43919.5 44 45880.0 45 48778.0 46 48378.0 47 45699.0 48 43687.5 49 41746.0 50 41900.0 51 41321.5 52 40757.0 53 41653.0 54 42360.5 55 40112.5 56 36323.0 57 33137.5 58 31181.5 59 28046.5 60 22355.5 61 17995.0 62 14708.5 63 10477.5 64 7307.5 65 5795.5 66 4350.0 67 3352.5 68 2540.5 69 1661.0 70 1036.5 71 660.0 72 448.5 73 357.5 74 280.5 75 215.5 76 136.0 77 77.5 78 62.0 79 46.0 80 34.0 81 24.5 82 14.5 83 11.0 84 12.0 85 10.5 86 10.0 87 9.0 88 7.5 89 4.5 90 4.0 91 3.5 92 2.0 93 3.0 94 2.5 95 1.5 96 2.5 97 2.5 98 1.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04348549678153638 2 0.00974013772708134 3 0.002914371918339299 4 0.005675355840976529 5 0.025692489280096452 6 0.032671643084540555 7 0.0033745359054455043 8 7.669399785103417E-5 9 0.0 10-11 1.1504099677655127E-4 12-13 9.970219720634443E-4 14-15 3.067759914041367E-4 16-17 2.3008199355310254E-4 18-19 7.669399785103417E-5 20-21 0.0 22-23 7.669399785103417E-5 24-25 0.0 26-27 3.834699892551709E-5 28-29 3.067759914041367E-4 30-31 1.1504099677655127E-4 32-33 3.834699892551709E-5 34-35 7.285929795848247E-4 36-37 1.1504099677655127E-4 38-39 1.5338799570206835E-4 40-41 6.135519828082734E-4 42-43 8.052869774358588E-4 44-45 0.001303797963467581 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.586749731379272E-4 54-55 0.0035662709000730893 56-57 1.5338799570206835E-4 58-59 6.902459806593076E-4 60-61 8.819809752868931E-4 62-63 7.669399785103418E-4 64-65 0.0012654509645420638 66-67 0.0023391669344565423 68-69 0.003067759914041367 70-71 0.002185778938754474 72-73 0.0027609839226372303 74-75 0.0017639619505737862 76-77 0.0011887569666910298 78-79 0.002147431939828957 80-81 0.0011887569666910298 82-83 6.135519828082734E-4 84-85 5.752049838827564E-4 86-87 0.002185778938754474 88-89 0.0064422958194868705 90-91 4.2181698818068804E-4 92-93 0.0012271039656165468 94-95 8.819809752868931E-4 96-97 3.451229903296538E-4 98-99 6.518989817337905E-4 100-101 0.0015338799570206835 102-103 0.0010737159699144784 104-105 6.135519828082734E-4 106-107 0.006672377813039973 108-109 0.0020323909430524055 110-111 0.0013421449623930981 112-113 0.0019173499462758544 114-115 0.003259494908668953 116-117 0.0020323909430524055 118-119 0.04785705465904533 120-121 0.012271039656165468 122-123 0.004639986869987568 124-125 0.0027609839226372303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1303883.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.015032166683056 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21006456396812 43.577442499117474 2 10.980985541726563 12.082385455999367 3 3.9355677369215023 6.495461569226895 4 1.8031216581630114 3.9679518409712378 5 1.0079487803310385 2.7726167286140515 6 0.6444238040381095 2.127179778487971 7 0.4140045767065147 1.5943532573264037 8 0.31463431997223035 1.3847693787211761 9 0.21809640719659668 1.0798722771622895 >10 1.2754667430874662 13.25478383277684 >50 0.12917049867224342 4.906463894009033 >100 0.06503065115373119 6.269242433647203 >500 0.0014847180628702768 0.4874770539399893 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.669399785103417E-5 0.0 0.0 0.0 0.0 7 1.5338799570206835E-4 0.0 0.0 0.0 0.0 8 1.5338799570206835E-4 0.0 0.0 0.0 0.0 9 1.5338799570206835E-4 0.0 0.0 0.0 0.0 10-11 1.5338799570206835E-4 0.0 0.0 0.0 0.0 12-13 1.5338799570206835E-4 0.0 0.0 0.0 7.669399785103417E-5 14-15 1.5338799570206835E-4 0.0 0.0 0.0 7.669399785103417E-5 16-17 1.5338799570206835E-4 0.0 0.0 0.0 7.669399785103417E-5 18-19 1.5338799570206835E-4 0.0 0.0 0.0 7.669399785103417E-5 20-21 1.5338799570206835E-4 0.0 0.0 7.669399785103417E-5 7.669399785103417E-5 22-23 2.3008199355310254E-4 0.0 0.0 4.21816988180688E-4 7.669399785103417E-5 24-25 2.3008199355310254E-4 0.0 0.0 8.43633976361376E-4 7.669399785103417E-5 26-27 2.3008199355310254E-4 0.0 0.0 0.0023775139333820595 7.669399785103417E-5 28-29 3.451229903296538E-4 0.0 0.0 0.007861134779731004 7.669399785103417E-5 30-31 3.834699892551709E-4 0.0 0.0 0.024580426311256454 7.669399785103417E-5 32-33 3.834699892551709E-4 0.0 0.0 0.05966793032810459 7.669399785103417E-5 34-35 3.834699892551709E-4 0.0 0.0 0.1074482909892989 7.669399785103417E-5 36-37 3.834699892551709E-4 0.0 0.0 0.17835189200257998 7.669399785103417E-5 38-39 3.834699892551709E-4 0.0 0.0 0.2992216326158098 7.669399785103417E-5 40-41 3.834699892551709E-4 0.0 0.0 0.4363121537745335 7.669399785103417E-5 42-43 4.21816988180688E-4 0.0 0.0 0.5640076601965054 7.669399785103417E-5 44-45 4.985109860317222E-4 0.0 0.0 0.709112704130662 7.669399785103417E-5 46-47 6.902459806593076E-4 0.0 0.0 0.8892669050827413 7.669399785103417E-5 48-49 7.669399785103418E-4 0.0 0.0 1.0842230476200703 7.669399785103417E-5 50-51 7.669399785103418E-4 0.0 0.0 1.3090898493193024 7.669399785103417E-5 52-53 7.669399785103418E-4 0.0 0.0 1.5177358704730408 7.669399785103417E-5 54-55 7.669399785103418E-4 0.0 0.0 1.7325174114548623 7.669399785103417E-5 56-57 7.669399785103418E-4 0.0 0.0 1.9856459513622005 7.669399785103417E-5 58-59 7.669399785103418E-4 0.0 0.0 2.2679565574518574 7.669399785103417E-5 60-61 7.669399785103418E-4 0.0 0.0 2.5830921946217567 7.669399785103417E-5 62-63 7.669399785103418E-4 0.0 0.0 2.893012639937786 7.669399785103417E-5 64-65 7.669399785103418E-4 0.0 0.0 3.1941899694987974 7.669399785103417E-5 66-67 8.43633976361376E-4 0.0 0.0 3.536283546913335 7.669399785103417E-5 68-69 8.43633976361376E-4 0.0 0.0 3.927116159962205 7.669399785103417E-5 70-71 8.43633976361376E-4 0.0 0.0 4.359095102858156 7.669399785103417E-5 72-73 8.43633976361376E-4 0.0 0.0 4.802002940447878 7.669399785103417E-5 74-75 8.43633976361376E-4 0.0 0.0 5.222477783666172 7.669399785103417E-5 76-77 8.43633976361376E-4 0.0 0.0 5.657601180474015 7.669399785103417E-5 78-79 8.43633976361376E-4 0.0 0.0 6.155805390514333 7.669399785103417E-5 80-81 9.203279742124102E-4 0.0 0.0 6.723571056605539 7.669399785103417E-5 82-83 9.203279742124102E-4 0.0 0.0 7.310203446168099 7.669399785103417E-5 84-85 9.203279742124102E-4 0.0 0.0 7.870951611455936 7.669399785103417E-5 86-87 9.203279742124102E-4 0.0 0.0 8.423186742982308 7.669399785103417E-5 88-89 9.203279742124102E-4 0.0 0.0 9.02964453098936 7.669399785103417E-5 90-91 9.203279742124102E-4 0.0 0.0 9.70412989509028 7.669399785103417E-5 92-93 9.970219720634443E-4 0.0 0.0 10.421372162993151 7.669399785103417E-5 94-95 9.970219720634443E-4 0.0 0.0 11.124732817284986 7.669399785103417E-5 96-97 9.970219720634443E-4 0.0 0.0 11.814787062949666 7.669399785103417E-5 98-99 9.970219720634443E-4 0.0 0.0 12.528578100949243 7.669399785103417E-5 100-101 0.0010737159699144784 0.0 0.0 13.323664776670913 7.669399785103417E-5 102-103 0.0011504099677655127 0.0 0.0 14.154414161393316 7.669399785103417E-5 104-105 0.0014571859591696495 0.0 0.0 14.97204887248319 7.669399785103417E-5 106-107 0.0014571859591696495 0.0 0.0 15.765294892256437 7.669399785103417E-5 108-109 0.0014571859591696495 0.0 0.0 16.556125051097375 7.669399785103417E-5 110-111 0.0014955329580951665 0.0 0.0 17.41766707595697 7.669399785103417E-5 112-113 0.0015338799570206835 0.0 0.0 18.3341220032779 7.669399785103417E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1515 0.0 71.11755 1 GTATCAA 3065 0.0 59.04104 1 TCAACGC 3975 0.0 44.018753 4 ATCAACG 4020 0.0 43.377964 3 CAACGCA 4095 0.0 42.728825 5 TATCAAC 4320 0.0 41.329967 2 AACGCAG 4230 0.0 41.221283 6 GTACATG 9595 0.0 39.084667 1 TACATGG 10025 0.0 36.74801 2 ACATGGG 9910 0.0 35.25267 3 CCGCAAT 70 0.0027898375 33.998222 9 ACGCAGA 5210 0.0 33.232746 7 CGCAGAG 5180 0.0 33.194214 8 CATGGGA 7780 0.0 32.89398 4 TTAGACG 80 0.0053557944 29.757574 3 ATGGGAT 3210 0.0 29.47947 5 CATGGGG 2435 0.0 28.596603 4 GCAGAGT 6075 0.0 27.128624 9 ATGGGAG 2875 0.0 27.118208 5 CGTATAG 155 6.8085883E-6 26.882967 1 >>END_MODULE