FastQCFastQC Report
Thu 26 May 2016
SRR1364407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303883
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74460.5710635079988006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65420.5017321339414657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.2986464276319271No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.11818545068844367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39300.081.970821
GTATCAA75050.059.1892281
TGGTATC13800.046.587122
GTGGTAT15350.044.611211
TATCAAC100600.044.0246732
ATCAACG101200.043.763663
TCAACGC101450.043.538464
CAACGCA103400.042.774955
AACGCAG106150.041.703676
GGACCGA3700.038.594986
CGCAGAG119550.036.87858
ACGCAGA120400.036.6675647
GTACATG94900.035.388931
TACATGG98400.033.8172232
ACATGGG98050.033.330823
GCAGAGT132950.032.7587559
CATGGGA77300.031.5737064
CAGAGTA128350.028.97268910-11
GAGTACT84300.028.4787312-13
AGGACCG4600.028.4699085
Produced by FastQC (version 0.11.5)