##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364407_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1303883 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.266950332200054 33.0 31.0 34.0 30.0 34.0 2 32.26412109061933 33.0 31.0 34.0 30.0 34.0 3 32.3623162507679 34.0 31.0 34.0 30.0 34.0 4 35.988265051388815 37.0 35.0 37.0 35.0 37.0 5 35.93896308181026 37.0 35.0 37.0 35.0 37.0 6 36.01109301984917 37.0 35.0 37.0 35.0 37.0 7 35.96389783439158 37.0 35.0 37.0 35.0 37.0 8 35.97465340065021 37.0 35.0 37.0 35.0 37.0 9 37.71308928791924 39.0 38.0 39.0 35.0 39.0 10-11 37.67256916456461 39.0 37.5 39.0 35.0 39.0 12-13 37.64453674141008 39.0 37.0 39.0 35.0 39.0 14-15 38.97505144249906 40.0 38.0 41.0 35.0 41.0 16-17 39.007891045438896 40.0 38.0 41.0 35.0 41.0 18-19 39.11571705436761 41.0 38.5 41.0 36.0 41.0 20-21 39.133320244224365 41.0 39.0 41.0 35.0 41.0 22-23 39.088038574013154 41.0 39.0 41.0 35.0 41.0 24-25 39.0345180510828 41.0 39.0 41.0 35.0 41.0 26-27 38.925646319493396 41.0 38.0 41.0 35.0 41.0 28-29 38.820379972742955 40.5 38.0 41.0 35.0 41.0 30-31 38.63340767538192 40.0 38.0 41.0 34.5 41.0 32-33 38.42649800633953 40.0 38.0 41.0 34.0 41.0 34-35 38.285641809886314 40.0 38.0 41.0 34.0 41.0 36-37 38.13699925530128 40.0 38.0 41.0 33.5 41.0 38-39 38.01735393436374 40.0 37.5 41.0 33.0 41.0 40-41 37.87048837970892 40.0 37.0 41.0 33.0 41.0 42-43 37.658483161449304 40.0 37.0 41.0 33.0 41.0 44-45 37.386869450709916 40.0 37.0 41.0 32.5 41.0 46-47 36.98891388261063 40.0 37.0 41.0 31.0 41.0 48-49 36.633421480301536 40.0 36.5 41.0 30.0 41.0 50-51 36.44809618654435 40.0 36.0 41.0 30.0 41.0 52-53 36.29999739240407 40.0 36.0 41.0 30.0 41.0 54-55 36.11603034934883 40.0 35.5 41.0 29.0 41.0 56-57 35.92361431202033 39.0 35.0 41.0 29.0 41.0 58-59 35.71053844555071 39.0 35.0 41.0 28.0 41.0 60-61 35.459535096323826 39.0 35.0 41.0 28.0 41.0 62-63 35.22009144992304 38.0 35.0 41.0 28.0 41.0 64-65 34.92552706032673 37.5 35.0 40.0 28.0 41.0 66-67 34.6040185354054 37.0 34.0 40.0 27.0 41.0 68-69 34.258984893583246 36.5 34.0 39.5 26.0 41.0 70-71 33.98790075489902 36.0 34.0 39.0 26.0 41.0 72-73 33.78455045429689 36.0 34.0 39.0 26.0 41.0 74-75 33.45022981356456 35.0 34.0 38.5 26.0 40.0 76-77 33.117584936685276 35.0 34.0 37.0 26.0 39.0 78-79 32.79366591941148 35.0 34.0 37.0 26.0 39.0 80-81 32.50005867090836 35.0 34.0 36.0 26.0 39.0 82-83 32.21668163477858 35.0 33.0 36.0 25.0 37.0 84-85 31.978384180175677 35.0 33.0 35.5 25.0 37.0 86-87 31.79230268359968 35.0 33.0 35.0 25.0 37.0 88-89 31.6218985905944 35.0 33.0 35.0 24.5 36.0 90-91 31.468274377378954 35.0 33.0 35.0 24.0 36.0 92-93 31.34051636534873 35.0 33.0 35.0 23.5 36.0 94-95 31.23935659871323 35.0 33.0 35.0 23.0 36.0 96-97 31.14910386898211 35.0 33.0 35.0 23.0 35.0 98-99 31.065187213883455 35.0 33.0 35.0 22.0 35.0 100-101 30.99976148166668 35.0 33.0 35.0 20.0 35.0 102-103 30.030085521477005 34.0 30.5 35.0 19.0 35.0 104-105 30.72699429319962 35.0 32.0 35.0 19.0 35.0 106-107 31.004037555516867 35.0 33.0 35.0 20.0 35.0 108-109 31.01518349422456 35.0 33.0 35.0 20.0 35.0 110-111 30.960471530037587 35.0 33.0 35.0 19.0 35.0 112-113 30.874055417548966 35.0 33.0 35.0 19.0 35.0 114-115 30.774406906141117 35.0 33.0 35.0 18.0 35.0 116-117 30.700880370401332 35.0 33.0 35.0 16.0 35.0 118-119 30.581352007810516 35.0 33.0 35.0 8.5 35.0 120-121 30.423318656658612 35.0 33.0 35.0 2.0 35.0 122-123 30.2520521396475 35.0 33.0 35.0 2.0 35.0 124-125 29.554878390162308 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06107605151051132 1101 2 0.1134836077817667 1101 3 0.12311319469158377 1101 4 0.05948585440178533 1101 5 0.19556206355737515 1101 6 0.2578128238557511 1101 7 0.21070529555918682 1101 8 0.18957528741796636 1101 9 0.16090823688469413 1101 10-11 0.24901486699270237 1101 12-13 0.17312235883984783 1101 14-15 0.2385667965492715 1101 16-17 0.17406249302251098 1101 18-19 0.17254308281988529 1101 20-21 0.19585204284484803 1101 22-23 0.2753241146365184 1101 24-25 0.2452866076959097 1101 26-27 0.09161093233905149 1101 28-29 0.01916591666334 1101 30-31 0.2020010182567873 1101 32-33 0.16195703333932698 1101 34-35 0.16676836206677592 1101 36-37 0.22358382248531683 1101 38-39 0.20902684770500457 1101 40-41 0.19708247978545046 1101 42-43 0.2384755760710746 1101 44-45 0.25370980010408317 1101 46-47 0.3109357911798156 1101 48-49 0.3451566261495671 1101 50-51 0.4025530191640243 1101 52-53 0.36170814434760246 1101 54-55 0.23203688813541135 1101 56-57 0.28745467076326037 1101 58-59 0.25807624197096857 1101 60-61 0.1974983955554208 1101 62-63 0.14908475539080257 1101 64-65 0.24516234884251986 1101 66-67 0.2052942040073944 1101 68-69 0.274241784222248 1101 70-71 0.1622671586468627 1101 72-73 0.19259107868280978 1101 74-75 0.15571469264709492 1101 76-77 0.058402387325315885 1101 78-79 0.014307952591025241 1101 80-81 0.014299338391396077 1101 82-83 -0.04893637874828016 1101 84-85 -0.15637537093049048 1101 86-87 -0.09056175993940485 1101 88-89 -0.2000620282051777 1101 90-91 -0.5919788014933545 1101 92-93 -0.35020059259298364 1101 94-95 -0.19462783235850623 1101 96-97 -0.11447325614851778 1101 98-99 -0.019372443163394593 1101 100-101 -0.14716119540766215 1101 102-103 0.02034675901306926 1101 104-105 -0.07000029082439596 1101 106-107 0.0227635455647075 1101 108-109 0.09949904383415031 1101 110-111 0.13624505443785395 1101 112-113 0.017060986011706802 1101 114-115 -0.03424223180110175 1101 116-117 0.03484135814714051 1101 118-119 -0.0461484543659374 1101 120-121 -0.01301248946552036 1101 122-123 -0.011473123235070659 1101 124-125 0.02543494795043344 1102 1 0.1452223366609502 1102 2 0.16009011115326643 1102 3 0.18572294014895618 1102 4 0.17589411418667567 1102 5 0.05435057092194029 1102 6 0.1247831018998582 1102 7 0.18494025200125463 1102 8 0.22563316375450881 1102 9 0.2153747934565402 1102 10-11 0.21076360047695886 1102 12-13 0.2989009819741 1102 14-15 0.40909908556154306 1102 16-17 0.2806287498107807 1102 18-19 0.25104184394935913 1102 20-21 0.267655951332479 1102 22-23 0.2404984253590996 1102 24-25 0.20361766257684621 1102 26-27 0.18779428153041522 1102 28-29 0.16912894497569653 1102 30-31 0.3068692204180934 1102 32-33 0.24872201978404718 1102 34-35 0.1940766773851479 1102 36-37 0.22243593816507 1102 38-39 0.18322033631335444 1102 40-41 0.19044868966798134 1102 42-43 0.033929638217543356 1102 44-45 0.06089758832831649 1102 46-47 0.14240808459101828 1102 48-49 0.1953752664420918 1102 50-51 0.1649927616928295 1102 52-53 0.24409807686895135 1102 54-55 0.2083566285255074 1102 56-57 0.293426950199958 1102 58-59 0.32727071487074966 1102 60-61 0.27587715819171876 1102 62-63 0.15049010483248537 1102 64-65 0.044963394378761734 1102 66-67 0.08319133218522978 1102 68-69 0.06142703880740896 1102 70-71 0.05083716546399586 1102 72-73 0.09312918838148221 1102 74-75 -0.031084379445573518 1102 76-77 -0.008206042097953059 1102 78-79 0.05373566003044772 1102 80-81 0.18854520102561878 1102 82-83 0.27872208913526464 1102 84-85 0.16899142952689772 1102 86-87 0.07974086107071798 1102 88-89 0.06170238103160486 1102 90-91 0.0891698605386324 1102 92-93 0.12117764022718447 1102 94-95 -0.02986944552710824 1102 96-97 -0.027912115245374736 1102 98-99 0.06392367544736643 1102 100-101 -0.008804147941727791 1102 102-103 0.12761371586343628 1102 104-105 0.11968193493803625 1102 106-107 0.03577882517031128 1102 108-109 0.023086129930483423 1102 110-111 0.06343103205190559 1102 112-113 -0.030149003452731193 1102 114-115 -0.05294878171379125 1102 116-117 -0.08074210186814668 1102 118-119 -0.02194749243371419 1102 120-121 -0.013815871782540512 1102 122-123 -0.05872731467611203 1102 124-125 -0.0615082134721483 1103 1 0.07741707763587868 1103 2 0.06901633404659435 1103 3 0.10847427922455921 1103 4 0.008391394898296767 1103 5 -0.007322828932622372 1103 6 -0.0030231492571246577 1103 7 0.05143045227880805 1103 8 0.12931046438136207 1103 9 0.2062556493034009 1103 10-11 0.14297739764406714 1103 12-13 0.11081802885717451 1103 14-15 0.1753169637785561 1103 16-17 0.16423746024498342 1103 18-19 0.18901454338300283 1103 20-21 0.1142670646617745 1103 22-23 0.1702917836409128 1103 24-25 0.21392373527107367 1103 26-27 0.2890832363194491 1103 28-29 0.28906120777449473 1103 30-31 0.15335631725863408 1103 32-33 0.2049523661953785 1103 34-35 0.16975704394159408 1103 36-37 0.252930836550199 1103 38-39 0.2509325829718634 1103 40-41 0.22559753365281665 1103 42-43 0.06625423353056448 1103 44-45 0.1245475387822097 1103 46-47 -0.02443039028445071 1103 48-49 -0.14137895586564042 1103 50-51 -0.29519219831337296 1103 52-53 -0.16651930745996424 1103 54-55 -0.23237301794885923 1103 56-57 -0.21018737424896017 1103 58-59 -0.10055471347010325 1103 60-61 -0.21579619326616495 1103 62-63 -0.16346156866416095 1103 64-65 -0.0854177113159551 1103 66-67 -0.2729341144253752 1103 68-69 -0.2617786845837955 1103 70-71 -0.16469290520725366 1103 72-73 -0.08220607960907955 1103 74-75 -0.11805926035950165 1103 76-77 -0.05316973248987722 1103 78-79 0.0741825005137855 1103 80-81 5.293983001593006E-4 1103 82-83 -0.0465773955611013 1103 84-85 -0.024591201147153186 1103 86-87 0.04289631846167907 1103 88-89 0.05761649144620762 1103 90-91 -0.021294499542335643 1103 92-93 -0.06177435752720939 1103 94-95 -0.11816328717075564 1103 96-97 -0.07421866222705731 1103 98-99 -0.08111890153057644 1103 100-101 -0.036602292655054214 1103 102-103 -0.056301980752305525 1103 104-105 -0.08276817716076934 1103 106-107 -0.0813842401785152 1103 108-109 -0.03310695103836636 1103 110-111 -0.0644189273553799 1103 112-113 -0.03782547286663274 1103 114-115 -0.031103579700008765 1103 116-117 -0.07882226612647258 1103 118-119 -0.1213616808879614 1103 120-121 -0.22477926211506016 1103 122-123 -0.2249345153759279 1103 124-125 -0.32551829571020363 1104 1 0.12408162877119366 1104 2 0.0698663743359802 1104 3 0.11184559222731849 1104 4 0.17117481051422345 1104 5 0.21381200146122836 1104 6 0.204102681072591 1104 7 0.2366680952557303 1104 8 0.2221575503956359 1104 9 0.2745969280160665 1104 10-11 0.29039917083090216 1104 12-13 0.3088639724845166 1104 14-15 0.318874624658136 1104 16-17 0.31401693990829216 1104 18-19 0.37574192747993607 1104 20-21 0.35920288311893955 1104 22-23 0.4169327241019687 1104 24-25 0.4011582027103344 1104 26-27 0.22278429493469787 1104 28-29 0.34032563920397507 1104 30-31 0.3495304917742885 1104 32-33 0.3580894349819772 1104 34-35 0.36474522320010294 1104 36-37 0.46132570561178454 1104 38-39 0.39821207593893604 1104 40-41 0.3424857756210571 1104 42-43 0.26376340542327625 1104 44-45 0.3218809609940081 1104 46-47 0.32690638559535756 1104 48-49 0.3435132934754037 1104 50-51 0.3786158337411365 1104 52-53 0.3994910123372435 1104 54-55 0.35869066393273386 1104 56-57 0.4513197205766062 1104 58-59 0.4692506791182751 1104 60-61 0.45621457073659855 1104 62-63 0.4146315066863764 1104 64-65 0.4250673325962637 1104 66-67 0.45315965776214995 1104 68-69 0.5167219619789734 1104 70-71 0.4758725298299211 1104 72-73 0.5739352584243562 1104 74-75 0.71263315977383 1104 76-77 0.6173280438215301 1104 78-79 0.5550073000681266 1104 80-81 0.5445002879912622 1104 82-83 0.5147973338474117 1104 84-85 0.5899480167989708 1104 86-87 0.5564966830127247 1104 88-89 0.46981643212543744 1104 90-91 0.5319579338555442 1104 92-93 0.5020786524416465 1104 94-95 0.45118165525756737 1104 96-97 0.4207913071910987 1104 98-99 0.42287068624609603 1104 100-101 0.299699693190302 1104 102-103 0.3423873607507524 1104 104-105 0.41674854622660007 1104 106-107 0.45108869927200956 1104 108-109 0.5141181785612687 1104 110-111 0.4430489636680548 1104 112-113 0.5195431440940084 1104 114-115 0.5021575025427225 1104 116-117 0.4238390461732884 1104 118-119 0.41117721720775435 1104 120-121 0.3740856050055754 1104 122-123 0.305648169737502 1104 124-125 0.15424584401681685 1105 1 0.018451270292572985 1105 2 0.0016228258217054758 1105 3 0.03444694379466284 1105 4 0.058406473675169934 1105 5 -0.020797190560642775 1105 6 0.06057590439660743 1105 7 0.05674335637333883 1105 8 0.07053884497636886 1105 9 0.08524457001908559 1105 10-11 0.08112533991961612 1105 12-13 0.07730363891889169 1105 14-15 0.07313849782988768 1105 16-17 0.16765508449744715 1105 18-19 0.1721534383924208 1105 20-21 0.08900955698884161 1105 22-23 0.03907846513041591 1105 24-25 0.02354889134475968 1105 26-27 0.13929019392304554 1105 28-29 0.11347844921520789 1105 30-31 0.09994100211776669 1105 32-33 0.05218543845999335 1105 34-35 0.07254803651460406 1105 36-37 -0.011591666994142713 1105 38-39 -0.00957387475080651 1105 40-41 -0.026933308288214164 1105 42-43 0.028982390447772843 1105 44-45 0.037141822868591134 1105 46-47 -0.06502763225931574 1105 48-49 -0.16659375888450967 1105 50-51 -0.19354288058629265 1105 52-53 -0.1538740003536816 1105 54-55 -0.2261018325455595 1105 56-57 -0.18608150120446965 1105 58-59 -0.24598687344089853 1105 60-61 -0.3483615939832774 1105 62-63 -0.2283592889311521 1105 64-65 -0.2277980882074715 1105 66-67 -0.169104494588602 1105 68-69 -0.2719252825669045 1105 70-71 -0.11908336095354599 1105 72-73 -0.10642476527388567 1105 74-75 -0.12080459929256193 1105 76-77 -0.1592593605170407 1105 78-79 -0.07928592419359859 1105 80-81 -0.040191645440529555 1105 82-83 -0.04924108096996349 1105 84-85 -0.12034143428396504 1105 86-87 -0.05293375985893434 1105 88-89 0.026410517579787296 1105 90-91 0.02219386142244062 1105 92-93 0.08160311044012758 1105 94-95 -0.029733076869117525 1105 96-97 -0.02462200559807215 1105 98-99 -0.1261707814256141 1105 100-101 -0.17268129876891436 1105 102-103 -0.04771667604973118 1105 104-105 -0.11562822608055257 1105 106-107 -0.21223088289718817 1105 108-109 -0.08146892848363052 1105 110-111 -0.05574654410740365 1105 112-113 -0.07495042306772248 1105 114-115 -0.055992046828272635 1105 116-117 -0.0599950778120224 1105 118-119 -0.21658526965910951 1105 120-121 -0.23692880574558828 1105 122-123 -0.18955836944452642 1105 124-125 -0.4101355507273752 1106 1 0.04125392369223846 1106 2 0.00846309234773912 1106 3 0.03260430513610402 1106 4 -0.04346788166604654 1106 5 -0.01723290259222665 1106 6 -0.051576191657815684 1106 7 0.013013781599276797 1106 8 0.03660146503910511 1106 9 0.10625250395519004 1106 10-11 0.0063839711991420245 1106 12-13 -0.03374022647106756 1106 14-15 -0.04390823953480094 1106 16-17 -0.053290925130873745 1106 18-19 1.604605400942205E-4 1106 20-21 -0.01997166504064296 1106 22-23 -0.10391466309817332 1106 24-25 -0.04151054963679712 1106 26-27 -0.02895767380515224 1106 28-29 -0.1250883220794492 1106 30-31 -0.09086396622909376 1106 32-33 -0.03787352597708349 1106 34-35 -0.09605591583942896 1106 36-37 0.07315845955488243 1106 38-39 -0.00801266208276985 1106 40-41 -0.2215432195662217 1106 42-43 -0.08490326852852803 1106 44-45 -0.03210750308571164 1106 46-47 0.05512061543367963 1106 48-49 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54-55 -0.8491409815820177 2216 56-57 -0.9597986655053319 2216 58-59 -0.986780867385896 2216 60-61 -1.1512597013417363 2216 62-63 -1.0230412200056378 2216 64-65 -1.0803633063869853 2216 66-67 -1.1139373475100527 2216 68-69 -1.0864516236412172 2216 70-71 -1.0338005071256617 2216 72-73 -1.002280986154588 2216 74-75 -1.0844972444585324 2216 76-77 -1.0651024833755827 2216 78-79 -0.9853479517130381 2216 80-81 -0.9470225585856866 2216 82-83 -0.985434451285947 2216 84-85 -1.007836222268228 2216 86-87 -0.9850964291361421 2216 88-89 -1.0161406054963003 2216 90-91 -0.997580526279016 2216 92-93 -1.019680405087147 2216 94-95 -0.9561210593204841 2216 96-97 -1.0959826589582349 2216 98-99 -1.107868448255438 2216 100-101 -1.2013383209403763 2216 102-103 -1.1154676685988143 2216 104-105 -1.1540176678963263 2216 106-107 -1.05940665019655 2216 108-109 -0.9028060144682968 2216 110-111 -0.9252573851001316 2216 112-113 -0.9412590469142792 2216 114-115 -0.8839313970938605 2216 116-117 -0.8244303219156492 2216 118-119 -0.8027679893342459 2216 120-121 -0.7335990075185777 2216 122-123 -0.7698188795875609 2216 124-125 -0.8463427442155265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 203.0 3 11.0 4 40.0 5 157.0 6 398.0 7 780.0 8 1271.0 9 2251.0 10 3925.0 11 5318.0 12 6853.0 13 10161.0 14 16326.0 15 14060.0 16 5910.0 17 4731.0 18 4744.0 19 4222.0 20 4108.0 21 3939.0 22 4040.0 23 4305.0 24 5306.0 25 6796.0 26 8899.0 27 11461.0 28 14651.0 29 18150.0 30 23356.0 31 29030.0 32 36743.0 33 47736.0 34 65066.0 35 99092.0 36 192333.0 37 425317.0 38 221129.0 39 1065.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56711517513309 19.41353810276316 13.430552785406343 22.588793936697403 2 15.800880907259318 21.41863955584972 39.24585258033121 23.534626956559755 3 18.28185504374242 27.645425241375182 30.54453505414213 23.528184660740266 4 11.9173269380765 17.452332762985638 38.14974196304423 32.48059833589363 5 11.80282556932283 39.970195776302575 34.43377730167415 13.793201352700452 6 29.79167002262105 38.411295030195916 17.55114282711734 14.245892120065692 7 26.84397296383188 34.78878089521836 21.517881589068956 16.849364551880804 8 26.922277535637782 34.271479879713134 20.196290618099937 18.609951966549147 9 27.90334715614821 14.718575209585522 20.285178961609283 37.09289867265698 10-11 24.35874231046804 27.421440420651237 27.54231016126447 20.67750710761625 12-13 25.926175891548553 25.38671031066438 28.012214286097752 20.674899511689315 14-15 22.803733157039396 25.57583770936503 27.18315216932808 24.4372769642675 16-17 20.714435267581525 29.300865184989753 28.067050494561247 21.917649052867475 18-19 20.877985217998855 26.509970603190624 31.70733110256058 20.90471307624994 20-21 21.807958480825874 25.7170620444604 32.03229286141856 20.44268661329517 22-23 21.665901004921455 26.005745914319 31.742725382568835 20.585627698190713 24-25 20.9758851062557 26.188162588207685 32.004712079227964 20.831240226308648 26-27 20.608438593013076 26.529082272619725 31.785898762806504 21.076580371560695 28-29 20.86159035401661 26.27727485553688 31.81873785115879 21.04239693928772 30-31 20.799605485318633 25.962156808081723 31.932374170792492 21.305863535807156 32-33 20.753028264827645 26.165442885169053 31.700874772410387 21.38065407759291 34-35 21.404185429569658 26.151322916478925 31.476849297155685 20.967642356795736 36-37 21.532108325670325 25.62810466890051 31.331223737099112 21.508563268330057 38-39 21.170035961815593 26.0947109518262 31.198121303828643 21.537131782529567 40-41 21.36794744610415 25.733301479698483 31.450965023251747 21.44778605094562 42-43 21.275681943855393 25.743989299653418 31.149995820177118 21.830332936314072 44-45 21.214427844861667 26.06157077924643 31.023946577967397 21.700054797924505 46-47 21.99089182081521 26.519097188934897 29.751404075365656 21.738606914884233 48-49 21.74518762122501 26.79827161611103 29.188872876812766 22.267667885851196 50-51 21.967155028480317 26.96491939844296 29.20154645777267 21.866379115304056 52-53 22.16360670397574 26.818548903544258 29.052223243956703 21.965621148523297 54-55 21.537323517524197 27.0495895720705 29.27758855664197 22.135498353763335 56-57 21.735002296985236 27.182692005340968 29.04781333908027 22.034492358593525 58-59 21.58641595389155 26.388497429791414 29.39087686198718 22.634209754329856 60-61 21.20631222279913 26.81528940863559 29.6766274274609 22.30177094110438 62-63 21.337612347120103 26.70205072080854 29.883777915656545 22.076559016414816 64-65 21.094338985936623 26.685216388280235 29.714590956397164 22.50585366938598 66-67 21.146222475482844 26.869128595127016 29.350984712585408 22.63366421680473 68-69 21.618158991259186 26.43515560828694 29.517909198908182 22.42877620154569 70-71 21.325826004893848 26.622396283711364 29.196249812578827 22.855527898815957 72-73 21.42733884457247 26.730391807227804 28.829356359974568 23.012912988225153 74-75 21.433058027445714 27.091234412903614 28.628028741842638 22.84767881780804 76-77 21.57669236046374 26.926680070236443 28.845911227362304 22.650716341937517 78-79 21.691056636216594 26.83480803108868 28.647317282302172 22.826818050392557 80-81 21.705489971258803 27.17778318898823 28.308832693255308 22.80789414649766 82-83 21.699032811993103 27.05472806992652 28.286740451405535 22.959498666674847 84-85 21.58457529723729 26.97455483910552 28.362103180309578 23.078766683347617 86-87 21.7901069344412 27.003496479362028 28.190182708111077 23.016213878085686 88-89 21.766983694089117 27.0015024354179 28.125222891931255 23.106290978561727 90-91 21.538950610561386 27.16909965779112 28.045981154736392 23.2459685769111 92-93 21.56412806977313 26.681458382385536 28.278419152638694 23.475994395202637 94-95 21.479764672136994 26.430208845425547 28.38605917862262 23.703967303814835 96-97 21.929766704527935 26.25354422137569 28.213267601464242 23.60342147263213 98-99 21.62906095652974 26.370409853667514 27.974928674417892 24.025600515384852 100-101 21.351263878737587 26.414870045855345 28.27489122873755 23.958974846669527 102-103 21.313108614806698 26.08769345102283 28.2621983720932 24.33699956207727 104-105 21.179513118079008 26.426361247319036 27.983765233994074 24.410360400607882 106-107 21.308238545943155 26.42959529344274 27.721122217254155 24.54104394335995 108-109 21.483356245937564 26.34527664812567 27.641919136722098 24.52944796921466 110-111 21.466090057089563 26.535353384283283 27.626716243739114 24.37184031488804 112-113 21.12214056015762 26.721300914269147 27.82899232523163 24.327566200341593 114-115 21.1484082544216 26.881246246787484 27.418794477725378 24.55155102106554 116-117 20.972978776845306 26.871286331398725 27.561455882719493 24.594279009036473 118-119 20.87073763520193 27.136100401646466 27.207847636636107 24.785314326515493 120-121 20.873509973137814 27.286919208284523 27.288338053229694 24.551232765347972 122-123 20.79705523806164 27.336680025569947 27.22953781211305 24.636726924255363 124-125 20.613084149421383 27.343634359831366 27.2327731859377 24.810508304809556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 322.0 1 253.5 2 379.0 3 577.0 4 377.5 5 276.0 6 684.5 7 1637.5 8 2900.0 9 3389.5 10 2508.5 11 1589.5 12 1429.0 13 1489.0 14 1632.0 15 1790.0 16 1929.0 17 2056.0 18 2286.0 19 2552.5 20 2842.0 21 3276.0 22 3838.0 23 4562.0 24 5335.0 25 6544.0 26 8112.5 27 9904.0 28 11948.5 29 14456.0 30 17552.0 31 20766.0 32 25270.0 33 28345.5 34 29558.5 35 30995.0 36 34853.5 37 39812.0 38 41058.0 39 41711.5 40 41733.0 41 41265.0 42 41341.0 43 42125.5 44 44562.0 45 47539.5 46 46963.5 47 45060.0 48 43230.0 49 40902.5 50 40798.0 51 40165.0 52 39864.5 53 40957.0 54 41971.0 55 39739.5 56 35406.5 57 32149.0 58 30343.0 59 27267.5 60 21960.5 61 17731.5 62 14244.5 63 10021.5 64 6923.0 65 5423.5 66 4018.5 67 3048.5 68 2296.5 69 1485.0 70 873.0 71 535.5 72 364.5 73 282.5 74 244.5 75 184.0 76 104.0 77 55.5 78 35.5 79 15.0 80 6.5 81 3.0 82 1.0 83 0.5 84 1.5 85 1.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023238281348863358 2 0.0 3 0.0 4 0.0 5 0.05460612646993634 6 0.01763961950573786 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.684289924786196E-4 22-23 0.0 24-25 0.0 26-27 3.451229903296538E-4 28-29 2.684289924786196E-4 30-31 1.9173499462758544E-4 32-33 7.669399785103417E-5 34-35 1.9173499462758544E-4 36-37 0.0 38-39 0.0 40-41 1.9173499462758544E-4 42-43 0.0 44-45 1.1504099677655127E-4 46-47 0.0 48-49 4.601639871062051E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.834699892551709E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 1.1504099677655127E-4 72-73 1.5338799570206835E-4 74-75 0.0 76-77 1.1504099677655127E-4 78-79 0.0 80-81 4.2181698818068804E-4 82-83 0.0 84-85 3.834699892551709E-5 86-87 0.0 88-89 0.0 90-91 7.669399785103417E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.3008199355310254E-4 100-101 0.0 102-103 0.0 104-105 0.0011120629688399955 106-107 0.0 108-109 0.0011887569666910298 110-111 0.004179822882881362 112-113 0.0 114-115 0.0 116-117 3.834699892551709E-5 118-119 0.0 120-121 4.2181698818068804E-4 122-123 6.518989817337905E-4 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1303883.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.83614576180474 #Duplication Level Percentage of deduplicated Percentage of total 1 77.52934994123571 38.6376398448939 2 11.911747308653547 11.87271150303687 3 4.094864737462543 6.122168277931727 4 1.9755350148946602 3.9381220383935753 5 1.1314516671210724 2.8193595102541367 6 0.6710681264995735 2.0066069381000378 7 0.45346146226149353 1.5819140071437439 8 0.31614715924184994 1.2604444728125863 9 0.2354770913199208 1.0561743581926841 >10 1.433648694238104 13.546729890161743 >50 0.16083473614073873 5.57000157908496 >100 0.08180121070315445 7.446642477177509 >500 0.003075233485080627 0.9782444366383506 >1k 9.22570045524188E-4 1.019470780591933 >5k 6.150466970161253E-4 2.1437698855862837 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7446 0.5710635079988006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6542 0.5017321339414657 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3894 0.2986464276319271 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1541 0.11818545068844367 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.669399785103417E-5 0.0 22-23 0.0 0.0 0.0 4.21816988180688E-4 0.0 24-25 0.0 0.0 0.0 8.43633976361376E-4 0.0 26-27 0.0 0.0 0.0 0.0024542079312330936 0.0 28-29 0.0 0.0 0.0 0.007899481778656521 0.0 30-31 0.0 0.0 0.0 0.024542079312330937 0.0 32-33 0.0 0.0 0.0 0.05962958332917907 0.0 34-35 0.0 0.0 0.0 0.10721820899574579 0.0 36-37 0.0 0.0 0.0 0.1777383400197717 0.0 38-39 0.0 0.0 0.0 0.2987231216297781 0.0 40-41 0.0 0.0 0.0 0.43596703078420385 0.0 42-43 3.834699892551709E-5 0.0 0.0 0.5633174142158461 0.0 44-45 1.5338799570206835E-4 0.0 0.0 0.7086525401435557 0.0 46-47 2.3008199355310254E-4 0.0 0.0 0.8888834350934862 0.0 48-49 2.3008199355310254E-4 0.0 0.0 1.0833410666447834 0.0 50-51 2.3008199355310254E-4 0.0 0.0 1.3083612563397176 0.0 52-53 2.3008199355310254E-4 0.0 0.0 1.517582482477339 0.0 54-55 2.3008199355310254E-4 0.0 0.0 1.733782862419404 0.0 56-57 2.3008199355310254E-4 0.0 0.0 1.9883685882859123 0.0 58-59 2.3008199355310254E-4 0.0 0.0 2.272289768330441 0.0 60-61 2.3008199355310254E-4 0.0 0.0 2.588230692477776 0.0 62-63 2.3008199355310254E-4 0.0 0.0 2.899723364749751 0.0 64-65 2.3008199355310254E-4 0.0 0.0 3.2021661452753047 0.0 66-67 3.067759914041367E-4 0.0 0.0 3.5458702966447144 0.0 68-69 3.067759914041367E-4 0.0 0.0 3.9389653826301902 0.0 70-71 3.067759914041367E-4 0.0 0.0 4.373360186458447 0.0 72-73 3.067759914041367E-4 0.0 0.0 4.818223720993371 0.0 74-75 3.067759914041367E-4 0.0 0.0 5.240769302153645 0.0 76-77 3.067759914041367E-4 0.0 0.0 5.677158149926029 0.0 78-79 3.067759914041367E-4 0.0 0.0 6.179158712859973 0.0 80-81 3.067759914041367E-4 0.0 0.0 6.750950813838358 0.0 82-83 3.067759914041367E-4 0.0 0.0 7.341226168298842 0.0 84-85 3.834699892551709E-4 0.0 0.0 7.906154156469561 0.0 86-87 4.601639871062051E-4 0.0 0.0 8.46184051789923 0.0 88-89 4.601639871062051E-4 0.0 0.0 9.073475150761226 0.0 90-91 4.601639871062051E-4 0.0 0.0 9.75152678576222 0.0 92-93 5.368579849572392E-4 0.0 0.0 10.474291021510366 0.0 94-95 5.368579849572392E-4 0.0 0.0 11.181524722693677 0.0 96-97 5.368579849572392E-4 0.0 0.0 11.875183586257355 0.0 98-99 5.368579849572392E-4 0.0 0.0 12.594688327096833 0.0 100-101 6.135519828082734E-4 0.0 0.0 13.394031519699237 0.0 102-103 6.902459806593076E-4 0.0 0.0 14.229114115300224 0.0 104-105 8.43633976361376E-4 0.0 0.0 15.051733936250415 0.0 106-107 8.43633976361376E-4 0.0 0.0 15.849658289892576 0.0 108-109 8.43633976361376E-4 0.0 0.0 16.64539686459598 0.0 110-111 8.43633976361376E-4 0.0 0.0 17.513457879272913 0.0 112-113 8.43633976361376E-4 0.0 0.0 18.43524303944449 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3930 0.0 81.97082 1 GTATCAA 7505 0.0 59.189228 1 TGGTATC 1380 0.0 46.58712 2 GTGGTAT 1535 0.0 44.61121 1 TATCAAC 10060 0.0 44.024673 2 ATCAACG 10120 0.0 43.76366 3 TCAACGC 10145 0.0 43.53846 4 CAACGCA 10340 0.0 42.77495 5 AACGCAG 10615 0.0 41.70367 6 GGACCGA 370 0.0 38.59498 6 CGCAGAG 11955 0.0 36.8785 8 ACGCAGA 12040 0.0 36.667564 7 GTACATG 9490 0.0 35.38893 1 TACATGG 9840 0.0 33.817223 2 ACATGGG 9805 0.0 33.33082 3 GCAGAGT 13295 0.0 32.758755 9 CATGGGA 7730 0.0 31.573706 4 CAGAGTA 12835 0.0 28.972689 10-11 GAGTACT 8430 0.0 28.47873 12-13 AGGACCG 460 0.0 28.469908 5 >>END_MODULE