FastQCFastQC Report
Thu 26 May 2016
SRR1364406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470885
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30450.047.5126271
TCAACGC30550.045.198724
ATCAACG30800.044.6386033
CAACGCA31600.044.261915
AACGCAG34850.040.1301236
TATCAAC36000.038.686792
GGTATCA19500.036.6384161
GTACATG84750.035.8980831
ACATGGG85000.034.2404373
TACATGG89150.033.6479452
ACGCAGA42450.032.795977
CGCAGAG43500.031.7288748
CATGGGG52100.027.3029484
GAGTACA63650.026.3779321
CATGGGA41500.026.245354
AGTACAT67950.023.5622
TAAGGTG13450.021.683195
GGACCGA7750.021.501156
GCAGAGT62300.021.1992689
AGAGTAC49050.020.07312210-11
Produced by FastQC (version 0.11.5)