FastQCFastQC Report
Thu 26 May 2016
SRR1364406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470885
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37580.1520912547528517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34280.13873571615028624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33450.065.353031
GTATCAA56250.052.100071
ATCAACG68250.042.143043
TCAACGC69300.041.590444
CAACGCA69400.041.4447065
AACGCAG72600.039.6750566
TATCAAC73050.039.2923662
GTACATG85800.033.8093951
ACGCAGA87150.033.1107377
CGCAGAG87900.032.8282248
TACATGG89500.032.137012
ACATGGG86200.032.054733
CATGGGG53650.027.6382794
GCAGAGT108550.026.528349
CAGAGTA99700.024.64616610-11
TATTCCG4400.024.3613415
AGAGTAC97850.024.32168610-11
GAGTACT65300.024.23611512-13
GAGTACA65750.022.7391221
CATGGGA38450.022.4570664
Produced by FastQC (version 0.11.5)