##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364406_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2470885 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.303444717176234 33.0 31.0 34.0 30.0 34.0 2 32.3274199325343 34.0 31.0 34.0 30.0 34.0 3 32.43916450988209 34.0 31.0 34.0 30.0 34.0 4 36.032440198552344 37.0 35.0 37.0 35.0 37.0 5 35.96708466804404 37.0 35.0 37.0 35.0 37.0 6 36.02017576698228 37.0 35.0 37.0 35.0 37.0 7 35.972106350558605 37.0 35.0 37.0 35.0 37.0 8 35.9845355004381 37.0 35.0 37.0 35.0 37.0 9 37.747844193477235 39.0 38.0 39.0 35.0 39.0 10-11 37.700495773781455 39.0 37.5 39.0 35.0 39.0 12-13 37.66619166816748 39.0 37.0 39.0 35.0 39.0 14-15 39.06560523860884 41.0 38.0 41.0 36.0 41.0 16-17 39.08109219975839 41.0 38.0 41.0 36.0 41.0 18-19 39.13190253694526 41.0 38.5 41.0 36.0 41.0 20-21 39.13337447918458 41.0 39.0 41.0 35.5 41.0 22-23 39.09136463251021 41.0 39.0 41.0 35.0 41.0 24-25 39.05965473909146 41.0 39.0 41.0 35.0 41.0 26-27 38.9675444223426 41.0 39.0 41.0 35.0 41.0 28-29 38.88348547180463 40.5 38.0 41.0 35.0 41.0 30-31 38.78555477086145 40.0 38.0 41.0 35.0 41.0 32-33 38.676832390014106 40.0 38.0 41.0 34.5 41.0 34-35 38.585979922173635 40.0 38.0 41.0 34.0 41.0 36-37 38.47414408197872 40.0 38.0 41.0 34.0 41.0 38-39 38.38864779218782 40.0 38.0 41.0 34.0 41.0 40-41 38.28826594519777 40.0 38.0 41.0 34.0 41.0 42-43 38.15222339364236 40.0 38.0 41.0 33.0 41.0 44-45 38.007356675846914 40.0 38.0 41.0 33.0 41.0 46-47 37.81072733049089 40.0 37.5 41.0 33.0 41.0 48-49 37.62055498333593 40.0 37.0 41.0 33.0 41.0 50-51 37.468687737389644 40.0 37.0 41.0 32.0 41.0 52-53 37.3201073704361 40.0 37.0 41.0 32.0 41.0 54-55 37.143542293550695 40.0 36.0 41.0 31.5 41.0 56-57 36.94635140850343 39.5 36.0 41.0 31.0 41.0 58-59 36.72871056321925 39.0 35.5 41.0 31.0 41.0 60-61 36.48337235443981 39.0 35.0 41.0 31.0 41.0 62-63 36.24072366783561 38.5 35.0 41.0 31.0 41.0 64-65 35.94933009832509 38.0 35.0 40.0 30.0 41.0 66-67 35.62597551079876 37.0 35.0 40.0 30.0 41.0 68-69 35.28949404767927 37.0 35.0 39.5 29.5 41.0 70-71 35.03497532260708 36.0 34.5 39.0 29.5 41.0 72-73 34.83666156053398 36.0 34.5 39.0 30.0 41.0 74-75 34.50200859206316 35.5 34.0 38.5 29.5 40.0 76-77 34.165146900806796 35.0 34.0 37.0 29.0 39.5 78-79 33.852120798823094 35.0 34.0 37.0 29.0 39.0 80-81 33.56123190678643 35.0 34.0 36.5 29.0 39.0 82-83 33.28133462301969 35.0 34.0 36.0 29.0 37.0 84-85 33.04451623608546 35.0 34.0 36.0 29.0 37.0 86-87 32.85700993773486 35.0 34.0 35.0 29.0 37.0 88-89 32.697836605103035 35.0 34.0 35.0 29.0 36.0 90-91 32.556189583894025 35.0 34.0 35.0 29.0 36.0 92-93 32.4319952162889 35.0 33.0 35.0 27.5 36.0 94-95 32.34905428621728 35.0 33.0 35.0 27.5 36.0 96-97 32.26933790929161 35.0 33.0 35.0 27.0 35.0 98-99 32.20378669990712 35.0 33.0 35.0 27.0 35.0 100-101 32.142612869477944 35.0 33.0 35.0 27.0 35.0 102-103 31.155836269191 34.0 31.5 35.0 25.0 35.0 104-105 31.889317592684403 35.0 33.0 35.0 26.0 35.0 106-107 32.190696855580086 35.0 33.5 35.0 28.0 35.0 108-109 32.20643979788618 35.0 34.0 35.0 28.0 35.0 110-111 32.17611948755203 35.0 34.0 35.0 27.0 35.0 112-113 32.1120541830154 35.0 34.0 35.0 27.0 35.0 114-115 32.039728882566365 35.0 34.0 35.0 27.0 35.0 116-117 31.985755306297136 35.0 34.0 35.0 27.0 35.0 118-119 31.878042078040863 35.0 33.0 35.0 27.0 35.0 120-121 31.748717362402537 35.0 33.0 35.0 26.0 35.0 122-123 31.602815995078686 35.0 33.0 35.0 25.0 35.0 124-125 30.91296256199702 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09525293110920785 1101 2 0.12154714861875959 1101 3 0.14420109854733454 1101 4 0.14235641744149063 1101 5 0.19378053609862178 1101 6 0.1981270469852987 1101 7 0.23826955442379472 1101 8 0.20703586217627645 1101 9 0.2028268267318012 1101 10-11 0.23793603775206407 1101 12-13 0.2039348239623635 1101 14-15 0.23173465402535953 1101 16-17 0.23873044279025635 1101 18-19 0.26514698371220646 1101 20-21 0.2459202217121117 1101 22-23 0.22093960961133519 1101 24-25 0.19076145773411213 1101 26-27 0.17014468387196047 1101 28-29 0.14119787177591547 1101 30-31 0.14219973962750743 1101 32-33 0.1656803143415999 1101 34-35 0.14931407250808348 1101 36-37 0.22961738868151116 1101 38-39 0.20543580730468847 1101 40-41 0.17045783269794157 1101 42-43 0.13195049703331563 1101 44-45 0.04476530561282743 1101 46-47 0.11031504499735689 1101 48-49 -0.006228322110942486 1101 50-51 -0.10095976164541298 1101 52-53 -0.06579467113167681 1101 54-55 -0.035232597443055624 1101 56-57 -0.014308960890424771 1101 58-59 -0.17852252310497363 1101 60-61 -0.12435607055257947 1101 62-63 -0.29167110142710584 1101 64-65 -0.4073693659095028 1101 66-67 -0.2599104643071115 1101 68-69 -0.26450523866072473 1101 70-71 -0.2221999668885246 1101 72-73 -0.2118467792452563 1101 74-75 -0.20289646647920279 1101 76-77 -0.2655700021210379 1101 78-79 -0.440401535597168 1101 80-81 -0.4308237457571735 1101 82-83 -0.35060144510016755 1101 84-85 -0.48651646317125596 1101 86-87 -0.44542765287953046 1101 88-89 -0.5212672152054694 1101 90-91 -0.8661779478095575 1101 92-93 -0.7729876267449356 1101 94-95 -0.5425292529722157 1101 96-97 -0.41234972191725916 1101 98-99 -0.3062008825720426 1101 100-101 -0.42657765530281466 1101 102-103 -0.282389703963684 1101 104-105 -0.3759636810131184 1101 106-107 -0.20935580441148716 1101 108-109 -0.20086370798507858 1101 110-111 -0.2542202358013803 1101 112-113 -0.27118380314631807 1101 114-115 -0.3914132084802233 1101 116-117 -0.3320288508495217 1101 118-119 -0.29322881826006864 1101 120-121 -0.3937467618187718 1101 122-123 -0.3525317982585925 1101 124-125 -0.43847647912613397 1102 1 0.05019061646633105 1102 2 0.09614879322763414 1102 3 0.02574815228456373 1102 4 0.09783597953012446 1102 5 0.07457899512436228 1102 6 0.07052968478547683 1102 7 0.19665994191959868 1102 8 0.22818307215292322 1102 9 0.28166827679739725 1102 10-11 0.25679470250353376 1102 12-13 0.21109746415653063 1102 14-15 0.31227886962315665 1102 16-17 0.34759372227453866 1102 18-19 0.30165948207815063 1102 20-21 0.2342388446002417 1102 22-23 0.3173763428845078 1102 24-25 0.29700583697379557 1102 26-27 0.25664989789003556 1102 28-29 0.3290458864170631 1102 30-31 0.3367477005613537 1102 32-33 0.3219129992498537 1102 34-35 0.25369984054609773 1102 36-37 0.3014043612750399 1102 38-39 0.33427977736089787 1102 40-41 0.3019047561831485 1102 42-43 0.37302886401639057 1102 44-45 0.3609447478444068 1102 46-47 0.26802739751884275 1102 48-49 0.2793657971759558 1102 50-51 0.2858395431521785 1102 52-53 0.21756957146151734 1102 54-55 0.19672533288865424 1102 56-57 0.22295693591910748 1102 58-59 0.27360060836136313 1102 60-61 0.22527412082495601 1102 62-63 0.1737154493829962 1102 64-65 0.16746919719184916 1102 66-67 0.06563019180394747 1102 68-69 0.020085373114241634 1102 70-71 0.03805741865271273 1102 72-73 0.1310638571626228 1102 74-75 0.15752723844249772 1102 76-77 0.13329155274466586 1102 78-79 0.12022459544549946 1102 80-81 0.10104929239076199 1102 82-83 0.08464633297685964 1102 84-85 0.0863879283848874 1102 86-87 0.0743102917216234 1102 88-89 0.0525235436739564 1102 90-91 0.07804249309430134 1102 92-93 0.1219492396152333 1102 94-95 -0.012204197808046047 1102 96-97 0.0085391102718404 1102 98-99 0.09546758746291317 1102 100-101 0.03391708185428399 1102 102-103 0.09335337826833623 1102 104-105 0.015515521976848134 1102 106-107 0.007690475216115544 1102 108-109 0.09293254762386738 1102 110-111 0.05339005710028033 1102 112-113 0.0022496289391469304 1102 114-115 -0.08240518703510347 1102 116-117 -0.05883233725653625 1102 118-119 -0.10173453470127569 1102 120-121 -0.07669319784329431 1102 122-123 -0.2051695590579321 1102 124-125 -0.245703300331229 1103 1 0.06871456362954831 1103 2 0.12743928413128458 1103 3 0.1778121990004351 1103 4 0.11585424843956105 1103 5 0.004434825521343555 1103 6 0.08282133068757247 1103 7 0.11928384042045082 1103 8 0.16099821963508276 1103 9 0.2206570961779235 1103 10-11 0.2555048565228688 1103 12-13 0.2513516517587675 1103 14-15 0.3437342556095615 1103 16-17 0.31490785253726017 1103 18-19 0.2982670405755954 1103 20-21 0.2323414518480078 1103 22-23 0.2858161569121691 1103 24-25 0.266245941803092 1103 26-27 0.2831518355904734 1103 28-29 0.3208047966144676 1103 30-31 0.3363380278890773 1103 32-33 0.31080493360841643 1103 34-35 0.2435562279220207 1103 36-37 0.23579810434944903 1103 38-39 0.28942538338330337 1103 40-41 0.280901519842331 1103 42-43 0.3066017960121954 1103 44-45 0.2884085002408767 1103 46-47 0.26404060085133807 1103 48-49 0.15839363439739884 1103 50-51 0.0881684480143079 1103 52-53 0.1887334522563009 1103 54-55 0.20878172847268672 1103 56-57 0.16450419411695094 1103 58-59 0.1137278633932226 1103 60-61 0.11809524850211517 1103 62-63 0.1137427413513592 1103 64-65 0.20355776857016394 1103 66-67 0.03580396923515394 1103 68-69 -0.0010512450268578277 1103 70-71 0.04623914622551695 1103 72-73 0.20817466079530078 1103 74-75 0.2288879673227413 1103 76-77 0.1188460106282676 1103 78-79 0.1891866505219113 1103 80-81 0.23344831797075472 1103 82-83 0.19066214531599712 1103 84-85 0.10518230348382218 1103 86-87 0.12567914076860376 1103 88-89 0.10680993638514735 1103 90-91 0.21521541453681436 1103 92-93 0.18426638929013706 1103 94-95 0.11921132021169001 1103 96-97 0.14262739436627925 1103 98-99 0.17717200166762836 1103 100-101 0.08314229482544988 1103 102-103 0.15943380016522823 1103 104-105 0.16327763920040184 1103 106-107 0.09314057283400956 1103 108-109 0.18291689358401442 1103 110-111 0.1646524645391736 1103 112-113 0.13657902628793295 1103 114-115 0.18564532802384548 1103 116-117 0.10252592919046677 1103 118-119 0.0424138151753084 1103 120-121 0.09618134118333899 1103 122-123 0.10270568352105514 1103 124-125 -0.01548118299042045 1104 1 0.05278802588772891 1104 2 0.04208490687754818 1104 3 0.043606095455274385 1104 4 0.030540790370658044 1104 5 0.10927829556861468 1104 6 0.11335997243075724 1104 7 0.11916435335839992 1104 8 0.09418776285148311 1104 9 0.10608010813871971 1104 10-11 0.15677473550780263 1104 12-13 0.16469656922712517 1104 14-15 0.15412376593850752 1104 16-17 0.15977337330956232 1104 18-19 0.17646097119567372 1104 20-21 0.10310942508655074 1104 22-23 0.15535529964915895 1104 24-25 0.13487398618474344 1104 26-27 0.07629607716212661 1104 28-29 0.1795562579731822 1104 30-31 0.1486036219964788 1104 32-33 0.12586596213874657 1104 34-35 0.1636568419607798 1104 36-37 0.17584765763439947 1104 38-39 0.1308905807419336 1104 40-41 0.054701178752779356 1104 42-43 0.06419662108914537 1104 44-45 0.08880943219432424 1104 46-47 0.027205492920906238 1104 48-49 0.036296871477603077 1104 50-51 0.0075382971312834 1104 52-53 0.027969694910716214 1104 54-55 0.026929949777333206 1104 56-57 -0.0017350901715147415 1104 58-59 -0.018728070333509095 1104 60-61 0.031182481587151756 1104 62-63 0.07417020080161763 1104 64-65 -0.018936191857029883 1104 66-67 -0.08413765323486189 1104 68-69 -0.10835545642478905 1104 70-71 -0.005512822363762382 1104 72-73 0.06154741699978672 1104 74-75 -0.002031466224920564 1104 76-77 0.03374931775785228 1104 78-79 0.06269704578222957 1104 80-81 0.0963366100412486 1104 82-83 0.07013282295518763 1104 84-85 0.024567706454512006 1104 86-87 0.038981884095711905 1104 88-89 0.06935446927401756 1104 90-91 0.07807313130562932 1104 92-93 0.07116306233983494 1104 94-95 0.15296740399318765 1104 96-97 0.03483759089473892 1104 98-99 0.0036360205081038544 1104 100-101 -0.01695888508456278 1104 102-103 6.953523242216875E-4 1104 104-105 0.05750439856752365 1104 106-107 0.04186065800067951 1104 108-109 0.10917242233252011 1104 110-111 0.13347942331507312 1104 112-113 0.038591763410991575 1104 114-115 -0.03987950425502618 1104 116-117 -0.049823003149661815 1104 118-119 -0.12580670302737573 1104 120-121 -0.08762991884842108 1104 122-123 -0.15531632111285276 1104 124-125 -0.26559677208179266 1105 1 0.0836848257381746 1105 2 0.09659965634133272 1105 3 0.0726456846506025 1105 4 0.11357792125110677 1105 5 0.1076381660886625 1105 6 0.07097162846297778 1105 7 0.06022479312613882 1105 8 0.1310768825245603 1105 9 0.10244745572589409 1105 10-11 0.1272244984843809 1105 12-13 0.19990889461542594 1105 14-15 0.16454898122545103 1105 16-17 0.13883014602947696 1105 18-19 0.13287612770262314 1105 20-21 0.14871296039638793 1105 22-23 0.10439689441640354 1105 24-25 0.15463394021828236 1105 26-27 0.08609387750077957 1105 28-29 0.10380106227957242 1105 30-31 0.13008335678441085 1105 32-33 0.06773855924657823 1105 34-35 0.13673668414752171 1105 36-37 0.029230067435435103 1105 38-39 0.1351443009076334 1105 40-41 0.08334965305720488 1105 42-43 0.04240723110356015 1105 44-45 0.18712263132549367 1105 46-47 0.1577797134073009 1105 48-49 0.11470480480524259 1105 50-51 0.11912334004683345 1105 52-53 0.13520850462790435 1105 54-55 0.048893302560273355 1105 56-57 0.05598825522456252 1105 58-59 -0.002685977151855923 1105 60-61 -0.04633422040159729 1105 62-63 -0.014291016345083563 1105 64-65 -0.061509561890154885 1105 66-67 -0.03053916929972189 1105 68-69 -0.014702729854022323 1105 70-71 0.0029371386169430025 1105 72-73 0.06806343363326306 1105 74-75 0.0778238758725962 1105 76-77 0.04092115215264869 1105 78-79 0.12225419876651955 1105 80-81 0.08263698656113405 1105 82-83 0.10324968737540274 1105 84-85 0.09544173157493674 1105 86-87 0.007890271442853702 1105 88-89 0.031052158951624165 1105 90-91 0.05717273872981821 1105 92-93 0.0026499102417503195 1105 94-95 -0.05683144593755429 1105 96-97 -0.022094837217423446 1105 98-99 -0.029380494397948098 1105 100-101 0.02855635683647506 1105 102-103 0.09805881244922432 1105 104-105 0.09340895830761298 1105 106-107 0.005632674967756657 1105 108-109 0.05063039510496026 1105 110-111 0.040988588097910394 1105 112-113 -0.02115626640537016 1105 114-115 -0.0038197750895037075 1105 116-117 0.0057474019276391175 1105 118-119 -0.02033230725910329 1105 120-121 -0.04614585520229397 1105 122-123 -0.1011736804129022 1105 124-125 -0.20990183432118314 1106 1 0.10429982437252505 1106 2 0.12207103880322734 1106 3 0.1121498817756077 1106 4 0.06432416255250928 1106 5 0.110773021725592 1106 6 0.06279138952574925 1106 7 0.055874980251466866 1106 8 0.029659544954547812 1106 9 0.1399045604937541 1106 10-11 0.11520463500497158 1106 12-13 0.10694905951833533 1106 14-15 0.17911820161054948 1106 16-17 0.17296728867715672 1106 18-19 0.17023177358472452 1106 20-21 0.12515289797627105 1106 22-23 0.03789305806473209 1106 24-25 0.12036498714720523 1106 26-27 0.15965318888399338 1106 28-29 0.07544614593681587 1106 30-31 0.06455717756688983 1106 32-33 0.23881911808419432 1106 34-35 0.16129769273634764 1106 36-37 0.2514433553041613 1106 38-39 0.24884967036680905 1106 40-41 0.13262153310516567 1106 42-43 0.1610933877982461 1106 44-45 0.1665591372867965 1106 46-47 0.1577278987505295 1106 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2216 42-43 -0.7270887477087697 2216 44-45 -0.672358038483317 2216 46-47 -0.7414722255341175 2216 48-49 -0.7370429172447643 2216 50-51 -0.6127901501305715 2216 52-53 -0.6374901425792032 2216 54-55 -0.5845069997934615 2216 56-57 -0.6627609603154312 2216 58-59 -0.5104103643956819 2216 60-61 -0.7535610132415229 2216 62-63 -0.5302248155978972 2216 64-65 -0.5114361547125768 2216 66-67 -0.44967081746877113 2216 68-69 -0.6570176966075465 2216 70-71 -0.6691406366776462 2216 72-73 -0.6074313580397472 2216 74-75 -0.738062813129801 2216 76-77 -0.6919386252715256 2216 78-79 -0.5887028156280678 2216 80-81 -0.5612966791354594 2216 82-83 -0.6753757912518168 2216 84-85 -0.5686296164484475 2216 86-87 -0.5974160048952015 2216 88-89 -0.4869265066608932 2216 90-91 -0.4715485811078395 2216 92-93 -0.44976523342501906 2216 94-95 -0.4726152320662784 2216 96-97 -0.5836707128697611 2216 98-99 -0.6281056235124538 2216 100-101 -0.5979522435260236 2216 102-103 -0.5528685807356553 2216 104-105 -0.5445212273720905 2216 106-107 -0.5897764579379867 2216 108-109 -0.4351126307866622 2216 110-111 -0.41867383880869724 2216 112-113 -0.33608646256484676 2216 114-115 -0.331429891339468 2216 116-117 -0.35049806343657863 2216 118-119 -0.38671403165025 2216 120-121 -0.3217880400553135 2216 122-123 -0.2722658997638838 2216 124-125 -0.2861616740390964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 380.0 3 17.0 4 53.0 5 155.0 6 313.0 7 829.0 8 1362.0 9 2398.0 10 3951.0 11 5398.0 12 6930.0 13 9157.0 14 13343.0 15 11617.0 16 6169.0 17 5547.0 18 5657.0 19 5422.0 20 5361.0 21 5428.0 22 5918.0 23 6757.0 24 8701.0 25 11898.0 26 15884.0 27 20839.0 28 27157.0 29 34687.0 30 43914.0 31 55310.0 32 70351.0 33 92529.0 34 125931.0 35 192065.0 36 386129.0 37 844518.0 38 436739.0 39 2071.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.873231773446776 19.01302768479164 13.17115489792126 24.94258564384032 2 15.923201605902337 20.50313956335483 38.55966586870696 25.013992962035868 3 18.33739732929699 24.910588716188734 30.175665803952832 26.57634815056144 4 12.609368707973054 16.666740864103346 37.54929913775833 33.17459129016527 5 12.798514367766057 38.44652464992199 35.28919170964734 13.465769272664618 6 32.29925168055134 37.2434141047712 17.095215523853973 13.362118690823483 7 27.985033702499308 32.63911513486059 21.793689305653643 17.582161856986463 8 26.249623110747766 35.15562237821671 20.05038680472786 18.54436770630766 9 26.53417702564061 15.166023509795073 20.02331148556084 38.27648797900348 10-11 24.64141390635339 26.15884591957942 28.449239847261204 20.75050032680598 12-13 25.596557508746866 23.582522051815445 28.34765681122351 22.473263628214184 14-15 22.55762611372039 25.077755541030843 26.020798216023817 26.343820129224955 16-17 22.334892154025784 28.32547852287743 26.790117710860684 22.5495116122361 18-19 22.31941187064554 26.98504786746449 28.48374165531783 22.21179860657214 20-21 22.802818104149896 26.337367117234706 28.75205240559946 22.10776237301594 22-23 22.681002960477723 26.17123014628362 28.544752183934097 22.60301470930456 24-25 22.573819213322192 26.417058344896333 28.52996568637668 22.479156755404794 26-27 22.351247563869567 26.77247396722336 28.405660260907524 22.470618207999554 28-29 22.38024962366639 26.709538041009285 28.476540438333796 22.433671896990525 30-31 22.491375038169593 26.38255876801812 28.54638255472088 22.579683639091407 32-33 22.384735008645695 26.378793811522804 28.58762001025949 22.648851169572005 34-35 22.989528231119134 26.417232068798118 28.171423842057987 22.421815858024758 36-37 22.946798414333326 26.309095728858285 27.981451989874074 22.76265386693432 38-39 22.51197445449707 26.527661141655724 28.19184219419358 22.76852220965363 40-41 22.862308158195237 26.176770373835918 28.233342446133136 22.727579021835712 42-43 22.560438871092746 26.14381082081926 28.163431321166303 23.13231898692169 44-45 22.619104712587507 26.45906877401091 28.06110772163387 22.860718791767717 46-47 23.06009790014509 26.711178383453703 27.409268339076892 22.819455377324317 48-49 23.052310192822304 26.560478955641642 27.462911938923686 22.92429891261237 50-51 22.86980171072308 26.852382850679007 27.44654243317678 22.831273005421135 52-53 22.90329173555224 26.62904182104792 27.35952907561461 23.10813736778523 54-55 22.802983546381157 26.590229816442285 27.402084678161877 23.204701959014685 56-57 22.87314813091034 26.882141680582077 27.3854437403324 22.859266448175187 58-59 22.866071994086326 26.466444087711942 27.45180013545762 23.215683782744108 60-61 22.759279116445953 26.62410970646342 27.55314948958561 23.063461687505022 62-63 22.54860100733138 26.663057973155368 27.8208617560105 22.967479263502753 64-65 22.48937121719546 26.47557454110572 27.71604101364491 23.319013228053915 66-67 22.47296009324594 26.6558945479049 27.486811405629968 23.38433395321919 68-69 22.44100590760469 26.411125091718223 27.92347917728197 23.22438982339512 70-71 22.52423070533273 26.30765473159702 27.664294977889487 23.503819585180764 72-73 22.44639107395117 26.579189078549128 27.2765113488896 23.69790849861011 74-75 22.397343947076443 26.750319571797064 27.215659007938253 23.636677473188243 76-77 22.486021197271906 26.542545097220266 27.427147531005097 23.54428617450273 78-79 22.533323080596627 26.494737715433942 27.423878488881513 23.548060715087914 80-81 22.53016287944784 26.736807768417403 27.147572936162533 23.585456415972228 82-83 22.533464730248472 26.67014045574764 27.1522551636357 23.64413965036819 84-85 22.533383787590278 26.495122193060382 27.217110468516342 23.754383550833 86-87 22.462356605022087 26.383967687690845 27.34097297122286 23.812702736064203 88-89 22.395214670047373 26.567667050469773 27.27429645653278 23.762821822950077 90-91 22.4763642331319 26.564394248294487 27.160617989226125 23.798623529347488 92-93 22.252472292316316 26.087110488752007 27.3735523911473 24.286864827784377 94-95 22.164406680197583 26.14595580126149 27.435372346345538 24.25426517219539 96-97 22.26718362044369 26.09322165944591 27.374624881368415 24.26496983874199 98-99 22.056606530221405 26.08383406566322 27.276890307546175 24.582669096569198 100-101 22.016585150664643 25.986377350625382 27.388951731869348 24.608085766840627 102-103 21.872025989073954 25.750920368799108 27.472651999719126 24.90440164240781 104-105 21.660170952623783 25.976137862930315 27.387164792825168 24.97652639162073 106-107 21.765318903955468 25.896227465058065 27.277088978240588 25.061364652745876 108-109 21.742117437747623 25.918980496383938 27.235835286608918 25.10306677925952 110-111 21.58350920527468 26.02841324283846 27.28742632147806 25.100651230408804 112-113 21.4645966930877 26.070355358505147 27.57892415065857 24.88612379774858 114-115 21.4291640444618 26.234324948348466 27.13469060680687 25.201820400382857 116-117 21.2673639743177 26.006840870141843 27.537203782694007 25.188591372846442 118-119 21.06955604975545 26.17968865406524 27.18795087590074 25.56280442027856 120-121 21.008241815502238 26.311706113885542 27.33233625085521 25.347715819757006 122-123 20.864679417508693 26.35839327006141 27.361400511034677 25.415526801395217 124-125 20.79162761181828 26.331365616516194 27.370406704644978 25.506600067020546 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 76.0 1 70.5 2 110.0 3 164.0 4 128.0 5 110.5 6 224.5 7 556.5 8 1002.5 9 1206.5 10 970.0 11 697.0 12 689.5 13 795.5 14 895.5 15 1021.5 16 1218.5 17 1407.0 18 1592.5 19 1739.0 20 2041.5 21 2577.0 22 3188.0 23 4141.5 24 5713.5 25 7788.5 26 10897.5 27 14786.0 28 18154.0 29 22328.0 30 26968.5 31 31650.5 32 38239.0 33 43272.5 34 46861.0 35 52285.0 36 60074.0 37 67067.0 38 71594.0 39 76218.0 40 77627.5 41 78477.5 42 79775.5 43 80974.5 44 84455.5 45 88319.5 46 88416.5 47 87953.5 48 87919.0 49 86874.0 50 87565.5 51 85336.5 52 84704.5 53 88003.0 54 92371.5 55 87851.0 56 76655.0 57 69671.0 58 65050.5 59 58433.0 60 48200.5 61 39452.5 62 32169.0 63 24611.5 64 19092.0 65 15015.5 66 10442.5 67 7970.0 68 5754.5 69 3331.0 70 2288.0 71 1553.0 72 1097.5 73 875.0 74 730.5 75 565.0 76 345.5 77 182.5 78 110.0 79 70.0 80 46.0 81 30.0 82 17.5 83 5.0 84 5.0 85 3.0 86 1.5 87 2.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027682389103499357 2 0.0 3 0.0 4 0.0 5 0.055202892890603976 6 0.01683607290505224 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.6188531639473307E-4 22-23 0.0 24-25 2.0235664549341634E-5 26-27 3.84477626437491E-4 28-29 2.22592310042758E-4 30-31 1.821209809440747E-4 32-33 1.0117832274670817E-4 34-35 1.4164965184539142E-4 36-37 0.0 38-39 0.0 40-41 6.07069936480249E-5 42-43 0.0 44-45 1.0117832274670817E-4 46-47 0.0 48-49 3.642419618881494E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.214139872960498E-4 58-59 6.07069936480249E-5 60-61 2.0235664549341634E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.094265819736654E-5 70-71 6.07069936480249E-5 72-73 1.821209809440747E-4 74-75 2.0235664549341634E-5 76-77 4.047132909868327E-5 78-79 0.0 80-81 3.4400629733880776E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.0235664549341634E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.6188531639473307E-4 100-101 0.0 102-103 2.0235664549341634E-5 104-105 0.0010117832274670817 106-107 0.0 108-109 0.0014367321830032558 110-111 0.004067368574417668 112-113 0.0 114-115 0.0 116-117 2.0235664549341634E-5 118-119 0.0 120-121 3.84477626437491E-4 122-123 6.273056010295907E-4 124-125 4.047132909868327E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2470885.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.36583885091044 #Duplication Level Percentage of deduplicated Percentage of total 1 78.19738801513165 43.29463983407894 2 11.884137419304869 13.15950474478616 3 4.14235263897308 6.880344860190814 4 1.923406849353953 4.2596413466427325 5 1.0618836560100475 2.9396039688533953 6 0.6174756833739873 2.0512235508023986 7 0.41014766588986074 1.589571870231456 8 0.29265283538468706 1.2962375778536481 9 0.21018702760791375 1.0473462989182452 >10 1.096012735769465 11.02658908302938 >50 0.0981178562158855 3.823107628075759 >100 0.06258974827158002 6.504835656867422 >500 0.002879896352411457 1.0375804872410301 >1k 7.679723606430551E-4 1.0897730924285463 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3758 0.1520912547528517 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3428 0.13873571615028624 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 4.047132909868327E-5 0.0 0.0 0.0 8 0.0 4.047132909868327E-5 0.0 0.0 0.0 9 0.0 4.047132909868327E-5 0.0 0.0 0.0 10-11 0.0 4.047132909868327E-5 0.0 2.0235664549341634E-5 0.0 12-13 0.0 4.047132909868327E-5 0.0 4.047132909868327E-5 0.0 14-15 0.0 4.047132909868327E-5 0.0 4.047132909868327E-5 0.0 16-17 0.0 4.047132909868327E-5 0.0 4.047132909868327E-5 0.0 18-19 0.0 4.047132909868327E-5 0.0 4.047132909868327E-5 0.0 20-21 0.0 4.047132909868327E-5 0.0 4.047132909868327E-5 0.0 22-23 0.0 4.047132909868327E-5 0.0 8.094265819736654E-5 0.0 24-25 0.0 4.047132909868327E-5 0.0 2.428279745920996E-4 0.0 26-27 0.0 4.047132909868327E-5 0.0 7.284839237762988E-4 0.0 28-29 0.0 4.047132909868327E-5 0.0 0.0037638336061775433 0.0 30-31 0.0 4.047132909868327E-5 0.0 0.012809175659733253 0.0 32-33 0.0 4.047132909868327E-5 0.0 0.03284248356358147 0.0 34-35 0.0 4.047132909868327E-5 0.0 0.06222466848922552 0.0 36-37 0.0 4.047132909868327E-5 0.0 0.10947494521193823 0.0 38-39 0.0 4.047132909868327E-5 0.0 0.1830315858487951 0.0 40-41 0.0 4.047132909868327E-5 0.0 0.26336717410968136 0.0 42-43 0.0 4.047132909868327E-5 0.0 0.3418613168965775 0.0 44-45 0.0 4.047132909868327E-5 0.0 0.43751530322131543 0.0 46-47 0.0 4.047132909868327E-5 0.0 0.5597184814347895 0.0 48-49 0.0 4.047132909868327E-5 0.0 0.6863330345200201 0.0 50-51 0.0 4.047132909868327E-5 0.0 0.8205561974758032 0.0 52-53 0.0 4.047132909868327E-5 0.0 0.9529986219512442 0.0 54-55 0.0 4.047132909868327E-5 0.0 1.0992822409784349 0.0 56-57 0.0 4.047132909868327E-5 0.0 1.2681893329717895 0.0 58-59 0.0 4.047132909868327E-5 0.0 1.4549038097685647 0.0 60-61 0.0 6.07069936480249E-5 0.0 1.6607409895644678 0.0 62-63 0.0 8.094265819736654E-5 0.0 1.861903730849473 0.0 64-65 0.0 8.094265819736654E-5 0.0 2.0706548463404815 0.0 66-67 0.0 1.0117832274670816E-4 0.0 2.3112164265030546 0.0 68-69 0.0 1.2141398729604979E-4 0.0 2.584053891621828 0.0 70-71 0.0 1.2141398729604979E-4 0.0 2.877976919201015 0.0 72-73 0.0 1.2141398729604979E-4 0.0 3.1823213140231132 2.0235664549341634E-5 74-75 0.0 1.2141398729604979E-4 0.0 3.485228976662208 4.047132909868327E-5 76-77 0.0 1.2141398729604979E-4 0.0 3.8142811178990526 4.047132909868327E-5 78-79 0.0 1.2141398729604979E-4 0.0 4.181861964437843 4.047132909868327E-5 80-81 0.0 1.2141398729604979E-4 0.0 4.5804033777371265 4.047132909868327E-5 82-83 0.0 1.2141398729604979E-4 0.0 4.9999696465031755 4.047132909868327E-5 84-85 0.0 1.2141398729604979E-4 0.0 5.419313322959182 4.047132909868327E-5 86-87 0.0 1.2141398729604979E-4 0.0 5.852862435928827 6.07069936480249E-5 88-89 0.0 1.2141398729604979E-4 0.0 6.316967402367977 8.094265819736654E-5 90-91 0.0 1.2141398729604979E-4 0.0 6.819560602779976 8.094265819736654E-5 92-93 0.0 1.2141398729604979E-4 0.0 7.343198894323289 8.094265819736654E-5 94-95 0.0 1.2141398729604979E-4 0.0 7.8775216167486555 8.094265819736654E-5 96-97 0.0 1.2141398729604979E-4 0.0 8.408889932149817 8.094265819736654E-5 98-99 0.0 1.2141398729604979E-4 0.0 8.982530550794554 8.094265819736654E-5 100-101 0.0 1.2141398729604979E-4 0.0 9.612972679829292 8.094265819736654E-5 102-103 0.0 1.2141398729604979E-4 0.0 10.260433002750027 8.094265819736654E-5 104-105 0.0 1.2141398729604979E-4 0.0 10.910503726397627 8.094265819736654E-5 106-107 0.0 1.2141398729604979E-4 0.0 11.557883106660164 8.094265819736654E-5 108-109 0.0 1.2141398729604979E-4 0.0 12.221107012264836 8.094265819736654E-5 110-111 0.0 1.2141398729604979E-4 0.0 12.931560958927673 8.094265819736654E-5 112-113 0.0 1.2141398729604979E-4 0.0 13.671053084218812 8.094265819736654E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3345 0.0 65.35303 1 GTATCAA 5625 0.0 52.10007 1 ATCAACG 6825 0.0 42.14304 3 TCAACGC 6930 0.0 41.59044 4 CAACGCA 6940 0.0 41.444706 5 AACGCAG 7260 0.0 39.675056 6 TATCAAC 7305 0.0 39.292366 2 GTACATG 8580 0.0 33.809395 1 ACGCAGA 8715 0.0 33.110737 7 CGCAGAG 8790 0.0 32.828224 8 TACATGG 8950 0.0 32.13701 2 ACATGGG 8620 0.0 32.05473 3 CATGGGG 5365 0.0 27.638279 4 GCAGAGT 10855 0.0 26.52834 9 CAGAGTA 9970 0.0 24.646166 10-11 TATTCCG 440 0.0 24.361341 5 AGAGTAC 9785 0.0 24.321686 10-11 GAGTACT 6530 0.0 24.236115 12-13 GAGTACA 6575 0.0 22.739122 1 CATGGGA 3845 0.0 22.457066 4 >>END_MODULE