FastQCFastQC Report
Thu 26 May 2016
SRR1364405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345970
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24800.050.175771
GGTATCA17800.042.479871
ATCAACG29000.042.2767873
CAACGCA30450.040.0681535
TCAACGC30400.039.9382744
TATCAAC33250.037.2309762
AACGCAG32900.037.081986
GTACATG78650.033.9896771
TACATGG80500.032.6782762
ACATGGG79500.032.1161083
ACGCAGA40550.029.930557
CGCAGAG41400.029.1717038
TAAGGTG12800.028.363035
CATGGGG59750.027.0934144
GTAAGGT12850.024.5473984
GAGTACA60600.024.1691821
GTGTAAG9150.024.0757641
TCTATAC4600.023.2888163
CATGGGA30250.022.6258644
GTATAGG6650.022.3829751
Produced by FastQC (version 0.11.5)