FastQCFastQC Report
Thu 26 May 2016
SRR1364405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345970
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29910.12749523651197586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27410.11683866375102835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.070.294981
GTATCAA47800.054.820931
ATCAACG62100.041.226653
TATCAAC62650.041.1498262
CAACGCA63500.040.411485
TCAACGC63450.040.349494
AACGCAG66550.038.5380446
GTACATG79100.033.655241
ACGCAGA78000.033.0285077
TACATGG80050.032.874732
CGCAGAG80650.031.721948
ACATGGG80650.031.3013233
CATGGGG60900.027.0809364
TAAGGTA9400.025.9691184
GCAGAGT98200.025.6891659
AGAGTAC84450.024.72880410-11
TAAGGTG14550.024.5521455
GAGTACT55300.024.36905712-13
CAGAGTA88700.024.24824510-11
GAGTACA61400.024.1519741
Produced by FastQC (version 0.11.5)