FastQCFastQC Report
Thu 26 May 2016
SRR1364404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909777
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.044.804891
GTATCAA24500.043.017031
TCAACGC24250.041.7242284
CAACGCA24950.040.792165
ATCAACG24950.040.792163
AACGCAG26750.038.044286
GTACATG67950.036.365691
TATCAAC29000.035.3005642
TACATGG69500.035.025932
ACATGGG69650.034.4377753
ACGCAGA32350.031.2655857
CATGGGG43750.031.1536254
CGCAGAG35450.028.697838
CATGGGA33250.023.6283514
GAGTACA46700.022.695321
ATGGGGA25500.022.4069215
TAAGGTG9600.022.3193955
TATACCG1400.003150764621.2565675
GTAAGGT10400.021.174814
TCTATAC5650.021.0684553
Produced by FastQC (version 0.11.5)