FastQCFastQC Report
Thu 26 May 2016
SRR1364404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909777
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22490.11776244032680255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.10917505028073958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.064.114841
GTATCAA43700.051.1029131
TCAACGC52700.041.800794
ATCAACG53550.041.0261043
CAACGCA54550.040.2740255
AACGCAG56350.038.9661036
TATCAAC57250.038.7906232
ACGCAGA66700.032.8261267
CGCAGAG69100.031.6859998
GTACATG70150.031.1554641
TACATGG71850.029.9139542
ACATGGG70650.029.6636033
GCAGAGT81850.026.4594239
CATGGGG43600.025.67234
AGAGTAC74100.024.24858510-11
CAGAGTA76950.023.81440710-11
CATGGGA33350.023.7437444
GAGTACT46050.023.57929812-13
TGGTATC14950.023.0983092
GAGTACA52350.022.9789681
Produced by FastQC (version 0.11.5)