FastQCFastQC Report
Thu 26 May 2016
SRR1364403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498652
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17000.047.278851
GGTATCA11650.042.416441
ATCAACG18750.040.315173
CAACGCA19450.038.8642355
TCAACGC19450.038.558224
AACGCAG20950.036.0755776
GTACATG51550.035.2251781
ACATGGG50100.034.8093573
GTACCGT1051.4821941E-534.0059976
TACATGG53200.033.787912
TATCAAC22650.033.3734862
ACGCAGA24900.030.1057057
TGGACCG2701.8189894E-1228.6579845
CATGGGG33950.028.5768174
CGCAGAG26550.028.2337888
CATGGGA23450.026.397124
ATGGGGA20550.023.1709695
GGACCGC1903.3019947E-521.9249216
GAGTACA37750.021.7642751
GTGTAAG9150.021.4721181
Produced by FastQC (version 0.11.5)