FastQCFastQC Report
Thu 26 May 2016
SRR1364403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498652
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.19584266394066133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.1951753976239981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.069.981881
GTATCAA38700.048.7860951
ATCAACG49150.038.030883
TCAACGC49750.037.452564
CAACGCA50850.036.6423765
TATCAAC52800.035.2891042
AACGCAG53500.034.8145946
GTACATG49150.032.8392681
ACATGGG47900.031.6909243
TACATGG50850.031.2572352
ACGCAGA62250.029.820417
GTGGTAT12800.029.3142191
CGCAGAG63950.029.0276838
CATGGGG35600.026.0858424
GCAGAGT72100.025.8289899
TGGTATC12750.025.6792742
GGACCGA2558.8220986E-1025.6698476
CAGAGTA68800.024.7761910-11
AGAGTAC66350.024.65983210-11
GAGTACT48200.024.5025812-13
Produced by FastQC (version 0.11.5)