FastQCFastQC Report
Thu 26 May 2016
SRR1364402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294792
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.062.9026071
GTATCAA26550.053.5907551
ATCAACG31700.043.370233
TCAACGC31550.043.1991464
GTACATG85150.041.529651
CAACGCA33050.041.238525
TATCAAC35600.039.454912
AACGCAG34650.038.9862376
TACATGG91450.037.7470782
ACATGGG90950.036.384063
ACGCAGA41500.032.5448767
CGCAGAG41550.032.3612638
CATGGGA71850.032.1398624
CATGGGG22000.030.8404874
CGACGGC800.005358122329.7549156
ATGGGAT30750.027.4841485
AGTACAT58900.027.3837572
GAGTACA63500.027.0007131
GCAGAGT49050.026.685289
ATGGGAG24000.025.7905835
Produced by FastQC (version 0.11.5)