##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364402_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1294792 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99876119098666 33.0 31.0 34.0 27.0 34.0 2 30.952243294675903 33.0 31.0 34.0 27.0 34.0 3 31.107961742117652 33.0 31.0 34.0 27.0 34.0 4 34.40387645274299 37.0 35.0 37.0 30.0 37.0 5 34.54750492743236 37.0 35.0 37.0 31.0 37.0 6 34.5610885763891 37.0 35.0 37.0 31.0 37.0 7 34.47295164010899 37.0 35.0 37.0 31.0 37.0 8 34.3633517970454 37.0 35.0 37.0 31.0 37.0 9 35.8251603346329 39.0 37.0 39.0 30.0 39.0 10-11 35.79383677069367 39.0 37.0 39.0 30.0 39.0 12-13 35.82231431766647 39.0 37.0 39.0 30.0 39.0 14-15 37.12947253304006 40.0 38.0 41.0 31.0 41.0 16-17 37.163516224999846 40.0 38.0 41.0 31.0 41.0 18-19 37.18084101539089 40.0 38.0 41.0 31.0 41.0 20-21 37.22925574146272 40.0 38.0 41.0 30.5 41.0 22-23 37.216597723804284 40.0 38.0 41.0 30.0 41.0 24-25 37.26759780721537 40.0 38.0 41.0 30.0 41.0 26-27 37.263998387385776 40.0 38.0 41.0 30.0 41.0 28-29 37.28861662722662 40.0 38.0 41.0 30.0 41.0 30-31 37.21308441819227 40.0 37.5 41.0 30.0 41.0 32-33 37.174315642975856 40.0 37.0 41.0 30.0 41.0 34-35 37.16276243597427 40.0 37.0 41.0 30.0 41.0 36-37 37.099426780517646 40.0 37.0 41.0 30.0 41.0 38-39 37.01137634461752 40.0 37.0 41.0 30.0 41.0 40-41 36.949075218259 40.0 37.0 41.0 30.0 41.0 42-43 36.82113420533955 40.0 37.0 41.0 30.0 41.0 44-45 36.71037664736885 40.0 37.0 41.0 30.0 41.0 46-47 36.61129857150801 40.0 36.0 41.0 30.0 41.0 48-49 36.483262562635545 40.0 36.0 41.0 29.0 41.0 50-51 36.379951760591666 40.0 36.0 41.0 29.0 41.0 52-53 36.238892810582705 40.0 35.5 41.0 29.0 41.0 54-55 36.065563040241216 39.5 35.0 41.0 29.0 41.0 56-57 35.88967030998029 39.0 35.0 41.0 29.0 41.0 58-59 35.687566419934626 39.0 35.0 41.0 28.0 41.0 60-61 35.449961074829005 39.0 35.0 41.0 28.0 41.0 62-63 35.18194505372291 38.0 34.5 40.0 28.0 41.0 64-65 34.8554088224209 37.5 34.0 40.0 28.0 41.0 66-67 34.54349231382338 37.0 34.0 40.0 27.5 41.0 68-69 34.21472444995027 36.5 34.0 39.5 26.5 41.0 70-71 33.861054516864485 36.0 34.0 39.0 26.0 41.0 72-73 33.485443221768435 36.0 33.0 39.0 26.0 40.5 74-75 33.121383975186745 35.0 33.0 37.5 26.0 40.0 76-77 32.878116716816294 35.0 33.0 37.0 26.0 39.0 78-79 32.694990778441635 35.0 33.0 37.0 26.0 39.0 80-81 32.417015628765085 35.0 33.0 36.5 26.0 39.0 82-83 32.11861017059111 35.0 33.0 36.0 25.5 37.5 84-85 31.85663566039951 35.0 33.0 36.0 25.0 37.0 86-87 31.646018820011243 35.0 33.0 35.0 25.0 37.0 88-89 31.38604424494436 35.0 32.5 35.0 24.0 36.0 90-91 31.26352611075756 35.0 32.0 35.0 24.0 36.0 92-93 31.114446181317156 35.0 32.0 35.0 23.0 36.0 94-95 31.017427509592274 35.0 32.0 35.0 23.0 35.5 96-97 30.92347380892066 35.0 32.0 35.0 22.5 35.0 98-99 30.804618039036384 35.0 32.0 35.0 20.0 35.0 100-101 30.731142144838707 35.0 32.0 35.0 20.0 35.0 102-103 29.745274144418563 33.5 30.0 34.5 19.0 35.0 104-105 30.44815808253372 34.5 31.5 35.0 19.0 35.0 106-107 30.779356452619417 35.0 32.0 35.0 20.0 35.0 108-109 30.814487191765167 35.0 33.0 35.0 20.0 35.0 110-111 30.763389795426605 35.0 33.0 35.0 19.0 35.0 112-113 30.68474086957596 35.0 32.5 35.0 19.0 35.0 114-115 30.60215579027365 35.0 32.0 35.0 18.0 35.0 116-117 30.482672892634493 35.0 32.0 35.0 12.5 35.0 118-119 30.328672095595277 35.0 32.0 35.0 4.5 35.0 120-121 30.169823415652864 35.0 32.0 35.0 2.0 35.0 122-123 30.004489524186123 35.0 32.0 35.0 2.0 35.0 124-125 29.216995471087248 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40394002565918896 1101 2 0.30069302727062563 1101 3 0.3603933891424731 1101 4 0.4876065410006376 1101 5 0.28449830593559966 1101 6 0.37769680127014027 1101 7 0.4285252999761724 1101 8 0.3869690976400406 1101 9 0.5759410452631712 1101 10-11 0.5797351910695951 1101 12-13 0.5944924711225852 1101 14-15 0.6869663591452024 1101 16-17 0.611841942531818 1101 18-19 0.4075712130393683 1101 20-21 0.5063781481437459 1101 22-23 0.66184899257469 1101 24-25 0.7308437289915233 1101 26-27 0.7247108933848878 1101 28-29 0.6732192796249876 1101 30-31 0.6875485140697464 1101 32-33 0.5588224540922511 1101 34-35 0.5787514092558439 1101 36-37 0.5031431820039032 1101 38-39 0.5883705684685978 1101 40-41 0.6285924413808104 1101 42-43 0.6339765915490148 1101 44-45 0.6596998385921964 1101 46-47 0.5934420711511379 1101 48-49 0.6016056030639874 1101 50-51 0.46607818904932685 1101 52-53 0.5658741223255888 1101 54-55 0.7545560641098916 1101 56-57 0.5744760400681344 1101 58-59 0.612409633139464 1101 60-61 0.6942863154476839 1101 62-63 0.6679729392991192 1101 64-65 0.6436403294326496 1101 66-67 0.5067188244854037 1101 68-69 0.5839998441866356 1101 70-71 0.55077976679123 1101 72-73 0.4895528008689638 1101 74-75 0.5323555627490748 1101 76-77 0.5748412455128289 1101 78-79 0.5898608380408135 1101 80-81 0.6358950663782608 1101 82-83 0.603278622409313 1101 84-85 0.5932531544844295 1101 86-87 0.551860285119016 1101 88-89 0.6851596653091079 1101 90-91 0.5909380587109077 1101 92-93 0.5587403402351434 1101 94-95 0.5452398507014564 1101 96-97 0.6470394996387832 1101 98-99 0.5594979627904486 1101 100-101 0.4949696563654058 1101 102-103 0.3322883516263637 1101 104-105 0.47895096324381825 1101 106-107 0.5425445817590955 1101 108-109 0.5702657517616494 1101 110-111 0.5581162929906078 1101 112-113 0.6485019889310557 1101 114-115 0.5698583518627132 1101 116-117 0.5208236535880566 1101 118-119 0.566506594946329 1101 120-121 0.39578795905124053 1101 122-123 0.31128717291539587 1101 124-125 0.18057349365451358 1102 1 0.44208953631018133 1102 2 0.3928804246029074 1102 3 0.46043836026964513 1102 4 0.5212896592491063 1102 5 0.4206746337145333 1102 6 0.46046500881658403 1102 7 0.4329788922476254 1102 8 0.469691224714623 1102 9 0.7254175625693762 1102 10-11 0.5798247919871145 1102 12-13 0.5824620545977908 1102 14-15 0.6256955450653976 1102 16-17 0.5248799853747173 1102 18-19 0.6219230613356288 1102 20-21 0.6216885555889888 1102 22-23 0.559267888810858 1102 24-25 0.5669209837559421 1102 26-27 0.5744045009140919 1102 28-29 0.5813723657792593 1102 30-31 0.7218609720959819 1102 32-33 0.7242449480185869 1102 34-35 0.6277998048447486 1102 36-37 0.5537408201237213 1102 38-39 0.6873642221636089 1102 40-41 0.5125810134237909 1102 42-43 0.49333984476206183 1102 44-45 0.5185374330891648 1102 46-47 0.5848583032533554 1102 48-49 0.575502295766114 1102 50-51 0.5655514209885553 1102 52-53 0.5428488198798291 1102 54-55 0.6037504665272451 1102 56-57 0.6560401819516102 1102 58-59 0.6352821873544201 1102 60-61 0.5741092981683522 1102 62-63 0.7042988579431935 1102 64-65 0.6629467671305704 1102 66-67 0.5632915637902514 1102 68-69 0.6025493674670912 1102 70-71 0.5985349224706908 1102 72-73 0.7233595683836 1102 74-75 0.5818596430041865 1102 76-77 0.5880369006363395 1102 78-79 0.7598452986816966 1102 80-81 0.6114323092129581 1102 82-83 0.6598820820287372 1102 84-85 0.6561209982511471 1102 86-87 0.47317019933253235 1102 88-89 0.5698279243120794 1102 90-91 0.46002130479001835 1102 92-93 0.4087424309232226 1102 94-95 0.38247466398594554 1102 96-97 0.42440041981460297 1102 98-99 0.4312820365260315 1102 100-101 0.3535311947022244 1102 102-103 0.01142405264261015 1102 104-105 0.35082250454173547 1102 106-107 0.3324372569267524 1102 108-109 0.34853974795403886 1102 110-111 0.41975967621885246 1102 112-113 0.3416811404188991 1102 114-115 0.30565212639096373 1102 116-117 0.22901821216700213 1102 118-119 0.12414430515648078 1102 120-121 0.10559320079024559 1102 122-123 0.13267073054969458 1102 124-125 0.1869116918909981 1103 1 0.5411297766260326 1103 2 0.5634209254474385 1103 3 0.5180405522075944 1103 4 0.42228876051257913 1103 5 0.44431630011892054 1103 6 0.4239123103339466 1103 7 0.34707873942744527 1103 8 0.4882919234210661 1103 9 0.4719887253665789 1103 10-11 0.6943201531777774 1103 12-13 0.5635457489993954 1103 14-15 0.6008106847542365 1103 16-17 0.5505022817339551 1103 18-19 0.7118133245570206 1103 20-21 0.6813137789617301 1103 22-23 0.7175596074958293 1103 24-25 0.641440929062405 1103 26-27 0.586456575177003 1103 28-29 0.6303368954113324 1103 30-31 0.7170215207099346 1103 32-33 0.662460746198029 1103 34-35 0.6979060620792197 1103 36-37 0.6138464478929251 1103 38-39 0.5355774150562524 1103 40-41 0.5682873783667688 1103 42-43 0.5691956552510504 1103 44-45 0.5675854542951484 1103 46-47 0.4551460563071359 1103 48-49 0.4681216406326527 1103 50-51 0.48374322954718707 1103 52-53 0.3644832789068815 1103 54-55 0.4192708069980142 1103 56-57 0.42314852341554143 1103 58-59 0.3819809917201127 1103 60-61 0.37182407696376885 1103 62-63 0.46418714534304684 1103 64-65 0.5582312854322709 1103 66-67 0.48554735749350186 1103 68-69 0.4754126825809024 1103 70-71 0.5440144875801352 1103 72-73 0.52540148377647 1103 74-75 0.5089142351281808 1103 76-77 0.39679660926385907 1103 78-79 0.49631704093665263 1103 80-81 0.42608932058180216 1103 82-83 0.4384335775333028 1103 84-85 0.46525751178290875 1103 86-87 0.46661020954386245 1103 88-89 0.5094617678619819 1103 90-91 0.35676714419944133 1103 92-93 0.4100214693800517 1103 94-95 0.4307760694420075 1103 96-97 0.42111539794068875 1103 98-99 0.413115406578175 1103 100-101 0.3633346791421914 1103 102-103 0.15303581594967852 1103 104-105 0.34971875467724445 1103 106-107 0.36398264661821145 1103 108-109 0.3674350713767929 1103 110-111 0.34953408286040855 1103 112-113 0.2678208222591749 1103 114-115 0.3403483394579645 1103 116-117 0.28052333356637504 1103 118-119 0.27107810988936265 1103 120-121 0.0742860462180559 1103 122-123 0.016418986406343095 1103 124-125 0.054782560588037654 1104 1 0.4749349278617139 1104 2 0.44864086209285503 1104 3 0.44710079252730495 1104 4 0.5604792787103179 1104 5 0.45284711530798205 1104 6 0.4211088031096537 1104 7 0.4588046840656972 1104 8 0.49175036201123135 1104 9 0.7233988326951319 1104 10-11 0.659593754097358 1104 12-13 0.6024322988476882 1104 14-15 0.6797934025857444 1104 16-17 0.712659612287176 1104 18-19 0.652894562778279 1104 20-21 0.6812402662573902 1104 22-23 0.6764325909086892 1104 24-25 0.6270975980012778 1104 26-27 0.709062400056375 1104 28-29 0.6238260088481198 1104 30-31 0.6935876754608614 1104 32-33 0.6580215813023571 1104 34-35 0.6856294404557985 1104 36-37 0.6949025581352544 1104 38-39 0.6488922365540333 1104 40-41 0.7042741861778197 1104 42-43 0.5010844639442595 1104 44-45 0.4847015509042123 1104 46-47 0.49324735681370413 1104 48-49 0.5194452034592842 1104 50-51 0.46852108752064225 1104 52-53 0.41331242201606955 1104 54-55 0.39002277513360184 1104 56-57 0.3744396738225362 1104 58-59 0.34908906876142964 1104 60-61 0.25260317797022935 1104 62-63 0.29548718410428876 1104 64-65 0.40787942689744483 1104 66-67 0.46749831783673557 1104 68-69 0.2933267261806236 1104 70-71 0.1338754149094754 1104 72-73 0.2057949772338361 1104 74-75 0.17460017548932427 1104 76-77 0.30872838947423986 1104 78-79 0.3865091095841464 1104 80-81 0.39561162166310027 1104 82-83 0.3882611568267862 1104 84-85 0.22234313403453143 1104 86-87 0.39961006251845177 1104 88-89 0.4453687141442444 1104 90-91 0.3582674716651191 1104 92-93 0.30204968489256956 1104 94-95 0.2325741233602372 1104 96-97 0.22210481211126165 1104 98-99 0.29804463389282176 1104 100-101 0.29676057165325176 1104 102-103 0.11109514738505055 1104 104-105 0.15888478646146353 1104 106-107 0.24094242848596537 1104 108-109 0.2694793928545316 1104 110-111 0.28788532656170673 1104 112-113 0.26125814265427394 1104 114-115 0.14405772368882452 1104 116-117 0.057455715244334016 1104 118-119 0.1748115528234564 1104 120-121 0.20686286699408285 1104 122-123 0.11290441084196345 1104 124-125 0.10111895472306287 1105 1 0.2621537417676443 1105 2 0.21750589276770071 1105 3 0.2841183027522547 1105 4 0.3581275404690061 1105 5 0.3338894210339518 1105 6 0.36988180924462455 1105 7 0.30675330369759735 1105 8 0.2545603057740138 1105 9 0.4022651414885843 1105 10-11 0.4517173481668877 1105 12-13 0.4403652615266296 1105 14-15 0.6629685687411637 1105 16-17 0.503302186418054 1105 18-19 0.4199270908968842 1105 20-21 0.4228238552144461 1105 22-23 0.384428820458794 1105 24-25 0.479519632766106 1105 26-27 0.48180652071077645 1105 28-29 0.4695219444309373 1105 30-31 0.4399977598166913 1105 32-33 0.489321108398471 1105 34-35 0.36758879628401786 1105 36-37 0.26370687817615135 1105 38-39 0.40057775547019503 1105 40-41 0.43070567902035606 1105 42-43 0.3772124036170652 1105 44-45 0.28142157902282605 1105 46-47 0.3157805878975495 1105 48-49 0.1554769647067218 1105 50-51 0.28432023118116945 1105 52-53 0.22263789491995567 1105 54-55 0.30216330427469984 1105 56-57 0.24403802504951955 1105 58-59 0.17526973082686936 1105 60-61 0.1944088400356634 1105 62-63 0.14901319381799283 1105 64-65 0.18640224131462446 1105 66-67 0.10957717775494302 1105 68-69 0.22355742658962185 1105 70-71 0.15145593638187194 1105 72-73 0.09993001046492367 1105 74-75 0.08980339634822343 1105 76-77 0.13706587669305748 1105 78-79 0.2785831765575679 1105 80-81 0.2298236615404008 1105 82-83 0.22713353668363112 1105 84-85 0.1479406544844295 1105 86-87 0.15315364636303386 1105 88-89 0.2113595755961839 1105 90-91 0.13695164855673525 1105 92-93 0.23784179890074952 1105 94-95 0.18211559830911384 1105 96-97 0.23140951558774958 1105 98-99 0.21246181180693213 1105 100-101 0.14302047707247567 1105 102-103 0.03633000167952716 1105 104-105 0.1693334060933651 1105 106-107 0.17691667439599001 1105 108-109 0.220236678130604 1105 110-111 0.2644510218582319 1105 112-113 0.22933872803259803 1105 114-115 0.1816967190875971 1105 116-117 0.19243782158380185 1105 118-119 0.2766728130484033 1105 120-121 0.12955905820062696 1105 122-123 0.07637098804033116 1105 124-125 -0.05920504834442397 1106 1 0.4888553124482726 1106 2 0.46091776013244967 1106 3 0.407795767848242 1106 4 0.519296824936724 1106 5 0.3910911007896303 1106 6 0.4262517917926161 1106 7 0.3885432251635663 1106 8 0.397849463714671 1106 9 0.4136471222913798 1106 10-11 0.523458731238442 1106 12-13 0.4736399670240061 1106 14-15 0.49909285975338236 1106 16-17 0.6612714817932712 1106 18-19 0.673841521525155 1106 20-21 0.4367472845338227 1106 22-23 0.5378960434081819 1106 24-25 0.477692625785302 1106 26-27 0.6370693915659231 1106 28-29 0.6264563903297216 1106 30-31 0.6505821293629381 1106 32-33 0.6272096882414004 1106 34-35 0.6015929411356709 1106 36-37 0.5879624410033841 1106 38-39 0.44209853426600887 1106 40-41 0.5811856092123335 1106 42-43 0.47750417935877465 1106 44-45 0.49269039446785 1106 46-47 0.5048839910389091 1106 48-49 0.5038885593838671 1106 50-51 0.32294779444470123 1106 52-53 0.46362693287807843 1106 54-55 0.5476325996499156 1106 56-57 0.4487841528854801 1106 58-59 0.4389709197448468 1106 60-61 0.38183930469534744 1106 62-63 0.33560378064173335 1106 64-65 0.32562426356593477 1106 66-67 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-0.7028671125464996 2216 52-53 -0.7788358647242788 2216 54-55 -0.6665484090057703 2216 56-57 -0.773659704090349 2216 58-59 -0.9240405501061204 2216 60-61 -0.8985090098357489 2216 62-63 -0.9213148217913769 2216 64-65 -0.7584116383467503 2216 66-67 -0.9495596185731046 2216 68-69 -0.8560063950277623 2216 70-71 -0.7952333227980048 2216 72-73 -0.6147175061445935 2216 74-75 -0.7121955499298167 2216 76-77 -0.6110257033541018 2216 78-79 -0.7743637846154598 2216 80-81 -0.7505942999540949 2216 82-83 -0.6944040267182814 2216 84-85 -0.86538379365102 2216 86-87 -0.879042694185614 2216 88-89 -0.892820374218072 2216 90-91 -0.8777395126805807 2216 92-93 -0.9648189606720479 2216 94-95 -0.8738172804950004 2216 96-97 -0.8800778397487292 2216 98-99 -0.9530296869786063 2216 100-101 -0.8353805668266503 2216 102-103 -0.9108067544642076 2216 104-105 -0.8876233913062634 2216 106-107 -0.7724237447710394 2216 108-109 -0.7350826085532098 2216 110-111 -0.6729878086889869 2216 112-113 -0.6487343672310146 2216 114-115 -0.637233775447342 2216 116-117 -0.8110189222367445 2216 118-119 -0.6968040899020167 2216 120-121 -0.5340931153241613 2216 122-123 -0.6083075151386979 2216 124-125 -0.4846084541881126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21350.0 3 4625.0 4 3453.0 5 2869.0 6 3244.0 7 3416.0 8 3238.0 9 3250.0 10 3665.0 11 4321.0 12 4691.0 13 4858.0 14 4999.0 15 4586.0 16 4328.0 17 4095.0 18 3488.0 19 3334.0 20 3212.0 21 3271.0 22 3738.0 23 5300.0 24 8893.0 25 12968.0 26 16371.0 27 13728.0 28 14595.0 29 18340.0 30 21453.0 31 26366.0 32 34329.0 33 46293.0 34 66381.0 35 102990.0 36 201581.0 37 412858.0 38 193643.0 39 672.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55303787746499 19.720435225377948 12.43971300221136 23.286813894945706 2 15.060915183726497 21.652484148458022 39.79132890337221 23.495271764443267 3 17.838082483367547 28.204498446813997 29.280606877887607 24.676812191930846 4 11.637538193468261 17.41390443059679 38.19671219503153 32.75184518090342 5 12.27043959566932 41.25038142581799 33.806648976233795 12.672530002278897 6 30.106577356464364 38.596995437917435 18.11451947480038 13.181907730817812 7 27.830958111026327 34.43027103176197 21.51324264958031 16.2255282076314 8 25.97251141496086 36.90330184307595 20.023370549092053 17.100816192871132 9 27.28500021625095 17.183300483784265 19.515335281651417 36.01636401831337 10-11 23.863454252602452 29.110909533943545 27.596454867760357 19.429181345693642 12-13 24.232959679698975 26.827875271667356 27.74536562911749 21.193799419516182 14-15 21.890880978198748 27.617957534360837 25.111948828688558 25.37921265875186 16-17 21.661921368746526 31.042550899160908 25.811048196600144 21.484479535492422 18-19 21.75111658947274 29.80411510429096 27.254977830398886 21.189790475837412 20-21 22.861132468046012 28.98130702896953 26.937116902605556 21.220443600378903 22-23 22.55985715069073 29.076816258376837 26.790655789235483 21.57267080169695 24-25 22.097835019060977 29.23457976261824 26.91853208855167 21.749053129769106 26-27 22.053396241788736 29.22134567611851 26.892283429417013 21.83297465267574 28-29 22.039253545465957 28.913547589855476 27.177278124788817 21.86992073988975 30-31 21.9991658879046 28.798569652221595 27.201167756933557 22.001096702940245 32-33 21.7060211671309 29.142784102910856 27.136822520708947 22.014372209249295 34-35 22.25009480367722 29.098704723038104 26.881727489050274 21.7694729842344 36-37 22.3001713404601 28.66803548527735 26.73447943895171 22.297313735310848 38-39 21.94726557974652 28.885881108133365 27.086052564508527 22.08080074761158 40-41 22.08711629291779 28.572741529201917 27.459816734264685 21.880325443615607 42-43 21.951091418407565 28.500683126052785 27.182508574795932 22.365716880743722 44-45 21.816303636353105 28.765285021113744 27.101363366923533 22.317047975609615 46-47 22.1346147756665 28.84484831914958 26.785867371645306 22.234669533538618 48-49 21.952792417623833 28.865447114285537 26.86987562481078 22.31188484327985 50-51 21.91726547584477 28.596060216621666 27.002252099178865 22.4844222083547 52-53 22.05805006105289 28.572366403841894 27.040637823134293 22.328945711970924 54-55 21.85791167983787 28.607238187626276 27.113699696619562 22.42115043591629 56-57 21.88598151051522 28.77416415017107 27.03824558422601 22.3016087550877 58-59 21.966602086915824 28.371389303909577 27.134501072188627 22.52750753698597 60-61 21.95785216902383 28.634219355317057 27.160682620005318 22.247245855653798 62-63 21.98241323529355 28.54773453878862 27.137456889410984 22.332395336506845 64-65 21.840318419364667 28.403141715076146 27.07000970442252 22.686530161136666 66-67 21.855370321025987 28.783823966696403 26.808778494773144 22.55202721750447 68-69 21.675053765350977 28.55705730812943 27.177057439429262 22.59083148709033 70-71 21.84415348701601 28.426995580287578 27.115061198246014 22.613789734450393 72-73 21.95830342106269 28.424644441415136 26.829713803771128 22.78733833375104 74-75 21.772676895035005 28.579085780045027 26.925503855912076 22.72273346900789 76-77 21.896816049120503 28.480353729412442 27.196810256608288 22.42601996485876 78-79 21.917790233100934 28.240076740031554 27.213825563173433 22.62830746369407 80-81 21.890328435442452 28.770017956787857 26.858334459655154 22.481319148114537 82-83 22.131554913663106 28.431223004635914 26.916644301262956 22.52057778043803 84-85 22.12719568391497 28.155071365157674 26.92683608739524 22.79089686353212 86-87 21.97308533866148 28.1511462750983 27.23147705480325 22.644291331436968 88-89 22.281579418925098 28.131217637390478 27.083951235185804 22.503251708498624 90-91 22.22144560400838 28.356236061902052 27.010532616355963 22.411785717733604 92-93 22.357788252339976 27.98366050678997 26.99337414859856 22.665177092271488 94-95 22.074663735920854 27.762161636140853 27.201525202959427 22.961649424978866 96-97 22.22012836058357 27.729593215888293 27.180508036052174 22.86977038747596 98-99 22.11349945917516 27.81681696873179 27.102179016305065 22.967504555787986 100-101 21.840399206653238 27.952922840230837 27.27577450168679 22.930903451429135 102-103 22.204012216836883 27.779131514040518 27.130058956394205 22.886797312728394 104-105 21.992801096402054 28.063521191429746 27.08374122100232 22.85993649116588 106-107 21.91899324512462 27.971965587248576 27.19846264891061 22.91057851871619 108-109 21.9952184391691 28.169000486960766 26.813928854298325 23.021852219571805 110-111 21.87825346856435 28.537611255109006 26.753899528256714 22.83023574806993 112-113 21.833321168912065 28.47926584049007 27.03760814267051 22.649804847927356 114-115 21.961279828600315 28.541384196117942 26.834879125453803 22.66245684982794 116-117 21.836537510967215 28.568708834323985 26.872536238152318 22.72221741655648 118-119 21.731232107188806 28.62189133533978 26.596029099844305 23.050847457627118 120-121 21.719235421613416 28.720584077823485 26.686515446207466 22.873665054355634 122-123 21.932543412559692 28.754395707518714 26.707982906075323 22.605077973846264 124-125 22.0920816201694 28.757443019076607 26.582936443114463 22.567538917639535 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4040.0 1 3195.0 2 2138.0 3 1546.5 4 953.5 5 689.5 6 605.5 7 575.5 8 604.5 9 603.5 10 514.5 11 421.5 12 398.0 13 415.0 14 459.5 15 512.5 16 537.5 17 575.0 18 779.0 19 1026.5 20 1245.5 21 1676.5 22 2231.5 23 3058.5 24 4002.0 25 5148.5 26 6843.0 27 8483.5 28 9802.0 29 11602.5 30 14728.5 31 17934.5 32 21431.5 33 25318.5 34 29245.0 35 32099.5 36 36503.5 37 41410.0 38 43091.5 39 44532.5 40 44488.0 41 44572.0 42 44367.5 43 45419.5 44 48231.0 45 49381.5 46 48490.0 47 47035.5 48 46812.5 49 45569.0 50 44495.0 51 42846.5 52 41202.0 53 40581.5 54 39233.5 55 36756.5 56 33446.0 57 30249.0 58 27611.5 59 24515.5 60 20083.5 61 15885.0 62 12921.0 63 10336.0 64 8133.0 65 6324.5 66 4671.0 67 3848.5 68 2740.0 69 1458.0 70 994.5 71 747.5 72 571.5 73 506.5 74 399.5 75 296.5 76 227.5 77 150.5 78 76.5 79 40.0 80 28.5 81 20.0 82 13.0 83 9.0 84 6.0 85 4.5 86 3.5 87 4.0 88 4.5 89 3.5 90 2.5 91 4.0 92 3.5 93 2.5 94 1.5 95 1.5 96 2.0 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04371358488467646 2 0.009499595301793647 3 0.0026259043923657238 4 0.0052518087847314475 5 0.023710372013419915 6 0.032978269868828354 7 0.002780369356622531 8 0.0 9 0.0 10-11 1.930812053210091E-4 12-13 7.723248212840365E-4 14-15 3.089299285136145E-4 16-17 1.1584872319260546E-4 18-19 7.723248212840363E-5 20-21 3.8616241064201814E-5 22-23 7.723248212840363E-5 24-25 0.0 26-27 3.8616241064201814E-5 28-29 3.4754616957781637E-4 30-31 1.5446496425680726E-4 32-33 1.1584872319260546E-4 34-35 8.495573034124399E-4 36-37 1.1584872319260546E-4 38-39 1.5446496425680726E-4 40-41 4.2477865170621997E-4 42-43 6.950923391556327E-4 44-45 0.0015060334015038709 46-47 7.723248212840363E-5 48-49 0.0 50-51 0.0 52-53 0.0010040222676692473 54-55 0.0037071591421633746 56-57 1.5446496425680726E-4 58-59 8.109410623482381E-4 60-61 0.001235719714054458 62-63 8.881735444766419E-4 64-65 0.0016604983657606783 66-67 0.0028576018387509346 68-69 0.003514077936842365 70-71 0.0018921958121458891 72-73 0.0025872881513015217 74-75 0.002085277017466898 76-77 0.0013129521961828619 78-79 0.002394206945980513 80-81 0.0013129521961828619 82-83 7.337085802198345E-4 84-85 5.792436159630272E-4 86-87 0.00254867191023732 88-89 0.0067964584272995196 90-91 3.4754616957781637E-4 92-93 0.0011584872319260545 94-95 0.001042638508733449 96-97 1.5446496425680726E-4 98-99 8.109410623482381E-4 100-101 0.0018535795710816873 102-103 0.0016604983657606783 104-105 8.881735444766419E-4 106-107 0.007530167007519355 108-109 0.0020466607764026965 110-111 0.001544649642568073 112-113 0.002510055669173118 114-115 0.0031279155262003474 116-117 0.0018535795710816873 118-119 0.04842476629450908 120-121 0.013129521961828617 122-123 0.004865646374089429 124-125 0.0029734505619435398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1294792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.9822393976455 #Duplication Level Percentage of deduplicated Percentage of total 1 79.39135391884179 42.063317194312766 2 11.026524128169966 11.68419882165231 3 3.7331842700858195 5.933773881396341 4 1.7504298827645808 3.7096678038970223 5 0.9855311931933215 2.610782480580789 6 0.6009994777651785 1.9105378925288767 7 0.4027279758216154 1.4936201018977011 8 0.2963959028820107 1.2562974946380783 9 0.21796310973488642 1.0393356293846125 >10 1.3519221382705222 13.899915164500875 >50 0.1508400366128443 5.58783691554002 >100 0.08962958034265398 7.9843965235441665 >500 0.0024983855147777396 0.8263200961263882 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.723248212840363E-5 0.0 18-19 0.0 0.0 0.0 7.723248212840363E-5 0.0 20-21 0.0 0.0 0.0 1.5446496425680726E-4 0.0 22-23 0.0 0.0 0.0 2.703136874494127E-4 0.0 24-25 0.0 0.0 0.0 5.792436159630272E-4 0.0 26-27 0.0 0.0 0.0 0.0014674171604396689 0.0 28-29 0.0 0.0 0.0 0.00509734382047464 0.0 30-31 0.0 0.0 0.0 0.02081415393360478 0.0 32-33 0.0 0.0 0.0 0.05062589203516858 0.0 34-35 0.0 0.0 0.0 0.09314237344685478 0.0 36-37 0.0 0.0 0.0 0.15859690205067686 0.0 38-39 0.0 0.0 0.0 0.26092994087081167 0.0 40-41 0.0 0.0 0.0 0.37878670859875563 0.0 42-43 0.0 0.0 0.0 0.49143028378303233 0.0 44-45 0.0 0.0 0.0 0.6209877725534294 0.0 46-47 0.0 0.0 0.0 0.7785806523364371 0.0 48-49 0.0 0.0 0.0 0.9575669296690125 0.0 50-51 0.0 0.0 0.0 1.154355294132185 0.0 52-53 0.0 0.0 0.0 1.343806572793159 0.0 54-55 0.0 0.0 0.0 1.538239346551415 0.0 56-57 0.0 0.0 0.0 1.766499947481912 0.0 58-59 0.0 0.0 0.0 2.022139463326928 0.0 60-61 0.0 0.0 0.0 2.3129197585403682 0.0 62-63 0.0 0.0 0.0 2.592733041291574 0.0 64-65 0.0 0.0 0.0 2.86822130504359 0.0 66-67 0.0 0.0 0.0 3.177074001075076 0.0 68-69 0.0 0.0 0.0 3.5322661863836045 0.0 70-71 0.0 0.0 0.0 3.9260359965152705 0.0 72-73 0.0 0.0 0.0 4.332665014921316 0.0 74-75 0.0 0.0 0.0 4.7291765781685395 0.0 76-77 0.0 0.0 0.0 5.140555394225482 0.0 78-79 0.0 0.0 0.0 5.587770081990003 0.0 80-81 0.0 0.0 0.0 6.097234150350017 0.0 82-83 0.0 0.0 0.0 6.6416845331142 0.0 84-85 0.0 0.0 0.0 7.1590649308923755 0.0 86-87 0.0 0.0 0.0 7.67285401825158 0.0 88-89 0.0 0.0 0.0 8.230472539218656 0.0 90-91 0.0 0.0 0.0 8.865709704724775 0.0 92-93 0.0 0.0 0.0 9.52481170720857 0.0 94-95 0.0 0.0 0.0 10.183797860969175 0.0 96-97 0.0 0.0 0.0 10.81706559818102 0.0 98-99 0.0 0.0 0.0 11.49327459545626 0.0 100-101 0.0 0.0 0.0 12.226288083336938 0.0 102-103 0.0 0.0 0.0 12.997995044763947 0.0 104-105 0.0 0.0 0.0 13.774799350011431 0.0 106-107 0.0 0.0 0.0 14.526039703674412 0.0 108-109 0.0 0.0 0.0 15.27098561004393 0.0 110-111 0.0 0.0 0.0 16.071345822340575 0.0 112-113 0.0 0.0 0.0 16.9383962829551 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1590 0.0 62.902607 1 GTATCAA 2655 0.0 53.590755 1 ATCAACG 3170 0.0 43.37023 3 TCAACGC 3155 0.0 43.199146 4 GTACATG 8515 0.0 41.52965 1 CAACGCA 3305 0.0 41.23852 5 TATCAAC 3560 0.0 39.45491 2 AACGCAG 3465 0.0 38.986237 6 TACATGG 9145 0.0 37.747078 2 ACATGGG 9095 0.0 36.38406 3 ACGCAGA 4150 0.0 32.544876 7 CGCAGAG 4155 0.0 32.361263 8 CATGGGA 7185 0.0 32.139862 4 CATGGGG 2200 0.0 30.840487 4 CGACGGC 80 0.0053581223 29.754915 6 ATGGGAT 3075 0.0 27.484148 5 AGTACAT 5890 0.0 27.383757 2 GAGTACA 6350 0.0 27.000713 1 GCAGAGT 4905 0.0 26.68528 9 ATGGGAG 2400 0.0 25.790583 5 >>END_MODULE