FastQCFastQC Report
Thu 26 May 2016
SRR1364402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294792
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79160.6113723285284433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66120.5106611718330049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.3319452081878788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.11600318815686225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.10990182206871837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31400.083.638871
GTATCAA64100.061.5963131
ATCAACG85050.046.269213
TCAACGC85100.046.2420274
TATCAAC86550.045.3985332
CAACGCA87300.045.0767065
AACGCAG90050.043.7459566
TATAACG1109.584255E-943.2974432
ACGCAGA103150.038.184347
GTACATG87250.038.1544841
GTGGTAT11500.037.8028221
CGCAGAG104200.037.7424668
TACATGG91800.035.6036572
ACATGGG90850.035.255133
GCAGAGT116300.033.7645349
CATGGGA70800.032.625974
TGGTATC12800.032.0925372
CATGGGG23950.030.5748674
CAGAGTA113700.028.38806510-11
ATGGGAT32800.028.3149455
Produced by FastQC (version 0.11.5)