##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364402_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1294792 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29592243387355 33.0 31.0 34.0 30.0 34.0 2 32.29894608554888 33.0 31.0 34.0 30.0 34.0 3 32.39824697712065 34.0 31.0 34.0 30.0 34.0 4 36.01766152401312 37.0 35.0 37.0 35.0 37.0 5 35.96879653257048 37.0 35.0 37.0 35.0 37.0 6 36.03808101996305 37.0 35.0 37.0 35.0 37.0 7 35.98689828173174 37.0 35.0 37.0 35.0 37.0 8 36.00793023126494 37.0 35.0 37.0 35.0 37.0 9 37.75558004683378 39.0 38.0 39.0 35.0 39.0 10-11 37.70924789464254 39.0 37.5 39.0 35.0 39.0 12-13 37.678019326656326 39.0 37.0 39.0 35.0 39.0 14-15 39.025275874426164 41.0 38.0 41.0 35.5 41.0 16-17 39.05377968044289 41.0 38.0 41.0 35.5 41.0 18-19 39.145464676951974 41.0 38.5 41.0 36.0 41.0 20-21 39.16250679645843 41.0 39.0 41.0 35.5 41.0 22-23 39.101468807345114 41.0 39.0 41.0 35.0 41.0 24-25 39.06067963039624 41.0 39.0 41.0 35.0 41.0 26-27 38.953770180847584 41.0 38.5 41.0 35.0 41.0 28-29 38.84806941964423 40.5 38.0 41.0 35.0 41.0 30-31 38.68741427194483 40.0 38.0 41.0 35.0 41.0 32-33 38.49778844787426 40.0 38.0 41.0 34.0 41.0 34-35 38.37583797243109 40.0 38.0 41.0 34.0 41.0 36-37 38.232445829137035 40.0 38.0 41.0 34.0 41.0 38-39 38.14078940864633 40.0 38.0 41.0 34.0 41.0 40-41 38.01491706776069 40.0 38.0 41.0 33.0 41.0 42-43 37.82752210393639 40.0 37.5 41.0 33.0 41.0 44-45 37.587385464229 40.0 37.0 41.0 33.0 41.0 46-47 37.22683720628487 40.0 37.0 41.0 31.5 41.0 48-49 36.92499606114342 40.0 37.0 41.0 31.0 41.0 50-51 36.773122632824425 40.0 37.0 41.0 31.0 41.0 52-53 36.643843953314516 40.0 36.0 41.0 31.0 41.0 54-55 36.47679897620622 40.0 36.0 41.0 30.0 41.0 56-57 36.30199174848161 39.5 35.5 41.0 30.0 41.0 58-59 36.09853899313558 39.0 35.0 41.0 29.5 41.0 60-61 35.85357262015829 39.0 35.0 41.0 29.0 41.0 62-63 35.62602835049954 38.5 35.0 41.0 29.0 41.0 64-65 35.343382566466275 38.0 35.0 40.0 28.5 41.0 66-67 35.017310116219434 37.0 35.0 40.0 28.0 41.0 68-69 34.67985166729483 37.0 34.5 39.5 28.0 41.0 70-71 34.41606296609803 36.0 34.0 39.0 28.0 41.0 72-73 34.1964809791843 36.0 34.0 39.0 28.0 41.0 74-75 33.854671252216576 35.5 34.0 38.5 28.0 40.5 76-77 33.518245787740426 35.0 34.0 37.0 28.0 39.5 78-79 33.19766920092185 35.0 34.0 37.0 27.0 39.0 80-81 32.88926677026117 35.0 34.0 36.5 26.5 39.0 82-83 32.60580000494288 35.0 34.0 36.0 26.0 37.5 84-85 32.34855250881995 35.0 34.0 36.0 26.0 37.0 86-87 32.15578563970121 35.0 33.0 35.0 26.0 37.0 88-89 31.984461596920585 35.0 33.0 35.0 26.0 36.0 90-91 31.829920944831294 35.0 33.0 35.0 25.0 36.0 92-93 31.69961854877077 35.0 33.0 35.0 25.0 36.0 94-95 31.608866906808196 35.0 33.0 35.0 25.0 36.0 96-97 31.517607847438043 35.0 33.0 35.0 25.0 35.0 98-99 31.44916364945103 35.0 33.0 35.0 25.0 35.0 100-101 31.384004921253762 35.0 33.0 35.0 24.0 35.0 102-103 30.422453567831745 34.0 31.5 35.0 21.5 35.0 104-105 31.119018344259153 35.0 32.5 35.0 23.5 35.0 106-107 31.38789550754098 35.0 33.0 35.0 24.5 35.0 108-109 31.3914269627863 35.0 33.0 35.0 24.5 35.0 110-111 31.35285860586102 35.0 33.0 35.0 24.0 35.0 112-113 31.279557257072952 35.0 33.0 35.0 24.0 35.0 114-115 31.19405742389511 35.0 33.0 35.0 23.0 35.0 116-117 31.122626645824194 35.0 33.0 35.0 23.0 35.0 118-119 31.007538276418146 35.0 33.0 35.0 19.5 35.0 120-121 30.861666584285352 35.0 33.0 35.0 18.5 35.0 122-123 30.704312739034535 35.0 33.0 35.0 8.5 35.0 124-125 30.034222871318327 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15676106323992656 1101 2 0.1828724901943275 1101 3 0.16403480702718554 1101 4 0.15253629285620462 1101 5 0.2801110676856311 1101 6 0.22760670189670407 1101 7 0.2593991911074127 1101 8 0.20505223020546026 1101 9 0.22347407534360997 1101 10-11 0.30856186857823786 1101 12-13 0.2961263933662579 1101 14-15 0.39264635137011794 1101 16-17 0.3717591087294849 1101 18-19 0.36370090893307605 1101 20-21 0.33541660963021513 1101 22-23 0.35493871385380515 1101 24-25 0.40986000582402937 1101 26-27 0.38242971417844984 1101 28-29 0.27182808370351097 1101 30-31 0.3278464717261613 1101 32-33 0.22718134030376547 1101 34-35 0.23777032362799844 1101 36-37 0.3760292944560675 1101 38-39 0.37604517569596396 1101 40-41 0.34501007968093944 1101 42-43 0.3972423738976971 1101 44-45 0.3836591052073075 1101 46-47 0.41680744563760186 1101 48-49 0.2173662444471276 1101 50-51 0.2522745761609215 1101 52-53 0.30734173029513556 1101 54-55 0.29463136948333357 1101 56-57 0.30461079226414967 1101 58-59 0.15379902276426805 1101 60-61 0.2239784883964262 1101 62-63 0.1348059980732188 1101 64-65 0.08324043497704281 1101 66-67 0.09902750576615205 1101 68-69 -0.009397688152191108 1101 70-71 0.14677952724326815 1101 72-73 0.06811086836305691 1101 74-75 0.16850583935387675 1101 76-77 0.07736078704625982 1101 78-79 -0.030650040803472223 1101 80-81 -0.028524628662744078 1101 82-83 0.06308081181796155 1101 84-85 -0.1672569169020761 1101 86-87 -0.1518456638098371 1101 88-89 -0.19064673561991796 1101 90-91 -0.5452907083678262 1101 92-93 -0.4873727324886481 1101 94-95 -0.2833198634601928 1101 96-97 -0.08021695849715016 1101 98-99 -0.0026236741638605565 1101 100-101 -0.050222801031797104 1101 102-103 0.10365533028774365 1101 104-105 -0.0019762027749372635 1101 106-107 0.07259745854186761 1101 108-109 0.12877915094582448 1101 110-111 0.07791573177806654 1101 112-113 0.09631630063212882 1101 114-115 0.012800050439714994 1101 116-117 0.014927252613631481 1101 118-119 0.0033255179965863135 1101 120-121 -0.037597531214562 1101 122-123 -0.051506775969780705 1101 124-125 0.017081737184319934 1102 1 0.1469408026804615 1102 2 0.19249136202491002 1102 3 0.12065089610926805 1102 4 0.13438741367835405 1102 5 0.005631883685452976 1102 6 0.06568504381892382 1102 7 0.15029343386445504 1102 8 0.2568142535819149 1102 9 0.25233239751949554 1102 10-11 0.27485613676488185 1102 12-13 0.2439799393865698 1102 14-15 0.31312169703765846 1102 16-17 0.39950083280474047 1102 18-19 0.3906716384466833 1102 20-21 0.40135008478900147 1102 22-23 0.3865759444857204 1102 24-25 0.3653727236075923 1102 26-27 0.32850276196645467 1102 28-29 0.315548211397612 1102 30-31 0.4348030869618995 1102 32-33 0.3824371167895819 1102 34-35 0.3433935918769322 1102 36-37 0.39499567533847113 1102 38-39 0.37299021107979513 1102 40-41 0.39099711058813824 1102 42-43 0.41699724285422235 1102 44-45 0.47212209106828595 1102 46-47 0.4479966716819348 1102 48-49 0.6262480847304985 1102 50-51 0.4740206335072159 1102 52-53 0.5347834192980372 1102 54-55 0.6143086696455526 1102 56-57 0.5474510389482745 1102 58-59 0.5099170160791857 1102 60-61 0.42807700503189494 1102 62-63 0.4778375640497856 1102 64-65 0.36228627907315314 1102 66-67 0.42985713346153176 1102 68-69 0.3400518635445451 1102 70-71 0.2839478651803802 1102 72-73 0.441924616215843 1102 74-75 0.37999216497384936 1102 76-77 0.3829664764485159 1102 78-79 0.30008486592196704 1102 80-81 0.4372089937828889 1102 82-83 0.3317299627852677 1102 84-85 0.43646553188246173 1102 86-87 0.41568612104897795 1102 88-89 0.2858756906363915 1102 90-91 0.37956057544264965 1102 92-93 0.4101835518210386 1102 94-95 0.32390043447565375 1102 96-97 0.35095588337941663 1102 98-99 0.46522538327715424 1102 100-101 0.4680557861409369 1102 102-103 0.5934950129127614 1102 104-105 0.4458376628390468 1102 106-107 0.3414744518422772 1102 108-109 0.288100249060534 1102 110-111 0.31032429830845487 1102 112-113 0.29386115015805103 1102 114-115 0.2081535175258864 1102 116-117 0.17823175151836423 1102 118-119 0.17653219829966105 1102 120-121 0.15795305789013625 1102 122-123 0.12219398939272352 1102 124-125 0.0978113105218199 1103 1 0.1410596736995302 1103 2 0.16240204789725965 1103 3 0.14003318085837435 1103 4 0.1388102814521801 1103 5 -0.043285436051426984 1103 6 0.04603348781574823 1103 7 0.11591064204611001 1103 8 0.17688615250377637 1103 9 0.18630673631868433 1103 10-11 0.19766314970738108 1103 12-13 0.2654311540796854 1103 14-15 0.33253283227827524 1103 16-17 0.27919909163386336 1103 18-19 0.25369606169912373 1103 20-21 0.1398072654140634 1103 22-23 0.25314810393626175 1103 24-25 0.2758457143019939 1103 26-27 0.2112910832874917 1103 28-29 0.23447701887566552 1103 30-31 0.26984384483808554 1103 32-33 0.2601464402193301 1103 34-35 0.24990925681956355 1103 36-37 0.30355141973360134 1103 38-39 0.31835918263845286 1103 40-41 0.30998099627724685 1103 42-43 0.19596619622833344 1103 44-45 0.27330504864581684 1103 46-47 0.2823970360723891 1103 48-49 0.2235621039711546 1103 50-51 0.09168780024906908 1103 52-53 0.19048821057783982 1103 54-55 0.2614278169299382 1103 56-57 0.1924820226276367 1103 58-59 0.2156546794341665 1103 60-61 0.29293299736641387 1103 62-63 0.32419733142739915 1103 64-65 0.16678485753075734 1103 66-67 0.05679011639254128 1103 68-69 0.09624204248753188 1103 70-71 0.24577385650075456 1103 72-73 0.30549763593558765 1103 74-75 0.3773864410363359 1103 76-77 0.3074337040386794 1103 78-79 0.38140784455162446 1103 80-81 0.43319134427614614 1103 82-83 0.3873698841720383 1103 84-85 0.38739434285753305 1103 86-87 0.37746446534549705 1103 88-89 0.36032088694213016 1103 90-91 0.3451240167992218 1103 92-93 0.2969396899816701 1103 94-95 0.22572439751905193 1103 96-97 0.19026651399870786 1103 98-99 0.24834968168565652 1103 100-101 0.13856262807870934 1103 102-103 0.3146650664522781 1103 104-105 0.33380289887103487 1103 106-107 0.2797007411095471 1103 108-109 0.26168587557081224 1103 110-111 0.28196994782918594 1103 112-113 0.3924314771652604 1103 114-115 0.44651933826116874 1103 116-117 0.40541494689389523 1103 118-119 0.22600379717641417 1103 120-121 0.26275115690502204 1103 122-123 0.2785859469248031 1103 124-125 0.12890820620892995 1104 1 0.10548954926257181 1104 2 0.03953892855272301 1104 3 0.1184204457847926 1104 4 0.0753387442346991 1104 5 0.1570333417664429 1104 6 0.15242641990337802 1104 7 0.19075994100222715 1104 8 0.19679807091804236 1104 9 0.24310532487476166 1104 10-11 0.2755097621083067 1104 12-13 0.19273210172527655 1104 14-15 0.24333519003118909 1104 16-17 0.22634345721922955 1104 18-19 0.2501774763508777 1104 20-21 0.17824104383340256 1104 22-23 0.2206404519320344 1104 24-25 0.18946995745528739 1104 26-27 0.08753411628801189 1104 28-29 0.15439161047918049 1104 30-31 0.2659794156308237 1104 32-33 0.19946838576646542 1104 34-35 0.19236551676048208 1104 36-37 0.3491627112849969 1104 38-39 0.24577207008381663 1104 40-41 0.22240731649858958 1104 42-43 0.22098390610710084 1104 44-45 0.15846316778986846 1104 46-47 0.18127362829073235 1104 48-49 0.1889275957711476 1104 50-51 0.17493759641838125 1104 52-53 0.19848271717393118 1104 54-55 0.23853195101710867 1104 56-57 0.2570378722340081 1104 58-59 0.3561107255199474 1104 60-61 0.3533236839750771 1104 62-63 0.2424044233984759 1104 64-65 0.24863958306104905 1104 66-67 0.2075379410847873 1104 68-69 0.1880677639144821 1104 70-71 0.22171682400814063 1104 72-73 0.468807779352197 1104 74-75 0.4140709512412357 1104 76-77 0.34353846632532736 1104 78-79 0.3789117540909004 1104 80-81 0.2854575404615005 1104 82-83 0.25241726645147367 1104 84-85 0.31089602028379204 1104 86-87 0.35819745383741264 1104 88-89 0.37728394346671834 1104 90-91 0.39608502833403136 1104 92-93 0.4592891560121153 1104 94-95 0.4130798782253038 1104 96-97 0.37989921779597324 1104 98-99 0.32850526129991664 1104 100-101 0.3464966537680567 1104 102-103 0.37860942401807307 1104 104-105 0.3921185223255996 1104 106-107 0.21980125302529174 1104 108-109 0.25163468399670563 1104 110-111 0.22000542105051224 1104 112-113 0.1955260278689792 1104 114-115 0.1959045047340311 1104 116-117 0.19758283654464392 1104 118-119 0.12096583788908433 1104 120-121 0.0946108946651556 1104 122-123 0.06612253620263786 1104 124-125 -0.14039389090803311 1105 1 0.14720414537708848 1105 2 0.21598320258135573 1105 3 0.13409976582569527 1105 4 0.12766172480729665 1105 5 0.07138980904660741 1105 6 0.1172174475107397 1105 7 0.19064171503085703 1105 8 0.20926292265628632 1105 9 0.2183737297827406 1105 10-11 0.1717712651757921 1105 12-13 0.26012410070277525 1105 14-15 0.2403300136508193 1105 16-17 0.27338141734193044 1105 18-19 0.29269728718931987 1105 20-21 0.2529966036493505 1105 22-23 0.3050442096007444 1105 24-25 0.3871917110340206 1105 26-27 0.28936502119599083 1105 28-29 0.3415848620129154 1105 30-31 0.3857598987596518 1105 32-33 0.28411498597095886 1105 34-35 0.28538379385659596 1105 36-37 0.3803488053545223 1105 38-39 0.39664758133126554 1105 40-41 0.3859154325517551 1105 42-43 0.3089632005856302 1105 44-45 0.3128407240270903 1105 46-47 0.39320007256476686 1105 48-49 0.393394620311021 1105 50-51 0.43563864447564526 1105 52-53 0.37157991809418434 1105 54-55 0.30072520255085067 1105 56-57 0.328075704544851 1105 58-59 0.3272236472990926 1105 60-61 0.2788636890875438 1105 62-63 0.3362854305559395 1105 64-65 0.379662717007875 1105 66-67 0.2993963255428653 1105 68-69 0.3398763017467914 1105 70-71 0.3929671435377884 1105 72-73 0.4307342236262244 1105 74-75 0.4365418740428666 1105 76-77 0.3600038293110117 1105 78-79 0.42353407004181776 1105 80-81 0.3863282587641592 1105 82-83 0.33321513199339137 1105 84-85 0.41677376823879797 1105 86-87 0.3545580110971258 1105 88-89 0.4521832916262234 1105 90-91 0.4343822548166507 1105 92-93 0.5023505696112984 1105 94-95 0.3942020663643646 1105 96-97 0.4195608358941314 1105 98-99 0.39160437939807835 1105 100-101 0.42840808014842935 1105 102-103 0.4725830947747163 1105 104-105 0.4489308945137367 1105 106-107 0.45255783533612615 1105 108-109 0.42311186957952884 1105 110-111 0.4016356613368153 1105 112-113 0.3438101536358502 1105 114-115 0.4010808851021288 1105 116-117 0.23702238153441968 1105 118-119 0.16961422745963972 1105 120-121 0.3051377990618853 1105 122-123 0.36048443548689235 1105 124-125 0.16532568986906426 1106 1 0.17893680244515053 1106 2 0.11981173661276756 1106 3 0.20397596565117482 1106 4 0.15147553370781708 1106 5 0.1852177977028049 1106 6 0.034863062693986535 1106 7 0.07853711206401925 1106 8 0.16859701951492667 1106 9 0.12512547498343451 1106 10-11 0.16682471194717152 1106 12-13 0.141889480283929 1106 14-15 0.18611562665256542 1106 16-17 0.2351478876735129 1106 18-19 0.19302723919630438 1106 20-21 0.14219408401584133 1106 22-23 0.03341735532500678 1106 24-25 0.1459095338961589 1106 26-27 0.19437974632688082 1106 28-29 0.18065554264119044 1106 30-31 0.1781232822675065 1106 32-33 0.14605052262017892 1106 34-35 0.18258329211140278 1106 36-37 0.2553488053545223 1106 38-39 0.18771978901014563 1106 40-41 0.21712861972453368 1106 42-43 0.06684387248790102 1106 44-45 0.10323666643545693 1106 46-47 0.1488324351302026 1106 48-49 0.08903707231799984 1106 50-51 -1.1888170237028817E-4 1106 52-53 -0.12669260590232767 1106 54-55 -0.08646938732698572 1106 56-57 -0.086649426345204 1106 58-59 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46-47 -0.8073775769208709 2216 48-49 -0.9412447031637541 2216 50-51 -0.6762297920867226 2216 52-53 -0.731021234749079 2216 54-55 -0.6764197467276176 2216 56-57 -0.682977660221546 2216 58-59 -0.6853282574373054 2216 60-61 -0.7664479179277421 2216 62-63 -0.8065767193940516 2216 64-65 -0.6697450108818188 2216 66-67 -0.6792586608837681 2216 68-69 -0.750245947860293 2216 70-71 -0.8972326841161617 2216 72-73 -0.9239368677796946 2216 74-75 -0.8846842609406949 2216 76-77 -0.7871205366698462 2216 78-79 -0.6839891029114611 2216 80-81 -0.6144003051378277 2216 82-83 -0.6592930611413372 2216 84-85 -0.5951566191379669 2216 86-87 -0.6279151547122765 2216 88-89 -0.49341954002829524 2216 90-91 -0.6304195249018818 2216 92-93 -0.48840067610237625 2216 94-95 -0.58430647056948 2216 96-97 -0.609565534837401 2216 98-99 -0.6774082697303712 2216 100-101 -0.6686515444407988 2216 102-103 -0.6825529067969356 2216 104-105 -0.6139129046004186 2216 106-107 -0.5471359825307118 2216 108-109 -0.4351782656183545 2216 110-111 -0.45373050704438356 2216 112-113 -0.44610112833300164 2216 114-115 -0.40126855767540803 2216 116-117 -0.5565001306101749 2216 118-119 -0.46105957415488064 2216 120-121 -0.273588068026271 2216 122-123 -0.3926384436744179 2216 124-125 -0.3347319076448656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 200.0 3 9.0 4 46.0 5 160.0 6 374.0 7 734.0 8 1205.0 9 1964.0 10 3418.0 11 4759.0 12 5801.0 13 8631.0 14 13742.0 15 11936.0 16 4952.0 17 4080.0 18 3919.0 19 3688.0 20 3543.0 21 3676.0 22 3721.0 23 4112.0 24 5055.0 25 6540.0 26 8312.0 27 10850.0 28 13756.0 29 17447.0 30 22136.0 31 27691.0 32 35460.0 33 46114.0 34 63428.0 35 98208.0 36 185959.0 37 433267.0 38 234558.0 39 1341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45780079602482 19.299739190625694 13.172959259350819 23.069500753998664 2 15.65432903508826 21.40598644415474 39.31164233328596 23.62804218747104 3 18.01432199148589 27.60003151085271 30.52536623643025 23.860280261231146 4 11.837036373409783 17.1890929199439 38.31472545397253 32.65914525267379 5 11.925657635964752 39.86903305624825 34.5141325758249 13.691176731962102 6 29.872150301992868 38.42157261848943 17.58611180417854 14.120165275339167 7 26.868562672614598 34.44151647523309 21.891778756742394 16.79814209540992 8 26.637096923675774 34.88660726973908 20.17111628740369 18.305179519181458 9 27.77944256683699 14.563265760060304 20.459734073117534 37.19755759998517 10-11 24.31193581671805 27.34547324975749 27.97132666868501 20.37126426483945 12-13 25.481003898695697 25.175510815636798 28.727818831132723 20.615666454534782 14-15 22.604402869341175 25.560283041600506 27.24704045128484 24.58827363777348 16-17 21.04179667467825 29.21056046067631 27.771951016070535 21.975691848574908 18-19 20.976033216145915 26.865203059642013 31.228799683655755 20.92996404055632 20-21 21.886298892599918 26.118801550367838 31.440245692446535 20.554653864585713 22-23 21.679968674505247 26.26611069577198 31.30761543166779 20.746305198054976 24-25 21.26696797632361 26.516961797725042 31.310318568542282 20.905751657409066 26-27 21.158538045253774 26.627468456201676 31.271977553064712 20.942015945479834 28-29 21.089676877479896 26.25577556162691 31.62866493536584 21.025882625527355 30-31 21.15364787376471 26.137280335745956 31.610374976299216 21.098696814190117 32-33 20.91835040303864 26.458558112557334 31.460840337692087 21.162251146711935 34-35 21.4562841730522 26.371063691097042 31.17570939191999 20.996942743930767 36-37 21.509439353965735 26.049705280848194 30.926357283640925 21.514498081545145 38-39 21.259321960592896 26.24618471538286 31.143341942180676 21.351151381843568 40-41 21.35925516879185 26.117555742630078 31.324539114450996 21.19864997412708 42-43 21.376715333428074 26.065576555925585 30.893379013772098 21.664329096874248 44-45 21.324106100562215 26.405623149073147 30.50914414339617 21.761126606968464 46-47 21.73951491822625 26.586857194051245 29.85386842056485 21.81975946715766 48-49 21.806860199067415 26.959018371740534 29.307318768523793 21.92680266066826 50-51 21.774501232630413 26.790596481905975 29.244311055366424 22.19059123009719 52-53 21.797864058474257 26.911195002749476 29.25172537365075 22.03921556512552 54-55 21.579489215256196 27.00120946067013 29.30964973524705 22.109651588826623 56-57 21.633229468396625 27.126241658399564 29.13826599747218 22.102262875731636 58-59 21.66676513827151 26.66900681539354 29.41400899528456 22.250219051050383 60-61 21.674253470827747 26.81565842235664 29.546019746801033 21.964068360014583 62-63 21.622778021489168 26.783722790996546 29.6196223022694 21.973876885244888 64-65 21.376638100945943 26.562413113457605 29.684536203498325 22.37641258209813 66-67 21.40811033741327 26.92776137016602 29.328533077127446 22.335595215293267 68-69 21.303576172852473 26.77974531816693 29.44789587825689 22.468782630723698 70-71 21.388964941459687 26.684450739752307 29.382027917236098 22.54455640155191 72-73 21.42257762935394 26.72432335578613 28.970323770525034 22.882775244334894 74-75 21.235109577445645 26.895478192636347 28.985080229102433 22.884332000815576 76-77 21.35628734190511 26.852845862501468 29.1218975711929 22.66896922440052 78-79 21.327943020963986 26.675558699775713 28.989636945548007 23.006861333712287 80-81 21.240989844677397 27.214434166493533 28.648992229621967 22.8955837592071 82-83 21.4916758830762 26.816392130936862 28.659622549413342 23.0323094365736 84-85 21.38788368629235 26.53265023750928 28.65021125022832 23.42925482597005 86-87 21.21835012882378 26.605933617136962 28.880391599577383 23.295324654461876 88-89 21.45425674548499 26.55457401652157 28.727278203757823 23.263891034235616 90-91 21.396409616370814 26.80500033982292 28.650972511414963 23.147617532391305 92-93 21.42992851361454 26.417138814574077 28.57072796248355 23.58220470932783 94-95 21.10597686732695 26.193705243776606 28.745698150745447 23.954619738150992 96-97 21.240091072542928 26.186329541733343 28.77566435381127 23.79791503191246 98-99 21.20453370291665 26.200048424784995 28.587923229338468 24.00749464295989 100-101 20.859806385815784 26.186339653913393 28.811549967697502 24.14230399257332 102-103 21.182359792151946 25.902345704947205 28.682985375257186 24.232309127643667 104-105 21.06962696708356 25.97556261553617 28.730396743535668 24.224413673844598 106-107 20.955064597248054 25.885817953771728 28.776282213668296 24.382835235311926 108-109 21.070494873626693 25.974937730493714 28.381572087814483 24.572995308065106 110-111 20.877764591982892 26.284704267679277 28.301358531631944 24.536172608705893 112-113 20.791717897546476 26.12369399872721 28.563970120297316 24.520617983429 114-115 20.758314849025943 26.171887067575334 28.394908216918243 24.674889866480484 116-117 20.508274688135238 26.153505736828777 28.434451247767985 24.903768327268008 118-119 20.41567294206328 26.254255509765272 28.23032579750261 25.099745750668834 120-121 20.45969021883942 26.404306506485632 28.153129670176902 24.982873604498042 122-123 20.459397088626368 26.431744599871482 28.15925281745836 24.949605494043794 124-125 20.29751508254418 26.45916350238629 28.135919953135314 25.10740146193422 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 491.0 1 396.5 2 614.0 3 888.5 4 555.0 5 407.5 6 956.5 7 2178.5 8 3729.0 9 4313.0 10 3117.5 11 1834.0 12 1622.5 13 1699.0 14 1827.0 15 1956.5 16 2053.0 17 2185.0 18 2339.5 19 2485.5 20 2712.0 21 2999.5 22 3525.0 23 4319.0 24 5116.0 25 6135.0 26 7652.5 27 9153.0 28 10255.5 29 11921.0 30 14745.0 31 17609.0 32 20927.0 33 24602.0 34 28040.5 35 30712.0 36 35529.0 37 40209.5 38 41546.5 39 43150.0 40 43440.0 41 43324.0 42 42959.5 43 44170.0 44 47009.0 45 48544.5 46 47571.5 47 46172.0 48 46010.0 49 44955.5 50 44274.5 51 42447.0 52 40437.5 53 40014.0 54 39294.5 55 36356.5 56 32857.5 57 29785.0 58 26972.5 59 24149.0 60 20010.5 61 15820.0 62 12746.0 63 10087.0 64 7844.5 65 6001.0 66 4287.5 67 3547.5 68 2529.5 69 1315.5 70 888.5 71 655.5 72 503.5 73 461.0 74 369.0 75 276.5 76 190.5 77 112.5 78 66.5 79 29.5 80 12.5 81 11.0 82 8.0 83 4.0 84 2.0 85 2.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027803693566225307 2 0.0 3 0.0 4 0.0 5 0.05599354954309263 6 0.017377308478890817 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.930812053210091E-4 22-23 0.0 24-25 0.0 26-27 3.861624106420182E-4 28-29 3.4754616957781637E-4 30-31 2.703136874494127E-4 32-33 2.3169744638521091E-4 34-35 2.703136874494127E-4 36-37 0.0 38-39 0.0 40-41 1.5446496425680726E-4 42-43 0.0 44-45 1.1584872319260546E-4 46-47 0.0 48-49 3.4754616957781637E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.1584872319260546E-4 58-59 1.930812053210091E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 3.8616241064201814E-5 72-73 1.1584872319260546E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.3169744638521091E-4 82-83 0.0 84-85 3.8616241064201814E-5 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.8616241064201814E-5 100-101 3.8616241064201814E-5 102-103 0.0 104-105 0.001042638508733449 106-107 0.0 108-109 0.0013129521961828619 110-111 0.003745775383227576 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 5.406273748988254E-4 122-123 6.17859857027229E-4 124-125 3.8616241064201814E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1294792.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.82858018882709 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61646645424332 37.122853897801846 2 11.772469181021561 11.261209724899707 3 4.083252929044707 5.858885704442335 4 1.9485306894636785 3.7278182532561632 5 1.0594048241395564 2.533491429189452 6 0.6954110371638761 1.995631353311272 7 0.4434758753804763 1.4847575027211783 8 0.34505993472594926 1.3202981406393204 9 0.24678508326532222 1.062304212992565 >10 1.4956389894212703 13.873124893989864 >50 0.17954454601666395 6.016276889520271 >100 0.10746698283084268 8.863770761242414 >500 0.004545431297981215 1.3323607766548609 >1k 0.0012986946565660615 1.29120977364629 >5k 6.493473282830307E-4 2.25600668569252 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7916 0.6113723285284433 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6612 0.5106611718330049 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4298 0.3319452081878788 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1502 0.11600318815686225 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1423 0.10990182206871837 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.723248212840363E-5 0.0 18-19 0.0 0.0 0.0 7.723248212840363E-5 0.0 20-21 0.0 0.0 0.0 1.5446496425680726E-4 0.0 22-23 0.0 0.0 0.0 2.703136874494127E-4 0.0 24-25 0.0 0.0 0.0 5.792436159630272E-4 0.0 26-27 0.0 0.0 0.0 0.0015060334015038709 0.0 28-29 0.0 0.0 0.0 0.005135960061538842 0.0 30-31 0.0 0.0 0.0 0.02085277017466898 0.0 32-33 0.0 0.0 0.0 0.050703124517296985 0.0 34-35 0.0 0.0 0.0 0.09345130337536839 0.0 36-37 0.0 0.0 0.0 0.15863551829174105 0.0 38-39 0.0 0.0 0.0 0.26019623229059186 0.0 40-41 0.0 0.0 0.0 0.378477778670242 0.0 42-43 0.0 0.0 0.0 0.49115997009558293 0.0 44-45 0.0 0.0 0.0 0.621296702481943 0.0 46-47 0.0 0.0 0.0 0.7796619070862347 0.0 48-49 0.0 0.0 0.0 0.9598066716507362 0.0 50-51 0.0 0.0 0.0 1.157405977176257 0.0 52-53 0.0 0.0 0.0 1.3481315917923498 0.0 54-55 0.0 0.0 0.0 1.5436456203004036 0.0 56-57 0.0 0.0 0.0 1.7727557785343129 0.0 58-59 0.0 0.0 0.0 2.0288973055131634 0.0 60-61 0.0 0.0 0.0 2.320720239235337 0.0 62-63 0.0 0.0 0.0 2.602116787870175 0.0 64-65 0.0 0.0 0.0 2.8780298302738974 0.0 66-67 0.0 0.0 0.0 3.1877706998498603 0.0 68-69 0.0 0.0 0.0 3.5447778484884056 0.0 70-71 0.0 0.0 0.0 3.940246773226897 0.0 72-73 0.0 0.0 0.0 4.348188743829125 0.0 74-75 0.0 0.0 0.0 4.746322189201045 0.0 76-77 0.0 0.0 0.0 5.159129806177363 0.0 78-79 0.0 0.0 0.0 5.608545619682544 0.0 80-81 0.0 0.0 0.0 6.120403894988539 0.0 82-83 0.0 0.0 0.0 6.668059425761049 0.0 84-85 0.0 0.0 0.0 7.18906975019926 0.0 86-87 0.0 0.0 0.0 7.706874926629142 0.0 88-89 0.0 0.0 0.0 8.267968909291994 0.0 90-91 0.0 0.0 0.0 8.904943805646003 0.0 92-93 0.0 0.0 0.0 9.566362782593652 0.0 94-95 0.0 0.0 0.0 10.226932202237888 0.0 96-97 0.0 0.0 0.0 10.863675401145512 0.0 98-99 0.0 0.0 0.0 11.541737977991833 0.0 100-101 0.0 0.0 0.0 12.275794104381244 0.0 102-103 0.0 0.0 0.0 13.04958634282572 0.0 104-105 0.0 0.0 0.0 13.828591773813864 0.0 106-107 0.0 0.0 0.0 14.582303566904955 0.0 108-109 0.0 0.0 0.0 15.329103052845554 0.0 110-111 0.0 0.0 0.0 16.134328911516292 0.0 112-113 0.0 0.0 0.0 17.005009298790846 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3140 0.0 83.63887 1 GTATCAA 6410 0.0 61.596313 1 ATCAACG 8505 0.0 46.26921 3 TCAACGC 8510 0.0 46.242027 4 TATCAAC 8655 0.0 45.398533 2 CAACGCA 8730 0.0 45.076706 5 AACGCAG 9005 0.0 43.745956 6 TATAACG 110 9.584255E-9 43.297443 2 ACGCAGA 10315 0.0 38.18434 7 GTACATG 8725 0.0 38.154484 1 GTGGTAT 1150 0.0 37.802822 1 CGCAGAG 10420 0.0 37.742466 8 TACATGG 9180 0.0 35.603657 2 ACATGGG 9085 0.0 35.25513 3 GCAGAGT 11630 0.0 33.764534 9 CATGGGA 7080 0.0 32.62597 4 TGGTATC 1280 0.0 32.092537 2 CATGGGG 2395 0.0 30.574867 4 CAGAGTA 11370 0.0 28.388065 10-11 ATGGGAT 3280 0.0 28.314945 5 >>END_MODULE