FastQCFastQC Report
Thu 26 May 2016
SRR1364401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560882
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19800.047.50791
GGTATCA14900.039.956521
TCAACGC21900.039.40444
CAACGCA22050.039.136345
ATCAACG22300.038.4307143
GTACATG59700.038.1942831
TACATGG62950.036.0205272
AACGCAG24100.035.5558136
ACATGGG63300.034.7871483
TATCAAC26500.033.6872982
CATGGGG39250.030.6289774
ACGCAGA29050.029.2858377
GTATAGG5900.029.2630711
CGCAGAG30500.027.6993878
CATGGGA33300.025.9146044
GTATACG1150.001001510825.8848761
TACGACG1150.001003573425.8757514
CAATGCG1200.001288679524.789659
AGTACAT45650.024.3793242
GAGTACA48850.024.3747041
Produced by FastQC (version 0.11.5)