FastQCFastQC Report
Thu 26 May 2016
SRR1364401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560882
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31800.20373096749145675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.18989263762411251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.10910498038929273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.067.5629651
GTATCAA43300.051.4363941
ATCAACG52950.041.6070483
TCAACGC53450.041.217834
CAACGCA54350.040.535295
AACGCAG56550.038.9346056
TATCAAC57400.038.4851462
GTACATG59500.036.030671
ACGCAGA65850.033.5208637
ACATGGG61950.033.0634543
TACATGG64500.032.864072
CGCAGAG68250.032.2549328
CATGGGA30850.028.1792074
GCAGAGT78000.027.8416759
GAGTACT48450.026.83210612-13
CAGAGTA75400.025.9215610-11
CATGGGG40600.025.8117684
GTACTTT51900.024.81919114-15
GTGGTAT13150.024.4542751
AGTACAT45700.022.931242
Produced by FastQC (version 0.11.5)