FastQCFastQC Report
Thu 26 May 2016
SRR1364400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114265
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.051.539671
GTATCAA27300.045.7978481
ATCAACG30400.039.5478023
TCAACGC30250.039.3504034
CAACGCA30500.039.0269365
GTACATG81200.036.954131
AACGCAG32100.036.8918956
TACATGG84650.034.944112
ACATGGG83100.034.378333
TATCAAC35200.034.154922
ACGCAGA41800.028.4664927
CATGGGG52300.027.767244
CGCAGAG43050.027.5010858
TAAGGTG11100.025.20055
CATGGGA47150.024.4886364
GTAAGGT10600.023.58244
GTATAGG7350.023.490871
ATGGGGA32300.022.4797575
GCAGAGT54450.021.634029
GAGTACA64050.021.3794821
Produced by FastQC (version 0.11.5)