FastQCFastQC Report
Thu 26 May 2016
SRR1364400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114265
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34890.16502188703875814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33290.1574542453287549No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.071.0206451
GTATCAA53750.050.302161
ATCAACG66250.040.2661133
TCAACGC67050.039.6968734
CAACGCA67250.039.6673625
AACGCAG70400.038.1236576
TATCAAC70700.037.9001272
GTACATG84850.034.181131
TACATGG87700.032.318782
ACATGGG85700.032.2392353
ACGCAGA85250.031.3364777
CGCAGAG86250.030.9731568
GTGGTAT17500.029.9470391
TATAACG2154.0054147E-927.6954922
CATGGGG51700.026.6053524
GCAGAGT101550.026.0722399
TGGTATC17750.025.8309232
CAGAGTA94700.025.60211810-11
GAGTACA65350.025.0608841
GAGTACT61250.024.8189312-13
Produced by FastQC (version 0.11.5)